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P62894

- CYC_BOVIN

UniProt

P62894 - CYC_BOVIN

Protein

Cytochrome c

Gene

CYCS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151Heme (covalent)
    Binding sitei18 – 181Heme (covalent)
    Metal bindingi19 – 191Iron (heme axial ligand)
    Metal bindingi81 – 811Iron (heme axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. oxidation-reduction process Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c
    Gene namesi
    Name:CYCS
    Synonyms:CYC
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 4

    Subcellular locationi

    Mitochondrion intermembrane space
    Note: Loosely associated with the inner membrane.

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 105104Cytochrome cPRO_0000108209Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycine1 Publication
    Modified residuei49 – 491Phosphotyrosine1 Publication
    Modified residuei98 – 981Phosphotyrosine1 Publication

    Post-translational modificationi

    Binds 1 heme group per subunit.
    Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP62894.
    PRIDEiP62894.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-58978N.
    IntActiP62894. 1 interaction.
    STRINGi9913.ENSBTAP00000051780.

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1411
    Turni15 – 184
    Helixi51 – 555
    Helixi62 – 687
    Helixi72 – 754
    Helixi89 – 10214

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2B4ZX-ray1.50A2-105[»]
    2YBBelectron microscopy19.00Y2-105[»]
    3J2Telectron microscopy9.50H/I/J/K/L/M/N2-105[»]
    ProteinModelPortaliP62894.
    SMRiP62894. Positions 2-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62894.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c family.Curated

    Phylogenomic databases

    eggNOGiCOG3474.
    GeneTreeiENSGT00390000009405.
    HOGENOMiHOG000009762.
    HOVERGENiHBG003023.
    InParanoidiP62894.
    KOiK08738.
    OMAiDDQEITD.
    OrthoDBiEOG761BWX.
    TreeFamiTF300226.

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view]
    PANTHERiPTHR11961. PTHR11961. 1 hit.
    PfamiPF00034. Cytochrom_C. 1 hit.
    [Graphical view]
    PRINTSiPR00604. CYTCHRMECIAB.
    SUPFAMiSSF46626. SSF46626. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62894-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDVEKGKKI FVQKCAQCHT VEKGGKHKTG PNLHGLFGRK TGQAPGFSYT    50
    DANKNKGITW GEETLMEYLE NPKKYIPGTK MIFAGIKKKG EREDLIAYLK 100
    KATNE 105
    Length:105
    Mass (Da):11,704
    Last modified:January 23, 2007 - v2
    Checksum:iAF0CA628EDF40483
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC105397 mRNA. Translation: AAI05398.1.
    PIRiA92022. CCBO.
    RefSeqiNP_001039526.1. NM_001046061.2.
    XP_005194076.1. XM_005194019.1.
    UniGeneiBt.23981.

    Genome annotation databases

    EnsembliENSBTAT00000004594; ENSBTAP00000051780; ENSBTAG00000023823.
    ENSBTAT00000007918; ENSBTAP00000007918; ENSBTAG00000022613.
    GeneIDi100850794.
    510767.
    KEGGibta:100850794.
    bta:510767.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Life shuttle - Issue 76 of November 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC105397 mRNA. Translation: AAI05398.1 .
    PIRi A92022. CCBO.
    RefSeqi NP_001039526.1. NM_001046061.2.
    XP_005194076.1. XM_005194019.1.
    UniGenei Bt.23981.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2B4Z X-ray 1.50 A 2-105 [» ]
    2YBB electron microscopy 19.00 Y 2-105 [» ]
    3J2T electron microscopy 9.50 H/I/J/K/L/M/N 2-105 [» ]
    ProteinModelPortali P62894.
    SMRi P62894. Positions 2-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-58978N.
    IntActi P62894. 1 interaction.
    STRINGi 9913.ENSBTAP00000051780.

    Proteomic databases

    PaxDbi P62894.
    PRIDEi P62894.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004594 ; ENSBTAP00000051780 ; ENSBTAG00000023823 .
    ENSBTAT00000007918 ; ENSBTAP00000007918 ; ENSBTAG00000022613 .
    GeneIDi 100850794.
    510767.
    KEGGi bta:100850794.
    bta:510767.

    Organism-specific databases

    CTDi 54205.

    Phylogenomic databases

    eggNOGi COG3474.
    GeneTreei ENSGT00390000009405.
    HOGENOMi HOG000009762.
    HOVERGENi HBG003023.
    InParanoidi P62894.
    KOi K08738.
    OMAi DDQEITD.
    OrthoDBi EOG761BWX.
    TreeFami TF300226.

    Miscellaneous databases

    EvolutionaryTracei P62894.
    NextBioi 20869605.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view ]
    PANTHERi PTHR11961. PTHR11961. 1 hit.
    Pfami PF00034. Cytochrom_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00604. CYTCHRMECIAB.
    SUPFAMi SSF46626. SSF46626. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Thymus.
    2. "The amino acid sequence of bovine heart cytochrome c."
      Nakashima T., Higa H., Matsubara H., Benson A.M., Yasunobu K.T.
      J. Biol. Chem. 241:1166-1177(1966) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-105, ACETYLATION AT GLY-2.
      Tissue: Heart.
    3. "New prospects for an old enzyme: mammalian cytochrome c is tyrosine-phosphorylated in vivo."
      Lee I., Salomon A.R., Yu K., Doan J.W., Grossman L.I., Huttemann M.
      Biochemistry 45:9121-9128(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-49.
      Tissue: Heart.
    4. "Mammalian liver cytochrome c is tyrosine-48 phosphorylated in vivo, inhibiting mitochondrial respiration."
      Yu H., Lee I., Salomon A.R., Yu K., Huttemann M.
      Biochim. Biophys. Acta 1777:1066-1071(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-98.
      Tissue: Liver.
    5. "High resolution X-ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor."
      Mirkin N., Jaconcic J., Stojanoff V., Moreno A.
      Proteins 70:83-92(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-105, HEME-BINDING SITES.

    Entry informationi

    Entry nameiCYC_BOVIN
    AccessioniPrimary (citable) accession number: P62894
    Secondary accession number(s): P00006, Q2KJD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3