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Protein

Amino-terminal enhancer of split

Gene

Aes

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Amino-terminal enhancer of split
Short name:
Amino enhancer of split
Alternative name(s):
Grg-5
Groucho-related protein 5
Protein ESP1
Short name:
R-esp1
Protein GRG
Gene namesi
Name:Aes
Synonyms:Esp1, Grg, Grg5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2731. Aes.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Amino-terminal enhancer of splitPRO_0000050836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei196 – 1961PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP63003.

PTM databases

iPTMnetiP63003.
PhosphoSiteiP63003.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with other family members. Binds TCF7 and the NF-kappa-B subunit RELA. Interacts with PHF12. Interacts (via Q domain) with SIX3. Interacts with SIX6 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248109. 1 interaction.
STRINGi10116.ENSRNOP00000059140.

Structurei

3D structure databases

ProteinModelPortaliP63003.
SMRiP63003. Positions 24-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 19732CCN domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 131131Gln-rich (Q domain)Add
BLAST
Compositional biasi132 – 16534Gly/Pro-rich (GP domain)Add
BLAST

Domaini

Lacks the C-terminal WD repeats.

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
HOGENOMiHOG000033830.
HOVERGENiHBG000205.
InParanoidiP63003.
OMAiMFPQARH.
OrthoDBiEOG7HQNC3.
PhylomeDBiP63003.

Family and domain databases

InterProiIPR005617. Groucho/TLE_N.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL
60 70 80 90 100
ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH
110 120 130 140 150
QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ
160 170 180 190
PPSLPAVSAG TGLLSLSALG SQTHLSKEDK NGHDGDTHQE DDGEKSD
Length:197
Mass (Da):22,000
Last modified:August 31, 2004 - v1
Checksum:i2C00233AA6921A9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14462 mRNA. Translation: AAC37639.1.
BC101855 mRNA. Translation: AAI01856.1.
PIRiA49555.
RefSeqiNP_062093.1. NM_019220.1.
UniGeneiRn.11495.

Genome annotation databases

GeneIDi29466.
KEGGirno:29466.
UCSCiRGD:2731. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14462 mRNA. Translation: AAC37639.1.
BC101855 mRNA. Translation: AAI01856.1.
PIRiA49555.
RefSeqiNP_062093.1. NM_019220.1.
UniGeneiRn.11495.

3D structure databases

ProteinModelPortaliP63003.
SMRiP63003. Positions 24-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248109. 1 interaction.
STRINGi10116.ENSRNOP00000059140.

PTM databases

iPTMnetiP63003.
PhosphoSiteiP63003.

Proteomic databases

PaxDbiP63003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29466.
KEGGirno:29466.
UCSCiRGD:2731. rat.

Organism-specific databases

CTDi166.
RGDi2731. Aes.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
HOGENOMiHOG000033830.
HOVERGENiHBG000205.
InParanoidiP63003.
OMAiMFPQARH.
OrthoDBiEOG7HQNC3.
PhylomeDBiP63003.

Miscellaneous databases

PROiP63003.

Family and domain databases

InterProiIPR005617. Groucho/TLE_N.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A rat homolog of the Drosophila enhancer of split (groucho) locus lacking WD-40 repeats."
    Schmidt C.J., Sladek T.E.
    J. Biol. Chem. 268:25681-25686(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hippocampus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAES_RAT
AccessioniPrimary (citable) accession number: P63003
Secondary accession number(s): Q06195, Q3MIF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.