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Protein

Vesicle-associated membrane protein 3

Gene

Vamp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.
R-RNO-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-RNO-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 3
Short name:
VAMP-3
Alternative name(s):
Cellubrevin
Short name:
CEB
Synaptobrevin-3
Gene namesi
Name:Vamp3
Synonyms:Syb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61880. Vamp3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 81CytoplasmicSequence analysisAdd BLAST80
Transmembranei82 – 102Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini103VesicularSequence analysis1

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cell junction Source: UniProtKB-KW
  • cell surface Source: Ensembl
  • clathrin-coated vesicle membrane Source: RGD
  • cytosol Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • intracellular organelle Source: RGD
  • neuron projection Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: RGD
  • recycling endosome Source: RGD
  • secretory granule Source: Ensembl
  • SNARE complex Source: RGD
  • synapse Source: UniProtKB-KW

Keywords - Cellular componenti

Cell junction, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002067302 – 103Vesicle-associated membrane protein 3Add BLAST102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP63025.
PRIDEiP63025.

PTM databases

iPTMnetiP63025.
PhosphoSitePlusiP63025.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSRNOG00000030055.
GenevisibleiP63025. RN.

Interactioni

Subunit structurei

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BvesQ9ES833EBI-7705696,EBI-7705661From Mus musculus.

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

Protein-protein interaction databases

BioGridi248164. 1 interactor.
IntActiP63025. 3 interactors.
MINTiMINT-4139742.
STRINGi10116.ENSRNOP00000048364.

Structurei

Secondary structure

1103
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 75Combined sources61

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5KJ7X-ray3.50A/G14-76[»]
5KJ8X-ray4.10A/G14-76[»]
ProteinModelPortaliP63025.
SMRiP63025.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 78v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63025.
KOiK13505.
OMAiIIIWACS.
OrthoDBiEOG091G0MPE.
PhylomeDBiP63025.
TreeFamiTF313666.

Family and domain databases

InterProiView protein in InterPro
IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
PfamiView protein in Pfam
PF00957. Synaptobrevin. 1 hit.
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiView protein in PROSITE
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGVPSGSS AATGSNRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL
60 70 80 90 100
DDRADALQAG ASQFETSAAK LKRKYWWKNC KMWAIGISVL VIIVIIIIVW

CVS
Length:103
Mass (Da):11,480
Last modified:August 31, 2004 - v1
Checksum:iEB502BBE5D0F5981
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63830 mRNA. Translation: AAB27554.1.
BC088119 mRNA. Translation: AAH88119.1.
PIRiS35077.
RefSeqiNP_476438.1. NM_057097.2.
UniGeneiRn.219999.

Genome annotation databases

EnsembliENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055.
GeneIDi29528.
KEGGirno:29528.
UCSCiRGD:61880. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVAMP3_RAT
AccessioniPrimary (citable) accession number: P63025
Secondary accession number(s): Q64271
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: July 5, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families