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Protein

Fe/S biogenesis protein NfuA

Gene

nfuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in iron-sulfur cluster biogenesis under severe conditions such as iron starvation or oxidative stress. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins. Required for E.coli to sustain oxidative stress and iron starvation. Also necessary for the use of extracellular DNA as the sole source of carbon and energy.2 Publications

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit. The cluster is presumably bound at the interface of two monomers.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi149 – 1491Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi152 – 1521Iron-sulfur (4Fe-4S)Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • carbon utilization Source: EcoCyc
  • cellular response to iron ion starvation Source: EcoCyc
  • iron-sulfur cluster assembly Source: EcoliWiki
  • protein maturation by iron-sulfur cluster transfer Source: EcoCyc
  • response to oxidative stress Source: EcoCyc
  • sulfur compound metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7748-MONOMER.
ECOL316407:JW3377-MONOMER.
MetaCyc:G7748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fe/S biogenesis protein NfuA
Gene namesi
Name:nfuA
Synonyms:gntY, yhgI
Ordered Locus Names:b3414, JW3377
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12935. nfuA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells express the stationary phase-specific competition-defective (SPCD) phenotype and are unable to catabolize dsDNA.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391C → S: No effect on activity in vivo. Still able to bind a Fe/S cluster in vitro. 1 Publication
Mutagenesisi44 – 441C → S: No effect on activity in vivo. 1 Publication
Mutagenesisi149 – 1491C → S: Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro. 1 Publication
Mutagenesisi152 – 1521C → S: Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Fe/S biogenesis protein NfuAPRO_0000209464Add
BLAST

Proteomic databases

EPDiP63020.
PaxDbiP63020.
PRIDEiP63020.

2D gel databases

SWISS-2DPAGEP63020.

Expressioni

Inductioni

Up-regulated under conditions of iron depletion or oxidative stress, via IscR. Is also induced by heat shock or the presence of misfolded proteins.3 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4261219. 422 interactions.
DIPiDIP-48001N.
IntActiP63020. 13 interactions.
STRINGi511145.b3414.

Structurei

3D structure databases

ProteinModelPortaliP63020.
SMRiP63020. Positions 2-99, 106-186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consits of two domains, an N-terminal domain that resembles the so-called Fe/S A-type scaffold, and a C-terminal domain that shares sequence identity with Nfu-type scaffold proteins. Both domains are important for NfuA to fulfill its function in vivo. The N-terminal domain does not possess the three conserved cysteine residues thought to act as Fe/S cluster ligands in A-type scaffold proteins, but may keep the ability to interact with apoproteins.1 Publication

Sequence similaritiesi

Belongs to the NfuA family.Curated

Phylogenomic databases

eggNOGiENOG4107RUK. Bacteria.
COG0316. LUCA.
COG0694. LUCA.
HOGENOMiHOG000137052.
InParanoidiP63020.
KOiK07400.
OMAiETCLAYC.
PhylomeDBiP63020.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01637. Fe_S_biogen_NfuA. 1 hit.
InterProiIPR017726. Fe/S_biogenesis_protein_NfuA.
IPR000361. FeS_biogenesis.
IPR001075. NIF_FeS_clus_asmbl_NifU_C.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
PF01106. NifU. 1 hit.
[Graphical view]
ProDomiPD002830. NIF_FeS_clus_asmbl_NifU_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR03341. YhgI_GntY. 1 hit.

Sequencei

Sequence statusi: Complete.

P63020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRISDAAQA HFAKLLANQE EGTQIRVFVI NPGTPNAECG VSYCPPDAVE
60 70 80 90 100
ATDTALKFDL LTAYVDELSA PYLEDAEIDF VTDQLGSQLT LKAPNAKMRK
110 120 130 140 150
VADDAPLMER VEYMLQSQIN PQLAGHGGRV SLMEITEDGY AILQFGGGCN
160 170 180 190
GCSMVDVTLK EGIEKQLLNE FPELKGVRDL TEHQRGEHSY Y
Length:191
Mass (Da):20,998
Last modified:August 31, 2004 - v1
Checksum:i06874546ADA5A971
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58212.1.
U00096 Genomic DNA. Translation: AAC76439.1.
AP009048 Genomic DNA. Translation: BAE77877.1.
PIRiA65137.
RefSeqiNP_417873.1. NC_000913.3.
WP_000619389.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76439; AAC76439; b3414.
BAE77877; BAE77877; BAE77877.
GeneIDi947925.
KEGGiecj:JW3377.
eco:b3414.
PATRICi32122266. VBIEscCol129921_3510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58212.1.
U00096 Genomic DNA. Translation: AAC76439.1.
AP009048 Genomic DNA. Translation: BAE77877.1.
PIRiA65137.
RefSeqiNP_417873.1. NC_000913.3.
WP_000619389.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP63020.
SMRiP63020. Positions 2-99, 106-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261219. 422 interactions.
DIPiDIP-48001N.
IntActiP63020. 13 interactions.
STRINGi511145.b3414.

2D gel databases

SWISS-2DPAGEP63020.

Proteomic databases

EPDiP63020.
PaxDbiP63020.
PRIDEiP63020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76439; AAC76439; b3414.
BAE77877; BAE77877; BAE77877.
GeneIDi947925.
KEGGiecj:JW3377.
eco:b3414.
PATRICi32122266. VBIEscCol129921_3510.

Organism-specific databases

EchoBASEiEB2771.
EcoGeneiEG12935. nfuA.

Phylogenomic databases

eggNOGiENOG4107RUK. Bacteria.
COG0316. LUCA.
COG0694. LUCA.
HOGENOMiHOG000137052.
InParanoidiP63020.
KOiK07400.
OMAiETCLAYC.
PhylomeDBiP63020.

Enzyme and pathway databases

BioCyciEcoCyc:G7748-MONOMER.
ECOL316407:JW3377-MONOMER.
MetaCyc:G7748-MONOMER.

Miscellaneous databases

PROiP63020.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01637. Fe_S_biogen_NfuA. 1 hit.
InterProiIPR017726. Fe/S_biogenesis_protein_NfuA.
IPR000361. FeS_biogenesis.
IPR001075. NIF_FeS_clus_asmbl_NifU_C.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
PF01106. NifU. 1 hit.
[Graphical view]
ProDomiPD002830. NIF_FeS_clus_asmbl_NifU_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR03341. YhgI_GntY. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNFUA_ECOLI
AccessioniPrimary (citable) accession number: P63020
Secondary accession number(s): P46847, Q2M779
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in gluconate metabolism and was referred to as GntY.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.