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Protein

Profilin-1

Gene

Pfn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity).By similarity

GO - Molecular functioni

  1. actin binding Source: RGD
  2. adenyl-nucleotide exchange factor activity Source: Ensembl
  3. phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  4. poly(A) RNA binding Source: Ensembl
  5. receptor binding Source: RGD

GO - Biological processi

  1. actin polymerization or depolymerization Source: RGD
  2. cellular response to growth factor stimulus Source: RGD
  3. negative regulation of actin filament polymerization Source: Ensembl
  4. negative regulation of stress fiber assembly Source: Ensembl
  5. neural tube closure Source: Ensembl
  6. positive regulation of actin filament polymerization Source: Ensembl
  7. positive regulation of ATPase activity Source: Ensembl
  8. positive regulation of DNA metabolic process Source: RGD
  9. positive regulation of epithelial cell migration Source: Ensembl
  10. positive regulation of ruffle assembly Source: Ensembl
  11. positive regulation of stress fiber assembly Source: RGD
  12. positive regulation of transcription from RNA polymerase II promoter Source: RGD
  13. positive regulation of viral transcription Source: RGD
  14. response to organic substance Source: RGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_276901. Signaling by Robo receptor.
REACT_288006. Platelet degranulation.
REACT_345573. PCP/CE pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin-1
Alternative name(s):
Profilin I
Gene namesi
Name:Pfn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621825. Pfn1.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: Ensembl
  2. cytoplasm Source: RGD
  3. cytoskeleton Source: UniProtKB-SubCell
  4. extracellular vesicular exosome Source: Ensembl
  5. focal adhesion Source: Ensembl
  6. membrane Source: Ensembl
  7. neuron projection Source: RGD
  8. nucleus Source: Ensembl
  9. synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 140139Profilin-1PRO_0000199573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki54 – 54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei108 – 1081N6-acetyllysineBy similarity
Modified residuei129 – 1291PhosphotyrosineBy similarity
Modified residuei138 – 1381Phosphoserine; by ROCK1By similarity

Post-translational modificationi

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62963.
PRIDEiP62963.

PTM databases

PhosphoSiteiP62963.

Expressioni

Gene expression databases

GenevestigatoriP62963.

Interactioni

Subunit structurei

Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 (By similarity). Interacts with HTT (By similarity).By similarity

Protein-protein interaction databases

BioGridi249018. 4 interactions.
IntActiP62963. 2 interactions.
MINTiMINT-1868721.

Structurei

3D structure databases

ProteinModelPortaliP62963.
SMRiP62963. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the profilin family.Curated

Phylogenomic databases

eggNOGiNOG269129.
GeneTreeiENSGT00390000010143.
HOGENOMiHOG000171592.
HOVERGENiHBG053683.
InParanoidiP62963.
KOiK05759.
OMAiHLRRAQY.
OrthoDBiEOG7JMGGT.
PhylomeDBiP62963.
TreeFamiTF331744.

Family and domain databases

InterProiIPR005455. PFN.
IPR029892. PFN1.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR13936:SF7. PTHR13936:SF7. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR01639. PROFILINMAML.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV
60 70 80 90 100
LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN
110 120 130 140
VTVTMTAKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
Length:140
Mass (Da):14,957
Last modified:January 23, 2007 - v2
Checksum:i1DC8B60D545FF70E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96967 mRNA. Translation: CAA65655.1.
BC062405 mRNA. Translation: AAH62405.1.
PIRiPC4172.
RefSeqiNP_071956.2. NM_022511.2.
UniGeneiRn.1152.

Genome annotation databases

EnsembliENSRNOT00000005370; ENSRNOP00000005370; ENSRNOG00000003975.
GeneIDi64303.
KEGGirno:64303.
UCSCiRGD:621825. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96967 mRNA. Translation: CAA65655.1.
BC062405 mRNA. Translation: AAH62405.1.
PIRiPC4172.
RefSeqiNP_071956.2. NM_022511.2.
UniGeneiRn.1152.

3D structure databases

ProteinModelPortaliP62963.
SMRiP62963. Positions 2-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249018. 4 interactions.
IntActiP62963. 2 interactions.
MINTiMINT-1868721.

PTM databases

PhosphoSiteiP62963.

Proteomic databases

PaxDbiP62963.
PRIDEiP62963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005370; ENSRNOP00000005370; ENSRNOG00000003975.
GeneIDi64303.
KEGGirno:64303.
UCSCiRGD:621825. rat.

Organism-specific databases

CTDi5216.
RGDi621825. Pfn1.

Phylogenomic databases

eggNOGiNOG269129.
GeneTreeiENSGT00390000010143.
HOGENOMiHOG000171592.
HOVERGENiHBG053683.
InParanoidiP62963.
KOiK05759.
OMAiHLRRAQY.
OrthoDBiEOG7JMGGT.
PhylomeDBiP62963.
TreeFamiTF331744.

Enzyme and pathway databases

ReactomeiREACT_276901. Signaling by Robo receptor.
REACT_288006. Platelet degranulation.
REACT_345573. PCP/CE pathway.

Miscellaneous databases

NextBioi294634.
PROiP62963.

Gene expression databases

GenevestigatoriP62963.

Family and domain databases

InterProiIPR005455. PFN.
IPR029892. PFN1.
IPR005454. Profilin1/2/3_vertebrate.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR13936:SF7. PTHR13936:SF7. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR01639. PROFILINMAML.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis."
    Tamura M., Tanaka H., Hirano T., Ueta Y., Higashi K., Hirano H.
    Biochem. Biophys. Res. Commun. 222:683-687(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 39-54; 57-70 AND 76-89, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiPROF1_RAT
AccessioniPrimary (citable) accession number: P62963
Secondary accession number(s): P10924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.