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Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

Gnb5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.Curated

GO - Molecular functioni

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of voltage-gated calcium channel activity Source: UniProtKB
  • signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene namesi
Name:Gnb5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620759. Gnb5.

Subcellular locationi

  • Membrane By similarity

GO - Cellular componenti

  • cell body Source: RGD
  • cytosol Source: RGD
  • heterotrimeric G-protein complex Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Guanine nucleotide-binding protein subunit beta-5PRO_0000127709Add
BLAST

Proteomic databases

PRIDEiP62882.

PTM databases

PhosphoSiteiP62882.

Expressioni

Tissue specificityi

Detected in brain.1 Publication

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 subunits; alpha, beta and gamma. Interacts with RGS9. Component of a complex composed of RGS9 (isoform RGS9-1), GNB5 and RGS9BP (By similarity). Interacts with RGS6 and RGS7 (By similarity).By similarity

GO - Molecular functioni

  • chaperone binding Source: UniProtKB
  • G-protein gamma-subunit binding Source: UniProtKB
  • GTPase activating protein binding Source: RGD

Protein-protein interaction databases

BioGridi249765. 1 interaction.
IntActiP62882. 2 interactions.
MINTiMINT-7720278.

Structurei

3D structure databases

ProteinModelPortaliP62882.
SMRiP62882. Positions 1-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati61 – 10040WD 1Add
BLAST
Repeati103 – 14240WD 2Add
BLAST
Repeati151 – 19242WD 3Add
BLAST
Repeati194 – 23643WD 4Add
BLAST
Repeati237 – 27640WD 5Add
BLAST
Repeati278 – 32043WD 6Add
BLAST
Repeati323 – 35230WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOVERGENiHBG000188.
InParanoidiP62882.
KOiK04539.
PhylomeDBiP62882.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62882-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDGLHENE TLASLKSEAE SLKGKLEEER AKLHDVELHQ VAERVEALGQ
60 70 80 90 100
FVMKTRRTLK GHGNKVLCMD WCKDKRRIVS SSQDGKVIVW DSFTTNKEHA
110 120 130 140 150
VTMPCTWVMA CAYAPSGCAI ACGGLDNKCS VYPLTFDKNE NMAAKKKSVA
160 170 180 190 200
MHTNYLSACS FTNSDMQILT ASGDGTCALW DVESGQLLQS FHGHGADVLC
210 220 230 240 250
LDLAPSETGN TFVSGGCDKK AMVWDMRSGQ CVQAFETHES DVNSVRYYPS
260 270 280 290 300
GDAFASGSDD ATCRLYDLRA DREVAIYSKE SIIFGASSVD FSLSGRLLFA
310 320 330 340 350
GYNDYTINVW DVLKGSRVSI LFGHENRVST LRVSPDGTAF CSGSWDHTLR

VWA
Length:353
Mass (Da):38,732
Last modified:August 16, 2004 - v1
Checksum:i30FCF51C125A024D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001953 mRNA. Translation: AAB59974.1.
AY552803 mRNA. Translation: AAS59141.1.
BC089221 mRNA. Translation: AAH89221.1.
AF022086 mRNA. Translation: AAB82553.1.
RefSeqiNP_113958.1. NM_031770.3.
UniGeneiRn.23042.

Genome annotation databases

GeneIDi83579.
KEGGirno:83579.
UCSCiRGD:620759. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001953 mRNA. Translation: AAB59974.1.
AY552803 mRNA. Translation: AAS59141.1.
BC089221 mRNA. Translation: AAH89221.1.
AF022086 mRNA. Translation: AAB82553.1.
RefSeqiNP_113958.1. NM_031770.3.
UniGeneiRn.23042.

3D structure databases

ProteinModelPortaliP62882.
SMRiP62882. Positions 1-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249765. 1 interaction.
IntActiP62882. 2 interactions.
MINTiMINT-7720278.

PTM databases

PhosphoSiteiP62882.

Proteomic databases

PRIDEiP62882.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83579.
KEGGirno:83579.
UCSCiRGD:620759. rat.

Organism-specific databases

CTDi10681.
RGDi620759. Gnb5.

Phylogenomic databases

HOVERGENiHBG000188.
InParanoidiP62882.
KOiK04539.
PhylomeDBiP62882.

Miscellaneous databases

PROiP62882.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Levay K., Slepak V.Z.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Cloning and characterization of the rat G-protein beta 5 subunit."
    Puhl H.L. III, Ikeda S.R.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain."
    Betty M., Harnish S.W., Rhodes K.J., Cockett M.I.
    Neuroscience 85:475-486(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-198, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiGBB5_RAT
AccessioniPrimary (citable) accession number: P62882
Secondary accession number(s): O35354
, P54314, Q5FWS8, Q91WB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.