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P63012

- RAB3A_RAT

UniProt

P63012 - RAB3A_RAT

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Protein

Ras-related protein Rab-3A

Gene

Rab3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 368GTP1 Publication
Nucleotide bindingi48 – 547GTP1 Publication
Nucleotide bindingi77 – 815GTP1 Publication
Nucleotide bindingi135 – 1384GTP1 Publication

GO - Molecular functioni

  1. ATPase activator activity Source: RGD
  2. ATPase binding Source: RGD
  3. GTPase activity Source: Ensembl
  4. GTPase binding Source: RGD
  5. GTP binding Source: RGD
  6. protein C-terminus binding Source: RGD

GO - Biological processi

  1. axonogenesis Source: Ensembl
  2. lung development Source: Ensembl
  3. maintenance of presynaptic active zone structure Source: Ensembl
  4. mitochondrion organization Source: Ensembl
  5. neuromuscular synaptic transmission Source: Ensembl
  6. positive regulation of ATPase activity Source: GOC
  7. post-embryonic development Source: Ensembl
  8. protein transport Source: UniProtKB-KW
  9. Rab protein signal transduction Source: RGD
  10. regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
  11. regulation of synaptic vesicle fusion to presynaptic membrane Source: ParkinsonsUK-UCL
  12. respiratory system process Source: Ensembl
  13. response to electrical stimulus Source: Ensembl
  14. sensory perception of touch Source: Ensembl
  15. synaptic vesicle exocytosis Source: ParkinsonsUK-UCL
  16. synaptic vesicle maturation Source: Ensembl
  17. synaptic vesicle recycling Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.
REACT_245524. Acetylcholine Neurotransmitter Release Cycle.
REACT_246747. Norepinephrine Neurotransmitter Release Cycle.
REACT_262380. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-3A
Gene namesi
Name:Rab3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 16

Organism-specific databases

RGDi3528. Rab3a.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. acrosomal vesicle Source: Ensembl
  2. axon Source: ParkinsonsUK-UCL
  3. cytosol Source: RGD
  4. intracellular organelle Source: RGD
  5. membrane Source: RGD
  6. plasma membrane Source: UniProtKB-KW
  7. protein complex Source: RGD
  8. synaptic vesicle Source: ParkinsonsUK-UCL
  9. terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 361T → N: No significant effect on interaction with RAB3IP. 1 Publication
Mutagenesisi51 – 511F → L: Disrupts the interaction with RAB3IP. 1 Publication
Mutagenesisi52 – 521V → A: No significant effect on interaction with RAB3IP.
Mutagenesisi55 – 551V → E: Disrupts the interaction with RAB3IP. 1 Publication
Mutagenesisi56 – 561G → D: Disrupts the interaction with RAB3IP. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ras-related protein Rab-3APRO_0000121079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi218 – 2181S-geranylgeranyl cysteineBy similarity
Modified residuei220 – 2201Cysteine methyl esterBy similarity
Lipidationi220 – 2201S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP63012.
PRIDEiP63012.

PTM databases

PhosphoSiteiP63012.

Expressioni

Tissue specificityi

Detected in brain.

Gene expression databases

GenevestigatoriP63012.

Interactioni

Subunit structurei

Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4 and RIMS1. Interacts with SGSM1 and SGSM3 (By similarity). Heterodimer with RIMS2. Interacts with RPH3A. Interacts with RAB3IP.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rph3aP477092EBI-440126,EBI-1027524

