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Protein

Vesicle-associated membrane protein 3

Gene

Vamp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.By similarity

GO - Molecular functioni

  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 3
Short name:
VAMP-3
Alternative name(s):
Cellubrevin
Short name:
CEB
Synaptobrevin-3
Gene namesi
Name:Vamp3
Synonyms:Syb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61880. Vamp3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8181CytoplasmicSequence analysisAdd
BLAST
Transmembranei82 – 10221Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini103 – 1031VesicularSequence analysis

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cell junction Source: UniProtKB-KW
  • cell surface Source: Ensembl
  • clathrin-coated vesicle membrane Source: RGD
  • cytosol Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • intracellular organelle Source: RGD
  • neuron projection Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • recycling endosome Source: RGD
  • secretory granule Source: Ensembl
  • SNARE complex Source: RGD
  • synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 103103Vesicle-associated membrane protein 3PRO_0000206730Add
BLAST

Proteomic databases

PaxDbiP63025.
PRIDEiP63025.

PTM databases

iPTMnetiP63025.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevisibleiP63025. RN.

Interactioni

Subunit structurei

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BvesQ9ES833EBI-7705696,EBI-7705661From a different organism.

GO - Molecular functioni

  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

Protein-protein interaction databases

BioGridi248164. 1 interaction.
IntActiP63025. 3 interactions.
MINTiMINT-4139742.
STRINGi10116.ENSRNOP00000048364.

Structurei

3D structure databases

ProteinModelPortaliP63025.
SMRiP63025. Positions 15-79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 7861v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63025.
KOiK13505.
OMAiFTHACAF.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP63025.
TreeFamiTF313666.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGVPSGSS AATGSNRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL
60 70 80 90 100
DDRADALQAG ASQFETSAAK LKRKYWWKNC KMWAIGISVL VIIVIIIIVW

CVS
Length:103
Mass (Da):11,480
Last modified:August 31, 2004 - v1
Checksum:iEB502BBE5D0F5981
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63830 mRNA. Translation: AAB27554.1.
BC088119 mRNA. Translation: AAH88119.1.
PIRiS35077.
RefSeqiNP_476438.1. NM_057097.2.
UniGeneiRn.219999.

Genome annotation databases

EnsembliENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055.
GeneIDi29528.
KEGGirno:29528.
UCSCiRGD:61880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63830 mRNA. Translation: AAB27554.1.
BC088119 mRNA. Translation: AAH88119.1.
PIRiS35077.
RefSeqiNP_476438.1. NM_057097.2.
UniGeneiRn.219999.

3D structure databases

ProteinModelPortaliP63025.
SMRiP63025. Positions 15-79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248164. 1 interaction.
IntActiP63025. 3 interactions.
MINTiMINT-4139742.
STRINGi10116.ENSRNOP00000048364.

PTM databases

iPTMnetiP63025.

Proteomic databases

PaxDbiP63025.
PRIDEiP63025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055.
GeneIDi29528.
KEGGirno:29528.
UCSCiRGD:61880. rat.

Organism-specific databases

CTDi9341.
RGDi61880. Vamp3.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63025.
KOiK13505.
OMAiFTHACAF.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP63025.
TreeFamiTF313666.

Miscellaneous databases

NextBioi302533.
PROiP63025.

Gene expression databases

GenevisibleiP63025. RN.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein."
    McMahon H.T., Ushkaryov Y.A., Edelmann L., Link E., Binz T., Niemann H., Jahn R., Suedhof T.C.
    Nature 364:346-349(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVAMP3_RAT
AccessioniPrimary (citable) accession number: P63025
Secondary accession number(s): Q64271
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.