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Protein

Transcription initiation factor IIB

Gene

Gtf2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151ZincBy similarity
Metal bindingi18 – 181ZincBy similarity
Metal bindingi34 – 341ZincBy similarity
Metal bindingi37 – 371ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 4232TFIIB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core promoter binding Source: MGI
  2. DNA binding Source: MGI
  3. thyroid hormone receptor binding Source: MGI
  4. transcription factor binding Source: MGI
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA-templated transcription, initiation Source: InterPro
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_230204. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_232708. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_244380. RNA Polymerase II Transcription Initiation.
REACT_255009. RNA Polymerase II Pre-transcription Events.
REACT_255334. RNA Polymerase II Promoter Escape.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
RNA polymerase II alpha initiation factor
Gene namesi
Name:Gtf2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:2385191. Gtf2b.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleolus Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Transcription initiation factor IIBPRO_0000119294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP62915.
PaxDbiP62915.
PRIDEiP62915.

PTM databases

PhosphoSiteiP62915.

Expressioni

Gene expression databases

BgeeiP62915.
CleanExiMM_GTF2B.
ExpressionAtlasiP62915. baseline and differential.
GenevestigatoriP62915.

Interactioni

Subunit structurei

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2 (By similarity). Interacts with GPBP1.By similarity1 Publication

Protein-protein interaction databases

BioGridi230917. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliP62915.
SMRiP62915. Positions 2-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati124 – 200771Add
BLAST
Repeati218 – 294772Add
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 4232TFIIB-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG1405.
HOGENOMiHOG000108634.
HOVERGENiHBG017914.
InParanoidiP62915.
KOiK03124.
OMAiWRAFDHD.
OrthoDBiEOG78WKS2.
PhylomeDBiP62915.
TreeFamiTF105953.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS
60 70 80 90 100
EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK
110 120 130 140 150
YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS
160 170 180 190 200
LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK
210 220 230 240 250
ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP
260 270 280 290 300
ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
310
PSDFKFDTPV DKLPQL
Length:316
Mass (Da):34,819
Last modified:August 31, 2004 - v1
Checksum:i9CC7E102526C21F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC016637 mRNA. Translation: AAH16637.1.
CCDSiCCDS17882.1.
RefSeqiNP_663521.1. NM_145546.1.
UniGeneiMm.271756.

Genome annotation databases

EnsembliENSMUST00000029938; ENSMUSP00000029938; ENSMUSG00000028271.
GeneIDi229906.
KEGGimmu:229906.
UCSCiuc008rpb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC016637 mRNA. Translation: AAH16637.1.
CCDSiCCDS17882.1.
RefSeqiNP_663521.1. NM_145546.1.
UniGeneiMm.271756.

3D structure databases

ProteinModelPortaliP62915.
SMRiP62915. Positions 2-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230917. 7 interactions.

PTM databases

PhosphoSiteiP62915.

Proteomic databases

MaxQBiP62915.
PaxDbiP62915.
PRIDEiP62915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029938; ENSMUSP00000029938; ENSMUSG00000028271.
GeneIDi229906.
KEGGimmu:229906.
UCSCiuc008rpb.1. mouse.

Organism-specific databases

CTDi2959.
MGIiMGI:2385191. Gtf2b.

Phylogenomic databases

eggNOGiCOG1405.
HOGENOMiHOG000108634.
HOVERGENiHBG017914.
InParanoidiP62915.
KOiK03124.
OMAiWRAFDHD.
OrthoDBiEOG78WKS2.
PhylomeDBiP62915.
TreeFamiTF105953.

Enzyme and pathway databases

ReactomeiREACT_230204. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_232708. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_244380. RNA Polymerase II Transcription Initiation.
REACT_255009. RNA Polymerase II Pre-transcription Events.
REACT_255334. RNA Polymerase II Promoter Escape.

Miscellaneous databases

ChiTaRSiGtf2b. mouse.
NextBioi379735.
PROiP62915.
SOURCEiSearch...

Gene expression databases

BgeeiP62915.
CleanExiMM_GTF2B.
ExpressionAtlasiP62915. baseline and differential.
GenevestigatoriP62915.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription."
    Hsu L.-C., Liu S., Abedinpour F., Beech R.D., Lahti J.M., Kidd V.J., Greenspan J.A., Yeung C.-Y.
    Mol. Cell. Biol. 23:8773-8785(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPBP1.

Entry informationi

Entry nameiTF2B_MOUSE
AccessioniPrimary (citable) accession number: P62915
Secondary accession number(s): P29053
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: March 4, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.