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P62875

- RPAB5_HUMAN

UniProt

P62875 - RPAB5_HUMAN

Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC5

Gene

POLR2L

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2L/RBP10 is part of the core element with the central large cleft By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71ZincBy similarity
    Metal bindingi10 – 101ZincBy similarity
    Metal bindingi44 – 441ZincBy similarity
    Metal bindingi45 – 451ZincBy similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: ProtInc
    3. zinc ion binding Source: RefGenome

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: Reactome
    2. DNA repair Source: Reactome
    3. gene expression Source: Reactome
    4. innate immune response Source: Reactome
    5. mRNA splicing, via spliceosome Source: Reactome
    6. nucleotide-excision repair Source: Reactome
    7. positive regulation of type I interferon production Source: Reactome
    8. positive regulation of viral transcription Source: Reactome
    9. regulation of transcription from RNA polymerase I promoter Source: ProtInc
    10. RNA splicing Source: Reactome
    11. termination of RNA polymerase III transcription Source: Reactome
    12. transcription-coupled nucleotide-excision repair Source: Reactome
    13. transcription elongation from RNA polymerase III promoter Source: Reactome
    14. transcription elongation from RNA polymerase II promoter Source: Reactome
    15. transcription from RNA polymerase III promoter Source: Reactome
    16. transcription from RNA polymerase II promoter Source: UniProtKB
    17. transcription from RNA polymerase I promoter Source: GOC
    18. transcription initiation from RNA polymerase II promoter Source: Reactome
    19. viral process Source: Reactome

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
    REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_63. RNA Polymerase III Transcription Termination.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_756. RNA Polymerase III Chain Elongation.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerases I, II, and III subunit RPABC5
    Short name:
    RNA polymerases I, II, and III subunit ABC5
    Alternative name(s):
    DNA-directed RNA polymerase III subunit L
    RNA polymerase II 7.6 kDa subunit
    Short name:
    RPB7.6
    RPB10 homolog
    Gene namesi
    Name:POLR2L
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:9199. POLR2L.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. DNA-directed RNA polymerase I complex Source: RefGenome
    3. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    4. DNA-directed RNA polymerase III complex Source: RefGenome
    5. nucleoplasm Source: Reactome
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33519.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6767DNA-directed RNA polymerases I, II, and III subunit RPABC5PRO_0000121333Add
    BLAST

    Proteomic databases

    MaxQBiP62875.
    PaxDbiP62875.
    PRIDEiP62875.

    PTM databases

    PhosphoSiteiP62875.

    Expressioni

    Gene expression databases

    BgeeiP62875.
    CleanExiHS_POLR2L.
    GenevestigatoriP62875.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

    Protein-protein interaction databases

    BioGridi111437. 23 interactions.
    IntActiP62875. 11 interactions.
    MINTiMINT-1132504.
    STRINGi9606.ENSP00000324124.

    Structurei

    3D structure databases

    ProteinModelPortaliP62875.
    SMRiP62875. Positions 1-67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1644.
    HOVERGENiHBG061475.
    InParanoidiP62875.
    KOiK03007.
    OMAiRRAKFIF.
    OrthoDBiEOG79CZ2Z.
    PhylomeDBiP62875.
    TreeFamiTF103046.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    HAMAPiMF_00250. RNApol_arch_N.
    InterProiIPR009057. Homeodomain-like.
    IPR023580. RNA_pol_su_RPB10.
    IPR020789. RNA_pol_suN_Zn-BS.
    IPR000268. RNAP_N/Rpb10.
    [Graphical view]
    PfamiPF01194. RNA_pol_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005653. RNA_pol_N/8_sub. 1 hit.
    ProDomiPD006539. RNA_pol_N/8_sub. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF46924. SSF46924. 1 hit.
    PROSITEiPS01112. RNA_POL_N_8KD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62875-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL   50
    AHVDLIEKLL NYAPLEK 67
    Length:67
    Mass (Da):7,645
    Last modified:August 16, 2004 - v1
    Checksum:i9E8A72F667FE7EC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37690 mRNA. Translation: AAA91459.1.
    CR536565 mRNA. Translation: CAG38802.1.
    AK311997 mRNA. Translation: BAG34935.1.
    BC005903 mRNA. Translation: AAH05903.1.
    BC018649 mRNA. Translation: AAH18649.1.
    Z47729 Genomic DNA. Translation: CAA87658.1.
    Z47728 Genomic DNA. Translation: CAA87657.1.
    CCDSiCCDS7720.1.
    PIRiS53015.
    RefSeqiNP_066951.1. NM_021128.4.
    UniGeneiHs.441072.

