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Protein

Cytochrome c, somatic

Gene

Cycs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.1 Publication
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151Heme (covalent)
Binding sitei18 – 181Heme (covalent)
Metal bindingi19 – 191Iron (heme axial ligand)
Metal bindingi81 – 811Iron (heme axial ligand)

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. glial cell apoptotic process Source: Ensembl
  2. mitochondrial electron transport, cytochrome c to oxygen Source: Ensembl
  3. negative regulation of hydrogen peroxide biosynthetic process Source: Ensembl
  4. positive regulation of cellular respiration Source: Ensembl
  5. response to ischemia Source: Ensembl
  6. response to oxidative stress Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_272089. Activation of caspases through apoptosome-mediated cleavage.
REACT_278797. Respiratory electron transport.
REACT_297274. Orphan transporters.
REACT_303916. Formation of apoptosome.
REACT_308972. Detoxification of Reactive Oxygen Species.
REACT_311055. Release of apoptotic factors from the mitochondria.
REACT_328033. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c, somatic
Gene namesi
Name:Cycs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componentsi: Chromosome 19, Chromosome 6

Organism-specific databases

MGIiMGI:88578. Cycs.

Subcellular locationi

  1. Mitochondrion intermembrane space

  2. Note: Loosely associated with the inner membrane.

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. mitochondrial intermembrane space Source: UniProtKB-SubCell
  3. myelin sheath Source: UniProtKB
  4. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 105104Cytochrome c, somaticPRO_0000108225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication
Modified residuei49 – 491PhosphotyrosineBy similarity
Modified residuei98 – 981PhosphotyrosineBy similarity

Post-translational modificationi

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP62897.
PaxDbiP62897.
PRIDEiP62897.

PTM databases

PhosphoSiteiP62897.

Expressioni

Tissue specificityi

Found in embryos and in adult liver and heart.

Gene expression databases

BgeeiP62897.
CleanExiMM_CYCS.
ExpressionAtlasiP62897. baseline and differential.
GenevestigatoriP62897.

Interactioni

Protein-protein interaction databases

BioGridi198992. 3 interactions.
583899. 1 interaction.
IntActiP62897. 6 interactions.
MINTiMINT-1860070.
STRINGi10090.ENSMUSP00000076944.

Structurei

3D structure databases

ProteinModelPortaliP62897.
SMRiP62897. Positions 2-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiCOG3474.
HOGENOMiHOG000009762.
HOVERGENiHBG003023.
InParanoidiP62897.
KOiK08738.
OMAiRDYLHNP.
OrthoDBiEOG761BWX.
PhylomeDBiP62897.
TreeFamiTF300226.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDVEKGKKI FVQKCAQCHT VEKGGKHKTG PNLHGLFGRK TGQAAGFSYT
60 70 80 90 100
DANKNKGITW GEDTLMEYLE NPKKYIPGTK MIFAGIKKKG ERADLIAYLK

KATNE
Length:105
Mass (Da):11,605
Last modified:January 23, 2007 - v2
Checksum:iB5BCA779BCE40492
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01756 Genomic DNA. Translation: CAA25899.1.
AK088098 mRNA. Translation: BAC40143.1.
BC034363 mRNA. Translation: AAH34363.1.
CCDSiCCDS20131.1.
PIRiA23057. CCMS.
RefSeqiNP_031834.1. NM_007808.4.
UniGeneiMm.35389.

Genome annotation databases

EnsembliENSMUST00000073080; ENSMUSP00000072829; ENSMUSG00000058927.
ENSMUST00000161401; ENSMUSP00000124523; ENSMUSG00000063694.
GeneIDi13063.
KEGGimmu:13063.
UCSCiuc009bxc.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01756 Genomic DNA. Translation: CAA25899.1.
AK088098 mRNA. Translation: BAC40143.1.
BC034363 mRNA. Translation: AAH34363.1.
CCDSiCCDS20131.1.
PIRiA23057. CCMS.
RefSeqiNP_031834.1. NM_007808.4.
UniGeneiMm.35389.

3D structure databases

ProteinModelPortaliP62897.
SMRiP62897. Positions 2-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198992. 3 interactions.
583899. 1 interaction.
IntActiP62897. 6 interactions.
MINTiMINT-1860070.
STRINGi10090.ENSMUSP00000076944.

PTM databases

PhosphoSiteiP62897.

Proteomic databases

MaxQBiP62897.
PaxDbiP62897.
PRIDEiP62897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073080; ENSMUSP00000072829; ENSMUSG00000058927.
ENSMUST00000161401; ENSMUSP00000124523; ENSMUSG00000063694.
GeneIDi13063.
KEGGimmu:13063.
UCSCiuc009bxc.1. mouse.

Organism-specific databases

CTDi54205.
MGIiMGI:88578. Cycs.

Phylogenomic databases

eggNOGiCOG3474.
HOGENOMiHOG000009762.
HOVERGENiHBG003023.
InParanoidiP62897.
KOiK08738.
OMAiRDYLHNP.
OrthoDBiEOG761BWX.
PhylomeDBiP62897.
TreeFamiTF300226.

Enzyme and pathway databases

ReactomeiREACT_272089. Activation of caspases through apoptosome-mediated cleavage.
REACT_278797. Respiratory electron transport.
REACT_297274. Orphan transporters.
REACT_303916. Formation of apoptosome.
REACT_308972. Detoxification of Reactive Oxygen Species.
REACT_311055. Release of apoptotic factors from the mitochondria.
REACT_328033. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

NextBioi282990.
PROiP62897.
SOURCEiSearch...

Gene expression databases

BgeeiP62897.
CleanExiMM_CYCS.
ExpressionAtlasiP62897. baseline and differential.
GenevestigatoriP62897.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a mouse somatic cytochrome c gene and three cytochrome c pseudogenes."
    Limbach K.J., Wu R.
    Nucleic Acids Res. 13:617-630(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
  4. Cited for: PROTEIN SEQUENCE OF 2-105, ACETYLATION AT GLY-2.
    Strain: BALB/c.
  5. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 29-54; 57-74; 81-88 AND 93-100, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Change of cytochrome c structure during development of the mouse."
    Hennig B.
    Eur. J. Biochem. 55:167-183(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: AMINO-ACID COMPOSITION OF TRYPTIC PEPTIDES.
    Strain: BALB/c.
  7. "Nuclear Apaf-1 and cytochrome c redistribution following stress-induced apoptosis."
    Ruiz-Vela A., Gonzalez de Buitrago G., Martinez-A C.
    FEBS Lett. 517:133-138(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN APOPTOSIS.

Entry informationi

Entry nameiCYC_MOUSE
AccessioniPrimary (citable) accession number: P62897
Secondary accession number(s): P00009, Q8C2S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.