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P62881 (GBB5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene names
Name:Gnb5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure

G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1) Gbeta5 (GNB5) and RGS9BP.

Tissue specificity

Brain and at much reduced levels in kidney. Isoform 1 is retinal specific. Ref.2

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P62881-1)

Also known as: Long; Beta-5L; Gbeta5L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P62881-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Guanine nucleotide-binding protein subunit beta-5
PRO_0000127706

Regions

Repeat103 – 14240WD 1
Repeat145 – 18440WD 2
Repeat193 – 23442WD 3
Repeat236 – 27843WD 4
Repeat279 – 31840WD 5
Repeat320 – 36243WD 6
Repeat365 – 39430WD 7

Natural variations

Alternative sequence1 – 4242Missing in isoform 2.
VSP_008766

Secondary structure

...................................................................... 395
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (Beta-5L) (Gbeta5L) [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 972A41AAEC4CB3F8

FASTA39543,565
        10         20         30         40         50         60 
MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE NETLASLKSE 

        70         80         90        100        110        120 
AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT LKGHGNKVLC MDWCKDKRRI 

       130        140        150        160        170        180 
VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV MACAYAPSGC AIACGGLDNK CSVYPLTFDK 

       190        200        210        220        230        240 
NENMAAKKKS VAMHTNYLSA CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV 

       250        260        270        280        290        300 
LCLDLAPSET GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS 

       310        320        330        340        350        360 
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN VWDVLKGSRV 

       370        380        390 
SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA 

« Hide

Isoform 2 [UniParc] [UniParc].

Checksum: 30FCF51C125A024D
Show »

FASTA35338,732

References

« Hide 'large scale' references
[1]"A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C."
Watson A.J., Katz A., Simon M.I.
J. Biol. Chem. 269:22150-22156(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina."
Watson A.J., Aragay A.M., Slepak V.Z., Simon M.I.
J. Biol. Chem. 271:28154-28160(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye and Retina.
[4]Lubec G., Klug S.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 75-86 AND 322-338, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Hippocampus.
[5]"R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1."
Hu G., Wensel T.G.
Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS9 AND RGS9BP.
Strain: C57BL/6 X 129.
Tissue: Retina.
[6]"RGS expression rate-limits recovery of rod photoresponses."
Krispel C.M., Chen D., Melling N., Chen Y.-J., Martemyanov K.A., Quillinan N., Arshavsky V.Y., Wensel T.G., Chen C.-K., Burns M.E.
Neuron 51:409-416(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS9 AND RGS9BP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L34290 mRNA. Translation: AAA93084.1.
U69145 mRNA. Translation: AAC52886.1.
BC016135 mRNA. Translation: AAH16135.1.
PIRA54969.
RefSeqNP_034443.1. NM_010313.2.
NP_619733.1. NM_138719.5.
UniGeneMm.17604.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2PBIX-ray1.95B/D43-395[»]
ProteinModelPortalP62881.
SMRP62881. Positions 43-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199981. 1 interaction.
DIPDIP-264N.
IntActP62881. 3 interactions.
MINTMINT-4095704.

PTM databases

PhosphoSiteP62881.

Proteomic databases

PaxDbP62881.
PRIDEP62881.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076889; ENSMUSP00000076155; ENSMUSG00000032192. [P62881-1]
GeneID14697.
KEGGmmu:14697.
UCSCuc009qry.1. mouse.

Organism-specific databases

CTD10681.
MGIMGI:101848. Gnb5.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00740000115438.
HOGENOMHOG000176356.
HOVERGENHBG000188.
InParanoidP62881.
KOK04539.
OMADQTFLVN.
OrthoDBEOG7GN2N5.
PhylomeDBP62881.
TreeFamTF106149.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressP62881.
BgeeP62881.
GenevestigatorP62881.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 7 hits.
[Graphical view]
PIRSFPIRSF002394. GN-bd_beta. 1 hit.
PRINTSPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP62881.
NextBio286651.
PROP62881.
SOURCESearch...

Entry information

Entry nameGBB5_MOUSE
AccessionPrimary (citable) accession number: P62881
Secondary accession number(s): O35354, P54314, Q91WB3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot