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Protein

Paired mesoderm homeobox protein 1

Gene

Prrx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi94 – 15360HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. HMG box domain binding Source: UniProtKB
  3. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. artery morphogenesis Source: MGI
  2. cartilage development Source: MGI
  3. embryonic cranial skeleton morphogenesis Source: MGI
  4. embryonic limb morphogenesis Source: MGI
  5. embryonic skeletal system morphogenesis Source: MGI
  6. inner ear morphogenesis Source: MGI
  7. middle ear morphogenesis Source: MGI
  8. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  9. palate development Source: MGI
  10. positive regulation of mesenchymal cell proliferation Source: MGI
  11. positive regulation of smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Paired mesoderm homeobox protein 1
Alternative name(s):
Homeobox protein K-2
Homeobox protein mHox
Paired-related homeobox protein 1
Short name:
PRX-1
Gene namesi
Name:Prrx1
Synonyms:Pmx, Pmx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:97712. Prrx1.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Paired mesoderm homeobox protein 1PRO_0000049252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei160 – 1601N6-acetyllysine1 Publication
Modified residuei197 – 1971PhosphoserineSequence Analysis

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP63013.
PRIDEiP63013.

PTM databases

PhosphoSiteiP63013.

Expressioni

Tissue specificityi

Expressed exclusively in mesodermally derived cell types. During embryogenesis, highest levels of expression are found in the mesenchyme and precartilage elements of the face and hind limbs. In the adult, expression is restricted to skeletal muscle, heart and uterus.

Developmental stagei

Expressed during cardiogenesis.

Gene expression databases

BgeeiP63013.
CleanExiMM_PRRX1.
ExpressionAtlasiP63013. baseline and differential.
GenevestigatoriP63013.

Interactioni

Protein-protein interaction databases

BioGridi202269. 2 interactions.
IntActiP63013. 1 interaction.
STRINGi10090.ENSMUSP00000027878.

Structurei

3D structure databases

ProteinModelPortaliP63013.
SMRiP63013. Positions 94-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi222 – 23514OARAdd
BLAST

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG267226.
GeneTreeiENSGT00760000118958.
HOGENOMiHOG000231518.
HOVERGENiHBG021349.
InParanoidiP63013.
KOiK09329.
OMAiCANNTTA.
OrthoDBiEOG7DFXD8.
PhylomeDBiP63013.
TreeFamiTF351612.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P63013-1) [UniParc]FASTAAdd to Basket

Also known as: K2-a, PMX1-B, Prx1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSSYGHVLE RQPALGGRLD SPGNLDTLQA KKNFSVSHLL DLEEAGDMVA
60 70 80 90 100
AQADESVGEA GRSLLESPGL TSGSDTPQQD NDQLNSEEKK KRKQRRNRTT
110 120 130 140 150
FNSSQLQALE RVFERTHYPD AFVREDLARR VNLTEARVQV WFQNRRAKFR
160 170 180 190 200
RNERAMLANK NASLLKSYSG DVTAVEQPIV PRPAPRPTDY LSWGTASPYS
210 220 230 240
AMATYSATCA NNSPAQGINM ANSIANLRLK AKEYSLQRNQ VPTVN
Length:245
Mass (Da):27,269
Last modified:August 31, 2004 - v1
Checksum:iFF867F0D856E1115
GO
Isoform 2 (identifier: P63013-2) [UniParc] [UniParc]FASTAAdd to Basket

Also known as: K2-b, PMX1-A, Prx1b

The sequence of this isoform differs from the canonical sequence as follows:
     200-245: SAMATYSATCANNSPAQGINMANSIANLRLKAKEYSLQRNQVPTVN → RSSSLPRCCLHEGLHNGF

