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P62881

- GBB5_MOUSE

UniProt

P62881 - GBB5_MOUSE

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Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

Gnb5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  1. chaperone binding Source: UniProt
  2. G-protein gamma-subunit binding Source: UniProt
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: MGI
  2. negative regulation of voltage-gated calcium channel activity Source: UniProt
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiREACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_213947. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_231304. Glucagon-type ligand receptors.
REACT_248547. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_250590. Adrenaline,noradrenaline inhibits insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene namesi
Name:Gnb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:101848. Gnb5.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
  2. plasma membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Guanine nucleotide-binding protein subunit beta-5PRO_0000127706Add
BLAST

Proteomic databases

MaxQBiP62881.
PaxDbiP62881.
PRIDEiP62881.

PTM databases

PhosphoSiteiP62881.

Expressioni

Tissue specificityi

Brain and at much reduced levels in kidney. Isoform 1 is retinal specific.1 Publication

Gene expression databases

BgeeiP62881.
ExpressionAtlasiP62881. baseline and differential.
GenevestigatoriP62881.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP.

Protein-protein interaction databases

BioGridi199981. 1 interaction.
DIPiDIP-264N.
IntActiP62881. 3 interactions.
MINTiMINT-4095704.

Structurei

Secondary structure

1
395
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi52 – 7322Combined sources
Helixi80 – 834Combined sources
Turni84 – 863Combined sources
Beta strandi97 – 1026Combined sources
Beta strandi108 – 1136Combined sources
Beta strandi117 – 1248Combined sources
Beta strandi127 – 1337Combined sources
Turni134 – 1363Combined sources
Beta strandi139 – 1446Combined sources
Beta strandi146 – 1483Combined sources
Beta strandi152 – 1554Combined sources
Beta strandi159 – 1668Combined sources
Beta strandi169 – 1757Combined sources
Helixi185 – 1873Combined sources
Beta strandi189 – 1935Combined sources
Beta strandi198 – 2036Combined sources
Beta strandi205 – 21410Combined sources
Beta strandi217 – 2237Combined sources
Turni224 – 2263Combined sources
Beta strandi229 – 2346Combined sources
Beta strandi240 – 2456Combined sources
Beta strandi253 – 2586Combined sources
Beta strandi263 – 2675Combined sources
Turni268 – 2703Combined sources
Beta strandi273 – 2775Combined sources
Beta strandi284 – 2896Combined sources
Beta strandi293 – 3008Combined sources
Beta strandi305 – 3095Combined sources
Turni310 – 3134Combined sources
Beta strandi314 – 3196Combined sources
Beta strandi328 – 3336Combined sources
Beta strandi337 – 3448Combined sources
Beta strandi349 – 3535Combined sources
Turni354 – 3563Combined sources
Beta strandi358 – 3636Combined sources
Beta strandi370 – 3756Combined sources
Beta strandi382 – 3865Combined sources
Beta strandi389 – 3946Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95B/D43-395[»]
ProteinModelPortaliP62881.
SMRiP62881. Positions 43-395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62881.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati103 – 14240WD 1Add
BLAST
Repeati145 – 18440WD 2Add
BLAST
Repeati193 – 23442WD 3Add
BLAST
Repeati236 – 27843WD 4Add
BLAST
Repeati279 – 31840WD 5Add
BLAST
Repeati320 – 36243WD 6Add
BLAST
Repeati365 – 39430WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62881.
KOiK04539.
OMAiDQTFLVN.
OrthoDBiEOG7GN2N5.
PhylomeDBiP62881.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P62881-1) [UniParc]FASTAAdd to Basket

Also known as: Long, Beta-5L, Gbeta5L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE
60 70 80 90 100
NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT
110 120 130 140 150
LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV
160 170 180 190 200
MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA
210 220 230 240 250
CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET
260 270 280 290 300
GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS
310 320 330 340 350
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN
360 370 380 390
VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA
Length:395
Mass (Da):43,565
Last modified:August 16, 2004 - v1
Checksum:i972A41AAEC4CB3F8
GO
Isoform 2 (identifier: P62881-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:353
Mass (Da):38,732
Checksum:i30FCF51C125A024D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4242Missing in isoform 2. 1 PublicationVSP_008766Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34290 mRNA. Translation: AAA93084.1.
U69145 mRNA. Translation: AAC52886.1.
BC016135 mRNA. Translation: AAH16135.1.
CCDSiCCDS40692.1.
PIRiA54969.
RefSeqiNP_034443.1. NM_010313.2. [P62881-1]
NP_619733.1. NM_138719.5. [P62881-2]
UniGeneiMm.17604.

Genome annotation databases

EnsembliENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192. [P62881-1]
GeneIDi14697.
KEGGimmu:14697.
UCSCiuc009qry.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34290 mRNA. Translation: AAA93084.1 .
U69145 mRNA. Translation: AAC52886.1 .
BC016135 mRNA. Translation: AAH16135.1 .
CCDSi CCDS40692.1.
PIRi A54969.
RefSeqi NP_034443.1. NM_010313.2. [P62881-1 ]
NP_619733.1. NM_138719.5. [P62881-2 ]
UniGenei Mm.17604.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PBI X-ray 1.95 B/D 43-395 [» ]
ProteinModelPortali P62881.
SMRi P62881. Positions 43-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199981. 1 interaction.
DIPi DIP-264N.
IntActi P62881. 3 interactions.
MINTi MINT-4095704.

PTM databases

PhosphoSitei P62881.

Proteomic databases

MaxQBi P62881.
PaxDbi P62881.
PRIDEi P62881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000076889 ; ENSMUSP00000076155 ; ENSMUSG00000032192 . [P62881-1 ]
GeneIDi 14697.
KEGGi mmu:14697.
UCSCi uc009qry.1. mouse.

Organism-specific databases

CTDi 10681.
MGIi MGI:101848. Gnb5.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00760000119239.
HOGENOMi HOG000176356.
HOVERGENi HBG000188.
InParanoidi P62881.
KOi K04539.
OMAi DQTFLVN.
OrthoDBi EOG7GN2N5.
PhylomeDBi P62881.
TreeFami TF106149.

Enzyme and pathway databases

Reactomei REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_213947. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_231304. Glucagon-type ligand receptors.
REACT_248547. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_250590. Adrenaline,noradrenaline inhibits insulin secretion.

Miscellaneous databases

ChiTaRSi Gnb5. mouse.
EvolutionaryTracei P62881.
NextBioi 286651.
PROi P62881.
SOURCEi Search...

Gene expression databases

Bgeei P62881.
ExpressionAtlasi P62881. baseline and differential.
Genevestigatori P62881.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 7 hits.
[Graphical view ]
PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
PRINTSi PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C."
    Watson A.J., Katz A., Simon M.I.
    J. Biol. Chem. 269:22150-22156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina."
    Watson A.J., Aragay A.M., Slepak V.Z., Simon M.I.
    J. Biol. Chem. 271:28154-28160(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Retina.
  4. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 75-86 AND 322-338, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.
  5. "R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1."
    Hu G., Wensel T.G.
    Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RGS9 AND RGS9BP.
    Strain: C57BL/6 X 129.
    Tissue: Retina.
  6. Cited for: INTERACTION WITH RGS9 AND RGS9BP.

Entry informationi

Entry nameiGBB5_MOUSE
AccessioniPrimary (citable) accession number: P62881
Secondary accession number(s): O35354, P54314, Q91WB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 26, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3