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P62917

- RL8_HUMAN

UniProt

P62917 - RL8_HUMAN

Protein

60S ribosomal protein L8

Gene

RPL8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. RNA binding Source: ProtInc
    3. rRNA binding Source: UniProtKB-KW
    4. structural constituent of ribosome Source: UniProtKB

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. gene expression Source: Reactome
    3. mRNA metabolic process Source: Reactome
    4. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
    5. RNA metabolic process Source: Reactome
    6. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
    7. translation Source: UniProtKB
    8. translational elongation Source: Reactome
    9. translational initiation Source: Reactome
    10. translational termination Source: Reactome
    11. viral life cycle Source: Reactome
    12. viral process Source: Reactome
    13. viral transcription Source: Reactome

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
    REACT_1404. Peptide chain elongation.
    REACT_1797. Formation of a pool of free 40S subunits.
    REACT_1986. Eukaryotic Translation Termination.
    REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
    REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
    REACT_9491. Viral mRNA Translation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L8
    Gene namesi
    Name:RPL8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:10368. RPL8.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. cytosolic large ribosomal subunit Source: UniProtKB
    3. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi209 – 2091H → A or G: No incorporation into translating E.coli polysomes; ribosomes assembled normally. Significantly reduced translational activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA34768.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 25725660S ribosomal protein L8PRO_0000129743Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei216 – 2161(3S)-3-hydroxyhistidine1 Publication

    Post-translational modificationi

    Hydroxylated on His-216 by NO66. The modification is impaired by hypoxia.1 Publication

    Keywords - PTMi

    Hydroxylation

    Proteomic databases

    MaxQBiP62917.
    PaxDbiP62917.
    PRIDEiP62917.

    PTM databases

    PhosphoSiteiP62917.

    Expressioni

    Gene expression databases

    ArrayExpressiP62917.
    BgeeiP62917.
    CleanExiHS_RPL8.
    GenevestigatoriP62917.

    Organism-specific databases

    HPAiHPA045095.
    HPA050165.

    Interactioni

    Protein-protein interaction databases

    BioGridi112052. 107 interactions.
    DIPiDIP-37967N.
    IntActiP62917. 34 interactions.
    MINTiMINT-1135693.
    STRINGi9606.ENSP00000262584.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3J3Belectron microscopy5.00A1-257[»]
    4CCMX-ray2.51C/D205-239[»]
    4CCNX-ray2.23C/D205-239[»]
    4CCOX-ray2.30C/D205-224[»]
    ProteinModelPortaliP62917.
    SMRiP62917. Positions 2-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L2P family.Curated

    Phylogenomic databases

    eggNOGiCOG0090.
    HOVERGENiHBG079431.
    InParanoidiP62917.
    KOiK02938.
    OMAiVDILHDP.
    PhylomeDBiP62917.
    TreeFamiTF300748.

    Family and domain databases

    Gene3Di2.30.30.30. 1 hit.
    2.40.50.140. 1 hit.
    4.10.950.10. 1 hit.
    HAMAPiMF_01320_A. Ribosomal_L2_A.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR022666. Rbsml_prot_L2_RNA-bd_dom.
    IPR014722. Rib_L2_dom2.
    IPR002171. Ribosomal_L2.
    IPR023672. Ribosomal_L2_arc.
    IPR022669. Ribosomal_L2_C.
    IPR022671. Ribosomal_L2_CS.
    IPR014726. Ribosomal_L2_dom3.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view]
    PANTHERiPTHR13691. PTHR13691. 1 hit.
    PfamiPF00181. Ribosomal_L2. 1 hit.
    PF03947. Ribosomal_L2_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
    SUPFAMiSSF50104. SSF50104. 1 hit.
    SSF50249. SSF50249. 1 hit.
    PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62917-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH    50
    DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN 100
    VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL 150
    PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR 200
    GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK 250
    TVQEKEN 257
    Length:257
    Mass (Da):28,025
    Last modified:January 23, 2007 - v2
    Checksum:iCE1610449749318B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti98 – 981I → V.2 Publications
    Corresponds to variant rs17850886 [ dbSNP | Ensembl ].
    VAR_019658

