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P62963 (PROF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Profilin-1
Alternative name(s):
Profilin I
Gene names
Name:Pfn1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length140 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR By similarity.

Subunit structure

Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 By similarity. Interacts with HTT By similarity.

Subcellular location

Cytoplasmcytoskeleton.

Post-translational modification

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation By similarity.

Sequence similarities

Belongs to the profilin family.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   LigandActin-binding
   PTMAcetylation
Isopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactin polymerization or depolymerization

Traceable author statement Ref.1. Source: RGD

cellular response to growth factor stimulus

Inferred from expression pattern PubMed 10703666. Source: RGD

neural tube closure

Inferred from electronic annotation. Source: Ensembl

positive regulation of DNA metabolic process

Inferred from direct assay PubMed 10966486. Source: RGD

positive regulation of stress fiber assembly

Inferred from mutant phenotype PubMed 12740026. Source: RGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10966486. Source: RGD

positive regulation of viral transcription

Inferred from mutant phenotype PubMed 12740026. Source: RGD

response to organic substance

Inferred from expression pattern PubMed 8297366. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12967995. Source: RGD

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuron projection

Inferred from direct assay PubMed 8106880. Source: RGD

nucleus

Inferred from electronic annotation. Source: Ensembl

synapse

Inferred from direct assay PubMed 12967995. Source: RGD

   Molecular_functionactin binding

Traceable author statement Ref.1. Source: RGD

phosphatidylinositol-4,5-bisphosphate binding

Inferred from direct assay PubMed 16741017. Source: RGD

receptor binding

Inferred from direct assay PubMed 10966486. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 140139Profilin-1
PRO_0000199573

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1081N6-acetyllysine By similarity
Modified residue1291Phosphotyrosine By similarity
Modified residue1381Phosphoserine; by ROCK1 By similarity
Cross-link54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
P62963 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 1DC8B60D545FF70E

FASTA14014,957
        10         20         30         40         50         60 
MAGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV LVGKDRSSFF 

        70         80         90        100        110        120 
VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN VTVTMTAKTL VLLMGKEGVH 

       130        140 
GGLINKKCYE MASHLRRSQY 

« Hide

References

« Hide 'large scale' references
[1]"Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis."
Tamura M., Tanaka H., Hirano T., Ueta Y., Higashi K., Hirano H.
Biochem. Biophys. Res. Commun. 222:683-687(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[3]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 39-54; 57-70 AND 76-89, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X96967 mRNA. Translation: CAA65655.1.
BC062405 mRNA. Translation: AAH62405.1.
PIRPC4172.
RefSeqNP_071956.2. NM_022511.2.
UniGeneRn.1152.

3D structure databases

ProteinModelPortalP62963.
SMRP62963. Positions 2-140.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249018. 4 interactions.
IntActP62963. 2 interactions.
MINTMINT-1868721.

PTM databases

PhosphoSiteP62963.

Proteomic databases

PaxDbP62963.
PRIDEP62963.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005370; ENSRNOP00000005370; ENSRNOG00000003975.
GeneID64303.
KEGGrno:64303.
UCSCRGD:621825. rat.

Organism-specific databases

CTD5216.
RGD621825. Pfn1.

Phylogenomic databases

eggNOGNOG269129.
GeneTreeENSGT00390000010143.
HOGENOMHOG000171592.
HOVERGENHBG053683.
InParanoidP62963.
KOK05759.
OMAHLRRAQY.
OrthoDBEOG7JMGGT.
PhylomeDBP62963.
TreeFamTF331744.

Gene expression databases

GenevestigatorP62963.

Family and domain databases

InterProIPR005454. Profilin_chordates.
IPR027310. Profilin_CS.
IPR005455. Profilin_eukaryotes/bac.
[Graphical view]
PfamPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSPR01639. PROFILINMAML.
SMARTSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMSSF55770. SSF55770. 1 hit.
PROSITEPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294634.
PROP62963.

Entry information

Entry namePROF1_RAT
AccessionPrimary (citable) accession number: P62963
Secondary accession number(s): P10924
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families