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Protein

Transformer-2 protein homolog beta

Gene

Tra2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA (By similarity).By similarity

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly-purine tract binding Source: Ensembl
  • pre-mRNA binding Source: Ensembl
  • RNA binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Transformer-2 protein homolog beta
Short name:
TRA-2 beta
Short name:
TRA2-beta
Alternative name(s):
RA301
Splicing factor, arginine/serine-rich 10
Transformer-2 protein homolog B
Gene namesi
Name:Tra2b
Synonyms:Sfrs10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi619886. Tra2b.

Subcellular locationi

GO - Cellular componenti

  • nuclear inner membrane Source: Ensembl
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000819852 – 288Transformer-2 protein homolog betaAdd BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Modified residuei4PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei33PhosphothreonineCombined sources1
Modified residuei81PhosphoserineBy similarity1
Modified residuei83PhosphoserineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei95PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei99PhosphoserineCombined sources1
Modified residuei103PhosphothreonineBy similarity1
Modified residuei201PhosphothreonineCombined sources1
Modified residuei203PhosphothreonineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Modified residuei217Omega-N-methylarginineBy similarity1
Modified residuei237PhosphoserineBy similarity1
Modified residuei241Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei241Dimethylated arginine; alternateBy similarity1
Modified residuei241Omega-N-methylarginine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP62997.
PRIDEiP62997.

PTM databases

iPTMnetiP62997.
PhosphoSitePlusiP62997.
SwissPalmiP62997.

Expressioni

Inductioni

Induced by reoxygenation following hypoxia and by exposure to silica. Repressed by interferon gamma, LPS and TPA.1 Publication

Gene expression databases

BgeeiENSRNOG00000001783.
GenevisibleiP62997. RN.

Interactioni

Subunit structurei

Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RNPS1 and phosphorylated SFRS13A (By similarity). Interacts with RBMX and SAFB/SAFB1.By similarity2 Publications

Protein-protein interaction databases

IntActiP62997. 1 interactor.
STRINGi10116.ENSRNOP00000002429.

Structurei

3D structure databases

ProteinModelPortaliP62997.
SMRiP62997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini118 – 196RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni193 – 230LinkerAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 113Arg/Ser-rich (RS1 domain)Add BLAST83
Compositional biasi231 – 287Arg/Ser-rich (RS2 domain)Add BLAST57

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62997.
KOiK12897.
OMAiYSRDTDH.
OrthoDBiEOG091G0Y02.
PhylomeDBiP62997.
TreeFamiTF106265.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS
60 70 80 90 100
RSRSESRSRS RRSSRRHYTR SRSRSRSHRR SRSRSYSRDY RRRHSHSHSP
110 120 130 140 150
MSTRRRHVGN RANPDPNCCL GVFGLSLYTT ERDLREVFSK YGPIADVSIV
160 170 180 190 200
YDQQSRRSRG FAFVYFENVD DAKEAKERAN GMELDGRRIR VDFSITKRPH
210 220 230 240 250
TPTPGIYMGR PTYGSSRRRD YYDRGYDRGY DDRDYYSRSY RGGGGGGGGW
260 270 280
RAAQDRDQIY RRRSPSPYYS RGGYRSRSRS RSYSPRRY
Length:288
Mass (Da):33,666
Last modified:August 31, 2004 - v1
Checksum:i60B310C8BA443E28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49708 mRNA. Translation: BAA08556.1.
BC070948 mRNA. Translation: AAH70948.1.
RefSeqiNP_476460.1. NM_057119.1.
UniGeneiRn.8538.

Genome annotation databases

EnsembliENSRNOT00000002429; ENSRNOP00000002429; ENSRNOG00000001783.
GeneIDi117259.
KEGGirno:117259.
UCSCiRGD:619886. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49708 mRNA. Translation: BAA08556.1.
BC070948 mRNA. Translation: AAH70948.1.
RefSeqiNP_476460.1. NM_057119.1.
UniGeneiRn.8538.

3D structure databases

ProteinModelPortaliP62997.
SMRiP62997.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62997. 1 interactor.
STRINGi10116.ENSRNOP00000002429.

PTM databases

iPTMnetiP62997.
PhosphoSitePlusiP62997.
SwissPalmiP62997.

Proteomic databases

PaxDbiP62997.
PRIDEiP62997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002429; ENSRNOP00000002429; ENSRNOG00000001783.
GeneIDi117259.
KEGGirno:117259.
UCSCiRGD:619886. rat.

Organism-specific databases

CTDi6434.
RGDi619886. Tra2b.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62997.
KOiK12897.
OMAiYSRDTDH.
OrthoDBiEOG091G0Y02.
PhylomeDBiP62997.
TreeFamiTF106265.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiP62997.

Gene expression databases

BgeeiENSRNOG00000001783.
GenevisibleiP62997. RN.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRA2B_RAT
AccessioniPrimary (citable) accession number: P62997
Secondary accession number(s): O15449, Q15815, Q64283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.