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P62894

- CYC_BOVIN

UniProt

P62894 - CYC_BOVIN

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Protein

Cytochrome c

Gene

CYCS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151Heme (covalent)
Binding sitei18 – 181Heme (covalent)
Metal bindingi19 – 191Iron (heme axial ligand)
Metal bindingi81 – 811Iron (heme axial ligand)

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c
Gene namesi
Name:CYCS
Synonyms:CYC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 4

Subcellular locationi

Mitochondrion intermembrane space
Note: Loosely associated with the inner membrane.

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 105104Cytochrome cPRO_0000108209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication
Modified residuei49 – 491Phosphotyrosine1 Publication
Modified residuei98 – 981Phosphotyrosine1 Publication

Post-translational modificationi

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62894.
PRIDEiP62894.

Interactioni

Protein-protein interaction databases

DIPiDIP-58978N.
IntActiP62894. 1 interaction.
STRINGi9913.ENSBTAP00000051780.

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1411Combined sources
Turni15 – 184Combined sources
Helixi51 – 555Combined sources
Helixi62 – 687Combined sources
Helixi72 – 754Combined sources
Helixi89 – 10214Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B4ZX-ray1.50A2-105[»]
2YBBelectron microscopy19.00Y2-105[»]
3J2Telectron microscopy9.50H/I/J/K/L/M/N2-105[»]
ProteinModelPortaliP62894.
SMRiP62894. Positions 2-105.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62894.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiCOG3474.
GeneTreeiENSGT00390000009405.
HOGENOMiHOG000009762.
HOVERGENiHBG003023.
InParanoidiP62894.
KOiK08738.
OMAiDDQEITD.
OrthoDBiEOG761BWX.
TreeFamiTF300226.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_dom.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62894-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDVEKGKKI FVQKCAQCHT VEKGGKHKTG PNLHGLFGRK TGQAPGFSYT
60 70 80 90 100
DANKNKGITW GEETLMEYLE NPKKYIPGTK MIFAGIKKKG EREDLIAYLK

KATNE
Length:105
Mass (Da):11,704
Last modified:January 23, 2007 - v2
Checksum:iAF0CA628EDF40483
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC105397 mRNA. Translation: AAI05398.1.
PIRiA92022. CCBO.
RefSeqiNP_001039526.1. NM_001046061.2.
XP_005194076.1. XM_005194019.1.
UniGeneiBt.23981.

Genome annotation databases

EnsembliENSBTAT00000004594; ENSBTAP00000051780; ENSBTAG00000023823.
ENSBTAT00000007918; ENSBTAP00000007918; ENSBTAG00000022613.
GeneIDi100850794.
510767.
KEGGibta:100850794.
bta:510767.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC105397 mRNA. Translation: AAI05398.1 .
PIRi A92022. CCBO.
RefSeqi NP_001039526.1. NM_001046061.2.
XP_005194076.1. XM_005194019.1.
UniGenei Bt.23981.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2B4Z X-ray 1.50 A 2-105 [» ]
2YBB electron microscopy 19.00 Y 2-105 [» ]
3J2T electron microscopy 9.50 H/I/J/K/L/M/N 2-105 [» ]
ProteinModelPortali P62894.
SMRi P62894. Positions 2-105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-58978N.
IntActi P62894. 1 interaction.
STRINGi 9913.ENSBTAP00000051780.

Proteomic databases

PaxDbi P62894.
PRIDEi P62894.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000004594 ; ENSBTAP00000051780 ; ENSBTAG00000023823 .
ENSBTAT00000007918 ; ENSBTAP00000007918 ; ENSBTAG00000022613 .
GeneIDi 100850794.
510767.
KEGGi bta:100850794.
bta:510767.

Organism-specific databases

CTDi 54205.

Phylogenomic databases

eggNOGi COG3474.
GeneTreei ENSGT00390000009405.
HOGENOMi HOG000009762.
HOVERGENi HBG003023.
InParanoidi P62894.
KOi K08738.
OMAi DDQEITD.
OrthoDBi EOG761BWX.
TreeFami TF300226.

Miscellaneous databases

EvolutionaryTracei P62894.
NextBioi 20869605.

Family and domain databases

Gene3Di 1.10.760.10. 1 hit.
InterProi IPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_dom.
[Graphical view ]
PANTHERi PTHR11961. PTHR11961. 1 hit.
Pfami PF00034. Cytochrom_C. 1 hit.
[Graphical view ]
PRINTSi PR00604. CYTCHRMECIAB.
SUPFAMi SSF46626. SSF46626. 1 hit.
PROSITEi PS51007. CYTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.
  2. "The amino acid sequence of bovine heart cytochrome c."
    Nakashima T., Higa H., Matsubara H., Benson A.M., Yasunobu K.T.
    J. Biol. Chem. 241:1166-1177(1966) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-105, ACETYLATION AT GLY-2.
    Tissue: Heart.
  3. "New prospects for an old enzyme: mammalian cytochrome c is tyrosine-phosphorylated in vivo."
    Lee I., Salomon A.R., Yu K., Doan J.W., Grossman L.I., Huttemann M.
    Biochemistry 45:9121-9128(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-49.
    Tissue: Heart.
  4. "Mammalian liver cytochrome c is tyrosine-48 phosphorylated in vivo, inhibiting mitochondrial respiration."
    Yu H., Lee I., Salomon A.R., Yu K., Huttemann M.
    Biochim. Biophys. Acta 1777:1066-1071(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-98.
    Tissue: Liver.
  5. "High resolution X-ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor."
    Mirkin N., Jaconcic J., Stojanoff V., Moreno A.
    Proteins 70:83-92(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-105, HEME-BINDING SITES.

Entry informationi

Entry nameiCYC_BOVIN
AccessioniPrimary (citable) accession number: P62894
Secondary accession number(s): P00006, Q2KJD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3