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28407 mRNA. Translation: CAA82248.1.
    AB061821 Genomic DNA. Translation: BAB79459.1.
    BT007379 mRNA. Translation: AAP36043.1.
    CR457046 mRNA. Translation: CAG33327.1.
    AK289459 mRNA. Translation: BAF82148.1.
    CH471162 Genomic DNA. Translation: EAW82048.1.
    CH471162 Genomic DNA. Translation: EAW82051.1.
    CH471162 Genomic DNA. Translation: EAW82052.1.
    BC000047 mRNA. Translation: AAH00047.2.
    BC000077 mRNA. Translation: AAH00077.1.
    BC012197 mRNA. Translation: AAH12197.1.
    BC013104 mRNA. Translation: AAH13104.1.
    BC093064 mRNA. Translation: AAH93064.1.
    AB007168 Genomic DNA. Translation: BAA25829.1.
    CCDSiCCDS6433.1.
    PIRiJN0923.
    RefSeqiNP_000964.1. NM_000973.3.
    NP_150644.1. NM_033301.1.
    UniGeneiHs.178551.

    Genome annotation databases

    EnsembliENST00000262584; ENSP00000262584; ENSG00000161016.
    ENST00000394920; ENSP00000378378; ENSG00000161016.
    ENST00000528957; ENSP00000433464; ENSG00000161016.
    GeneIDi6132.
    KEGGihsa:6132.
    UCSCiuc003zeb.3. human.

    Polymorphism databases

    DMDMi51702823.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28407 mRNA. Translation: CAA82248.1 .
    AB061821 Genomic DNA. Translation: BAB79459.1 .
    BT007379 mRNA. Translation: AAP36043.1 .
    CR457046 mRNA. Translation: CAG33327.1 .
    AK289459 mRNA. Translation: BAF82148.1 .
    CH471162 Genomic DNA. Translation: EAW82048.1 .
    CH471162 Genomic DNA. Translation: EAW82051.1 .
    CH471162 Genomic DNA. Translation: EAW82052.1 .
    BC000047 mRNA. Translation: AAH00047.2 .
    BC000077 mRNA. Translation: AAH00077.1 .
    BC012197 mRNA. Translation: AAH12197.1 .
    BC013104 mRNA. Translation: AAH13104.1 .
    BC093064 mRNA. Translation: AAH93064.1 .
    AB007168 Genomic DNA. Translation: BAA25829.1 .
    CCDSi CCDS6433.1.
    PIRi JN0923.
    RefSeqi NP_000964.1. NM_000973.3.
    NP_150644.1. NM_033301.1.
    UniGenei Hs.178551.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3J3B electron microscopy 5.00 A 1-257 [» ]
    4CCM X-ray 2.51 C/D 205-239 [» ]
    4CCN X-ray 2.23 C/D 205-239 [» ]
    4CCO X-ray 2.30 C/D 205-224 [» ]
    ProteinModelPortali P62917.
    SMRi P62917. Positions 2-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112052. 107 interactions.
    DIPi DIP-37967N.
    IntActi P62917. 34 interactions.
    MINTi MINT-1135693.
    STRINGi 9606.ENSP00000262584.

    PTM databases

    PhosphoSitei P62917.

    Polymorphism databases

    DMDMi 51702823.

    Proteomic databases

    MaxQBi P62917.
    PaxDbi P62917.
    PRIDEi P62917.

    Protocols and materials databases

    DNASUi 6132.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262584 ; ENSP00000262584 ; ENSG00000161016 .
    ENST00000394920 ; ENSP00000378378 ; ENSG00000161016 .
    ENST00000528957 ; ENSP00000433464 ; ENSG00000161016 .
    GeneIDi 6132.
    KEGGi hsa:6132.
    UCSCi uc003zeb.3. human.

    Organism-specific databases

    CTDi 6132.
    GeneCardsi GC08M145985.
    HGNCi HGNC:10368. RPL8.
    HPAi HPA045095.
    HPA050165.
    MIMi 604177. gene.
    neXtProti NX_P62917.
    PharmGKBi PA34768.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0090.
    HOVERGENi HBG079431.
    InParanoidi P62917.
    KOi K02938.
    OMAi VDILHDP.
    PhylomeDBi P62917.
    TreeFami TF300748.

    Enzyme and pathway databases

    Reactomei REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
    REACT_1404. Peptide chain elongation.
    REACT_1797. Formation of a pool of free 40S subunits.
    REACT_1986. Eukaryotic Translation Termination.
    REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
    REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
    REACT_9491. Viral mRNA Translation.

    Miscellaneous databases

    ChiTaRSi RPL8. human.
    GeneWikii RPL8.
    GenomeRNAii 6132.
    NextBioi 23815.
    PROi P62917.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62917.
    Bgeei P62917.
    CleanExi HS_RPL8.
    Genevestigatori P62917.

    Family and domain databases

    Gene3Di 2.30.30.30. 1 hit.
    2.40.50.140. 1 hit.
    4.10.950.10. 1 hit.
    HAMAPi MF_01320_A. Ribosomal_L2_A.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR022666. Rbsml_prot_L2_RNA-bd_dom.
    IPR014722. Rib_L2_dom2.
    IPR002171. Ribosomal_L2.
    IPR023672. Ribosomal_L2_arc.
    IPR022669. Ribosomal_L2_C.
    IPR022671. Ribosomal_L2_CS.
    IPR014726. Ribosomal_L2_dom3.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view ]
    PANTHERi PTHR13691. PTHR13691. 1 hit.
    Pfami PF00181. Ribosomal_L2. 1 hit.
    PF03947. Ribosomal_L2_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002158. Ribosomal_L2. 1 hit.
    SUPFAMi SSF50104. SSF50104. 1 hit.
    SSF50249. SSF50249. 1 hit.
    PROSITEi PS00467. RIBOSOMAL_L2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization by cDNA cloning of the mRNA of human ribosomal protein L8."
      Hanes J., Klaudiny J., von der Kammer H., Scheit K.H.
      Biochem. Biophys. Res. Commun. 197:1223-1228(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.
    2. "The human ribosomal protein genes: sequencing and comparative analysis of 73 genes."
      Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S., Maeda N., Minoshima S., Tanaka T., Shimizu N., Kenmochi N.
      Genome Res. 12:379-390(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-98.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-98.
      Tissue: Kidney, Placenta, Skin and Spinal cord.
    8. "A map of 75 human ribosomal protein genes."
      Kenmochi N., Kawaguchi T., Rozen S., Davis E., Goodman N., Hudson T.J., Tanaka T., Page D.C.
      Genome Res. 8:509-523(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-134.
    9. "Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing."
      Odintsova T.I., Muller E.C., Ivanov A.V., Egorov T.A., Bienert R., Vladimirov S.N., Kostka S., Otto A., Wittmann-Liebold B., Karpova G.G.
      J. Protein Chem. 22:249-258(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies."
      Uehlein M., Wegloehner W., Urlaub H., Wittmann-Liebold B.
      Biochem. J. 331:423-430(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN REPLACEMENT STUDIES IN E.COLI, MUTAGENESIS.
    11. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: HYDROXYLATION AT HIS-216 BY NO66.
    14. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).

    Entry informationi

    Entry nameiRL8_HUMAN
    AccessioniPrimary (citable) accession number: P62917
    Secondary accession number(s): A8K094
    , D3DWN2, P25120, Q567Q7, Q969V7, Q9BWQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This protein can be partially incorporated into E.coli polysomes in vivo, indicating it can replace the endogenous protein.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Ribosomal proteins
      Ribosomal proteins families and list of entries
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3