Protein-protein interaction databases

BioGridi247563. 3 interactions.
IntActiP63012. 4 interactions.
MINTiMINT-1506235.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 289Combined sources
Helixi35 – 4410Combined sources
Beta strandi56 – 6611Combined sources
Beta strandi69 – 7810Combined sources
Helixi82 – 843Combined sources
Helixi85 – 895Combined sources
Turni90 – 945Combined sources
Beta strandi97 – 1037Combined sources
Helixi107 – 1115Combined sources
Helixi113 – 12311Combined sources
Beta strandi129 – 1357Combined sources
Helixi140 – 1423Combined sources
Helixi147 – 15711Combined sources
Beta strandi160 – 1634Combined sources
Turni166 – 1694Combined sources
Helixi172 – 18413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZBDX-ray2.60A19-217[»]
3RABX-ray2.00A18-186[»]
ProteinModelPortaliP63012.
SMRiP63012. Positions 18-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63012.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 599Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP63012.
KOiK07882.
OMAiQLTEQPA.
OrthoDBiEOG7JQBPC.
PhylomeDBiP63012.
TreeFamiTF313199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63012-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA
60 70 80 90 100
FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL
110 120 130 140 150
MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG
160 170 180 190 200
RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG
210 220
AKQGPQLTDQ QAPPHQDCAC
Length:220
Mass (Da):24,970
Last modified:August 31, 2004 - v1
Checksum:i1A3E9F8C9D09EB40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961P → L in CAA30005. (PubMed:3344209)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06889 mRNA. Translation: CAA30005.1.
BC087580 mRNA. Translation: AAH87580.1.
PIRiS01765.
RefSeqiNP_037150.2. NM_013018.2.
UniGeneiRn.44409.

Genome annotation databases

EnsembliENSRNOT00000026391; ENSRNOP00000026392; ENSRNOG00000019433.
GeneIDi25531.
KEGGirno:25531.
UCSCiRGD:3528. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06889 mRNA. Translation: CAA30005.1 .
BC087580 mRNA. Translation: AAH87580.1 .
PIRi S01765.
RefSeqi NP_037150.2. NM_013018.2.
UniGenei Rn.44409.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZBD X-ray 2.60 A 19-217 [» ]
3RAB X-ray 2.00 A 18-186 [» ]
ProteinModelPortali P63012.
SMRi P63012. Positions 18-186.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247563. 3 interactions.
IntActi P63012. 4 interactions.
MINTi MINT-1506235.

PTM databases

PhosphoSitei P63012.

Proteomic databases

PaxDbi P63012.
PRIDEi P63012.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026391 ; ENSRNOP00000026392 ; ENSRNOG00000019433 .
GeneIDi 25531.
KEGGi rno:25531.
UCSCi RGD:3528. rat.

Organism-specific databases

CTDi 5864.
RGDi 3528. Rab3a.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118937.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi P63012.
KOi K07882.
OMAi QLTEQPA.
OrthoDBi EOG7JQBPC.
PhylomeDBi P63012.
TreeFami TF313199.

Enzyme and pathway databases

Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.
REACT_245524. Acetylcholine Neurotransmitter Release Cycle.
REACT_246747. Norepinephrine Neurotransmitter Release Cycle.
REACT_262380. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

EvolutionaryTracei P63012.
NextBioi 296339.
PROi P63012.

Gene expression databases

Genevestigatori P63012.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library."
    Touchot N., Chardin P., Tavitian A.
    Proc. Natl. Acad. Sci. U.S.A. 84:8210-8214(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Complete coding sequences of the ras related rab 3 and 4 cDNAs."
    Zahraoui A., Touchot N., Chardin P., Tavitian A.
    Nucleic Acids Res. 16:1204-1204(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A."
    Brondyk W.H., McKiernan C.J., Fortner K.A., Stabila P., Holz R.W., Macara I.G.
    Mol. Cell. Biol. 15:1137-1143(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB3IP, MUTAGENESIS OF THR-36; PHE-51; VAL-55 AND GLY-56.
  5. "The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins."
    Wang Y., Sugita S., Suedhof T.C.
    J. Biol. Chem. 275:20033-20044(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RIMS2.
  6. "Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A."
    Ostermeier C., Brunger A.T.
    Cell 96:363-374(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 19-217 IN COMPLEX WITH GTP AND RPH3A, INTERACTION WITH RPH3A.
  7. "Structural basis of activation and GTP hydrolysis in rab proteins."
    Dumas J.J., Zhu Z., Connolly J.L., Lambright D.G.
    Structure 7:413-423(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-186 IN COMPLEX WITH GTP ANALOG.

Entry informationi

Entry nameiRAB3A_RAT
AccessioniPrimary (citable) accession number: P63012
Secondary accession number(s): P05713
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3