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Protein

Transformer-2 protein homolog beta

Gene

TRA2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.4 Publications

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • cerebral cortex regionalization Source: Ensembl
  • mRNA splicing, via spliceosome Source: UniProtKB
  • positive regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing, via transesterification reactions Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Transformer-2 protein homolog beta
Short name:
TRA-2 beta
Short name:
TRA2-beta
Short name:
hTRA2-beta
Alternative name(s):
Splicing factor, arginine/serine-rich 10
Transformer-2 protein homolog B
Gene namesi
Name:TRA2B
Synonyms:SFRS10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10781. TRA2B.

Subcellular locationi

  • Nucleus 2 Publications

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35697.

Polymorphism and mutation databases

BioMutaiTRA2B.
DMDMi51703330.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 288287Transformer-2 protein homolog betaPRO_0000081983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei4 – 41PhosphoserineCombined sources
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei29 – 291PhosphoserineBy similarity
Modified residuei33 – 331PhosphothreonineBy similarity
Modified residuei81 – 811PhosphoserineBy similarity
Modified residuei83 – 831PhosphoserineCombined sources
Modified residuei85 – 851PhosphoserineCombined sources
Modified residuei87 – 871PhosphoserineCombined sources
Modified residuei95 – 951PhosphoserineCombined sources
Modified residuei97 – 971PhosphoserineCombined sources
Modified residuei99 – 991PhosphoserineCombined sources
Modified residuei103 – 1031PhosphothreonineCombined sources
Modified residuei201 – 2011PhosphothreonineCombined sources
Modified residuei203 – 2031PhosphothreonineCombined sources
Modified residuei215 – 2151PhosphoserineCombined sources
Modified residuei237 – 2371PhosphoserineCombined sources
Modified residuei241 – 2411Dimethylated arginineCombined sources1 Publication

Post-translational modificationi

Phosphorylated in the RS domains.1 Publication
Dimethylation at Arg-241 is probably asymmetric.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP62995.
MaxQBiP62995.
PaxDbiP62995.
PeptideAtlasiP62995.
PRIDEiP62995.

PTM databases

iPTMnetiP62995.
PhosphoSiteiP62995.
SwissPalmiP62995.

Expressioni

Tissue specificityi

Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.1 Publication

Gene expression databases

BgeeiENSG00000136527.
CleanExiHS_SFRS10.
ExpressionAtlasiP62995. baseline and differential.
GenevisibleiP62995. HS.

Organism-specific databases

HPAiCAB011692.
HPA063597.
HPA064972.

Interactioni

Subunit structurei

Interacts with SAFB/SAFB1 (By similarity). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RBMX, RNPS1 and phosphorylated SFRS13A.By similarity7 Publications

Protein-protein interaction databases

BioGridi112332. 84 interactions.
DIPiDIP-42278N.
IntActiP62995. 26 interactions.
MINTiMINT-5004415.
STRINGi9606.ENSP00000416959.

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi116 – 1183Combined sources
Beta strandi120 – 1245Combined sources
Helixi131 – 1399Combined sources
Beta strandi144 – 1518Combined sources
Beta strandi153 – 16816Combined sources
Helixi169 – 17911Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi187 – 1915Combined sources
Beta strandi195 – 1984Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CQCNMR-A110-191[»]
2KXNNMR-B106-200[»]
2RRANMR-A109-201[»]
2RRBNMR-A111-201[»]
ProteinModelPortaliP62995.
SMRiP62995. Positions 106-229.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62995.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 19679RRMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 23038LinkerAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 11383Arg/Ser-rich (RS1 domain)Add
BLAST
Compositional biasi231 – 28757Arg/Ser-rich (RS2 domain)Add
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQXD. Eukaryota.
ENOG4110CTH. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62995.
KOiK12897.
OMAiYSRDTDH.
OrthoDBiEOG091G0Y02.
PhylomeDBiP62995.
TreeFamiTF106265.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62995-1) [UniParc]FASTAAdd to basket
Also known as: HTRA2-beta1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS
60 70 80 90 100
RSRSESRSRS RRSSRRHYTR SRSRSRSHRR SRSRSYSRDY RRRHSHSHSP
110 120 130 140 150
MSTRRRHVGN RANPDPNCCL GVFGLSLYTT ERDLREVFSK YGPIADVSIV
160 170 180 190 200
YDQQSRRSRG FAFVYFENVD DAKEAKERAN GMELDGRRIR VDFSITKRPH
210 220 230 240 250
TPTPGIYMGR PTYGSSRRRD YYDRGYDRGY DDRDYYSRSY RGGGGGGGGW
260 270 280
RAAQDRDQIY RRRSPSPYYS RGGYRSRSRS RSYSPRRY
Length:288
Mass (Da):33,666
Last modified:August 31, 2004 - v1
Checksum:i60B310C8BA443E28
GO
Isoform 2 (identifier: P62995-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: HTRA2-beta2

The sequence of this isoform differs from the canonical sequence as follows:
     13-38: ESRSASRSGSAHGSGKSARHTPARSR → VNVEEGKCGSRHLTSFINEYLKLRNK
     39-288: Missing.

Show »
Length:38
Mass (Da):4,390
Checksum:i9E1E02297010CF61
GO
Isoform 3 (identifier: P62995-3) [UniParc] [UniParc]FASTAAdd to basket
Also known as: HTRA2-beta3

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:188
Mass (Da):21,935
Checksum:iAC9D4171E481BAF7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 100100Missing in isoform 3. 1 PublicationVSP_005896Add
BLAST
Alternative sequencei13 – 3826ESRSA…PARSR → VNVEEGKCGSRHLTSFINEY LKLRNK in isoform 2. 1 PublicationVSP_005898Add
BLAST
Alternative sequencei39 – 288250Missing in isoform 2. 1 PublicationVSP_005899Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61267 mRNA. Translation: AAC28242.1.
U87836 mRNA. Translation: AAB69763.1.
AF057159 Genomic DNA. Translation: AAD19277.1.
AF057159 Genomic DNA. Translation: AAD19278.1.
AF057159 Genomic DNA. Translation: AAD19279.1.
U68063 mRNA. Translation: AAB08701.1.
AK301118 mRNA. Translation: BAG62714.1.
CH471052 Genomic DNA. Translation: EAW78204.1.
CH471052 Genomic DNA. Translation: EAW78205.1.
CH471052 Genomic DNA. Translation: EAW78208.1.
BC000160 mRNA. Translation: AAH00160.1.
BC000451 mRNA. Translation: AAH00451.1.
BC005898 mRNA. Translation: AAH05898.1.
CCDSiCCDS33905.1.
CCDS58872.1. [P62995-3]
RefSeqiNP_001230808.1. NM_001243879.1. [P62995-3]
NP_004584.1. NM_004593.2. [P62995-1]
XP_005247760.1. XM_005247703.1. [P62995-3]
XP_011511374.1. XM_011513072.2. [P62995-3]
UniGeneiHs.533122.

Genome annotation databases

EnsembliENST00000342294; ENSP00000343857; ENSG00000136527. [P62995-2]
ENST00000382191; ENSP00000371626; ENSG00000136527. [P62995-3]
ENST00000453386; ENSP00000416959; ENSG00000136527. [P62995-1]
ENST00000456380; ENSP00000416887; ENSG00000136527. [P62995-2]
GeneIDi6434.
KEGGihsa:6434.
UCSCiuc003fpv.4. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61267 mRNA. Translation: AAC28242.1.
U87836 mRNA. Translation: AAB69763.1.
AF057159 Genomic DNA. Translation: AAD19277.1.
AF057159 Genomic DNA. Translation: AAD19278.1.
AF057159 Genomic DNA. Translation: AAD19279.1.
U68063 mRNA. Translation: AAB08701.1.
AK301118 mRNA. Translation: BAG62714.1.
CH471052 Genomic DNA. Translation: EAW78204.1.
CH471052 Genomic DNA. Translation: EAW78205.1.
CH471052 Genomic DNA. Translation: EAW78208.1.
BC000160 mRNA. Translation: AAH00160.1.
BC000451 mRNA. Translation: AAH00451.1.
BC005898 mRNA. Translation: AAH05898.1.
CCDSiCCDS33905.1.
CCDS58872.1. [P62995-3]
RefSeqiNP_001230808.1. NM_001243879.1. [P62995-3]
NP_004584.1. NM_004593.2. [P62995-1]
XP_005247760.1. XM_005247703.1. [P62995-3]
XP_011511374.1. XM_011513072.2. [P62995-3]
UniGeneiHs.533122.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CQCNMR-A110-191[»]
2KXNNMR-B106-200[»]
2RRANMR-A109-201[»]
2RRBNMR-A111-201[»]
ProteinModelPortaliP62995.
SMRiP62995. Positions 106-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112332. 84 interactions.
DIPiDIP-42278N.
IntActiP62995. 26 interactions.
MINTiMINT-5004415.
STRINGi9606.ENSP00000416959.

PTM databases

iPTMnetiP62995.
PhosphoSiteiP62995.
SwissPalmiP62995.

Polymorphism and mutation databases

BioMutaiTRA2B.
DMDMi51703330.

Proteomic databases

EPDiP62995.
MaxQBiP62995.
PaxDbiP62995.
PeptideAtlasiP62995.
PRIDEiP62995.

Protocols and materials databases

DNASUi6434.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342294; ENSP00000343857; ENSG00000136527. [P62995-2]
ENST00000382191; ENSP00000371626; ENSG00000136527. [P62995-3]
ENST00000453386; ENSP00000416959; ENSG00000136527. [P62995-1]
ENST00000456380; ENSP00000416887; ENSG00000136527. [P62995-2]
GeneIDi6434.
KEGGihsa:6434.
UCSCiuc003fpv.4. human.

Organism-specific databases

CTDi6434.
GeneCardsiTRA2B.
H-InvDBHIX0003928.
HGNCiHGNC:10781. TRA2B.
HPAiCAB011692.
HPA063597.
HPA064972.
MIMi602719. gene.
neXtProtiNX_P62995.
PharmGKBiPA35697.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQXD. Eukaryota.
ENOG4110CTH. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG104971.
InParanoidiP62995.
KOiK12897.
OMAiYSRDTDH.
OrthoDBiEOG091G0Y02.
PhylomeDBiP62995.
TreeFamiTF106265.

Enzyme and pathway databases

ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiTRA2B. human.
EvolutionaryTraceiP62995.
GeneWikiiTRA2B.
GenomeRNAii6434.
PROiP62995.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136527.
CleanExiHS_SFRS10.
ExpressionAtlasiP62995. baseline and differential.
GenevisibleiP62995. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRA2B_HUMAN
AccessioniPrimary (citable) accession number: P62995
Secondary accession number(s): B4DVK2
, D3DNU3, O15449, Q15815, Q64283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.