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Protein

Histidine triad nucleotide-binding protein 1

Gene

Hint1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99Purine nucleotide phosphoramidateBy similarity1
Active sitei112Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 44Purine nucleotide phosphoramidateBy similarity2
Nucleotide bindingi105 – 107Purine nucleotide phosphoramidateBy similarity3
Nucleotide bindingi112 – 114Purine nucleotide phosphoramidateBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine triad nucleotide-binding protein 1 (EC:3.-.-.-)
Alternative name(s):
17 kDa inhibitor of protein kinase C
Adenosine 5'-monophosphoramidase
Protein kinase C inhibitor 1
Protein kinase C-interacting protein 1
Short name:
PKCI-1
Gene namesi
Name:Hint1
Synonyms:Hint, Pkci1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1593411. Hint1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001097842 – 126Histidine triad nucleotide-binding protein 1Add BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei21N6-acetyllysineBy similarity1
Modified residuei30N6-acetyllysineBy similarity1
Modified residuei45PhosphoserineBy similarity1
Modified residuei72PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62959.
PRIDEiP62959.

PTM databases

iPTMnetiP62959.
PhosphoSitePlusiP62959.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000622.
GenevisibleiP62959. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with CDK7. Interacts with RUVBL1 and RUVBL2 and is associated with the LEF1/TCF1-CTNNB1 complex and with a KAT5 histone acetyltransferase complex. Identified in a complex with MITF and CTNNB1. Interacts with CDC34 and RBX1, and is part of a SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000772.

Chemistry databases

BindingDBiP62959.

Structurei

3D structure databases

ProteinModelPortaliP62959.
SMRiP62959.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 126HITPROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi110 – 114Histidine triad motif5

Sequence similaritiesi

Belongs to the HINT family.Curated
Contains 1 HIT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3275. Eukaryota.
COG0537. LUCA.
GeneTreeiENSGT00510000046448.
HOGENOMiHOG000061064.
HOVERGENiHBG051906.
InParanoidiP62959.
KOiK02503.
OMAiYRLITNN.
OrthoDBiEOG091G1001.
PhylomeDBiP62959.
TreeFamiTF314862.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiPF01230. HIT. 1 hit.
[Graphical view]
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEIAKAQV AQPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT
60 70 80 90 100
HFLVIPKKHI SQISVADDDD ESLLGHLMIV GKKCAADLGL KRGYRMVVNE
110 120
GADGGQSVYH IHLHVLGGRQ MNWPPG
Length:126
Mass (Da):13,777
Last modified:March 3, 2009 - v5
Checksum:i30DA241476389805
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12Q → R in AAA18398 (Ref. 1) Curated1
Sequence conflicti33F → Y in AAA18398 (Ref. 1) Curated1
Sequence conflicti37R → Q in AAA18398 (Ref. 1) Curated1
Sequence conflicti59H → Y in AAA18398 (Ref. 1) Curated1
Sequence conflicti65V → A in AAA18398 (Ref. 1) Curated1
Sequence conflicti67D → E in AAA18398 (Ref. 1) Curated1
Sequence conflicti92R → K in AAA18398 (Ref. 1) Curated1
Sequence conflicti102A → S in AAA18398 (Ref. 1) Curated1
Sequence conflicti111I → V in AAA18398 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09407 mRNA. Translation: AAA18398.1.
CH473948 Genomic DNA. Translation: EDM04446.1.
BC168732 mRNA. Translation: AAI68732.1.
PIRiA59489.
RefSeqiNP_001103077.1. NM_001109607.1.
UniGeneiRn.138667.

Genome annotation databases

EnsembliENSRNOT00000000772; ENSRNOP00000000772; ENSRNOG00000000622.
GeneIDi690660.
KEGGirno:690660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09407 mRNA. Translation: AAA18398.1.
CH473948 Genomic DNA. Translation: EDM04446.1.
BC168732 mRNA. Translation: AAI68732.1.
PIRiA59489.
RefSeqiNP_001103077.1. NM_001109607.1.
UniGeneiRn.138667.

3D structure databases

ProteinModelPortaliP62959.
SMRiP62959.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000772.

Chemistry databases

BindingDBiP62959.

PTM databases

iPTMnetiP62959.
PhosphoSitePlusiP62959.

Proteomic databases

PaxDbiP62959.
PRIDEiP62959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000772; ENSRNOP00000000772; ENSRNOG00000000622.
GeneIDi690660.
KEGGirno:690660.

Organism-specific databases

CTDi3094.
RGDi1593411. Hint1.

Phylogenomic databases

eggNOGiKOG3275. Eukaryota.
COG0537. LUCA.
GeneTreeiENSGT00510000046448.
HOGENOMiHOG000061064.
HOVERGENiHBG051906.
InParanoidiP62959.
KOiK02503.
OMAiYRLITNN.
OrthoDBiEOG091G1001.
PhylomeDBiP62959.
TreeFamiTF314862.

Miscellaneous databases

PROiP62959.

Gene expression databases

BgeeiENSRNOG00000000622.
GenevisibleiP62959. RN.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiPF01230. HIT. 1 hit.
[Graphical view]
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHINT1_RAT
AccessioniPrimary (citable) accession number: P62959
Secondary accession number(s): B5DEM9, P16436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 95 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a protein kinase C inhibitor and to bind zinc in solution. Both seem to be incorrect.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.