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Protein

Ubiquitin-40S ribosomal protein S27a

Gene

Rps27a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.1 Publication
40S Ribosomal protein S27a: Component of the 40S subunit of the ribosome.1 Publication

Miscellaneous

Ubiquitin is encoded by 4 different genes. Uba52 and Rps27a genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Activating enzyme1
Sitei68Essential for function1
Binding sitei72Activating enzyme1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 144C4-typeAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-MMU-400253. Circadian Clock.
R-MMU-508751. Circadian Clock.
R-MMU-5632684. Hedgehog 'on' state.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-40S ribosomal protein S27a
Alternative name(s):
Ubiquitin carboxyl extension protein 80
Cleaved into the following 2 chains:
Gene namesi
Name:Rps27a
Synonyms:Uba80, Ubcep1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1925544. Rps27a.

Subcellular locationi

Ubiquitin :

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003964791 – 76UbiquitinAdd BLAST76
ChainiPRO_000013766377 – 15640S ribosomal protein S27aAdd BLAST80

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei65Phosphoserine; by PINK1By similarity1
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation
Modified residuei104N6-acetyllysineBy similarity1
Modified residuei113N6-acetyllysineBy similarity1
Modified residuei152N6-acetyllysineCombined sources1

Post-translational modificationi

Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PARK2), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP62983.
PaxDbiP62983.
PeptideAtlasiP62983.
PRIDEiP62983.
TopDownProteomicsiP62983.

2D gel databases

REPRODUCTION-2DPAGEiP62991.

PTM databases

iPTMnetiP62983.
PhosphoSitePlusiP62983.
SwissPalmiP62983.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020460.
CleanExiMM_RPS27A.
ExpressionAtlasiP62983. baseline and differential.
GenevisibleiP62983. MM.

Interactioni

Subunit structurei

Ribosomal protein S27a is part of the 40S ribosomal subunit.By similarity

Protein-protein interaction databases

BioGridi219309. 14 interactors.
IntActiP62983. 4 interactors.
STRINGi10090.ENSMUSP00000099908.

Structurei

3D structure databases

ProteinModelPortaliP62983.
SMRiP62983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 99Lys-rich (highly basic)Add BLAST23
Compositional biasi78 – 107Lys-richAdd BLAST30

Sequence similaritiesi

In the N-terminal section; belongs to the ubiquitin family.Curated
In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 144C4-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0004. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00860000133816.
HOVERGENiHBG079153.
InParanoidiP62983.
KOiK02977.
OMAiMSILKYY.
OrthoDBiEOG091G178I.
PhylomeDBiP62983.
TreeFamiTF300036.

Family and domain databases

InterProiView protein in InterPro
IPR002906. Ribosomal_S27a.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF01599. Ribosomal_S27. 1 hit.
PF00240. ubiquitin. 1 hit.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM01402. Ribosomal_S27. 1 hit.
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF57829. SSF57829. 1 hit.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPKK NKHKRKKVKL
110 120 130 140 150
AVLKYYKVDE NGKISRLRRE CPSDECGAGV FMGSHFDRHY CGKCCLTYCF

NKPEDK
Length:156
Mass (Da):17,951
Last modified:August 10, 2010 - v2
Checksum:iC11DC63DF3A904F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018706 mRNA. Translation: BAB31357.1.
BC002108 mRNA. Translation: AAH02108.1.
CCDSiCCDS24497.1.
RefSeqiNP_001029037.1. NM_001033865.1.
NP_077239.1. NM_024277.2.
UniGeneiMm.180003.
Mm.458088.

Genome annotation databases

EnsembliENSMUST00000102844; ENSMUSP00000099908; ENSMUSG00000020460.
ENSMUST00000102845; ENSMUSP00000099909; ENSMUSG00000020460.
GeneIDi78294.
KEGGimmu:78294.
UCSCiuc007ihg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018706 mRNA. Translation: BAB31357.1.
BC002108 mRNA. Translation: AAH02108.1.
CCDSiCCDS24497.1.
RefSeqiNP_001029037.1. NM_001033865.1.
NP_077239.1. NM_024277.2.
UniGeneiMm.180003.
Mm.458088.

3D structure databases

ProteinModelPortaliP62983.
SMRiP62983.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219309. 14 interactors.
IntActiP62983. 4 interactors.
STRINGi10090.ENSMUSP00000099908.

PTM databases

iPTMnetiP62983.
PhosphoSitePlusiP62983.
SwissPalmiP62983.

2D gel databases

REPRODUCTION-2DPAGEiP62991.

Proteomic databases

EPDiP62983.
PaxDbiP62983.
PeptideAtlasiP62983.
PRIDEiP62983.
TopDownProteomicsiP62983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102844; ENSMUSP00000099908; ENSMUSG00000020460.
ENSMUST00000102845; ENSMUSP00000099909; ENSMUSG00000020460.
GeneIDi78294.
KEGGimmu:78294.
UCSCiuc007ihg.1. mouse.

Organism-specific databases

CTDi6233.
MGIiMGI:1925544. Rps27a.

Phylogenomic databases

eggNOGiKOG0004. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00860000133816.
HOVERGENiHBG079153.
InParanoidiP62983.
KOiK02977.
OMAiMSILKYY.
OrthoDBiEOG091G178I.
PhylomeDBiP62983.
TreeFamiTF300036.

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-MMU-400253. Circadian Clock.
R-MMU-508751. Circadian Clock.
R-MMU-5632684. Hedgehog 'on' state.

Miscellaneous databases

PROiPR:P62983.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020460.
CleanExiMM_RPS27A.
ExpressionAtlasiP62983. baseline and differential.
GenevisibleiP62983. MM.

Family and domain databases

InterProiView protein in InterPro
IPR002906. Ribosomal_S27a.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF01599. Ribosomal_S27. 1 hit.
PF00240. ubiquitin. 1 hit.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM01402. Ribosomal_S27. 1 hit.
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF57829. SSF57829. 1 hit.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRS27A_MOUSE
AccessioniPrimary (citable) accession number: P62983
Secondary accession number(s): P02248
, P02249, P02250, P49664, P62991, Q29120, Q62317, Q64223, Q8VCH1, Q91887, Q91888, Q9CXY4, Q9CZM0, Q9D1R5, Q9D2W3, Q9D8D9, Q9ET23, Q9ET24, Q9Z0H9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 10, 2010
Last modified: April 12, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.