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Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

Gnb5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-MMU-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-MMU-420092. Glucagon-type ligand receptors.
R-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene namesi
Name:Gnb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:101848. Gnb5.

Subcellular locationi

  • Membrane 2 Publications

GO - Cellular componenti

  • cytosol Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
  • photoreceptor inner segment Source: MGI
  • photoreceptor outer segment Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277061 – 395Guanine nucleotide-binding protein subunit beta-5Add BLAST395

Proteomic databases

MaxQBiP62881.
PaxDbiP62881.
PeptideAtlasiP62881.
PRIDEiP62881.

PTM databases

iPTMnetiP62881.
PhosphoSitePlusiP62881.

Expressioni

Tissue specificityi

Isoform 1 is only detected in retina (PubMed:8910430). Isoform 2 is detected in brain (at protein level) (PubMed:8071339). Isoform 2 is detected in brain (PubMed:8071339).2 Publications

Gene expression databases

BgeeiENSMUSG00000032192.
GenevisibleiP62881. MM.

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 subunits; alpha, beta and gamma. Interacts with RGS9 (PubMed:18204463). Component of a complex composed of RGS9 (isoform RGS9-1), GNB5 and RGS9BP (PubMed:12119397, PubMed:16908407). Interacts with RGS6 and RGS7 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199981. 1 interactor.
DIPiDIP-264N.
IntActiP62881. 3 interactors.
MINTiMINT-4095704.
STRINGi10090.ENSMUSP00000076155.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi52 – 73Combined sources22
Helixi80 – 83Combined sources4
Turni84 – 86Combined sources3
Beta strandi97 – 102Combined sources6
Beta strandi108 – 113Combined sources6
Beta strandi117 – 124Combined sources8
Beta strandi127 – 133Combined sources7
Turni134 – 136Combined sources3
Beta strandi139 – 144Combined sources6
Beta strandi146 – 148Combined sources3
Beta strandi152 – 155Combined sources4
Beta strandi159 – 166Combined sources8
Beta strandi169 – 175Combined sources7
Helixi185 – 187Combined sources3
Beta strandi189 – 193Combined sources5
Beta strandi198 – 203Combined sources6
Beta strandi205 – 214Combined sources10
Beta strandi217 – 223Combined sources7
Turni224 – 226Combined sources3
Beta strandi229 – 234Combined sources6
Beta strandi240 – 245Combined sources6
Beta strandi253 – 258Combined sources6
Beta strandi263 – 267Combined sources5
Turni268 – 270Combined sources3
Beta strandi273 – 277Combined sources5
Beta strandi284 – 289Combined sources6
Beta strandi293 – 300Combined sources8
Beta strandi305 – 309Combined sources5
Turni310 – 313Combined sources4
Beta strandi314 – 319Combined sources6
Beta strandi328 – 333Combined sources6
Beta strandi337 – 344Combined sources8
Beta strandi349 – 353Combined sources5
Turni354 – 356Combined sources3
Beta strandi358 – 363Combined sources6
Beta strandi370 – 375Combined sources6
Beta strandi382 – 386Combined sources5
Beta strandi389 – 394Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95B/D43-395[»]
ProteinModelPortaliP62881.
SMRiP62881.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62881.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 142WD 1Add BLAST40
Repeati145 – 184WD 2Add BLAST40
Repeati193 – 234WD 3Add BLAST42
Repeati236 – 278WD 4Add BLAST43
Repeati279 – 318WD 5Add BLAST40
Repeati320 – 362WD 6Add BLAST43
Repeati365 – 394WD 7Add BLAST30

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IU55. Eukaryota.
ENOG410YDDC. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62881.
KOiK04539.
OMAiYCSTCAE.
OrthoDBiEOG091G0A7T.
PhylomeDBiP62881.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62881-1) [UniParc]FASTAAdd to basket
Also known as: Long, Beta-5L, Gbeta5L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE
60 70 80 90 100
NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT
110 120 130 140 150
LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV
160 170 180 190 200
MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA
210 220 230 240 250
CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET
260 270 280 290 300
GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS
310 320 330 340 350
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN
360 370 380 390
VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA
Length:395
Mass (Da):43,565
Last modified:August 16, 2004 - v1
Checksum:i972A41AAEC4CB3F8
GO
Isoform 2 (identifier: P62881-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:353
Mass (Da):38,732
Checksum:i30FCF51C125A024D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0087661 – 42Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34290 mRNA. Translation: AAA93084.1.
U69145 mRNA. Translation: AAC52886.1.
BC016135 mRNA. Translation: AAH16135.1.
CCDSiCCDS40692.1.
PIRiA54969.
RefSeqiNP_034443.1. NM_010313.2. [P62881-1]
NP_619733.1. NM_138719.5. [P62881-2]
UniGeneiMm.17604.

Genome annotation databases

EnsembliENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192. [P62881-1]
GeneIDi14697.
KEGGimmu:14697.
UCSCiuc009qry.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34290 mRNA. Translation: AAA93084.1.
U69145 mRNA. Translation: AAC52886.1.
BC016135 mRNA. Translation: AAH16135.1.
CCDSiCCDS40692.1.
PIRiA54969.
RefSeqiNP_034443.1. NM_010313.2. [P62881-1]
NP_619733.1. NM_138719.5. [P62881-2]
UniGeneiMm.17604.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95B/D43-395[»]
ProteinModelPortaliP62881.
SMRiP62881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199981. 1 interactor.
DIPiDIP-264N.
IntActiP62881. 3 interactors.
MINTiMINT-4095704.
STRINGi10090.ENSMUSP00000076155.

PTM databases

iPTMnetiP62881.
PhosphoSitePlusiP62881.

Proteomic databases

MaxQBiP62881.
PaxDbiP62881.
PeptideAtlasiP62881.
PRIDEiP62881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192. [P62881-1]
GeneIDi14697.
KEGGimmu:14697.
UCSCiuc009qry.2. mouse.

Organism-specific databases

CTDi10681.
MGIiMGI:101848. Gnb5.

Phylogenomic databases

eggNOGiENOG410IU55. Eukaryota.
ENOG410YDDC. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62881.
KOiK04539.
OMAiYCSTCAE.
OrthoDBiEOG091G0A7T.
PhylomeDBiP62881.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-MMU-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-MMU-420092. Glucagon-type ligand receptors.
R-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

ChiTaRSiGnb5. mouse.
EvolutionaryTraceiP62881.
PROiP62881.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032192.
GenevisibleiP62881. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB5_MOUSE
AccessioniPrimary (citable) accession number: P62881
Secondary accession number(s): O35354, P54314, Q91WB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.