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P62879 (GBB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
Alternative name(s):
G protein subunit beta-2
Transducin beta chain 2
Gene names
Name:GNB2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. Interacts with ATXN10. Ref.7 Ref.8 Ref.10

Subcellular location

Cytoplasmperinuclear region Ref.8.

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRepeat
WD repeat
   Molecular functionTransducer
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 7649993. Source: ProtInc

GTP catabolic process

Traceable author statement PubMed 2902634. Source: GOC

cellular response to glucagon stimulus

Traceable author statement. Source: Reactome

energy reserve metabolic process

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

synaptic transmission

Traceable author statement. Source: Reactome

   Cellular_componentcell body

Inferred from electronic annotation. Source: Ensembl

extracellular vesicular exosome

Inferred from direct assay PubMed 19199708PubMed 20458337PubMed 23376485. Source: UniProt

lysosomal membrane

Inferred from direct assay PubMed 17897319. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from direct assay Ref.8. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionGTPase activity

Traceable author statement PubMed 2902634. Source: ProtInc

GTPase binding

Inferred from physical interaction Ref.10. Source: UniProtKB

calcium channel regulator activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

protein complex binding

Inferred from direct assay PubMed 23209302. Source: MGI

signal transducer activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
PRO_0000127695

Regions

Repeat53 – 8331WD 1
Repeat95 – 12531WD 2
Repeat141 – 17030WD 3
Repeat182 – 21231WD 4
Repeat224 – 25431WD 5
Repeat268 – 29831WD 6
Repeat310 – 34031WD 7

Amino acid modifications

Modified residue21N-acetylserine Ref.6 Ref.9
Modified residue2391Phosphotyrosine By similarity

Experimental info

Sequence conflict1951D → N in AAA35922. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P62879 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5D08FFA240ADEEE6

FASTA34037,331
        10         20         30         40         50         60 
MSELEQLRQE AEQLRNQIRD ARKACGDSTL TQITAGLDPV GRIQMRTRRT LRGHLAKIYA 

        70         80         90        100        110        120 
MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN FVACGGLDNI 

       130        140        150        160        170        180 
CSIYSLKTRE GNVRVSRELP GHTGYLSCCR FLDDNQIITS SGDTTCALWD IETGQQTVGF 

       190        200        210        220        230        240 
AGHSGDVMSL SLAPDGRTFV SGACDASIKL WDVRDSMCRQ TFIGHESDIN AVAFFPNGYA 

       250        260        270        280        290        300 
FTTGSDDATC RLFDLRADQE LLMYSHDNII CGITSVAFSR SGRLLLAGYD DFNCNIWDAM 

       310        320        330        340 
KGDRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN 

« Hide

References

« Hide 'large scale' references
[1]"Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning."
Fong H.K.W., Amatruda T.T. III, Birren B.W., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 84:3792-3796(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A second form of the beta subunit of signal-transducing G proteins."
Gao B., Gilman A.G., Robishaw J.D.
Proc. Natl. Acad. Sci. U.S.A. 84:6122-6125(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes."
Gloeckner G., Scherer S., Schattevoy R., Boright A.P., Weber J., Tsui L.-C., Rosenthal A.
Genome Res. 8:1060-1073(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas, Skin and Uterus.
[6]Bienvenut W.V.
Submitted (JUL-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-15 AND 58-78, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Melanoma.
[7]"G Protein betagamma subunits stimulate p114RhoGEF, a guanine nucleotide exchange factor for RhoA and Rac1: regulation of cell shape and reactive oxygen species production."
Niu J., Profirovic J., Pan H., Vaiskunaite R., Voyno-Yasenetskaya T.
Circ. Res. 93:848-856(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARHGEF18.
[8]"Ataxin 10 induces neuritogenesis via interaction with G-protein beta2 subunit."
Waragai M., Nagamitsu S., Xu W., Li Y.J., Lin X., Ashizawa T.
J. Neurosci. Res. 83:1170-1178(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATXN10.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The cationic region of Rhes mediates its interactions with specific Gbeta subunits."
Hill C., Goddard A., Ladds G., Davey J.
Cell. Physiol. Biochem. 23:1-8(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RASD2.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16514 mRNA. Translation: AAA03179.1.
M36429 mRNA. Translation: AAA63264.1.
M16538 mRNA. Translation: AAA35922.1.
AF053356 Genomic DNA. Translation: AAC78794.1.
AF501883 mRNA. Translation: AAM15919.1.
BC010073 mRNA. Translation: AAH10073.1.
BC012348 mRNA. Translation: AAH12348.1.
BC068003 mRNA. Translation: AAH68003.1.
CCDSCCDS5703.1.
PIRRGHUB2. B26617.
RefSeqNP_005264.2. NM_005273.3.
UniGeneHs.185172.

3D structure databases

ProteinModelPortalP62879.
SMRP62879. Positions 1-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109045. 52 interactions.
IntActP62879. 27 interactions.
MINTMINT-1139073.
STRING9606.ENSP00000305260.

PTM databases

PhosphoSiteP62879.

Polymorphism databases

DMDM51317304.

2D gel databases

OGPP62879.
REPRODUCTION-2DPAGEP62879.

Proteomic databases

MaxQBP62879.
PaxDbP62879.
PRIDEP62879.

Protocols and materials databases

DNASU2783.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000303210; ENSP00000305260; ENSG00000172354.
ENST00000393924; ENSP00000377501; ENSG00000172354.
ENST00000393926; ENSP00000377503; ENSG00000172354.
GeneID2783.
KEGGhsa:2783.
UCSCuc003uwb.3. human.

Organism-specific databases

CTD2783.
GeneCardsGC07P100271.
HGNCHGNC:4398. GNB2.
HPACAB010032.
HPA040736.
MIM139390. gene.
neXtProtNX_P62879.
PharmGKBPA28778.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000176356.
HOVERGENHBG000188.
InParanoidP62879.
KOK04537.
OMAWDIETSQ.
OrthoDBEOG7GN2N5.
PhylomeDBP62879.
TreeFamTF106149.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_604. Hemostasis.
SignaLinkP62879.

Gene expression databases

ArrayExpressP62879.
BgeeP62879.
CleanExHS_GNB2.
GenevestigatorP62879.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 7 hits.
[Graphical view]
PIRSFPIRSF002394. GN-bd_beta. 1 hit.
PRINTSPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGNB2.
GenomeRNAi2783.
NextBio10963.
PROP62879.
SOURCESearch...

Entry information

Entry nameGBB2_HUMAN
AccessionPrimary (citable) accession number: P62879
Secondary accession number(s): P11016, P54312
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM