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P62962

- PROF1_MOUSE

UniProt

P62962 - PROF1_MOUSE

Protein

Profilin-1

Gene

Pfn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR By similarity.By similarity

    GO - Molecular functioni

    1. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
    2. protein binding Source: IntAct
    3. Rho GTPase binding Source: BHF-UCL

    GO - Biological processi

    1. actin cytoskeleton organization Source: InterPro
    2. cellular response to growth factor stimulus Source: Ensembl
    3. neural tube closure Source: MGI
    4. positive regulation of DNA metabolic process Source: Ensembl
    5. positive regulation of stress fiber assembly Source: Ensembl
    6. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    7. positive regulation of viral transcription Source: Ensembl
    8. regulation of actin polymerization or depolymerization Source: MGI
    9. regulation of transcription from RNA polymerase II promoter Source: MGI
    10. sequestering of actin monomers Source: MGI

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_214043. PCP/CE pathway.
    REACT_221567. Signaling by Robo receptor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Profilin-1
    Alternative name(s):
    Profilin I
    Gene namesi
    Name:Pfn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:97549. Pfn1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytoskeleton Source: UniProtKB-SubCell
    3. cytosol Source: Reactome
    4. extracellular region Source: Reactome
    5. neuron projection Source: Ensembl
    6. nucleus Source: MGI
    7. synapse Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 140139Profilin-1PRO_0000199572Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Cross-linki54 – 54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei108 – 1081N6-acetyllysineBy similarity
    Modified residuei129 – 1291PhosphotyrosineBy similarity
    Modified residuei138 – 1381Phosphoserine; by ROCK1By similarity

    Post-translational modificationi

    Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP62962.
    PaxDbiP62962.
    PRIDEiP62962.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00224740.
    P62962.

    PTM databases

    PhosphoSiteiP62962.

    Expressioni

    Gene expression databases

    ArrayExpressiP62962.
    BgeeiP62962.
    CleanExiMM_PFN1.
    GenevestigatoriP62962.

    Interactioni

    Subunit structurei

    Interacts with VASP. Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1 By similarity. Interacts with HTT By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ESR1P033723EBI-647096,EBI-78473From a different organism.

    Protein-protein interaction databases

    BioGridi202126. 11 interactions.
    DIPiDIP-38075N.
    IntActiP62962. 6 interactions.
    MINTiMINT-1868701.

    Structurei

    3D structure databases

    ProteinModelPortaliP62962.
    SMRiP62962. Positions 2-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the profilin family.Curated

    Phylogenomic databases

    eggNOGiNOG269129.
    HOGENOMiHOG000171592.
    HOVERGENiHBG053683.
    InParanoidiP62962.
    KOiK05759.
    OrthoDBiEOG7JMGGT.
    PhylomeDBiP62962.
    TreeFamiTF331744.

    Family and domain databases

    InterProiIPR005454. Profilin_chordates.
    IPR027310. Profilin_CS.
    IPR005455. Profilin_eukaryotes/bac.
    [Graphical view]
    PfamiPF00235. Profilin. 1 hit.
    [Graphical view]
    PRINTSiPR01639. PROFILINMAML.
    SMARTiSM00392. PROF. 1 hit.
    [Graphical view]
    SUPFAMiSSF55770. SSF55770. 1 hit.
    PROSITEiPS00414. PROFILIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62962-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV    50
    LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN 100
    VTVTMTAKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY 140
    Length:140
    Mass (Da):14,957
    Last modified:January 23, 2007 - v2
    Checksum:i1DC8B60D545FF70E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14425 mRNA. Translation: CAA32586.1.
    AK011066 mRNA. Translation: BAB27373.1.
    BC002080 mRNA. Translation: AAH02080.1.
    CCDSiCCDS24960.1.
    PIRiS04067.
    RefSeqiNP_035202.1. NM_011072.4.
    UniGeneiMm.2647.

    Genome annotation databases

    EnsembliENSMUST00000018437; ENSMUSP00000018437; ENSMUSG00000018293.
    GeneIDi18643.
    KEGGimmu:18643.
    UCSCiuc007jvv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14425 mRNA. Translation: CAA32586.1 .
    AK011066 mRNA. Translation: BAB27373.1 .
    BC002080 mRNA. Translation: AAH02080.1 .
    CCDSi CCDS24960.1.
    PIRi S04067.
    RefSeqi NP_035202.1. NM_011072.4.
    UniGenei Mm.2647.

    3D structure databases

    ProteinModelPortali P62962.
    SMRi P62962. Positions 2-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202126. 11 interactions.
    DIPi DIP-38075N.
    IntActi P62962. 6 interactions.
    MINTi MINT-1868701.

    PTM databases

    PhosphoSitei P62962.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00224740.
    P62962.

    Proteomic databases

    MaxQBi P62962.
    PaxDbi P62962.
    PRIDEi P62962.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018437 ; ENSMUSP00000018437 ; ENSMUSG00000018293 .
    GeneIDi 18643.
    KEGGi mmu:18643.
    UCSCi uc007jvv.2. mouse.

    Organism-specific databases

    CTDi 5216.
    MGIi MGI:97549. Pfn1.

    Phylogenomic databases

    eggNOGi NOG269129.
    HOGENOMi HOG000171592.
    HOVERGENi HBG053683.
    InParanoidi P62962.
    KOi K05759.
    OrthoDBi EOG7JMGGT.
    PhylomeDBi P62962.
    TreeFami TF331744.

    Enzyme and pathway databases

    Reactomei REACT_214043. PCP/CE pathway.
    REACT_221567. Signaling by Robo receptor.

    Miscellaneous databases

    NextBioi 294634.
    PROi P62962.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62962.
    Bgeei P62962.
    CleanExi MM_PFN1.
    Genevestigatori P62962.

    Family and domain databases

    InterProi IPR005454. Profilin_chordates.
    IPR027310. Profilin_CS.
    IPR005455. Profilin_eukaryotes/bac.
    [Graphical view ]
    Pfami PF00235. Profilin. 1 hit.
    [Graphical view ]
    PRINTSi PR01639. PROFILINMAML.
    SMARTi SM00392. PROF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55770. SSF55770. 1 hit.
    PROSITEi PS00414. PROFILIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Total coding sequence of profilin cDNA from Mus musculus macrophage."
      Sri Widada J., Ferraz C., Liautard J.P.
      Nucleic Acids Res. 17:2855-2855(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    4. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 39-54; 57-70 AND 76-89, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiPROF1_MOUSE
    AccessioniPrimary (citable) accession number: P62962
    Secondary accession number(s): P10924
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3