P62908 (RS3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S3

Gene names

Name:

Rps3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs By similarity.
Subcellular location	Cytoplasm By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs By similarity.
Sequence similarities	Belongs to the ribosomal protein S3P family. Contains 1 KH type-2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara induction of apoptosis Inferred from electronic annotation. Source: Compara negative regulation of DNA repair Inferred from electronic annotation. Source: Compara negative regulation of NF-kappaB transcription factor activity Inferred from electronic annotation. Source: Compara response to DNA damage stimulus Inferred from electronic annotation. Source: Compara translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay PubMed 12388085. Source: MGI cytosolic small ribosomal subunit Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara ribonucleoprotein complex Inferred from sequence or structural similarity. Source: UniProtKB ruffle membrane Inferred from electronic annotation. Source: Compara
Molecular_function	DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: Compara damaged DNA binding Inferred from electronic annotation. Source: Compara endonuclease activity Inferred from electronic annotation. Source: Compara mRNA binding Inferred from electronic annotation. Source: Compara structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 243	242	40S ribosomal protein S3		PRO_0000130321
Regions
	Domain	21 – 92	72	KH type-2
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	62	1	N6-acetyllysine By similarity
	Modified residue	221	1	Phosphothreonine Ref.3 Ref.4
	Modified residue	224	1	Phosphoserine By similarity
	Modified residue	242	1	Phosphothreonine By similarity
	Cross-link	90		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	202		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62908 [UniParc]. Last modified August 31, 2004. Version 1. Checksum: 6B9BB34FDEE04AAF	FASTA	243	26,674
	10 20 30 40 50 60 MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG 70 80 90 100 110 120 EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY 130 140 150 160 170 180 GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG 190 200 210 220 230 240 VLGIKVKIML PWDPSGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary sequence of the mouse ribosomal protein S3." Harper M., Terol-Garay E., Hraba-Renevey S., Kress M.M. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76772 mRNA. Translation: CAA54167.1. BC010721 mRNA. Translation: AAH10721.1.
IPI	IPI00134599.
PIR	S41170.
RefSeq	NP_036182.1. NM_012052.2.
UniGene	Mm.236868.
3D structure databases
ProteinModelPortal	P62908.
SMR	P62908. Positions 2-193.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62908. 6 interactions.
PTM databases
PhosphoSite	P62908.
Proteomic databases
PaxDb	P62908.
PRIDE	P62908.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032998; ENSMUSP00000032998; ENSMUSG00000030744.
GeneID	27050.
KEGG	mmu:27050.
Organism-specific databases
CTD	6188.
MGI	MGI:1350917. Rps3.
Phylogenomic databases
eggNOG	COG0092.
HOGENOM	HOG000210611.
HOVERGEN	HBG002195.
InParanoid	P62908.
KO	K02985.
OMA	CNDYVET.
OrthoDB	EOG4GXFNJ.
Gene expression databases
ArrayExpress	P62908.
Bgee	P62908.
CleanEx	MM_RPS3.
Genevestigator	P62908.
GermOnline	ENSMUSG00000030744. Mus musculus.
Family and domain databases
Gene3D	3.30.1140.32. 1 hit. 3.30.300.20. 1 hit.
InterPro	IPR004087. KH_dom. IPR015946. KH_dom-like_a/b. IPR004044. KH_dom_type_2. IPR009019. KH_prok-type. IPR001351. Ribosomal_S3_C. IPR018280. Ribosomal_S3_CS. IPR005703. Ribosomal_S3_euk/arc. [Graphical view]
Pfam	PF07650. KH_2. 1 hit. PF00189. Ribosomal_S3_C. 1 hit. [Graphical view]
SMART	SM00322. KH. 1 hit. [Graphical view]
SUPFAM	SSF54814. KH_prok. 1 hit. SSF54821. Ribosomal_S3_C. 1 hit.
TIGRFAMs	TIGR01008. rpsC_E_A. 1 hit.
PROSITE	PS50823. KH_TYPE_2. 1 hit. PS00548. RIBOSOMAL_S3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RpS3. mouse.
NextBio	304983.
SOURCE	Search...

Entry information

Entry name

RS3_MOUSE

Accession

Primary (citable) accession number: P62908
Secondary accession number(s): P17073, P47933

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	August 31, 2004
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program