P62915 (TF2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIB

Alternative name(s):
General transcription factor TFIIB
RNA polymerase II alpha initiation factor

Gene names

Name:

Gtf2b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2 By similarity. Interacts with GPBP1. Ref.2
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 18722179. Source: MGI
Molecular_function	core promoter binding Inferred from direct assay PubMed 22323595. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Transcription initiation factor IIB		PRO_0000119294
Regions
	Repeat	124 – 200	77	1
	Repeat	218 – 294	77	2
	Zinc finger	11 – 42	32	TFIIB-type
Sites
	Metal binding	15	1	Zinc By similarity
	Metal binding	18	1	Zinc By similarity
	Metal binding	34	1	Zinc By similarity
	Metal binding	37	1	Zinc By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62915 [UniParc]. Last modified August 31, 2004. Version 1. Checksum: 9CC7E102526C21F9	FASTA	316	34,819
	10 20 30 40 50 60 MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA 70 80 90 100 110 120 TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE 130 140 150 160 170 180 ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI 190 200 210 220 230 240 CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV 250 260 270 280 290 300 ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF 310 PSDFKFDTPV DKLPQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription." Hsu L.-C., Liu S., Abedinpour F., Beech R.D., Lahti J.M., Kidd V.J., Greenspan J.A., Yeung C.-Y. Mol. Cell. Biol. 23:8773-8785(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC016637 mRNA. Translation: AAH16637.1.
IPI	IPI00153874.
RefSeq	NP_663521.1. NM_145546.1.
UniGene	Mm.271756.
3D structure databases
ProteinModelPortal	P62915.
SMR	P62915. Positions 2-316.
ModBase	Search...
PTM databases
PhosphoSite	P62915.
Proteomic databases
PaxDb	P62915.
PRIDE	P62915.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029938; ENSMUSP00000029938; ENSMUSG00000028271.
GeneID	229906.
KEGG	mmu:229906.
Organism-specific databases
CTD	2959.
MGI	MGI:2385191. Gtf2b.
Phylogenomic databases
eggNOG	COG1405.
HOGENOM	HOG000108634.
HOVERGEN	HBG017914.
InParanoid	P62915.
KO	K03124.
OMA	WRAFDPD.
OrthoDB	EOG4QRH4F.
Gene expression databases
ArrayExpress	P62915.
Bgee	P62915.
CleanEx	MM_GTF2B.
Genevestigator	P62915.
GermOnline	ENSMUSG00000028271. Mus musculus.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR000812. TFIIB. IPR023486. TFIIB_CS. IPR013150. TFIIB_cyclin. IPR013137. Znf_TFIIB. [Graphical view]
PANTHER	PTHR11618. PTHR11618. 1 hit.
Pfam	PF08271. TF_Zn_Ribbon. 1 hit. PF00382. TFIIB. 2 hits. [Graphical view]
PRINTS	PR00685. TIFACTORIIB.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00782. TFIIB. 2 hits. PS51134. ZF_TFIIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	379735.
SOURCE	Search...

Entry information

Entry name

TF2B_MOUSE

Accession

Primary (citable) accession number: P62915
Secondary accession number(s): P29053

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	August 31, 2004
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program