    Genome annotation databases

    EnsembliENST00000322028; ENSP00000324124; ENSG00000177700.
    ENST00000534030; ENSP00000432807; ENSG00000177700.
    GeneIDi5441.
    KEGGihsa:5441.
    UCSCiuc001lsc.3. human.

    Polymorphism databases

    DMDMi51338657.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37690 mRNA. Translation: AAA91459.1 .
    CR536565 mRNA. Translation: CAG38802.1 .
    AK311997 mRNA. Translation: BAG34935.1 .
    BC005903 mRNA. Translation: AAH05903.1 .
    BC018649 mRNA. Translation: AAH18649.1 .
    Z47729 Genomic DNA. Translation: CAA87658.1 .
    Z47728 Genomic DNA. Translation: CAA87657.1 .
    CCDSi CCDS7720.1.
    PIRi S53015.
    RefSeqi NP_066951.1. NM_021128.4.
    UniGenei Hs.441072.

    3D structure databases

    ProteinModelPortali P62875.
    SMRi P62875. Positions 1-67.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111437. 23 interactions.
    IntActi P62875. 11 interactions.
    MINTi MINT-1132504.
    STRINGi 9606.ENSP00000324124.

    PTM databases

    PhosphoSitei P62875.

    Polymorphism databases

    DMDMi 51338657.

    Proteomic databases

    MaxQBi P62875.
    PaxDbi P62875.
    PRIDEi P62875.

    Protocols and materials databases

    DNASUi 5441.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000322028 ; ENSP00000324124 ; ENSG00000177700 .
    ENST00000534030 ; ENSP00000432807 ; ENSG00000177700 .
    GeneIDi 5441.
    KEGGi hsa:5441.
    UCSCi uc001lsc.3. human.

    Organism-specific databases

    CTDi 5441.
    GeneCardsi GC11M000829.
    HGNCi HGNC:9199. POLR2L.
    MIMi 601189. gene.
    neXtProti NX_P62875.
    PharmGKBi PA33519.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1644.
    HOVERGENi HBG061475.
    InParanoidi P62875.
    KOi K03007.
    OMAi RRAKFIF.
    OrthoDBi EOG79CZ2Z.
    PhylomeDBi P62875.
    TreeFami TF103046.

    Enzyme and pathway databases

    Reactomei REACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
    REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_63. RNA Polymerase III Transcription Termination.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_756. RNA Polymerase III Chain Elongation.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Miscellaneous databases

    GeneWikii POLR2L.
    GenomeRNAii 5441.
    NextBioi 21055.
    PROi P62875.
    SOURCEi Search...

    Gene expression databases

    Bgeei P62875.
    CleanExi HS_POLR2L.
    Genevestigatori P62875.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    HAMAPi MF_00250. RNApol_arch_N.
    InterProi IPR009057. Homeodomain-like.
    IPR023580. RNA_pol_su_RPB10.
    IPR020789. RNA_pol_suN_Zn-BS.
    IPR000268. RNAP_N/Rpb10.
    [Graphical view ]
    Pfami PF01194. RNA_pol_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005653. RNA_pol_N/8_sub. 1 hit.
    ProDomi PD006539. RNA_pol_N/8_sub. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF46924. SSF46924. 1 hit.
    PROSITEi PS01112. RNA_POL_N_8KD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Six human RNA polymerase subunits functionally substitute for their yeast counterparts."
      McKune K., Moore P.A., Hull M.W., Woychik N.A.
      Mol. Cell. Biol. 15:6895-6900(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow and Kidney.
    5. "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
      Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
      Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
    6. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
      Kershnar E., Wu S.-Y., Chiang C.-M.
      J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
    7. "RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor."
      Panov K.I., Panova T.B., Gadal O., Nishiyama K., Saito T., Russell J., Zomerdijk J.C.B.M.
      Mol. Cell. Biol. 26:5436-5448(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX, SUBCELLULAR LOCATION.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRPAB5_HUMAN
    AccessioniPrimary (citable) accession number: P62875
    Secondary accession number(s): P52436, Q6FHX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3