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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2

Gene

Gnb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: MGI
  • metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiREACT_272374. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_297430. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_308732. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_339334. Activation of G protein gated Potassium channels.
REACT_339920. Glucagon-type ligand receptors.
REACT_345203. Glucagon signaling in metabolic regulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
Alternative name(s):
G protein subunit beta-2
Transducin beta chain 2
Gene namesi
Name:Gnb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95784. Gnb2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2PRO_0000127696Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei239 – 2391Phosphotyrosine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP62880.
PaxDbiP62880.
PRIDEiP62880.

2D gel databases

REPRODUCTION-2DPAGEIPI00162780.

PTM databases

PhosphoSiteiP62880.

Expressioni

Developmental stagei

Expressed in meiotically incompetent oocytes. Expression increases in fully grown meiotically competent oocytes. Expression then decreases during metaphase-II arrested eggs, one-cell embryo, two-cell embryo and eight-cell embryo stages, and increases again during blastocyst stage.1 Publication

Gene expression databases

BgeeiP62880.
ExpressionAtlasiP62880. baseline and differential.
GenevisibleiP62880. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2 (By similarity). Interacts with ATXN10 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199977. 4 interactions.
IntActiP62880. 5 interactions.
MINTiMINT-1865380.
STRINGi10090.ENSMUSP00000031726.

Structurei

3D structure databases

ProteinModelPortaliP62880.
SMRiP62880. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 8331WD 1Add
BLAST
Repeati95 – 12531WD 2Add
BLAST
Repeati141 – 17030WD 3Add
BLAST
Repeati182 – 21231WD 4Add
BLAST
Repeati224 – 25431WD 5Add
BLAST
Repeati268 – 29831WD 6Add
BLAST
Repeati310 – 34031WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62880.
KOiK04537.
OrthoDBiEOG7GN2N5.
PhylomeDBiP62880.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELEQLRQE AEQLRNQIRD ARKACGDSTL TQITAGLDPV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN FVACGGLDNI CSIYSLKTRE GNVRVSRELP GHTGYLSCCR
160 170 180 190 200
FLDDNQIITS SGDTTCALWD IETGQQTVGF AGHSGDVMSL SLAPDGRTFV
210 220 230 240 250
SGACDASIKL WDVRDSMCRQ TFIGHESDIN AVAFFPNGYA FTTGSDDATC
260 270 280 290 300
RLFDLRADQE LLMYSHDNII CGITSVAFSR SGRLLLAGYD DFNCNIWDAM
310 320 330 340
KGDRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,331
Last modified:January 23, 2007 - v3
Checksum:i5D08FFA240ADEEE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061A → G in AAC72250 (Ref. 1) Curated
Sequence conflicti248 – 2481A → S in AAC72250 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34960 mRNA. Translation: AAC72250.1.
AB045007 Genomic DNA. Translation: BAB19816.1.
AF312033 Genomic DNA. Translation: AAK28828.1.
BC029077 mRNA. Translation: AAH29077.1.
BC059942 mRNA. Translation: AAH59942.1.
BC062178 mRNA. Translation: AAH62178.1.
U38505 mRNA. Translation: AAB01736.1.
CCDSiCCDS19769.1.
RefSeqiNP_034442.1. NM_010312.4.
XP_006504616.1. XM_006504553.2.
UniGeneiMm.30141.

Genome annotation databases

EnsembliENSMUST00000031726; ENSMUSP00000031726; ENSMUSG00000029713.
ENSMUST00000150063; ENSMUSP00000129353; ENSMUSG00000029713.
GeneIDi14693.
KEGGimmu:14693.
UCSCiuc009act.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34960 mRNA. Translation: AAC72250.1.
AB045007 Genomic DNA. Translation: BAB19816.1.
AF312033 Genomic DNA. Translation: AAK28828.1.
BC029077 mRNA. Translation: AAH29077.1.
BC059942 mRNA. Translation: AAH59942.1.
BC062178 mRNA. Translation: AAH62178.1.
U38505 mRNA. Translation: AAB01736.1.
CCDSiCCDS19769.1.
RefSeqiNP_034442.1. NM_010312.4.
XP_006504616.1. XM_006504553.2.
UniGeneiMm.30141.

3D structure databases

ProteinModelPortaliP62880.
SMRiP62880. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199977. 4 interactions.
IntActiP62880. 5 interactions.
MINTiMINT-1865380.
STRINGi10090.ENSMUSP00000031726.

PTM databases

PhosphoSiteiP62880.

2D gel databases

REPRODUCTION-2DPAGEIPI00162780.

Proteomic databases

MaxQBiP62880.
PaxDbiP62880.
PRIDEiP62880.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031726; ENSMUSP00000031726; ENSMUSG00000029713.
ENSMUST00000150063; ENSMUSP00000129353; ENSMUSG00000029713.
GeneIDi14693.
KEGGimmu:14693.
UCSCiuc009act.1. mouse.

Organism-specific databases

CTDi2783.
MGIiMGI:95784. Gnb2.

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62880.
KOiK04537.
OrthoDBiEOG7GN2N5.
PhylomeDBiP62880.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiREACT_272374. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_297430. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_308732. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_339334. Activation of G protein gated Potassium channels.
REACT_339920. Glucagon-type ligand receptors.
REACT_345203. Glucagon signaling in metabolic regulation.

Miscellaneous databases

ChiTaRSiGnb2. mouse.
NextBioi286635.
PROiP62880.
SOURCEiSearch...

Gene expression databases

BgeeiP62880.
ExpressionAtlasiP62880. baseline and differential.
GenevisibleiP62880. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Kuroda S., Tokunaga C., Konishi H., Kikkawa U.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/Kaplan.
  2. "Genomic organization of the murine G protein beta subunit genes and related processed pseudogenes."
    Kitanaka J., Wang X., Kitanaka N., Hembree C.M., Uhl G.R.
    DNA Seq. 12:345-354(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5."
    Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P., Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W., Koop B.F.
    Nucleic Acids Res. 29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Embryo.
  5. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 23-42; 58-96; 138-150; 198-209; 257-280 AND 284-301, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.
  6. "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
    Williams C.J., Schultz R.M., Kopf G.S.
    Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 182-297, DEVELOPMENTAL STAGE.
    Strain: CF-1.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiGBB2_MOUSE
AccessioniPrimary (citable) accession number: P62880
Secondary accession number(s): P11016, P54312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.