Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P62880

- GBB2_MOUSE

UniProt

P62880 - GBB2_MOUSE

Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2

Gene

Gnb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. calcium channel regulator activity Source: Ensembl
    2. GTPase activity Source: MGI
    3. protein binding Source: MGI
    4. protein complex binding Source: MGI
    5. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. G-protein coupled receptor signaling pathway Source: MGI
    2. GTP catabolic process Source: GOC

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_208837. Activation of G protein gated Potassium channels.
    REACT_210399. Glucagon signaling in metabolic regulation.
    REACT_213947. Regulation of water balance by renal Aquaporins.
    REACT_221970. Ca2+ pathway.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
    Alternative name(s):
    G protein subunit beta-2
    Transducin beta chain 2
    Gene namesi
    Name:Gnb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:95784. Gnb2.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity

    GO - Cellular componenti

    1. cell body Source: Ensembl
    2. heterotrimeric G-protein complex Source: MGI
    3. lysosomal membrane Source: Ensembl
    4. perinuclear region of cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2PRO_0000127696Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei239 – 2391Phosphotyrosine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP62880.
    PaxDbiP62880.
    PRIDEiP62880.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00162780.

    PTM databases

    PhosphoSiteiP62880.

    Expressioni

    Developmental stagei

    Expressed in meiotically incompetent oocytes. Expression increases in fully grown meiotically competent oocytes. Expression then decreases during metaphase-II arrested eggs, one-cell embryo, two-cell embryo and eight-cell embryo stages, and increases again during blastocyst stage.1 Publication

    Gene expression databases

    BgeeiP62880.
    GenevestigatoriP62880.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2 By similarity. Interacts with ATXN10 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi199977. 4 interactions.
    IntActiP62880. 5 interactions.
    MINTiMINT-1865380.
    STRINGi10090.ENSMUSP00000106656.

    Structurei

    3D structure databases

    ProteinModelPortaliP62880.
    SMRiP62880. Positions 1-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati53 – 8331WD 1Add
    BLAST
    Repeati95 – 12531WD 2Add
    BLAST
    Repeati141 – 17030WD 3Add
    BLAST
    Repeati182 – 21231WD 4Add
    BLAST
    Repeati224 – 25431WD 5Add
    BLAST
    Repeati268 – 29831WD 6Add
    BLAST
    Repeati310 – 34031WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000176356.
    HOVERGENiHBG000188.
    InParanoidiP62880.
    KOiK04537.
    OrthoDBiEOG7GN2N5.
    PhylomeDBiP62880.
    TreeFamiTF106149.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62880-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSELEQLRQE AEQLRNQIRD ARKACGDSTL TQITAGLDPV GRIQMRTRRT    50
    LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV 100
    MTCAYAPSGN FVACGGLDNI CSIYSLKTRE GNVRVSRELP GHTGYLSCCR 150
    FLDDNQIITS SGDTTCALWD IETGQQTVGF AGHSGDVMSL SLAPDGRTFV 200
    SGACDASIKL WDVRDSMCRQ TFIGHESDIN AVAFFPNGYA FTTGSDDATC 250
    RLFDLRADQE LLMYSHDNII CGITSVAFSR SGRLLLAGYD DFNCNIWDAM 300
    KGDRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN 340
    Length:340
    Mass (Da):37,331
    Last modified:January 23, 2007 - v3
    Checksum:i5D08FFA240ADEEE6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061A → G in AAC72250. 1 PublicationCurated
    Sequence conflicti248 – 2481A → S in AAC72250. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34960 mRNA. Translation: AAC72250.1.
    AB045007 Genomic DNA. Translation: BAB19816.1.
    AF312033 Genomic DNA. Translation: AAK28828.1.
    BC029077 mRNA. Translation: AAH29077.1.
    BC059942 mRNA. Translation: AAH59942.1.
    BC062178 mRNA. Translation: AAH62178.1.
    U38505 mRNA. Translation: AAB01736.1.
    CCDSiCCDS19769.1.
    RefSeqiNP_034442.1. NM_010312.4.
    XP_006504616.1. XM_006504553.1.
    UniGeneiMm.30141.

    Genome annotation databases

    EnsembliENSMUST00000031726; ENSMUSP00000031726; ENSMUSG00000029713.
    ENSMUST00000150063; ENSMUSP00000129353; ENSMUSG00000029713.
    GeneIDi14693.
    KEGGimmu:14693.
    UCSCiuc009act.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34960 mRNA. Translation: AAC72250.1 .
    AB045007 Genomic DNA. Translation: BAB19816.1 .
    AF312033 Genomic DNA. Translation: AAK28828.1 .
    BC029077 mRNA. Translation: AAH29077.1 .
    BC059942 mRNA. Translation: AAH59942.1 .
    BC062178 mRNA. Translation: AAH62178.1 .
    U38505 mRNA. Translation: AAB01736.1 .
    CCDSi CCDS19769.1.
    RefSeqi NP_034442.1. NM_010312.4.
    XP_006504616.1. XM_006504553.1.
    UniGenei Mm.30141.

    3D structure databases

    ProteinModelPortali P62880.
    SMRi P62880. Positions 1-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199977. 4 interactions.
    IntActi P62880. 5 interactions.
    MINTi MINT-1865380.
    STRINGi 10090.ENSMUSP00000106656.

    PTM databases

    PhosphoSitei P62880.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00162780.

    Proteomic databases

    MaxQBi P62880.
    PaxDbi P62880.
    PRIDEi P62880.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031726 ; ENSMUSP00000031726 ; ENSMUSG00000029713 .
    ENSMUST00000150063 ; ENSMUSP00000129353 ; ENSMUSG00000029713 .
    GeneIDi 14693.
    KEGGi mmu:14693.
    UCSCi uc009act.1. mouse.

    Organism-specific databases

    CTDi 2783.
    MGIi MGI:95784. Gnb2.

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000176356.
    HOVERGENi HBG000188.
    InParanoidi P62880.
    KOi K04537.
    OrthoDBi EOG7GN2N5.
    PhylomeDBi P62880.
    TreeFami TF106149.

    Enzyme and pathway databases

    Reactomei REACT_208837. Activation of G protein gated Potassium channels.
    REACT_210399. Glucagon signaling in metabolic regulation.
    REACT_213947. Regulation of water balance by renal Aquaporins.
    REACT_221970. Ca2+ pathway.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Miscellaneous databases

    ChiTaRSi GNB2. mouse.
    NextBioi 10963.
    PROi P62880.
    SOURCEi Search...

    Gene expression databases

    Bgeei P62880.
    Genevestigatori P62880.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Kuroda S., Tokunaga C., Konishi H., Kikkawa U.
      Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/Kaplan.
    2. "Genomic organization of the murine G protein beta subunit genes and related processed pseudogenes."
      Kitanaka J., Wang X., Kitanaka N., Hembree C.M., Uhl G.R.
      DNA Seq. 12:345-354(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    3. "Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5."
      Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P., Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W., Koop B.F.
      Nucleic Acids Res. 29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Embryo.
    5. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 23-42; 58-96; 138-150; 198-209; 257-280 AND 284-301, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    6. "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
      Williams C.J., Schultz R.M., Kopf G.S.
      Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 182-297, DEVELOPMENTAL STAGE.
      Strain: CF-1.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiGBB2_MOUSE
    AccessioniPrimary (citable) accession number: P62880
    Secondary accession number(s): P11016, P54312
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3