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P62881

- GBB5_MOUSE

UniProt

P62881 - GBB5_MOUSE

Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

Gnb5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. chaperone binding Source: UniProt
    2. G-protein gamma-subunit binding Source: UniProt
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. G-protein coupled receptor signaling pathway Source: MGI
    2. negative regulation of voltage-gated calcium channel activity Source: UniProt

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_213947. Regulation of water balance by renal Aquaporins.
    REACT_221970. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein subunit beta-5
    Alternative name(s):
    Gbeta5
    Transducin beta chain 5
    Gene namesi
    Name:Gnb5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:101848. Gnb5.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI
    2. plasma membrane Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Guanine nucleotide-binding protein subunit beta-5PRO_0000127706Add
    BLAST

    Proteomic databases

    MaxQBiP62881.
    PaxDbiP62881.
    PRIDEiP62881.

    PTM databases

    PhosphoSiteiP62881.

    Expressioni

    Tissue specificityi

    Brain and at much reduced levels in kidney. Isoform 1 is retinal specific.1 Publication

    Gene expression databases

    ArrayExpressiP62881.
    BgeeiP62881.
    GenevestigatoriP62881.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP.

    Protein-protein interaction databases

    BioGridi199981. 1 interaction.
    DIPiDIP-264N.
    IntActiP62881. 3 interactions.
    MINTiMINT-4095704.

    Structurei

    Secondary structure

    1
    395
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi52 – 7322
    Helixi80 – 834
    Turni84 – 863
    Beta strandi97 – 1026
    Beta strandi108 – 1136
    Beta strandi117 – 1248
    Beta strandi127 – 1337
    Turni134 – 1363
    Beta strandi139 – 1446
    Beta strandi146 – 1483
    Beta strandi152 – 1554
    Beta strandi159 – 1668
    Beta strandi169 – 1757
    Helixi185 – 1873
    Beta strandi189 – 1935
    Beta strandi198 – 2036
    Beta strandi205 – 21410
    Beta strandi217 – 2237
    Turni224 – 2263
    Beta strandi229 – 2346
    Beta strandi240 – 2456
    Beta strandi253 – 2586
    Beta strandi263 – 2675
    Turni268 – 2703
    Beta strandi273 – 2775
    Beta strandi284 – 2896
    Beta strandi293 – 3008
    Beta strandi305 – 3095
    Turni310 – 3134
    Beta strandi314 – 3196
    Beta strandi328 – 3336
    Beta strandi337 – 3448
    Beta strandi349 – 3535
    Turni354 – 3563
    Beta strandi358 – 3636
    Beta strandi370 – 3756
    Beta strandi382 – 3865
    Beta strandi389 – 3946

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PBIX-ray1.95B/D43-395[»]
    ProteinModelPortaliP62881.
    SMRiP62881. Positions 43-395.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62881.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati103 – 14240WD 1Add
    BLAST
    Repeati145 – 18440WD 2Add
    BLAST
    Repeati193 – 23442WD 3Add
    BLAST
    Repeati236 – 27843WD 4Add
    BLAST
    Repeati279 – 31840WD 5Add
    BLAST
    Repeati320 – 36243WD 6Add
    BLAST
    Repeati365 – 39430WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115438.
    HOGENOMiHOG000176356.
    HOVERGENiHBG000188.
    InParanoidiP62881.
    KOiK04539.
    OMAiDQTFLVN.
    OrthoDBiEOG7GN2N5.
    PhylomeDBiP62881.
    TreeFamiTF106149.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P62881-1) [UniParc]FASTAAdd to Basket

    Also known as: Long, Beta-5L, Gbeta5L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE    50
    NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT 100
    LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV 150
    MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA 200
    CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET 250
    GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS 300
    DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN 350
    VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA 395
    Length:395
    Mass (Da):43,565
    Last modified:August 16, 2004 - v1
    Checksum:i972A41AAEC4CB3F8
    GO
    Isoform 2 (identifier: P62881-2) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-42: Missing.

    Show »
    Length:353
    Mass (Da):38,732
    Checksum:i30FCF51C125A024D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4242Missing in isoform 2. 1 PublicationVSP_008766Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34290 mRNA. Translation: AAA93084.1.
    U69145 mRNA. Translation: AAC52886.1.
    BC016135 mRNA. Translation: AAH16135.1.
    CCDSiCCDS40692.1.
    PIRiA54969.
    RefSeqiNP_034443.1. NM_010313.2. [P62881-1]
    NP_619733.1. NM_138719.5. [P62881-2]
    UniGeneiMm.17604.

    Genome annotation databases

    EnsembliENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192. [P62881-1]
    GeneIDi14697.
    KEGGimmu:14697.
    UCSCiuc009qry.1. mouse.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34290 mRNA. Translation: AAA93084.1 .
    U69145 mRNA. Translation: AAC52886.1 .
    BC016135 mRNA. Translation: AAH16135.1 .
    CCDSi CCDS40692.1.
    PIRi A54969.
    RefSeqi NP_034443.1. NM_010313.2. [P62881-1 ]
    NP_619733.1. NM_138719.5. [P62881-2 ]
    UniGenei Mm.17604.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PBI X-ray 1.95 B/D 43-395 [» ]
    ProteinModelPortali P62881.
    SMRi P62881. Positions 43-395.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199981. 1 interaction.
    DIPi DIP-264N.
    IntActi P62881. 3 interactions.
    MINTi MINT-4095704.

    PTM databases

    PhosphoSitei P62881.

    Proteomic databases

    MaxQBi P62881.
    PaxDbi P62881.
    PRIDEi P62881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000076889 ; ENSMUSP00000076155 ; ENSMUSG00000032192 . [P62881-1 ]
    GeneIDi 14697.
    KEGGi mmu:14697.
    UCSCi uc009qry.1. mouse.

    Organism-specific databases

    CTDi 10681.
    MGIi MGI:101848. Gnb5.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115438.
    HOGENOMi HOG000176356.
    HOVERGENi HBG000188.
    InParanoidi P62881.
    KOi K04539.
    OMAi DQTFLVN.
    OrthoDBi EOG7GN2N5.
    PhylomeDBi P62881.
    TreeFami TF106149.

    Enzyme and pathway databases

    Reactomei REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_213947. Regulation of water balance by renal Aquaporins.
    REACT_221970. Ca2+ pathway.

    Miscellaneous databases

    EvolutionaryTracei P62881.
    NextBioi 286651.
    PROi P62881.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62881.
    Bgeei P62881.
    Genevestigatori P62881.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C."
      Watson A.J., Katz A., Simon M.I.
      J. Biol. Chem. 269:22150-22156(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina."
      Watson A.J., Aragay A.M., Slepak V.Z., Simon M.I.
      J. Biol. Chem. 271:28154-28160(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye and Retina.
    4. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 75-86 AND 322-338, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.
    5. "R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1."
      Hu G., Wensel T.G.
      Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RGS9 AND RGS9BP.
      Strain: C57BL/6 X 129.
      Tissue: Retina.
    6. Cited for: INTERACTION WITH RGS9 AND RGS9BP.

    Entry informationi

    Entry nameiGBB5_MOUSE
    AccessioniPrimary (citable) accession number: P62881
    Secondary accession number(s): O35354, P54314, Q91WB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3