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Protein

Transformer-2 protein homolog beta

Gene

TRA2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.4 Publications

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • poly-purine tract binding Source: Ensembl
  • pre-mRNA binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136527-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Transformer-2 protein homolog beta
Short name:
TRA-2 beta
Short name:
TRA2-beta
Short name:
hTRA2-beta
Alternative name(s):
Splicing factor, arginine/serine-rich 10
Transformer-2 protein homolog B
Gene namesi
Name:TRA2B
Synonyms:SFRS10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10781. TRA2B.

Subcellular locationi

  • Nucleus 2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6434.
OpenTargetsiENSG00000136527.
PharmGKBiPA35697.

Polymorphism and mutation databases

BioMutaiTRA2B.
DMDMi51703330.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000819832 – 288Transformer-2 protein homolog betaAdd BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei4PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei29PhosphoserineBy similarity1
Modified residuei33PhosphothreonineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphoserineCombined sources1
Modified residuei87PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei99PhosphoserineCombined sources1
Modified residuei103PhosphothreonineCombined sources1
Modified residuei201PhosphothreonineCombined sources1
Modified residuei203PhosphothreonineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Modified residuei217Omega-N-methylarginineBy similarity1
Modified residuei237PhosphoserineCombined sources1
Modified residuei241Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei241Dimethylated arginine; alternateCombined sources1 Publication1
Modified residuei241Omega-N-methylarginine; alternateCombined sources1

Post-translational modificationi

Phosphorylated in the RS domains.1 Publication
Dimethylation at Arg-241 is probably asymmetric.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP62995.
MaxQBiP62995.
PaxDbiP62995.
PeptideAtlasiP62995.
PRIDEiP62995.

PTM databases

iPTMnetiP62995.
PhosphoSitePlusiP62995.
SwissPalmiP62995.

Expressioni

Tissue specificityi

Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.1 Publication

Gene expression databases

BgeeiENSG00000136527.
CleanExiHS_SFRS10.
ExpressionAtlasiP62995. baseline and differential.
GenevisibleiP62995. HS.

Organism-specific databases

HPAiCAB011692.
HPA063597.
HPA064972.

Interactioni

Subunit structurei

Interacts with SAFB/SAFB1 (By similarity). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RBMX, RNPS1 and phosphorylated SFRS13A.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RBMY1JQ154154EBI-725485,EBI-8642021
SRPK2P783623EBI-725485,EBI-593303
SRSF4Q081703EBI-725485,EBI-722621
YTHDC1Q96MU73EBI-725485,EBI-2849854

Protein-protein interaction databases

BioGridi112332. 84 interactors.
DIPiDIP-42278N.
IntActiP62995. 33 interactors.
MINTiMINT-5004415.
STRINGi9606.ENSP00000416959.

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi116 – 118Combined sources3
Beta strandi120 – 124Combined sources5
Helixi131 – 139Combined sources9
Beta strandi144 – 151Combined sources8
Beta strandi153 – 168Combined sources16
Helixi169 – 179Combined sources11
Beta strandi182 – 184Combined sources3
Beta strandi187 – 191Combined sources5
Beta strandi195 – 198Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQCNMR-A110-191[»]
2KXNNMR-B106-200[»]
2RRANMR-A109-201[»]
2RRBNMR-A111-201[»]
ProteinModelPortaliP62995.
SMRiP62995.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62995.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini118 – 196RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni193 – 230LinkerAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 113Arg/Ser-rich (RS1 domain)Add BLAST83
Compositional biasi231 – 287Arg/Ser-rich (RS2 domain)Add BLAST57

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQXD. Eukaryota.
ENOG4110CTH. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62995.
KOiK12897.
OMAiYSRDTDH.
OrthoDBiEOG091G0Y02.
PhylomeDBiP62995.
TreeFamiTF106265.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62995-1) [UniParc]FASTAAdd to basket
Also known as: HTRA2-beta1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS
60 70 80 90 100
RSRSESRSRS RRSSRRHYTR SRSRSRSHRR SRSRSYSRDY RRRHSHSHSP
110 120 130 140 150
MSTRRRHVGN RANPDPNCCL GVFGLSLYTT ERDLREVFSK YGPIADVSIV
160 170 180 190 200
YDQQSRRSRG FAFVYFENVD DAKEAKERAN GMELDGRRIR VDFSITKRPH
210 220 230 240 250
TPTPGIYMGR PTYGSSRRRD YYDRGYDRGY DDRDYYSRSY RGGGGGGGGW
260 270 280
RAAQDRDQIY RRRSPSPYYS RGGYRSRSRS RSYSPRRY
Length:288
Mass (Da):33,666
Last modified:August 31, 2004 - v1
Checksum:i60B310C8BA443E28
GO
Isoform 2 (identifier: P62995-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: HTRA2-beta2

The sequence of this isoform differs from the canonical sequence as follows:
     13-38: ESRSASRSGSAHGSGKSARHTPARSR → VNVEEGKCGSRHLTSFINEYLKLRNK
     39-288: Missing.

