P63020 (NFUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fe/S biogenesis protein NfuA

Gene names

Name:	nfuA
Synonyms:	gntY, yhgI
Ordered Locus Names:	b3414, JW3377

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in iron-sulfur cluster biogenesis under severe conditions such as iron starvation or oxidative stress. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins. Required for E.coli to sustain oxidative stress and iron starvation. Also necessary for the use of extracellular DNA as the sole source of carbon and energy. Ref.3 Ref.6 Ref.7
Cofactor	Binds 1 4Fe-4S cluster per subunit. The cluster is presumably bound at the interface of two monomers.
Subunit structure	Homodimer. Ref.7
Induction	Up-regulated under conditions of iron depletion or oxidative stress, via IscR. Is also induced by heat shock or the presence of misfolded proteins. Ref.4 Ref.5 Ref.7
Domain	Consits of two domains, an N-terminal domain that resembles the so-called Fe/S A-type scaffold, and a C-terminal domain that shares sequence identity with Nfu-type scaffold proteins. Both domains are important for NfuA to fulfill its function in vivo. The N-terminal domain does not possess the three conserved cysteine residues thought to act as Fe/S cluster ligands in A-type scaffold proteins, but may keep the ability to interact with apoproteins. Ref.7
Disruption phenotype	Cells express the stationary phase-specific competition-defective (SPCD) phenotype and are unable to catabolize dsDNA. Ref.6
Sequence similarities	Belongs to the NfuA family.
Caution	Was originally (Ref.3) thought to be involved in gluconate metabolism and was referred to as GntY.

Ontologies

Keywords
Biological process	Stress response
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon utilization Inferred from mutant phenotype Ref.6. Source: EcoCyc cellular response to iron ion starvation Inferred from mutant phenotype Ref.7. Source: EcoCyc iron-sulfur cluster assembly Inferred from direct assay Ref.7. Source: EcoliWiki protein maturation Inferred from electronic annotation. Source: HAMAP response to oxidative stress Inferred from mutant phenotype Ref.7. Source: EcoCyc
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from direct assay Ref.7. Source: EcoCyc iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Fe/S biogenesis protein NfuA HAMAP-Rule MF_01637		PRO_0000209464
Sites
	Metal binding	149	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	152	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Mutagenesis	39	1	C → S: No effect on activity in vivo. Still able to bind a Fe/S cluster in vitro. Ref.7
	Mutagenesis	44	1	C → S: No effect on activity in vivo. Ref.7
	Mutagenesis	149	1	C → S: Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro. Ref.7
	Mutagenesis	152	1	C → S: Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P63020 [UniParc]. Last modified August 31, 2004. Version 1. Checksum: 06874546ADA5A971	FASTA	191	20,998
	10 20 30 40 50 60 MIRISDAAQA HFAKLLANQE EGTQIRVFVI NPGTPNAECG VSYCPPDAVE ATDTALKFDL 70 80 90 100 110 120 LTAYVDELSA PYLEDAEIDF VTDQLGSQLT LKAPNAKMRK VADDAPLMER VEYMLQSQIN 130 140 150 160 170 180 PQLAGHGGRV SLMEITEDGY AILQFGGGCN GCSMVDVTLK EGIEKQLLNE FPELKGVRDL 190 TEHQRGEHSY Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The gluconate high affinity transport of GntI in Escherichia coli involves a multicomponent complex system." Porco A., Alonso G., Isturiz T. J. Basic Microbiol. 38:395-404(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Gene expression response to misfolded protein as a screen for soluble recombinant protein." Lesley S.A., Graziano J., Cho C.Y., Knuth M.W., Klock H.E. Protein Eng. 15:153-160(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY HEAT-SHOCK AND MISFOLDED PROTEINS.
[5]	"Global iron-dependent gene regulation in Escherichia coli. A new mechanism for iron homeostasis." McHugh J.P., Rodriguez-Quinones F., Abdul-Tehrani H., Svistunenko D.A., Poole R.K., Cooper C.E., Andrews S.C. J. Biol. Chem. 278:29478-29486(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY IRON DEPLETION CONDITIONS.
[6]	"Escherichia coli competence gene homologs are essential for competitive fitness and the use of DNA as a nutrient." Palchevskiy V., Finkel S.E. J. Bacteriol. 188:3902-3910(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, ROLE IN THE USE OF DNA AS A NUTRIENT. Strain: K12 / W3110 / ZK126.
[7]	"NfuA, a new factor required for maturing Fe/S proteins in Escherichia coli under oxidative stress and iron starvation conditions." Angelini S., Gerez C., Ollagnier-de Choudens S., Sanakis Y., Fontecave M., Barras F., Py B. J. Biol. Chem. 283:14084-14091(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN FE/S BIOGENESIS, IRON-SULFUR CLUSTER BINDING, SUBUNIT, INDUCTION BY OXIDATIVE STRESS AND IRON STARVATION, DOMAIN, MUTAGENESIS OF CYS-39; CYS-44; CYS-149 AND CYS-152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58212.1. U00096 Genomic DNA. Translation: AAC76439.1. AP009048 Genomic DNA. Translation: BAE77877.1.
PIR	A65137.
RefSeq	NP_417873.1. NC_000913.2. YP_492018.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P63020.
SMR	P63020. Positions 2-99, 106-186.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48001N.
IntAct	P63020. 12 interactions.
STRING	511145.b3414.
2D gel databases
SWISS-2DPAGE	P63020.
Proteomic databases
PaxDb	P63020.
PRIDE	P63020.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76439; AAC76439; b3414. BAE77877; BAE77877; BAE77877.
GeneID	12932225. 947925.
KEGG	ecj:Y75_p3762. eco:b3414.
PATRIC	32122266. VBIEscCol129921_3510.
Organism-specific databases
EchoBASE	EB2771.
EcoGene	EG12935. nfuA.
Phylogenomic databases
eggNOG	COG0694.
HOGENOM	HOG000137052.
KO	K07400.
OMA	CQGCSAV.
ProtClustDB	PRK11190.
Enzyme and pathway databases
BioCyc	EcoCyc:G7748-MONOMER. ECOL316407:JW3377-MONOMER.
Gene expression databases
Genevestigator	P63020.
Family and domain databases
Gene3D	2.60.300.12. 1 hit.
HAMAP	MF_01637. Fe_S_biogen_NfuA.
InterPro	IPR017726. Fe/S_biogenesis_protein_NfuA. IPR000361. FeS_biogenesis. IPR001075. NIF_FeS_clus_asmbl_NifU_C. [Graphical view]
Pfam	PF01521. Fe-S_biosyn. 1 hit. PF01106. NifU. 1 hit. [Graphical view]
ProDom	PD002830. NIF_FeS_clus_asmbl_NifU_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF89360. HesB_yadR_yfhF-like. 1 hit.
TIGRFAMs	TIGR03341. YhgI_GntY. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NFUA_ECOLI

Accession

Primary (citable) accession number: P63020
Secondary accession number(s): P46847, Q2M779

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program