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P62997

- TRA2B_RAT

UniProt

P62997 - TRA2B_RAT

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Protein

Transformer-2 protein homolog beta

Gene

Tra2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA (By similarity).By similarity

GO - Molecular functioni

  1. mRNA binding Source: UniProtKB
  2. nucleotide binding Source: InterPro
  3. RNA binding Source: RGD

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: UniProtKB
  2. positive regulation of mRNA splicing, via spliceosome Source: UniProtKB
  3. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  4. response to reactive oxygen species Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transformer-2 protein homolog beta
Short name:
TRA-2 beta
Short name:
TRA2-beta
Alternative name(s):
RA301
Splicing factor, arginine/serine-rich 10
Transformer-2 protein homolog B
Gene namesi
Name:Tra2b
Synonyms:Sfrs10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 11

Organism-specific databases

RGDi619886. Tra2b.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 288287Transformer-2 protein homolog betaPRO_0000081985Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei83 – 831PhosphoserineBy similarity
Modified residuei95 – 951PhosphoserineBy similarity
Modified residuei97 – 971PhosphoserineBy similarity
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei103 – 1031PhosphothreonineBy similarity
Modified residuei201 – 2011PhosphothreonineBy similarity
Modified residuei203 – 2031PhosphothreonineBy similarity
Modified residuei241 – 2411Dimethylated arginineBy similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP62997.
PRIDEiP62997.

PTM databases

PhosphoSiteiP62997.

Expressioni

Inductioni

Induced by reoxygenation following hypoxia and by exposure to silica. Repressed by interferon gamma, LPS and TPA.1 Publication

Gene expression databases

GenevestigatoriP62997.

Interactioni

Subunit structurei

Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RNPS1 and phosphorylated SFRS13A (By similarity). Interacts with RBMX and SAFB/SAFB1.By similarity2 Publications

Protein-protein interaction databases

IntActiP62997. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP62997.
SMRiP62997. Positions 106-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 19679RRMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 23038LinkerAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 11383Arg/Ser-rich (RS1 domain)Add
BLAST
Compositional biasi231 – 28757Arg/Ser-rich (RS2 domain)Add
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG297815.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62997.
KOiK12897.
OMAiEYYSRSY.
OrthoDBiEOG7SFHZZ.
PhylomeDBiP62997.
TreeFamiTF106265.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62997-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS
60 70 80 90 100
RSRSESRSRS RRSSRRHYTR SRSRSRSHRR SRSRSYSRDY RRRHSHSHSP
110 120 130 140 150
MSTRRRHVGN RANPDPNCCL GVFGLSLYTT ERDLREVFSK YGPIADVSIV
160 170 180 190 200
YDQQSRRSRG FAFVYFENVD DAKEAKERAN GMELDGRRIR VDFSITKRPH
210 220 230 240 250
TPTPGIYMGR PTYGSSRRRD YYDRGYDRGY DDRDYYSRSY RGGGGGGGGW
260 270 280
RAAQDRDQIY RRRSPSPYYS RGGYRSRSRS RSYSPRRY
Length:288
Mass (Da):33,666
Last modified:August 31, 2004 - v1
Checksum:i60B310C8BA443E28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49708 mRNA. Translation: BAA08556.1.
BC070948 mRNA. Translation: AAH70948.1.
RefSeqiNP_476460.1. NM_057119.1.
UniGeneiRn.8538.

Genome annotation databases

EnsembliENSRNOT00000002429; ENSRNOP00000002429; ENSRNOG00000001783.
GeneIDi117259.
KEGGirno:117259.
UCSCiRGD:619886. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49708 mRNA. Translation: BAA08556.1 .
BC070948 mRNA. Translation: AAH70948.1 .
RefSeqi NP_476460.1. NM_057119.1.
UniGenei Rn.8538.

3D structure databases

ProteinModelPortali P62997.
SMRi P62997. Positions 106-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P62997. 1 interaction.

PTM databases

PhosphoSitei P62997.

Proteomic databases

PaxDbi P62997.
PRIDEi P62997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000002429 ; ENSRNOP00000002429 ; ENSRNOG00000001783 .
GeneIDi 117259.
KEGGi rno:117259.
UCSCi RGD:619886. rat.

Organism-specific databases

CTDi 6434.
RGDi 619886. Tra2b.

Phylogenomic databases

eggNOGi NOG297815.
GeneTreei ENSGT00710000106295.
HOGENOMi HOG000276232.
HOVERGENi HBG104971.
InParanoidi P62997.
KOi K12897.
OMAi EYYSRSY.
OrthoDBi EOG7SFHZZ.
PhylomeDBi P62997.
TreeFami TF106265.

Miscellaneous databases

NextBioi 620130.
PROi P62997.

Gene expression databases

Genevestigatori P62997.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a novel RNA binding polypeptide (RA301) induced by hypoxia/reoxygenation."
    Matsuo N., Ogawa S., Imai Y., Takagi T., Tohyama M., Stern D., Wanaka A.
    J. Biol. Chem. 270:28216-28222(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Astrocyte.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. Cited for: INTERACTION WITH SAFB/SAFB1.
  4. "Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA."
    Heinrich B., Zhang Z., Raitskin O., Hiller M., Benderska N., Hartmann A.M., Bracco L., Elliott D., Ben-Ari S., Soreq H., Sperling J., Sperling R., Stamm S.
    J. Biol. Chem. 284:14303-14315(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RBMX.

Entry informationi

Entry nameiTRA2B_RAT
AccessioniPrimary (citable) accession number: P62997
Secondary accession number(s): O15449, Q15815, Q64283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3