Show »
Length:217
Mass (Da):24,369
Checksum:iA4C1F7A6DF8CE652
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei200 – 24546SAMAT…VPTVN → RSSSLPRCCLHEGLHNGF in isoform 2. 3 PublicationsVSP_002279Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06502 mRNA. Translation: AAA39672.1.
U03873 mRNA. Translation: AAC52139.1.
X59725 mRNA. Translation: CAA42410.1.
BC092372 mRNA. Translation: AAH92372.1.
CCDSiCCDS15427.1.
CCDS15428.1. [P63013-2]
PIRiI48902.
S26076.
RefSeqiNP_035257.1. NM_011127.2. [P63013-1]
NP_783617.2. NM_175686.3. [P63013-2]
XP_006496766.1. XM_006496703.1. [P63013-2]
UniGeneiMm.288642.

Genome annotation databases

EnsembliENSMUST00000027878; ENSMUSP00000027878; ENSMUSG00000026586. [P63013-1]
ENSMUST00000075805; ENSMUSP00000075203; ENSMUSG00000026586. [P63013-2]
GeneIDi18933.
KEGGimmu:18933.
UCSCiuc007dhh.1. mouse. [P63013-2]
uc007dhi.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06502 mRNA. Translation: AAA39672.1.
U03873 mRNA. Translation: AAC52139.1.
X59725 mRNA. Translation: CAA42410.1.
BC092372 mRNA. Translation: AAH92372.1.
CCDSiCCDS15427.1.
CCDS15428.1. [P63013-2]
PIRiI48902.
S26076.
RefSeqiNP_035257.1. NM_011127.2. [P63013-1]
NP_783617.2. NM_175686.3. [P63013-2]
XP_006496766.1. XM_006496703.1. [P63013-2]
UniGeneiMm.288642.

3D structure databases

ProteinModelPortaliP63013.
SMRiP63013. Positions 94-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202269. 2 interactions.
IntActiP63013. 1 interaction.
STRINGi10090.ENSMUSP00000027878.

PTM databases

PhosphoSiteiP63013.

Proteomic databases

MaxQBiP63013.
PRIDEiP63013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027878; ENSMUSP00000027878; ENSMUSG00000026586. [P63013-1]
ENSMUST00000075805; ENSMUSP00000075203; ENSMUSG00000026586. [P63013-2]
GeneIDi18933.
KEGGimmu:18933.
UCSCiuc007dhh.1. mouse. [P63013-2]
uc007dhi.1. mouse.

Organism-specific databases

CTDi5396.
MGIiMGI:97712. Prrx1.

Phylogenomic databases

eggNOGiNOG267226.
GeneTreeiENSGT00760000118958.
HOGENOMiHOG000231518.
HOVERGENiHBG021349.
InParanoidiP63013.
KOiK09329.
OMAiCANNTTA.
OrthoDBiEOG7DFXD8.
PhylomeDBiP63013.
TreeFamiTF351612.

Miscellaneous databases

NextBioi295248.
PROiP63013.
SOURCEiSearch...

Gene expression databases

BgeeiP63013.
CleanExiMM_PRRX1.
ExpressionAtlasiP63013. baseline and differential.
GenevestigatoriP63013.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MHox: a mesodermally restricted homeodomain protein that binds an essential site in the muscle creatine kinase enhancer."
    Cserjesi P., Lilly B., Bryson L., Wang Y., Sassoon D.A., Olson E.N.
    Development 115:1087-1101(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Genomic organization and chromosome localization of the murine homeobox gene Pmx."
    Kern M.J., Argao E.A., Birkenmeier E.H., Rowe L.B., Potter S.S.
    Genomics 19:334-340(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "A novel murine homeobox gene isolated by a tissue specific PCR cloning strategy."
    Kern M.J., Witte D.P., Valerius M.T., Aronow B.J., Potter S.S.
    Nucleic Acids Res. 20:5189-5195(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: CD-1.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiPRRX1_MOUSE
AccessioniPrimary (citable) accession number: P63013
Secondary accession number(s): P43271, Q02810, Q569N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: February 4, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.