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Protein

Amino-terminal enhancer of split

Gene

Aes

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.1 Publication

GO - Molecular functioni

  • transcription corepressor activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Amino-terminal enhancer of split
Short name:
Amino enhancer of split
Alternative name(s):
Grg-5
Groucho-related protein 5
Protein ESP1
Protein GRG
Gene namesi
Name:Aes
Synonyms:Esp1, Grg, Grg5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95806 Aes

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508351 – 197Amino-terminal enhancer of splitAdd BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63002
PaxDbiP63002
PeptideAtlasiP63002
PRIDEiP63002

PTM databases

iPTMnetiP63002
PhosphoSitePlusiP63002

Expressioni

Tissue specificityi

Ubiquitously expressed in developing embryos by midgestation, a wide expression is conserved in adult. In mouse, abundantly expressed in muscle, heart and brain.1 Publication

Developmental stagei

Expressed in the developing eye and forebrain of embryos.1 Publication

Gene expression databases

BgeeiENSMUSG00000054452
CleanExiMM_AES
ExpressionAtlasiP63002 baseline and differential
GenevisibleiP63002 MM

Interactioni

Subunit structurei

Homooligomer and heterooligomer with other family members. Binds TCF7 and the NF-kappa-B subunit RELA. Interacts with PHF12 (By similarity). Interacts (via Q domain) with SIX3. Interacts with SIX6.By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi200056, 8 interactors
CORUMiP63002
IntActiP63002, 7 interactors
MINTiP63002
STRINGi10090.ENSMUSP00000002518

Structurei

3D structure databases

ProteinModelPortaliP63002
SMRiP63002
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 197CCN domainAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 131Gln-rich (Q domain)Add BLAST131
Compositional biasi132 – 165Gly/Pro-rich (GP domain)Add BLAST34

Domaini

Lacks the C-terminal WD repeats.

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated

Phylogenomic databases

eggNOGiKOG0639 Eukaryota
ENOG410XPX3 LUCA
GeneTreeiENSGT00550000074465
HOGENOMiHOG000033830
HOVERGENiHBG000205
InParanoidiP63002
OMAiLANYSHG
OrthoDBiEOG091G0Q0N
PhylomeDBiP63002

Family and domain databases

InterProiView protein in InterPro
IPR005617 Groucho/TLE_N
PfamiView protein in Pfam
PF03920 TLE_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P63002-1) [UniParc]FASTAAdd to basket
Also known as: AES-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL
60 70 80 90 100
ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH
110 120 130 140 150
QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ
160 170 180 190
PPSLPAVSAG TGLLSLSALG SQTHLSKEDK NGHDGDTHQE DDGEKSD
Length:197
Mass (Da):22,000
Last modified:August 31, 2004 - v1
Checksum:i2C00233AA6921A9F
GO
Isoform 2 (identifier: P63002-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: AES-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MMFPQSRHSG → MGGAGRRSPSCPRRA

Show »
Length:202
Mass (Da):22,381
Checksum:i192775A7CD20430A
GO

Sequence cautioni

The sequence CAA51771 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0067711 – 10MMFPQSRHSG → MGGAGRRSPSCPRRA in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12140 mRNA Translation: AAA03023.1
X73359 mRNA Translation: CAA51769.1
X73361 mRNA Translation: CAA51771.1 Different initiation.
CCDSiCCDS24062.1
PIRiA56675
S35680
RefSeqiNP_034477.1, NM_010347.4 [P63002-1]
UniGeneiMm.180013

Genome annotation databases

EnsembliENSMUST00000002518; ENSMUSP00000002518; ENSMUSG00000054452 [P63002-1]
GeneIDi14797
KEGGimmu:14797
UCSCiuc007gim.2 mouse

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAES_MOUSE
AccessioniPrimary (citable) accession number: P63002
Secondary accession number(s): Q06195
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health