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P63012

- RAB3A_RAT

UniProt

P63012 - RAB3A_RAT

Protein

Ras-related protein Rab-3A

Gene

Rab3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 368GTP1 Publication
    Nucleotide bindingi48 – 547GTP1 Publication
    Nucleotide bindingi77 – 815GTP1 Publication
    Nucleotide bindingi135 – 1384GTP1 Publication

    GO - Molecular functioni

    1. ATPase activator activity Source: RGD
    2. ATPase binding Source: RGD
    3. GTPase activity Source: Ensembl
    4. GTPase binding Source: RGD
    5. GTP binding Source: RGD
    6. protein binding Source: UniProtKB
    7. protein C-terminus binding Source: RGD

    GO - Biological processi

    1. axonogenesis Source: Ensembl
    2. lung development Source: Ensembl
    3. maintenance of presynaptic active zone structure Source: Ensembl
    4. mitochondrion organization Source: Ensembl
    5. neuromuscular synaptic transmission Source: Ensembl
    6. positive regulation of ATPase activity Source: GOC
    7. post-embryonic development Source: Ensembl
    8. protein transport Source: UniProtKB-KW
    9. Rab protein signal transduction Source: RGD
    10. regulation of synaptic vesicle fusion to presynaptic membrane Source: Ensembl
    11. respiratory system process Source: Ensembl
    12. response to electrical stimulus Source: Ensembl
    13. sensory perception of touch Source: Ensembl
    14. synaptic vesicle exocytosis Source: ParkinsonsUK-UCL
    15. synaptic vesicle maturation Source: Ensembl

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-3A
    Gene namesi
    Name:Rab3a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 16

    Organism-specific databases

    RGDi3528. Rab3a.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. acrosomal vesicle Source: Ensembl
    2. axon Source: ParkinsonsUK-UCL
    3. cytosol Source: RGD
    4. intracellular organelle Source: RGD
    5. membrane Source: RGD
    6. plasma membrane Source: UniProtKB-SubCell
    7. protein complex Source: RGD
    8. synaptic vesicle Source: ParkinsonsUK-UCL
    9. terminal bouton Source: ParkinsonsUK-UCL

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi36 – 361T → N: No significant effect on interaction with RAB3IP. 1 Publication
    Mutagenesisi51 – 511F → L: Disrupts the interaction with RAB3IP. 1 Publication
    Mutagenesisi52 – 521V → A: No significant effect on interaction with RAB3IP.
    Mutagenesisi55 – 551V → E: Disrupts the interaction with RAB3IP. 1 Publication
    Mutagenesisi56 – 561G → D: Disrupts the interaction with RAB3IP. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220Ras-related protein Rab-3APRO_0000121079Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi218 – 2181S-geranylgeranyl cysteineBy similarity
    Modified residuei220 – 2201Cysteine methyl esterBy similarity
    Lipidationi220 – 2201S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PaxDbiP63012.
    PRIDEiP63012.

    PTM databases

    PhosphoSiteiP63012.

    Expressioni

    Tissue specificityi

    Detected in brain.

    Gene expression databases

    GenevestigatoriP63012.

    Interactioni

    Subunit structurei

    Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4 and RIMS1. Interacts with SGSM1 and SGSM3 By similarity. Heterodimer with RIMS2. Interacts with RPH3A. Interacts with RAB3IP.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rph3aP477092EBI-440126,EBI-1027524

    Protein-protein interaction databases

    BioGridi247563. 2 interactions.
    IntActiP63012. 4 interactions.
    MINTiMINT-1506235.

