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P62963

- PROF1_RAT

UniProt

P62963 - PROF1_RAT

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Protein

Profilin-1

Gene

Pfn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity).By similarity

GO - Molecular functioni

  1. actin binding Source: RGD
  2. phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  3. receptor binding Source: RGD

GO - Biological processi

  1. actin polymerization or depolymerization Source: RGD
  2. cellular response to growth factor stimulus Source: RGD
  3. positive regulation of DNA metabolic process Source: RGD
  4. positive regulation of stress fiber assembly Source: RGD
  5. positive regulation of transcription from RNA polymerase II promoter Source: RGD
  6. positive regulation of viral transcription Source: RGD
  7. response to organic substance Source: RGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_198389. PCP/CE pathway.
REACT_239909. Signaling by Robo receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin-1
Alternative name(s):
Profilin I
Gene namesi
Name:Pfn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi621825. Pfn1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. cytoskeleton Source: UniProtKB-KW
  3. neuron projection Source: RGD
  4. synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 140139Profilin-1PRO_0000199573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki54 – 54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei108 – 1081N6-acetyllysineBy similarity
Modified residuei129 – 1291PhosphotyrosineBy similarity
Modified residuei138 – 1381Phosphoserine; by ROCK1By similarity

Post-translational modificationi

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62963.
PRIDEiP62963.

PTM databases

PhosphoSiteiP62963.

Expressioni

Gene expression databases

GenevestigatoriP62963.

Interactioni

Subunit structurei

Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 (By similarity). Interacts with HTT (By similarity).By similarity

Protein-protein interaction databases

BioGridi249018. 4 interactions.
IntActiP62963. 2 interactions.
MINTiMINT-1868721.

Structurei

3D structure databases

ProteinModelPortaliP62963.
SMRiP62963. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the profilin family.Curated

Phylogenomic databases

eggNOGiNOG269129.
GeneTreeiENSGT00390000010143.
HOGENOMiHOG000171592.
HOVERGENiHBG053683.
InParanoidiP62963.
KOiK05759.
OMAiHLRRAQY.
OrthoDBiEOG7JMGGT.
PhylomeDBiP62963.
TreeFamiTF331744.

Family and domain databases

InterProiIPR005455. PFN.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
[Graphical view]
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR01639. PROFILINMAML.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62963-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV
60 70 80 90 100
LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN
110 120 130 140
VTVTMTAKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
Length:140
Mass (Da):14,957
Last modified:January 23, 2007 - v2
Checksum:i1DC8B60D545FF70E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96967 mRNA. Translation: CAA65655.1.
BC062405 mRNA. Translation: AAH62405.1.
PIRiPC4172.
RefSeqiNP_071956.2. NM_022511.2.
UniGeneiRn.1152.

Genome annotation databases

EnsembliENSRNOT00000005370; ENSRNOP00000005370; ENSRNOG00000003975.
GeneIDi64303.
KEGGirno:64303.
UCSCiRGD:621825. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96967 mRNA. Translation: CAA65655.1 .
BC062405 mRNA. Translation: AAH62405.1 .
PIRi PC4172.
RefSeqi NP_071956.2. NM_022511.2.
UniGenei Rn.1152.

3D structure databases

ProteinModelPortali P62963.
SMRi P62963. Positions 2-140.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249018. 4 interactions.
IntActi P62963. 2 interactions.
MINTi MINT-1868721.

PTM databases

PhosphoSitei P62963.

Proteomic databases

PaxDbi P62963.
PRIDEi P62963.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000005370 ; ENSRNOP00000005370 ; ENSRNOG00000003975 .
GeneIDi 64303.
KEGGi rno:64303.
UCSCi RGD:621825. rat.

Organism-specific databases

CTDi 5216.
RGDi 621825. Pfn1.

Phylogenomic databases

eggNOGi NOG269129.
GeneTreei ENSGT00390000010143.
HOGENOMi HOG000171592.
HOVERGENi HBG053683.
InParanoidi P62963.
KOi K05759.
OMAi HLRRAQY.
OrthoDBi EOG7JMGGT.
PhylomeDBi P62963.
TreeFami TF331744.

Enzyme and pathway databases

Reactomei REACT_198389. PCP/CE pathway.
REACT_239909. Signaling by Robo receptor.

Miscellaneous databases

NextBioi 294634.
PROi P62963.

Gene expression databases

Genevestigatori P62963.

Family and domain databases

InterProi IPR005455. PFN.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
[Graphical view ]
Pfami PF00235. Profilin. 1 hit.
[Graphical view ]
PRINTSi PR01639. PROFILINMAML.
SMARTi SM00392. PROF. 1 hit.
[Graphical view ]
SUPFAMi SSF55770. SSF55770. 1 hit.
PROSITEi PS00414. PROFILIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis."
    Tamura M., Tanaka H., Hirano T., Ueta Y., Higashi K., Hirano H.
    Biochem. Biophys. Res. Commun. 222:683-687(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 39-54; 57-70 AND 76-89, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiPROF1_RAT
AccessioniPrimary (citable) accession number: P62963
Secondary accession number(s): P10924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3