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Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

Gnb5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D2 dopamine receptors (By similarity). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).By similarityCurated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer

Enzyme and pathway databases

ReactomeiR-MMU-202040 G-protein activation
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-392451 G beta:gamma signalling through PI3Kgamma
R-MMU-392851 Prostacyclin signalling through prostacyclin receptor
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-4086398 Ca2+ pathway
R-MMU-416476 G alpha (q) signalling events
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-418217 G beta:gamma signalling through PLC beta
R-MMU-418555 G alpha (s) signalling events
R-MMU-418592 ADP signalling through P2Y purinoceptor 1
R-MMU-418594 G alpha (i) signalling events
R-MMU-418597 G alpha (z) signalling events
R-MMU-420092 Glucagon-type ligand receptors
R-MMU-428930 Thromboxane signalling through TP receptor
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-MMU-500657 Presynaptic function of Kainate receptors
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene namesi
Name:Gnb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:101848 Gnb5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277061 – 395Guanine nucleotide-binding protein subunit beta-5Add BLAST395

Proteomic databases

MaxQBiP62881
PaxDbiP62881
PeptideAtlasiP62881
PRIDEiP62881

PTM databases

iPTMnetiP62881
PhosphoSitePlusiP62881

Expressioni

Tissue specificityi

Isoform 1 is only detected in retina (PubMed:8910430). Isoform 2 is detected in brain (at protein level) (PubMed:8071339). Isoform 2 is detected in brain (PubMed:8071339).2 Publications

Gene expression databases

BgeeiENSMUSG00000032192
ExpressionAtlasiP62881 baseline and differential
GenevisibleiP62881 MM

Interactioni

Subunit structurei

Component of a complex composed of RGS9, GNB5 and RGS9BP; within this complex, the presence of GNB5 stabilizes both itself and RGS9 and increases RGS9 GTPase-activating protein (GAP) activity (By similarity) (PubMed:12119397, PubMed:16908407, PubMed:18204463). Interacts with RGS6 and RGS7 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1999811 interactor.
CORUMiP62881
DIPiDIP-264N
IntActiP62881 4 interactors.
MINTiP62881
STRINGi10090.ENSMUSP00000076155

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi52 – 73Combined sources22
Helixi80 – 83Combined sources4
Turni84 – 86Combined sources3
Beta strandi97 – 102Combined sources6
Beta strandi108 – 113Combined sources6
Beta strandi117 – 124Combined sources8
Beta strandi127 – 133Combined sources7
Turni134 – 136Combined sources3
Beta strandi139 – 144Combined sources6
Beta strandi146 – 148Combined sources3
Beta strandi152 – 155Combined sources4
Beta strandi159 – 166Combined sources8
Beta strandi169 – 175Combined sources7
Helixi185 – 187Combined sources3
Beta strandi189 – 193Combined sources5
Beta strandi198 – 203Combined sources6
Beta strandi205 – 214Combined sources10
Beta strandi217 – 223Combined sources7
Turni224 – 226Combined sources3
Beta strandi229 – 234Combined sources6
Beta strandi240 – 245Combined sources6
Beta strandi253 – 258Combined sources6
Beta strandi263 – 267Combined sources5
Turni268 – 270Combined sources3
Beta strandi273 – 277Combined sources5
Beta strandi284 – 289Combined sources6
Beta strandi293 – 300Combined sources8
Beta strandi305 – 309Combined sources5
Turni310 – 313Combined sources4
Beta strandi314 – 319Combined sources6
Beta strandi328 – 333Combined sources6
Beta strandi337 – 344Combined sources8
Beta strandi349 – 353Combined sources5
Turni354 – 356Combined sources3
Beta strandi358 – 363Combined sources6
Beta strandi370 – 375Combined sources6
Beta strandi382 – 386Combined sources5
Beta strandi389 – 394Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95B/D43-395[»]
ProteinModelPortaliP62881
SMRiP62881
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62881

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 142WD 1Add BLAST40
Repeati145 – 184WD 2Add BLAST40
Repeati193 – 234WD 3Add BLAST42
Repeati236 – 278WD 4Add BLAST43
Repeati279 – 318WD 5Add BLAST40
Repeati320 – 362WD 6Add BLAST43
Repeati365 – 394WD 7Add BLAST30

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IU55 Eukaryota
ENOG410YDDC LUCA
GeneTreeiENSGT00760000119239
HOGENOMiHOG000176356
HOVERGENiHBG000188
InParanoidiP62881
KOiK04539
OMAiNVFGSCD
OrthoDBiEOG091G0A7T
PhylomeDBiP62881
TreeFamiTF106149

Family and domain databases

Gene3Di2.130.10.101 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR016346 Guanine_nucleotide-bd_bsu
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19850 PTHR19850, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 5 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62881-1) [UniParc]FASTAAdd to basket
Also known as: Long, Beta-5L, Gbeta5L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE
60 70 80 90 100
NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT
110 120 130 140 150
LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV
160 170 180 190 200
MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA
210 220 230 240 250
CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET
260 270 280 290 300
GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS
310 320 330 340 350
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN
360 370 380 390
VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA
Length:395
Mass (Da):43,565
Last modified:August 16, 2004 - v1
Checksum:i972A41AAEC4CB3F8
GO
Isoform 2 (identifier: P62881-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:353
Mass (Da):38,732
Checksum:i30FCF51C125A024D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0087661 – 42Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34290 mRNA Translation: AAA93084.1
U69145 mRNA Translation: AAC52886.1
BC016135 mRNA Translation: AAH16135.1
CCDSiCCDS40692.1
PIRiA54969
RefSeqiNP_034443.1, NM_010313.2 [P62881-1]
NP_619733.1, NM_138719.5 [P62881-2]
UniGeneiMm.17604

Genome annotation databases

EnsembliENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192 [P62881-1]
ENSMUST00000213990; ENSMUSP00000149938; ENSMUSG00000032192 [P62881-1]
ENSMUST00000215875; ENSMUSP00000150492; ENSMUSG00000032192 [P62881-2]
GeneIDi14697
KEGGimmu:14697
UCSCiuc009qry.2 mouse

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGNB5_MOUSE
AccessioniPrimary (citable) accession number: P62881
Secondary accession number(s): O35354, P54314, Q91WB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome