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Protein

Vesicle-associated membrane protein 3

Gene

Vamp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 3
Short name:
VAMP-3
Alternative name(s):
Cellubrevin
Short name:
CEB
Synaptobrevin-3
Gene namesi
Name:Vamp3
Synonyms:Syb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61880. Vamp3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 8180CytoplasmicSequence analysisAdd
BLAST
Transmembranei82 – 10221Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini103 – 1031VesicularSequence analysis

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cell junction Source: UniProtKB-KW
  • cell surface Source: Ensembl
  • clathrin-coated vesicle membrane Source: RGD
  • cytosol Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • intracellular organelle Source: RGD
  • neuron projection Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • recycling endosome Source: RGD
  • secretory granule Source: Ensembl
  • SNARE complex Source: RGD
  • synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 103102Vesicle-associated membrane protein 3PRO_0000206730Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP63025.
PRIDEiP63025.

PTM databases

iPTMnetiP63025.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevisibleiP63025. RN.

Interactioni

Subunit structurei

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BvesQ9ES833EBI-7705696,EBI-7705661From a different organism.

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

Protein-protein interaction databases

BioGridi248164. 1 interaction.
IntActiP63025. 3 interactions.
MINTiMINT-4139742.
STRINGi10116.ENSRNOP00000048364.

Structurei

3D structure databases

ProteinModelPortaliP63025.
SMRiP63025. Positions 15-79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 7861v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63025.
KOiK13505.
OMAiFTHACAF.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP63025.
TreeFamiTF313666.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGVPSGSS AATGSNRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL
60 70 80 90 100
DDRADALQAG ASQFETSAAK LKRKYWWKNC KMWAIGISVL VIIVIIIIVW

CVS
Length:103
Mass (Da):11,480
Last modified:August 31, 2004 - v1
Checksum:iEB502BBE5D0F5981
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63830 mRNA. Translation: AAB27554.1.
BC088119 mRNA. Translation: AAH88119.1.
PIRiS35077.
RefSeqiNP_476438.1. NM_057097.2.
UniGeneiRn.219999.

Genome annotation databases

EnsembliENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055.
GeneIDi29528.
KEGGirno:29528.
UCSCiRGD:61880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63830 mRNA. Translation: AAB27554.1.
BC088119 mRNA. Translation: AAH88119.1.
PIRiS35077.
RefSeqiNP_476438.1. NM_057097.2.
UniGeneiRn.219999.

3D structure databases

ProteinModelPortaliP63025.
SMRiP63025. Positions 15-79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248164. 1 interaction.
IntActiP63025. 3 interactions.
MINTiMINT-4139742.
STRINGi10116.ENSRNOP00000048364.

PTM databases

iPTMnetiP63025.

Proteomic databases

PaxDbiP63025.
PRIDEiP63025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055.
GeneIDi29528.
KEGGirno:29528.
UCSCiRGD:61880. rat.

Organism-specific databases

CTDi9341.
RGDi61880. Vamp3.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63025.
KOiK13505.
OMAiFTHACAF.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP63025.
TreeFamiTF313666.

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiP63025.

Gene expression databases

GenevisibleiP63025. RN.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein."
    McMahon H.T., Ushkaryov Y.A., Edelmann L., Link E., Binz T., Niemann H., Jahn R., Suedhof T.C.
    Nature 364:346-349(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVAMP3_RAT
AccessioniPrimary (citable) accession number: P63025
Secondary accession number(s): Q64271
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: July 6, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.