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Protein

Ras-related protein Rab-3A

Gene

Rab3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 37GTP1 Publication9
Nucleotide bindingi48 – 54GTP1 Publication7
Nucleotide bindingi77 – 81GTP1 Publication5
Nucleotide bindingi135 – 138GTP1 Publication4
Nucleotide bindingi165 – 167GTPBy similarity3

GO - Molecular functioni

  • ATPase activator activity Source: RGD
  • ATPase binding Source: RGD
  • GTPase activity Source: Ensembl
  • GTP binding Source: RGD
  • protein C-terminus binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-3A
Gene namesi
Name:Rab3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi3528. Rab3a.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: Ensembl
  • axon Source: ParkinsonsUK-UCL
  • cytosol Source: RGD
  • endosome Source: Ensembl
  • extracellular vesicle Source: Ensembl
  • intracellular organelle Source: RGD
  • membrane Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: RGD
  • synaptic vesicle Source: ParkinsonsUK-UCL
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36T → N: No significant effect on interaction with RAB3IP. 1 Publication1
Mutagenesisi51F → L: Disrupts the interaction with RAB3IP. 1 Publication1
Mutagenesisi52V → A: No significant effect on interaction with RAB3IP. 1
Mutagenesisi55V → E: Disrupts the interaction with RAB3IP. 1 Publication1
Mutagenesisi56G → D: Disrupts the interaction with RAB3IP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001210791 – 220Ras-related protein Rab-3AAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Lipidationi218S-geranylgeranyl cysteineBy similarity1
Modified residuei220Cysteine methyl esterBy similarity1
Lipidationi220S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP63012.
PRIDEiP63012.

PTM databases

iPTMnetiP63012.
PhosphoSitePlusiP63012.
SwissPalmiP63012.

Expressioni

Tissue specificityi

Detected in brain.

Gene expression databases

BgeeiENSRNOG00000019433.
GenevisibleiP63012. RN.

Interactioni

Subunit structurei

Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4 and RIMS1. Interacts with SGSM1 and SGSM3 (By similarity). Heterodimer with RIMS2. Interacts with RPH3A. Interacts with RAB3IP.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rph3aP477092EBI-440126,EBI-1027524

GO - Molecular functioni

  • ATPase binding Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi247563. 3 interactors.
IntActiP63012. 4 interactors.
MINTiMINT-1506235.
STRINGi10116.ENSRNOP00000026392.

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 28Combined sources9
Helixi35 – 44Combined sources10
Beta strandi56 – 66Combined sources11
Beta strandi69 – 78Combined sources10
Helixi82 – 84Combined sources3
Helixi85 – 89Combined sources5
Turni90 – 94Combined sources5
Beta strandi97 – 103Combined sources7
Helixi107 – 111Combined sources5
Helixi113 – 123Combined sources11
Beta strandi129 – 135Combined sources7
Helixi140 – 142Combined sources3
Helixi147 – 157Combined sources11
Beta strandi160 – 163Combined sources4
Turni166 – 169Combined sources4
Helixi172 – 184Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZBDX-ray2.60A19-217[»]
3RABX-ray2.00A18-186[»]
ProteinModelPortaliP63012.
SMRiP63012.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63012.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi51 – 59Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0093. Eukaryota.
ENOG410ZZXQ. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP63012.
KOiK07882.
OMAiQLTEQPA.
OrthoDBiEOG091G0J64.
PhylomeDBiP63012.
TreeFamiTF313199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA
60 70 80 90 100
FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL
110 120 130 140 150
MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG
160 170 180 190 200
RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG
210 220
AKQGPQLTDQ QAPPHQDCAC
Length:220
Mass (Da):24,970
Last modified:August 31, 2004 - v1
Checksum:i1A3E9F8C9D09EB40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196P → L in CAA30005 (PubMed:3344209).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06889 mRNA. Translation: CAA30005.1.
BC087580 mRNA. Translation: AAH87580.1.
PIRiS01765.
RefSeqiNP_037150.2. NM_013018.2.
UniGeneiRn.44409.

Genome annotation databases

EnsembliENSRNOT00000026391; ENSRNOP00000026392; ENSRNOG00000019433.
GeneIDi25531.
KEGGirno:25531.
UCSCiRGD:3528. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06889 mRNA. Translation: CAA30005.1.
BC087580 mRNA. Translation: AAH87580.1.
PIRiS01765.
RefSeqiNP_037150.2. NM_013018.2.
UniGeneiRn.44409.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZBDX-ray2.60A19-217[»]
3RABX-ray2.00A18-186[»]
ProteinModelPortaliP63012.
SMRiP63012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247563. 3 interactors.
IntActiP63012. 4 interactors.
MINTiMINT-1506235.
STRINGi10116.ENSRNOP00000026392.

PTM databases

iPTMnetiP63012.
PhosphoSitePlusiP63012.
SwissPalmiP63012.

Proteomic databases

PaxDbiP63012.
PRIDEiP63012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026391; ENSRNOP00000026392; ENSRNOG00000019433.
GeneIDi25531.
KEGGirno:25531.
UCSCiRGD:3528. rat.

Organism-specific databases

CTDi5864.
RGDi3528. Rab3a.

Phylogenomic databases

eggNOGiKOG0093. Eukaryota.
ENOG410ZZXQ. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP63012.
KOiK07882.
OMAiQLTEQPA.
OrthoDBiEOG091G0J64.
PhylomeDBiP63012.
TreeFamiTF313199.

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

EvolutionaryTraceiP63012.
PROiP63012.

Gene expression databases

BgeeiENSRNOG00000019433.
GenevisibleiP63012. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB3A_RAT
AccessioniPrimary (citable) accession number: P63012
Secondary accession number(s): P05713
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.