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P62963

- PROF1_RAT

UniProt

P62963 - PROF1_RAT

Protein

Profilin-1

Gene

Pfn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR By similarity.By similarity

    GO - Molecular functioni

    1. actin binding Source: RGD
    2. phosphatidylinositol-4,5-bisphosphate binding Source: RGD
    3. protein binding Source: RGD
    4. receptor binding Source: RGD

    GO - Biological processi

    1. actin polymerization or depolymerization Source: RGD
    2. cellular response to growth factor stimulus Source: RGD
    3. neural tube closure Source: Ensembl
    4. positive regulation of DNA metabolic process Source: RGD
    5. positive regulation of stress fiber assembly Source: RGD
    6. positive regulation of transcription from RNA polymerase II promoter Source: RGD
    7. positive regulation of viral transcription Source: RGD
    8. response to organic substance Source: RGD

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_198389. PCP/CE pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Profilin-1
    Alternative name(s):
    Profilin I
    Gene namesi
    Name:Pfn1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi621825. Pfn1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. cytoskeleton Source: UniProtKB-SubCell
    3. neuron projection Source: RGD
    4. nucleus Source: Ensembl
    5. synapse Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 140139Profilin-1PRO_0000199573Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Cross-linki54 – 54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei108 – 1081N6-acetyllysineBy similarity
    Modified residuei129 – 1291PhosphotyrosineBy similarity
    Modified residuei138 – 1381Phosphoserine; by ROCK1By similarity

    Post-translational modificationi

    Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP62963.
    PRIDEiP62963.

    PTM databases

    PhosphoSiteiP62963.

    Expressioni

    Gene expression databases

    GenevestigatoriP62963.

    Interactioni

    Subunit structurei

    Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 By similarity. Interacts with HTT By similarity.By similarity

    Protein-protein interaction databases

    BioGridi249018. 4 interactions.
    IntActiP62963. 2 interactions.
    MINTiMINT-1868721.

    Structurei

    3D structure databases

    ProteinModelPortaliP62963.
    SMRiP62963. Positions 2-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the profilin family.Curated

    Phylogenomic databases

    eggNOGiNOG269129.
    GeneTreeiENSGT00390000010143.
    HOGENOMiHOG000171592.
    HOVERGENiHBG053683.
    InParanoidiP62963.
    KOiK05759.
    OMAiHLRRAQY.
    OrthoDBiEOG7JMGGT.
    PhylomeDBiP62963.
    TreeFamiTF331744.

    Family and domain databases

    InterProiIPR005454. Profilin_chordates.
    IPR027310. Profilin_CS.
    IPR005455. Profilin_eukaryotes/bac.
    [Graphical view]
    PfamiPF00235. Profilin. 1 hit.
    [Graphical view]
    PRINTSiPR01639. PROFILINMAML.
    SMARTiSM00392. PROF. 1 hit.
    [Graphical view]
    SUPFAMiSSF55770. SSF55770. 1 hit.
    PROSITEiPS00414. PROFILIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62963-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV    50
    LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN 100
    VTVTMTAKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY 140
    Length:140
    Mass (Da):14,957
    Last modified:January 23, 2007 - v2
    Checksum:i1DC8B60D545FF70E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96967 mRNA. Translation: CAA65655.1.
    BC062405 mRNA. Translation: AAH62405.1.
    PIRiPC4172.
    RefSeqiNP_071956.2. NM_022511.2.
    UniGeneiRn.1152.

    Genome annotation databases

    EnsembliENSRNOT00000005370; ENSRNOP00000005370; ENSRNOG00000003975.
    GeneIDi64303.
    KEGGirno:64303.
    UCSCiRGD:621825. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96967 mRNA. Translation: CAA65655.1 .
    BC062405 mRNA. Translation: AAH62405.1 .
    PIRi PC4172.
    RefSeqi NP_071956.2. NM_022511.2.
    UniGenei Rn.1152.

    3D structure databases

    ProteinModelPortali P62963.
    SMRi P62963. Positions 2-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249018. 4 interactions.
    IntActi P62963. 2 interactions.
    MINTi MINT-1868721.

    PTM databases

    PhosphoSitei P62963.

    Proteomic databases

    PaxDbi P62963.
    PRIDEi P62963.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005370 ; ENSRNOP00000005370 ; ENSRNOG00000003975 .
    GeneIDi 64303.
    KEGGi rno:64303.
    UCSCi RGD:621825. rat.

    Organism-specific databases

    CTDi 5216.
    RGDi 621825. Pfn1.

    Phylogenomic databases

    eggNOGi NOG269129.
    GeneTreei ENSGT00390000010143.
    HOGENOMi HOG000171592.
    HOVERGENi HBG053683.
    InParanoidi P62963.
    KOi K05759.
    OMAi HLRRAQY.
    OrthoDBi EOG7JMGGT.
    PhylomeDBi P62963.
    TreeFami TF331744.

    Enzyme and pathway databases

    Reactomei REACT_198389. PCP/CE pathway.

    Miscellaneous databases

    NextBioi 294634.
    PROi P62963.

    Gene expression databases

    Genevestigatori P62963.

    Family and domain databases

    InterProi IPR005454. Profilin_chordates.
    IPR027310. Profilin_CS.
    IPR005455. Profilin_eukaryotes/bac.
    [Graphical view ]
    Pfami PF00235. Profilin. 1 hit.
    [Graphical view ]
    PRINTSi PR01639. PROFILINMAML.
    SMARTi SM00392. PROF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55770. SSF55770. 1 hit.
    PROSITEi PS00414. PROFILIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis."
      Tamura M., Tanaka H., Hirano T., Ueta Y., Higashi K., Hirano H.
      Biochem. Biophys. Res. Commun. 222:683-687(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 39-54; 57-70 AND 76-89, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiPROF1_RAT
    AccessioniPrimary (citable) accession number: P62963
    Secondary accession number(s): P10924
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3