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P62897

- CYC_MOUSE

UniProt

P62897 - CYC_MOUSE

Protein

Cytochrome c, somatic

Gene

Cycs

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.1 Publication
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151Heme (covalent)
    Binding sitei18 – 181Heme (covalent)
    Metal bindingi19 – 191Iron (heme axial ligand)
    Metal bindingi81 – 811Iron (heme axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. oxidation-reduction process Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_189141. Detoxification of Reactive Oxygen Species.
    REACT_205251. Transcriptional activation of mitochondrial biogenesis.
    REACT_206522. Formation of apoptosome.
    REACT_211550. Release of apoptotic factors from the mitochondria.
    REACT_225157. Activation of caspases through apoptosome-mediated cleavage.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c, somatic
    Gene namesi
    Name:Cycs
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:88578. Cycs.

    Subcellular locationi

    Mitochondrion intermembrane space
    Note: Loosely associated with the inner membrane.

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 105104Cytochrome c, somaticPRO_0000108225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycine1 Publication
    Modified residuei49 – 491PhosphotyrosineBy similarity
    Modified residuei98 – 981PhosphotyrosineBy similarity

    Post-translational modificationi

    Binds 1 heme group per subunit.
    Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP62897.
    PaxDbiP62897.
    PRIDEiP62897.

    PTM databases

    PhosphoSiteiP62897.

    Expressioni

    Tissue specificityi

    Found in embryos and in adult liver and heart.

    Gene expression databases

    ArrayExpressiP62897.
    BgeeiP62897.
    CleanExiMM_CYCS.
    GenevestigatoriP62897.

    Interactioni

    Protein-protein interaction databases

    BioGridi198992. 3 interactions.
    583899. 1 interaction.
    IntActiP62897. 6 interactions.
    MINTiMINT-1860070.
    STRINGi10090.ENSMUSP00000076944.

    Structurei

    3D structure databases

    ProteinModelPortaliP62897.
    SMRiP62897. Positions 2-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c family.Curated

    Phylogenomic databases

    eggNOGiCOG3474.
    HOGENOMiHOG000009762.
    HOVERGENiHBG003023.
    InParanoidiP62897.
    KOiK08738.
    OMAiDDQEITD.
    OrthoDBiEOG761BWX.
    PhylomeDBiP62897.
    TreeFamiTF300226.

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view]
    PANTHERiPTHR11961. PTHR11961. 1 hit.
    PfamiPF00034. Cytochrom_C. 1 hit.
    [Graphical view]
    PRINTSiPR00604. CYTCHRMECIAB.
    SUPFAMiSSF46626. SSF46626. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62897-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDVEKGKKI FVQKCAQCHT VEKGGKHKTG PNLHGLFGRK TGQAAGFSYT    50
    DANKNKGITW GEDTLMEYLE NPKKYIPGTK MIFAGIKKKG ERADLIAYLK 100
    KATNE 105
    Length:105
    Mass (Da):11,605
    Last modified:January 23, 2007 - v2
    Checksum:iB5BCA779BCE40492
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01756 Genomic DNA. Translation: CAA25899.1.
    AK088098 mRNA. Translation: BAC40143.1.
    BC034363 mRNA. Translation: AAH34363.1.
    CCDSiCCDS20131.1.
    PIRiA23057. CCMS.
    RefSeqiNP_031834.1. NM_007808.4.
    UniGeneiMm.35389.

    Genome annotation databases

    EnsembliENSMUST00000073080; ENSMUSP00000072829; ENSMUSG00000058927.
    ENSMUST00000161401; ENSMUSP00000124523; ENSMUSG00000063694.
    GeneIDi13063.
    KEGGimmu:13063.
    UCSCiuc009bxc.1. mouse.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Life shuttle - Issue 76 of November 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01756 Genomic DNA. Translation: CAA25899.1 .
    AK088098 mRNA. Translation: BAC40143.1 .
    BC034363 mRNA. Translation: AAH34363.1 .
    CCDSi CCDS20131.1.
    PIRi A23057. CCMS.
    RefSeqi NP_031834.1. NM_007808.4.
    UniGenei Mm.35389.

    3D structure databases

    ProteinModelPortali P62897.
    SMRi P62897. Positions 2-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198992. 3 interactions.
    583899. 1 interaction.
    IntActi P62897. 6 interactions.
    MINTi MINT-1860070.
    STRINGi 10090.ENSMUSP00000076944.

    PTM databases

    PhosphoSitei P62897.

    Proteomic databases

    MaxQBi P62897.
    PaxDbi P62897.
    PRIDEi P62897.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000073080 ; ENSMUSP00000072829 ; ENSMUSG00000058927 .
    ENSMUST00000161401 ; ENSMUSP00000124523 ; ENSMUSG00000063694 .
    GeneIDi 13063.
    KEGGi mmu:13063.
    UCSCi uc009bxc.1. mouse.

    Organism-specific databases

    CTDi 54205.
    MGIi MGI:88578. Cycs.

    Phylogenomic databases

    eggNOGi COG3474.
    HOGENOMi HOG000009762.
    HOVERGENi HBG003023.
    InParanoidi P62897.
    KOi K08738.
    OMAi DDQEITD.
    OrthoDBi EOG761BWX.
    PhylomeDBi P62897.
    TreeFami TF300226.

    Enzyme and pathway databases

    Reactomei REACT_189141. Detoxification of Reactive Oxygen Species.
    REACT_205251. Transcriptional activation of mitochondrial biogenesis.
    REACT_206522. Formation of apoptosome.
    REACT_211550. Release of apoptotic factors from the mitochondria.
    REACT_225157. Activation of caspases through apoptosome-mediated cleavage.

    Miscellaneous databases

    NextBioi 282990.
    PROi P62897.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62897.
    Bgeei P62897.
    CleanExi MM_CYCS.
    Genevestigatori P62897.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view ]
    PANTHERi PTHR11961. PTHR11961. 1 hit.
    Pfami PF00034. Cytochrom_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00604. CYTCHRMECIAB.
    SUPFAMi SSF46626. SSF46626. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a mouse somatic cytochrome c gene and three cytochrome c pseudogenes."
      Limbach K.J., Wu R.
      Nucleic Acids Res. 13:617-630(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
      Tissue: Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
    4. Cited for: PROTEIN SEQUENCE OF 2-105, ACETYLATION AT GLY-2.
      Strain: BALB/c.
    5. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 29-54; 57-74; 81-88 AND 93-100, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Change of cytochrome c structure during development of the mouse."
      Hennig B.
      Eur. J. Biochem. 55:167-183(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: AMINO-ACID COMPOSITION OF TRYPTIC PEPTIDES.
      Strain: BALB/c.
    7. "Nuclear Apaf-1 and cytochrome c redistribution following stress-induced apoptosis."
      Ruiz-Vela A., Gonzalez de Buitrago G., Martinez-A C.
      FEBS Lett. 517:133-138(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN APOPTOSIS.

    Entry informationi

    Entry nameiCYC_MOUSE
    AccessioniPrimary (citable) accession number: P62897
    Secondary accession number(s): P00009, Q8C2S2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3