    Structurei

    Secondary structure

    1
    220
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi20 – 289
    Helixi35 – 4410
    Beta strandi56 – 6611
    Beta strandi69 – 7810
    Helixi82 – 843
    Helixi85 – 895
    Turni90 – 945
    Beta strandi97 – 1037
    Helixi107 – 1115
    Helixi113 – 12311
    Beta strandi129 – 1357
    Helixi140 – 1423
    Helixi147 – 15711
    Beta strandi160 – 1634
    Turni166 – 1694
    Helixi172 – 18413

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZBDX-ray2.60A19-217[»]
    3RABX-ray2.00A18-186[»]
    ProteinModelPortaliP63012.
    SMRiP63012. Positions 18-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63012.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi51 – 599Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00740000115041.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP63012.
    KOiK07882.
    OMAiQLTEQPA.
    OrthoDBiEOG7JQBPC.
    PhylomeDBiP63012.
    TreeFamiTF313199.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63012-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA    50
    FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL 100
    MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG 150
    RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG 200
    AKQGPQLTDQ QAPPHQDCAC 220
    Length:220
    Mass (Da):24,970
    Last modified:August 31, 2004 - v1
    Checksum:i1A3E9F8C9D09EB40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti196 – 1961P → L in CAA30005. (PubMed:3344209)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06889 mRNA. Translation: CAA30005.1.
    BC087580 mRNA. Translation: AAH87580.1.
    PIRiS01765.
    RefSeqiNP_037150.2. NM_013018.2.
    UniGeneiRn.44409.

    Genome annotation databases

    EnsembliENSRNOT00000026391; ENSRNOP00000026392; ENSRNOG00000019433.
    GeneIDi25531.
    KEGGirno:25531.
    UCSCiRGD:3528. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06889 mRNA. Translation: CAA30005.1 .
    BC087580 mRNA. Translation: AAH87580.1 .
    PIRi S01765.
    RefSeqi NP_037150.2. NM_013018.2.
    UniGenei Rn.44409.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZBD X-ray 2.60 A 19-217 [» ]
    3RAB X-ray 2.00 A 18-186 [» ]
    ProteinModelPortali P63012.
    SMRi P63012. Positions 18-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247563. 2 interactions.
    IntActi P63012. 4 interactions.
    MINTi MINT-1506235.

    PTM databases

    PhosphoSitei P63012.

    Proteomic databases

    PaxDbi P63012.
    PRIDEi P63012.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026391 ; ENSRNOP00000026392 ; ENSRNOG00000019433 .
    GeneIDi 25531.
    KEGGi rno:25531.
    UCSCi RGD:3528. rat.

    Organism-specific databases

    CTDi 5864.
    RGDi 3528. Rab3a.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00740000115041.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P63012.
    KOi K07882.
    OMAi QLTEQPA.
    OrthoDBi EOG7JQBPC.
    PhylomeDBi P63012.
    TreeFami TF313199.

    Enzyme and pathway databases

    Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.

    Miscellaneous databases

    EvolutionaryTracei P63012.
    NextBioi 296339.
    PROi P63012.

    Gene expression databases

    Genevestigatori P63012.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library."
      Touchot N., Chardin P., Tavitian A.
      Proc. Natl. Acad. Sci. U.S.A. 84:8210-8214(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Complete coding sequences of the ras related rab 3 and 4 cDNAs."
      Zahraoui A., Touchot N., Chardin P., Tavitian A.
      Nucleic Acids Res. 16:1204-1204(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A."
      Brondyk W.H., McKiernan C.J., Fortner K.A., Stabila P., Holz R.W., Macara I.G.
      Mol. Cell. Biol. 15:1137-1143(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAB3IP, MUTAGENESIS OF THR-36; PHE-51; VAL-55 AND GLY-56.
    5. "The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins."
      Wang Y., Sugita S., Suedhof T.C.
      J. Biol. Chem. 275:20033-20044(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RIMS2.
    6. "Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A."
      Ostermeier C., Brunger A.T.
      Cell 96:363-374(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 19-217 IN COMPLEX WITH GTP AND RPH3A, INTERACTION WITH RPH3A.
    7. "Structural basis of activation and GTP hydrolysis in rab proteins."
      Dumas J.J., Zhu Z., Connolly J.L., Lambright D.G.
      Structure 7:413-423(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-186 IN COMPLEX WITH GTP ANALOG.

    Entry informationi

    Entry nameiRAB3A_RAT
    AccessioniPrimary (citable) accession number: P63012
    Secondary accession number(s): P05713
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3