Show »
Length:38
Mass (Da):4,390
Checksum:i9E1E02297010CF61
GO
Isoform 3 (identifier: P62995-3) [UniParc] [UniParc]FASTAAdd to basket
Also known as: HTRA2-beta3

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:188
Mass (Da):21,935
Checksum:iAC9D4171E481BAF7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0058961 – 100Missing in isoform 3. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_00589813 – 38ESRSA…PARSR → VNVEEGKCGSRHLTSFINEY LKLRNK in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_00589939 – 288Missing in isoform 2. 1 PublicationAdd BLAST250

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61267 mRNA. Translation: AAC28242.1.
U87836 mRNA. Translation: AAB69763.1.
AF057159 Genomic DNA. Translation: AAD19277.1.
AF057159 Genomic DNA. Translation: AAD19278.1.
AF057159 Genomic DNA. Translation: AAD19279.1.
U68063 mRNA. Translation: AAB08701.1.
AK301118 mRNA. Translation: BAG62714.1.
CH471052 Genomic DNA. Translation: EAW78204.1.
CH471052 Genomic DNA. Translation: EAW78205.1.
CH471052 Genomic DNA. Translation: EAW78208.1.
BC000160 mRNA. Translation: AAH00160.1.
BC000451 mRNA. Translation: AAH00451.1.
BC005898 mRNA. Translation: AAH05898.1.
CCDSiCCDS33905.1.
CCDS58872.1. [P62995-3]
RefSeqiNP_001230808.1. NM_001243879.1. [P62995-3]
NP_004584.1. NM_004593.2. [P62995-1]
XP_005247760.1. XM_005247703.1. [P62995-3]
XP_011511374.1. XM_011513072.2. [P62995-3]
XP_016862522.1. XM_017007033.1. [P62995-3]
UniGeneiHs.533122.

Genome annotation databases

EnsembliENST00000342294; ENSP00000343857; ENSG00000136527. [P62995-2]
ENST00000382191; ENSP00000371626; ENSG00000136527. [P62995-3]
ENST00000453386; ENSP00000416959; ENSG00000136527. [P62995-1]
ENST00000456380; ENSP00000416887; ENSG00000136527. [P62995-2]
GeneIDi6434.
KEGGihsa:6434.
UCSCiuc003fpv.4. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61267 mRNA. Translation: AAC28242.1.
U87836 mRNA. Translation: AAB69763.1.
AF057159 Genomic DNA. Translation: AAD19277.1.
AF057159 Genomic DNA. Translation: AAD19278.1.
AF057159 Genomic DNA. Translation: AAD19279.1.
U68063 mRNA. Translation: AAB08701.1.
AK301118 mRNA. Translation: BAG62714.1.
CH471052 Genomic DNA. Translation: EAW78204.1.
CH471052 Genomic DNA. Translation: EAW78205.1.
CH471052 Genomic DNA. Translation: EAW78208.1.
BC000160 mRNA. Translation: AAH00160.1.
BC000451 mRNA. Translation: AAH00451.1.
BC005898 mRNA. Translation: AAH05898.1.
CCDSiCCDS33905.1.
CCDS58872.1. [P62995-3]
RefSeqiNP_001230808.1. NM_001243879.1. [P62995-3]
NP_004584.1. NM_004593.2. [P62995-1]
XP_005247760.1. XM_005247703.1. [P62995-3]
XP_011511374.1. XM_011513072.2. [P62995-3]
XP_016862522.1. XM_017007033.1. [P62995-3]
UniGeneiHs.533122.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQCNMR-A110-191[»]
2KXNNMR-B106-200[»]
2RRANMR-A109-201[»]
2RRBNMR-A111-201[»]
ProteinModelPortaliP62995.
SMRiP62995.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112332. 84 interactors.
DIPiDIP-42278N.
IntActiP62995. 33 interactors.
MINTiMINT-5004415.
STRINGi9606.ENSP00000416959.

PTM databases

iPTMnetiP62995.
PhosphoSitePlusiP62995.
SwissPalmiP62995.

Polymorphism and mutation databases

BioMutaiTRA2B.
DMDMi51703330.

Proteomic databases

EPDiP62995.
MaxQBiP62995.
PaxDbiP62995.
PeptideAtlasiP62995.
PRIDEiP62995.

Protocols and materials databases

DNASUi6434.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342294; ENSP00000343857; ENSG00000136527. [P62995-2]
ENST00000382191; ENSP00000371626; ENSG00000136527. [P62995-3]
ENST00000453386; ENSP00000416959; ENSG00000136527. [P62995-1]
ENST00000456380; ENSP00000416887; ENSG00000136527. [P62995-2]
GeneIDi6434.
KEGGihsa:6434.
UCSCiuc003fpv.4. human.

Organism-specific databases

CTDi6434.
DisGeNETi6434.
GeneCardsiTRA2B.
H-InvDBHIX0003928.
HGNCiHGNC:10781. TRA2B.
HPAiCAB011692.
HPA063597.
HPA064972.
MIMi602719. gene.
neXtProtiNX_P62995.
OpenTargetsiENSG00000136527.
PharmGKBiPA35697.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQXD. Eukaryota.
ENOG4110CTH. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62995.
KOiK12897.
OMAiYSRDTDH.
OrthoDBiEOG091G0Y02.
PhylomeDBiP62995.
TreeFamiTF106265.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136527-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiTRA2B. human.
EvolutionaryTraceiP62995.
GeneWikiiTRA2B.
GenomeRNAii6434.
PROiP62995.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136527.
CleanExiHS_SFRS10.
ExpressionAtlasiP62995. baseline and differential.
GenevisibleiP62995. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRA2B_HUMAN
AccessioniPrimary (citable) accession number: P62995
Secondary accession number(s): B4DVK2
, D3DNU3, O15449, Q15815, Q64283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.