P62917 (RL8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L8

Gene names

Name:

RPL8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm.
Post-translational modification	Hydroxylated on His-216 by NO66. The modification is impaired by hypoxia.
Miscellaneous	This protein can be partially incorporated into E.coli polysomes in vivo, indicating it can replace the endogenous protein.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Hydroxylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: Reactome translational elongation Traceable author statement. Source: Reactome translational initiation Traceable author statement. Source: Reactome translational termination Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay Ref.9. Source: UniProtKB
Molecular_function	RNA binding Traceable author statement Ref.1. Source: ProtInc rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Non-traceable author statement Ref.9. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.9
	Chain	2 – 257	256	60S ribosomal protein L8		PRO_0000129743
Amino acid modifications
	Modified residue	216	1	(3S)-3-hydroxyhistidine
Natural variations
	Natural variant	98	1	I → V. Ref.3 Ref.7 Corresponds to variant rs17850886 [ dbSNP | Ensembl ].		VAR_019658
Experimental info
	Mutagenesis	209	1	H → A or G: No incorporation into translating E.coli polysomes; ribosomes assembled normally. Significantly reduced translational activity.

Sequences

	Sequence		Length	Mass (Da)
	P62917 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CE1610449749318B	FASTA	257	28,025
	10 20 30 40 50 60 MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK 70 80 90 100 110 120 VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP 130 140 150 160 170 180 GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL 190 200 210 220 230 240 KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA 250 RRTGRLRGTK TVQEKEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization by cDNA cloning of the mRNA of human ribosomal protein L8." Hanes J., Klaudiny J., von der Kammer H., Scheit K.H. Biochem. Biophys. Res. Commun. 197:1223-1228(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"The human ribosomal protein genes: sequencing and comparative analysis of 73 genes." Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S., Maeda N., Minoshima S., Tanaka T., Shimizu N., Kenmochi N. Genome Res. 12:379-390(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-98.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-98. Tissue: Kidney, Placenta, Skin and Spinal cord.
[8]	"A map of 75 human ribosomal protein genes." Kenmochi N., Kawaguchi T., Rozen S., Davis E., Goodman N., Hudson T.J., Tanaka T., Page D.C. Genome Res. 8:509-523(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-134.
[9]	"Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing." Odintsova T.I., Muller E.C., Ivanov A.V., Egorov T.A., Bienert R., Vladimirov S.N., Kostka S., Otto A., Wittmann-Liebold B., Karpova G.G. J. Protein Chem. 22:249-258(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, MASS SPECTROMETRY.
[10]	"Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies." Uehlein M., Wegloehner W., Urlaub H., Wittmann-Liebold B. Biochem. J. 331:423-430(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN REPLACEMENT STUDIES IN E.COLI, MUTAGENESIS.
[11]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans." Ge W., Wolf A., Feng T., Ho C.H., Sekirnik R., Zayer A., Granatino N., Cockman M.E., Loenarz C., Loik N.D., Hardy A.P., Claridge T.D., Hamed R.B., Chowdhury R., Gong L., Robinson C.V., Trudgian D.C., Jiang M. , Mackeen M.M., McCullagh J.S., Gordiyenko Y., Thalhammer A., Yamamoto A., Yang M., Liu-Yi P., Zhang Z., Schmidt-Zachmann M., Kessler B.M., Ratcliffe P.J., Preston G.M., Coleman M.L., Schofield C.J. Nat. Chem. Biol. 8:960-962(2012) [PubMed] [Europe PMC] [Abstract] Cited for: HYDROXYLATION AT HIS-216 BY NO66.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28407 mRNA. Translation: CAA82248.1. AB061821 Genomic DNA. Translation: BAB79459.1. BT007379 mRNA. Translation: AAP36043.1. CR457046 mRNA. Translation: CAG33327.1. AK289459 mRNA. Translation: BAF82148.1. CH471162 Genomic DNA. Translation: EAW82048.1. CH471162 Genomic DNA. Translation: EAW82051.1. CH471162 Genomic DNA. Translation: EAW82052.1. BC000047 mRNA. Translation: AAH00047.2. BC000077 mRNA. Translation: AAH00077.1. BC012197 mRNA. Translation: AAH12197.1. BC013104 mRNA. Translation: AAH13104.1. BC093064 mRNA. Translation: AAH93064.1. AB007168 Genomic DNA. Translation: BAA25829.1.
IPI	IPI00012772.
PIR	JN0923.
RefSeq	NP_000964.1. NM_000973.3. NP_150644.1. NM_033301.1.
UniGene	Hs.178551.
3D structure databases
ProteinModelPortal	P62917.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62917. 24 interactions.
MINT	MINT-1135693.
STRING	9606.ENSP00000262584.
PTM databases
PhosphoSite	P62917.
Polymorphism databases
DMDM	51702823.
Proteomic databases
PaxDb	P62917.
PRIDE	P62917.
Protocols and materials databases
DNASU	6132.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262584; ENSP00000262584; ENSG00000161016. ENST00000394920; ENSP00000378378; ENSG00000161016.
GeneID	6132.
KEGG	hsa:6132.
UCSC	uc003zeb.3. human.
Organism-specific databases
CTD	6132.
GeneCards	GC08M145985.
HGNC	HGNC:10368. RPL8.
HPA	HPA045095. HPA050165.
MIM	604177. gene.
neXtProt	NX_P62917.
PharmGKB	PA34768.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0090.
HOVERGEN	HBG079431.
InParanoid	P62917.
KO	K02938.
OMA	GRVIRNQ.
OrthoDB	EOG4XKV7P.
PhylomeDB	P62917.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P62917.
Bgee	P62917.
CleanEx	HS_RPL8.
Genevestigator	P62917.
GermOnline	ENSG00000161016. Homo sapiens.
Family and domain databases
Gene3D	2.30.30.30. 1 hit. 2.40.50.140. 1 hit. 4.10.950.10. 1 hit.
InterPro	IPR012340. NA-bd_OB-fold. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR014722. Rib_L2_dom2. IPR002171. Ribosomal_L2. IPR023672. Ribosomal_L2_arc. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014726. Ribosomal_L2_dom3. IPR008991. Translation_prot_SH3-like. [Graphical view]
PANTHER	PTHR13691. PTHR13691. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPL8. human.
GenomeRNAi	6132.
NextBio	23815.
SOURCE	Search...

Entry information

Entry name

RL8_HUMAN

Accession

Primary (citable) accession number: P62917
Secondary accession number(s): A8K094 , D3DWN2, P25120, Q567Q7, Q969V7, Q9BWQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.