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P62996 (TRA2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transformer-2 protein homolog beta

Short name=TRA-2 beta
Short name=TRA2-beta
Alternative name(s):
Silica-induced gene 41 protein
Short name=SIG-41
Splicing factor, arginine/serine-rich 10
Transformer-2 protein homolog B
Gene names
Name:Tra2b
Synonyms:Sfrs10, Silg41
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA By similarity.

Subunit structure

Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RNPS1, SAFB/SAFB1, RBMX and phosphorylated SFRS13A By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Widely expressed. Highly expressed in uterus and brain. Ref.2

Induction

Induced by reoxygenation following hypoxia and by exposure to silica. Repressed by interferon gamma, LPS and TPA. Ref.1

Sequence similarities

Belongs to the splicing factor SR family.

Contains 1 RRM (RNA recognition motif) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P62996-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P62996-2)

The sequence of this isoform differs from the canonical sequence as follows:
     23-38: AHGSGKSARHTPARSR → RHLTSFINEYLKLRNK
     39-288: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 288287Transformer-2 protein homolog beta
PRO_0000081984

Regions

Domain118 – 19679RRM
Region193 – 23038Linker
Compositional bias31 – 11383Arg/Ser-rich (RS1 domain)
Compositional bias231 – 28757Arg/Ser-rich (RS2 domain)

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity
Modified residue41Phosphoserine By similarity
Modified residue141Phosphoserine By similarity
Modified residue831Phosphoserine By similarity
Modified residue951Phosphoserine By similarity
Modified residue971Phosphoserine By similarity
Modified residue991Phosphoserine By similarity
Modified residue1031Phosphothreonine By similarity
Modified residue2011Phosphothreonine By similarity
Modified residue2031Phosphothreonine By similarity
Modified residue2411Dimethylated arginine By similarity

Natural variations

Alternative sequence23 – 3816AHGSG…PARSR → RHLTSFINEYLKLRNK in isoform 2.
VSP_011509
Alternative sequence39 – 288250Missing in isoform 2.
VSP_011510

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 31, 2004. Version 1.
Checksum: 60B310C8BA443E28

FASTA28833,666
        10         20         30         40         50         60 
MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS RSRSESRSRS 

        70         80         90        100        110        120 
RRSSRRHYTR SRSRSRSHRR SRSRSYSRDY RRRHSHSHSP MSTRRRHVGN RANPDPNCCL 

       130        140        150        160        170        180 
GVFGLSLYTT ERDLREVFSK YGPIADVSIV YDQQSRRSRG FAFVYFENVD DAKEAKERAN 

       190        200        210        220        230        240 
GMELDGRRIR VDFSITKRPH TPTPGIYMGR PTYGSSRRRD YYDRGYDRGY DDRDYYSRSY 

       250        260        270        280 
RGGGGGGGGW RAAQDRDQIY RRRSPSPYYS RGGYRSRSRS RSYSPRRY 

« Hide

Isoform 2 [UniParc].

Checksum: E499E3AFDD3D7BCA
Show »

FASTA384,392

References

« Hide 'large scale' references
[1]"Isolation of nine gene sequences induced by silica in murine macrophages."
Segade F., Claudio E., Wrobel K., Ramos S., Lazo P.S.
J. Immunol. 154:2384-2392(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION.
Tissue: Macrophage.
[2]"Molecular cloning of a mouse homologue for the Drosophila splicing regulator Tra2."
Segade F., Hurle B., Claudio E., Ramos S., Lazo P.S.
FEBS Lett. 387:152-156(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80232 mRNA. Translation: CAA56518.1.
AK049573 mRNA. Translation: BAC33819.1.
AK077418 mRNA. Translation: BAC36791.1.
AK080378 mRNA. Translation: BAC37898.1.
BC061177 mRNA. Translation: AAH61177.1.
CCDSCCDS37297.1. [P62996-1]
PIRS68798.
RefSeqNP_033212.1. NM_009186.4. [P62996-1]
UniGeneMm.210352.
Mm.389440.
Mm.442193.

3D structure databases

ProteinModelPortalP62996.
SMRP62996. Positions 106-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203252. 1 interaction.
IntActP62996. 2 interactions.
MINTMINT-4138402.

PTM databases

PhosphoSiteP62996.

Proteomic databases

PaxDbP62996.
PRIDEP62996.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000161286; ENSMUSP00000124846; ENSMUSG00000022858. [P62996-1]
ENSMUST00000162413; ENSMUSP00000123782; ENSMUSG00000022858.
GeneID20462.
KEGGmmu:20462.
UCSCuc007ysc.1. mouse. [P62996-1]

Organism-specific databases

CTD6434.
MGIMGI:106016. Tra2b.

Phylogenomic databases

eggNOGNOG297815.
GeneTreeENSGT00710000106295.
HOGENOMHOG000276232.
HOVERGENHBG104971.
InParanoidP62996.
KOK12897.
OMAEYYSRSY.
OrthoDBEOG7SFHZZ.
TreeFamTF106265.

Gene expression databases

ArrayExpressP62996.
BgeeP62996.
GenevestigatorP62996.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio24991.
PROP62996.
SOURCESearch...

Entry information

Entry nameTRA2B_MOUSE
AccessionPrimary (citable) accession number: P62996
Secondary accession number(s): O15449, Q15815, Q64283
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot