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P63011 (RAB3A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-3A
Gene names
Name:Rab3a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal By similarity.

Subunit structure

Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3A and RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4, RIMS1 and RIMS2. Interacts with RAB3IP. Interacts with SGSM1 and SGSM3. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processExocytosis
Protein transport
Transport
   Cellular componentCell membrane
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Prenylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaxonogenesis

Inferred from mutant phenotype PubMed 11359932. Source: MGI

lung development

Inferred from mutant phenotype PubMed 11359932. Source: MGI

maintenance of presynaptic active zone structure

Inferred from mutant phenotype PubMed 11359932. Source: MGI

mitochondrion organization

Inferred from mutant phenotype PubMed 11359932. Source: MGI

neuromuscular synaptic transmission

Inferred from mutant phenotype PubMed 11359932. Source: MGI

post-embryonic development

Inferred from mutant phenotype PubMed 11359932. Source: MGI

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of exocytosis

Inferred from direct assay PubMed 12167638. Source: MGI

regulation of synaptic vesicle fusion to presynaptic membrane

Inferred from mutant phenotype PubMed 11359932. Source: MGI

respiratory system process

Inferred from mutant phenotype PubMed 11359932. Source: MGI

response to electrical stimulus

Inferred from mutant phenotype PubMed 11359932. Source: MGI

sensory perception of touch

Inferred from mutant phenotype PubMed 11359932. Source: MGI

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

synaptic vesicle exocytosis

Inferred from mutant phenotype PubMed 11359932. Source: MGI

synaptic vesicle maturation

Inferred from mutant phenotype PubMed 11359932. Source: MGI

   Cellular_componentacrosomal vesicle

Inferred from direct assay PubMed 21128978. Source: MGI

cytosol

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

protein complex

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle

Inferred from direct assay PubMed 12167638PubMed 12192047. Source: MGI

   Molecular_functionATPase activator activity

Inferred from electronic annotation. Source: Ensembl

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Rph3aP477082EBI-398393,EBI-398376
SYTL5Q8TDW52EBI-398393,EBI-2939487From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Ras-related protein Rab-3A
PRO_0000121078

Regions

Nucleotide binding29 – 368GTP By similarity
Nucleotide binding48 – 547GTP By similarity
Nucleotide binding77 – 815GTP By similarity
Nucleotide binding135 – 1384GTP By similarity
Motif51 – 599Effector region By similarity

Amino acid modifications

Modified residue2201Cysteine methyl ester By similarity
Lipidation2181S-geranylgeranyl cysteine By similarity
Lipidation2201S-geranylgeranyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P63011 [UniParc].

Last modified August 31, 2004. Version 1.
Checksum: 1A3E9F8C9D09EB40

FASTA22024,970
        10         20         30         40         50         60 
MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK 

        70         80         90        100        110        120 
VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWSTQI 

       130        140        150        160        170        180 
KTYSWDNAQV LLVGNKCDME DERVVSSERG RQLADHLGFE FFEASAKDNI NVKQTFERLV 

       190        200        210        220 
DVICEKMSES LDTADPAVTG AKQGPQLTDQ QAPPHQDCAC 

« Hide

References

« Hide 'large scale' references
[1]"Structure of the murine rab3A gene: correlation of genomic organization with antibody epitopes."
Baumert M., Fischer von Mollard G., Jahn R., Suedhof T.C.
Biochem. J. 293:157-163(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum and Visual cortex.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 13-35; 42-60; 73-83; 122-136; 152-167 AND 179-202, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[5]"cAMP-GEFII is a direct target of cAMP in regulated exocytosis."
Ozaki N., Shibasaki T., Kashima Y., Miki T., Takahashi K., Ueno H., Sunaga Y., Yano H., Matsuura Y., Iwanaga T., Takai Y., Seino S.
Nat. Cell Biol. 2:805-811(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RIMS2.
[6]"Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha-helix motifs."
Wang X., Hu B., Zimmermann B., Kilimann M.W.
J. Biol. Chem. 276:32480-32488(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RIMS1.
[7]"Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of cAMP-GEFII.Rim2.Piccolo complex in cAMP-dependent exocytosis."
Fujimoto K., Shibasaki T., Yokoi N., Kashima Y., Matsumoto M., Sasaki T., Tajima N., Iwanaga T., Seino S.
J. Biol. Chem. 277:50497-50502(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RIMS2.
[8]"Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2."
Fukuda M.
J. Biol. Chem. 278:15373-15380(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RIMS1; RIMS2; RPH3A AND RPH3AL.
[9]"Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells."
Fukuda M.
J. Biol. Chem. 278:15390-15396(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYTL4.
[10]"Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway."
Yang H., Sasaki T., Minoshima S., Shimizu N.
Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SGSM1 AND SGSM3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72966 Genomic DNA. Translation: CAA51470.1.
AK005362 mRNA. Translation: BAB23976.1.
AK158727 mRNA. Translation: BAE34630.1.
AK161975 mRNA. Translation: BAE36661.1.
BC053519 mRNA. Translation: AAH53519.1.
PIRS34070.
RefSeqNP_001159871.1. NM_001166399.2.
NP_033027.1. NM_009001.6.
XP_006509665.1. XM_006509602.1.
XP_006509666.1. XM_006509603.1.
UniGeneMm.5083.

3D structure databases

ProteinModelPortalP63011.
SMRP63011. Positions 18-186.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202544. 7 interactions.
DIPDIP-31051N.
IntActP63011. 11 interactions.
MINTMINT-85820.

PTM databases

PhosphoSiteP63011.

Proteomic databases

PaxDbP63011.
PRIDEP63011.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034301; ENSMUSP00000034301; ENSMUSG00000031840.
ENSMUST00000110090; ENSMUSP00000105717; ENSMUSG00000031840.
ENSMUST00000110092; ENSMUSP00000105719; ENSMUSG00000031840.
ENSMUST00000110093; ENSMUSP00000105720; ENSMUSG00000031840.
GeneID19339.
KEGGmmu:19339.
UCSCuc009mbi.2. mouse.

Organism-specific databases

CTD5864.
MGIMGI:97843. Rab3a.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP63011.
KOK07882.
OMAQLTEQPA.
OrthoDBEOG7JQBPC.
PhylomeDBP63011.
TreeFamTF313199.

Gene expression databases

ArrayExpressP63011.
BgeeP63011.
CleanExMM_RAB3A.
GenevestigatorP63011.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRAB3A. mouse.
NextBio296339.
PROP63011.
SOURCESearch...

Entry information

Entry nameRAB3A_MOUSE
AccessionPrimary (citable) accession number: P63011
Secondary accession number(s): P05713, Q3TSL4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot