P62975 (UBIQ_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ubiquitin
Organism	Oryctolagus cuniculus (Rabbit) [Complete proteome]
Taxonomic identifier	9986 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Miscellaneous	Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species. In some species there is a final amino-acid after the last repeat. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Sequence similarities	Belongs to the ubiquitin family. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	Ubiquitin		PRO_0000114805
Regions
	Domain	1 – 76	76	Ubiquitin-like
Sites
	Binding site	54	1	Activating enzyme
	Binding site	72	1	Activating enzyme
	Site	68	1	Essential for function
Amino acid modifications
	Modified residue	6	1	N6-acetyllysine By similarity
	Modified residue	48	1	N6-acetyllysine By similarity
	Modified residue	57	1	Phosphoserine By similarity
	Modified residue	65	1	Phosphoserine By similarity
	Cross-link	6		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	11		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	27		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	29		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	33		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	48		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
	Cross-link	63		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	76		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Sequences

	Sequence		Length	Mass (Da)
	P62975 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: C42A35397FFD9B52	FASTA	76	8,565
	10 20 30 40 50 60 MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN 70 IQKESTLHLV LRLRGG

References

[1]	"Structural characterization of rabbit brain ubiquitin." Wajih N., Siddiqi A.R., Kaiser R., Persson B., Zaidi Z.H., Joernvall H. Protein Seq. Data Anal. 5:31-32(1992) [PubMed: 1337207] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	S28203.
3D structure databases
ProteinModelPortal	P62975.
SMR	P62975. Positions 1-76.
ModBase	Search...
Protein-protein interaction databases
STRING	P62975.
Proteomic databases
PRIDE	P62975.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	maNOG18772.
GeneTree	ENSGT00550000074658. ENSGT00550000074763.
OrthoDB	EOG4PZJ82. EOG4WDDB6.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019956. Ubiquitin_subgr. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
PRINTS	PR00348. UBIQUITIN.
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIQ_RABIT

Accession

Primary (citable) accession number: P62975
Secondary accession number(s): P02248 , P02249, P02250, Q29120, Q91887, Q91888

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program