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P62881

- GBB5_MOUSE

UniProt

P62881 - GBB5_MOUSE

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Protein
Guanine nucleotide-binding protein subunit beta-5
Gene
Gnb5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  1. G-protein gamma-subunit binding Source: UniProt
  2. chaperone binding Source: UniProt
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: MGI
  2. negative regulation of voltage-gated calcium channel activity Source: UniProt
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiREACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_213947. Regulation of water balance by renal Aquaporins.
REACT_221970. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene namesi
Name:Gnb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:101848. Gnb5.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
  2. plasma membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Guanine nucleotide-binding protein subunit beta-5
PRO_0000127706Add
BLAST

Proteomic databases

MaxQBiP62881.
PaxDbiP62881.
PRIDEiP62881.

PTM databases

PhosphoSiteiP62881.

Expressioni

Tissue specificityi

Brain and at much reduced levels in kidney. Isoform 1 is retinal specific.1 Publication

Gene expression databases

ArrayExpressiP62881.
BgeeiP62881.
GenevestigatoriP62881.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP.

Protein-protein interaction databases

BioGridi199981. 1 interaction.
DIPiDIP-264N.
IntActiP62881. 3 interactions.
MINTiMINT-4095704.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi52 – 7322
Helixi80 – 834
Turni84 – 863
Beta strandi97 – 1026
Beta strandi108 – 1136
Beta strandi117 – 1248
Beta strandi127 – 1337
Turni134 – 1363
Beta strandi139 – 1446
Beta strandi146 – 1483
Beta strandi152 – 1554
Beta strandi159 – 1668
Beta strandi169 – 1757
Helixi185 – 1873
Beta strandi189 – 1935
Beta strandi198 – 2036
Beta strandi205 – 21410
Beta strandi217 – 2237
Turni224 – 2263
Beta strandi229 – 2346
Beta strandi240 – 2456
Beta strandi253 – 2586
Beta strandi263 – 2675
Turni268 – 2703
Beta strandi273 – 2775
Beta strandi284 – 2896
Beta strandi293 – 3008
Beta strandi305 – 3095
Turni310 – 3134
Beta strandi314 – 3196
Beta strandi328 – 3336
Beta strandi337 – 3448
Beta strandi349 – 3535
Turni354 – 3563
Beta strandi358 – 3636
Beta strandi370 – 3756
Beta strandi382 – 3865
Beta strandi389 – 3946

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95B/D43-395[»]
ProteinModelPortaliP62881.
SMRiP62881. Positions 43-395.

Miscellaneous databases

EvolutionaryTraceiP62881.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati103 – 14240WD 1
Add
BLAST
Repeati145 – 18440WD 2
Add
BLAST
Repeati193 – 23442WD 3
Add
BLAST
Repeati236 – 27843WD 4
Add
BLAST
Repeati279 – 31840WD 5
Add
BLAST
Repeati320 – 36243WD 6
Add
BLAST
Repeati365 – 39430WD 7
Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00740000115438.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62881.
KOiK04539.
OMAiDQTFLVN.
OrthoDBiEOG7GN2N5.
PhylomeDBiP62881.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P62881-1) [UniParc]FASTAAdd to Basket

Also known as: Long, Beta-5L, Gbeta5L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE    50
NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT 100
LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV 150
MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA 200
CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET 250
GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS 300
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN 350
VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA 395
Length:395
Mass (Da):43,565
Last modified:August 16, 2004 - v1
Checksum:i972A41AAEC4CB3F8
GO
Isoform 2 (identifier: P62881-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:353
Mass (Da):38,732
Checksum:i30FCF51C125A024D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4242Missing in isoform 2.
VSP_008766Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34290 mRNA. Translation: AAA93084.1.
U69145 mRNA. Translation: AAC52886.1.
BC016135 mRNA. Translation: AAH16135.1.
CCDSiCCDS40692.1.
PIRiA54969.
RefSeqiNP_034443.1. NM_010313.2. [P62881-1]
NP_619733.1. NM_138719.5. [P62881-2]
UniGeneiMm.17604.

Genome annotation databases

EnsembliENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192. [P62881-1]
GeneIDi14697.
KEGGimmu:14697.
UCSCiuc009qry.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34290 mRNA. Translation: AAA93084.1 .
U69145 mRNA. Translation: AAC52886.1 .
BC016135 mRNA. Translation: AAH16135.1 .
CCDSi CCDS40692.1.
PIRi A54969.
RefSeqi NP_034443.1. NM_010313.2. [P62881-1 ]
NP_619733.1. NM_138719.5. [P62881-2 ]
UniGenei Mm.17604.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PBI X-ray 1.95 B/D 43-395 [» ]
ProteinModelPortali P62881.
SMRi P62881. Positions 43-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199981. 1 interaction.
DIPi DIP-264N.
IntActi P62881. 3 interactions.
MINTi MINT-4095704.

PTM databases

PhosphoSitei P62881.

Proteomic databases

MaxQBi P62881.
PaxDbi P62881.
PRIDEi P62881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000076889 ; ENSMUSP00000076155 ; ENSMUSG00000032192 . [P62881-1 ]
GeneIDi 14697.
KEGGi mmu:14697.
UCSCi uc009qry.1. mouse.

Organism-specific databases

CTDi 10681.
MGIi MGI:101848. Gnb5.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00740000115438.
HOGENOMi HOG000176356.
HOVERGENi HBG000188.
InParanoidi P62881.
KOi K04539.
OMAi DQTFLVN.
OrthoDBi EOG7GN2N5.
PhylomeDBi P62881.
TreeFami TF106149.

Enzyme and pathway databases

Reactomei REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_213947. Regulation of water balance by renal Aquaporins.
REACT_221970. Ca2+ pathway.

Miscellaneous databases

EvolutionaryTracei P62881.
NextBioi 286651.
PROi P62881.
SOURCEi Search...

Gene expression databases

ArrayExpressi P62881.
Bgeei P62881.
Genevestigatori P62881.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 7 hits.
[Graphical view ]
PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
PRINTSi PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C."
    Watson A.J., Katz A., Simon M.I.
    J. Biol. Chem. 269:22150-22156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina."
    Watson A.J., Aragay A.M., Slepak V.Z., Simon M.I.
    J. Biol. Chem. 271:28154-28160(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Retina.
  4. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 75-86 AND 322-338, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.
  5. "R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1."
    Hu G., Wensel T.G.
    Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RGS9 AND RGS9BP.
    Strain: C57BL/6 X 129.
    Tissue: Retina.
  6. Cited for: INTERACTION WITH RGS9 AND RGS9BP.

Entry informationi

Entry nameiGBB5_MOUSE
AccessioniPrimary (citable) accession number: P62881
Secondary accession number(s): O35354, P54314, Q91WB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi