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Protein

Amino-terminal enhancer of split

Gene

Aes

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.1 Publication

GO - Molecular functioni

  • transcription corepressor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Amino-terminal enhancer of split
Short name:
Amino enhancer of split
Alternative name(s):
Grg-5
Groucho-related protein 5
Protein ESP1
Protein GRG
Gene namesi
Name:Aes
Synonyms:Esp1, Grg, Grg5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95806. Aes.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Amino-terminal enhancer of splitPRO_0000050835Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei196 – 1961PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63002.
PaxDbiP63002.
PRIDEiP63002.

PTM databases

iPTMnetiP63002.
PhosphoSiteiP63002.

Expressioni

Tissue specificityi

Ubiquitously expressed in developing embryos by midgestation, a wide expression is conserved in adult. In mouse, abundantly expressed in muscle, heart and brain.1 Publication

Developmental stagei

Expressed in the developing eye and forebrain of embryos.1 Publication

Gene expression databases

BgeeiP63002.
CleanExiMM_AES.
ExpressionAtlasiP63002. baseline and differential.
GenevisibleiP63002. MM.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with other family members. Binds TCF7 and the NF-kappa-B subunit RELA. Interacts with PHF12 (By similarity). Interacts (via Q domain) with SIX3. Interacts with SIX6.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Six3Q622335EBI-646888,EBI-2297327

Protein-protein interaction databases

BioGridi200056. 8 interactions.
IntActiP63002. 6 interactions.
MINTiMINT-225608.
STRINGi10090.ENSMUSP00000002518.

Structurei

3D structure databases

ProteinModelPortaliP63002.
SMRiP63002. Positions 24-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 19732CCN domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 131131Gln-rich (Q domain)Add
BLAST
Compositional biasi132 – 16534Gly/Pro-rich (GP domain)Add
BLAST

Domaini

Lacks the C-terminal WD repeats.

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
HOGENOMiHOG000033830.
HOVERGENiHBG000205.
InParanoidiP63002.
OMAiMFPQARH.
OrthoDBiEOG7HQNC3.
PhylomeDBiP63002.

Family and domain databases

InterProiIPR005617. Groucho/TLE_N.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P63002-1) [UniParc]FASTAAdd to basket

Also known as: AES-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL
60 70 80 90 100
ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH
110 120 130 140 150
QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ
160 170 180 190
PPSLPAVSAG TGLLSLSALG SQTHLSKEDK NGHDGDTHQE DDGEKSD
Length:197
Mass (Da):22,000
Last modified:August 31, 2004 - v1
Checksum:i2C00233AA6921A9F
GO
Isoform 2 (identifier: P63002-2) [UniParc] [UniParc]FASTAAdd to basket

Also known as: AES-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MMFPQSRHSG → MGGAGRRSPSCPRRA

Show »
Length:202
Mass (Da):22,381
Checksum:i192775A7CD20430A
GO

Sequence cautioni

The sequence CAA51771.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010MMFPQSRHSG → MGGAGRRSPSCPRRA in isoform 2. 1 PublicationVSP_006771

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12140 mRNA. Translation: AAA03023.1.
X73359 mRNA. Translation: CAA51769.1.
X73361 mRNA. Translation: CAA51771.1. Different initiation.
CCDSiCCDS24062.1.
PIRiA56675.
S35680.
RefSeqiNP_034477.1. NM_010347.4. [P63002-1]
UniGeneiMm.180013.

Genome annotation databases

EnsembliENSMUST00000002518; ENSMUSP00000002518; ENSMUSG00000054452. [P63002-1]
GeneIDi14797.
KEGGimmu:14797.
UCSCiuc007gim.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12140 mRNA. Translation: AAA03023.1.
X73359 mRNA. Translation: CAA51769.1.
X73361 mRNA. Translation: CAA51771.1. Different initiation.
CCDSiCCDS24062.1.
PIRiA56675.
S35680.
RefSeqiNP_034477.1. NM_010347.4. [P63002-1]
UniGeneiMm.180013.

3D structure databases

ProteinModelPortaliP63002.
SMRiP63002. Positions 24-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200056. 8 interactions.
IntActiP63002. 6 interactions.
MINTiMINT-225608.
STRINGi10090.ENSMUSP00000002518.

PTM databases

iPTMnetiP63002.
PhosphoSiteiP63002.

Proteomic databases

EPDiP63002.
PaxDbiP63002.
PRIDEiP63002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002518; ENSMUSP00000002518; ENSMUSG00000054452. [P63002-1]
GeneIDi14797.
KEGGimmu:14797.
UCSCiuc007gim.2. mouse.

Organism-specific databases

CTDi166.
MGIiMGI:95806. Aes.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
HOGENOMiHOG000033830.
HOVERGENiHBG000205.
InParanoidiP63002.
OMAiMFPQARH.
OrthoDBiEOG7HQNC3.
PhylomeDBiP63002.

Miscellaneous databases

ChiTaRSiAes. mouse.
PROiP63002.
SOURCEiSearch...

Gene expression databases

BgeeiP63002.
CleanExiMM_AES.
ExpressionAtlasiP63002. baseline and differential.
GenevisibleiP63002. MM.

Family and domain databases

InterProiIPR005617. Groucho/TLE_N.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and developmental expression of Grg, a mouse gene related to the groucho transcript of the Drosophila Enhancer of split complex."
    Mallo M., Franco del Amo F., Gridley T.
    Mech. Dev. 42:67-76(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein."
    Miyasaka H., Choudhury B.K., Hou E.W., Li S.S.-L.
    Eur. J. Biochem. 216:343-352(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
  3. "The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors."
    Roose J., Molenaar M., Peterson J., Hurenkamp J., Brantjes H., Moerer P., van de Wetering M., Destree O., Clevers H.
    Nature 395:608-612(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: OLIGOMERIZATION, INTERACTION WITH TCF7.
  4. "All Tcf HMG box transcription factors interact with Groucho-related co-repressors."
    Brantjes H., Roose J., van De Wetering M., Clevers H.
    Nucleic Acids Res. 29:1410-1419(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: OLIGOMERIZATION, INTERACTION WITH TCF7.
  5. "Six3-mediated auto repression and eye development requires its interaction with members of the Groucho-related family of co-repressors."
    Zhu C.C., Dyer M.A., Uchikawa M., Kondoh H., Lagutin O.V., Oliver G.
    Development 129:2835-2849(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH SIX3 AND SIX6.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiAES_MOUSE
AccessioniPrimary (citable) accession number: P63002
Secondary accession number(s): Q06195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.