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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC5

Gene

POLR2L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2L/RBP10 is part of the core element with the central large cleft (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71ZincBy similarity
Metal bindingi10 – 101ZincBy similarity
Metal bindingi44 – 441ZincBy similarity
Metal bindingi45 – 451ZincBy similarity

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed RNA polymerase activity Source: ProtInc
  • zinc ion binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_1074. RNA Polymerase I Transcription Termination.
REACT_118823. Cytosolic sensors of pathogen-associated DNA.
REACT_12417. MicroRNA (miRNA) biogenesis.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1470. mRNA Capping.
REACT_1628. Transcription-coupled NER (TC-NER).
REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1753. mRNA Splicing - Minor Pathway.
REACT_1845. Formation of RNA Pol II elongation complex.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_1913. RNA Polymerase I Promoter Escape.
REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_2204. RNA Polymerase I Chain Elongation.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
REACT_2222. Dual incision reaction in TC-NER.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_263856. Transcriptional regulation of pluripotent stem cells.
REACT_263965. NoRC negatively regulates rRNA expression.
REACT_264617. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.
REACT_267790. PIWI-interacting RNA (piRNA) biogenesis.
REACT_268530. Transcriptional regulation by small RNAs.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_467. mRNA Splicing - Major Pathway.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
REACT_6233. Transcription of the HIV genome.
REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_6244. Pausing and recovery of HIV elongation.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6259. HIV elongation arrest and recovery.
REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
REACT_63. RNA Polymerase III Transcription Termination.
REACT_6319. Formation of the HIV-1 Early Elongation Complex.
REACT_6332. HIV Transcription Initiation.
REACT_6344. Tat-mediated HIV elongation arrest and recovery.
REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_756. RNA Polymerase III Chain Elongation.
REACT_833. RNA Polymerase II Transcription Elongation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_846. Formation of the Early Elongation Complex.
REACT_953. RNA Polymerase I Transcription Initiation.
REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Short name:
RNA polymerases I, II, and III subunit ABC5
Alternative name(s):
DNA-directed RNA polymerase III subunit L
RNA polymerase II 7.6 kDa subunit
Short name:
RPB7.6
RPB10 homolog
Gene namesi
Name:POLR2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9199. POLR2L.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • DNA-directed RNA polymerase I complex Source: GO_Central
  • DNA-directed RNA polymerase II, core complex Source: UniProtKB
  • DNA-directed RNA polymerase III complex Source: GO_Central
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33519.

Polymorphism and mutation databases

BioMutaiPOLR2L.
DMDMi51338657.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6767DNA-directed RNA polymerases I, II, and III subunit RPABC5PRO_0000121333Add
BLAST

Proteomic databases

MaxQBiP62875.
PaxDbiP62875.
PRIDEiP62875.

PTM databases

PhosphoSiteiP62875.

Expressioni

Gene expression databases

BgeeiP62875.
CleanExiHS_POLR2L.
GenevisibleiP62875. HS.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BANPQ8N9N53EBI-359527,EBI-744695
COILP384323EBI-359527,EBI-945751
L3MBTL3Q96JM73EBI-359527,EBI-2686809
PHC2Q8IXK03EBI-359527,EBI-713786
RELQ048643EBI-359527,EBI-307352
RPAP1Q9BWH63EBI-359527,EBI-1048085
SPAG5Q96R063EBI-359527,EBI-413317
THAP1Q9NVV93EBI-359527,EBI-741515
TP53BP2Q13625-33EBI-359527,EBI-10175039
ZBTB14O438293EBI-359527,EBI-10176632

Protein-protein interaction databases

BioGridi111437. 47 interactions.
IntActiP62875. 21 interactions.
MINTiMINT-1132504.
STRINGi9606.ENSP00000324124.

Structurei

3D structure databases

ProteinModelPortaliP62875.
SMRiP62875. Positions 1-67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1644.
GeneTreeiENSGT00390000007087.
HOVERGENiHBG061475.
InParanoidiP62875.
KOiK03007.
OMAiKKYCCRR.
OrthoDBiEOG79CZ2Z.
PhylomeDBiP62875.
TreeFamiTF103046.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
HAMAPiMF_00250. RNApol_arch_N.
InterProiIPR009057. Homeodomain-like.
IPR023580. RNA_pol_su_RPB10.
IPR020789. RNA_pol_suN_Zn-BS.
IPR000268. RNAP_N/Rpb10.
[Graphical view]
PfamiPF01194. RNA_pol_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005653. RNA_pol_N/8_sub. 1 hit.
ProDomiPD006539. RNA_pol_N/8_sub. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46924. SSF46924. 1 hit.
PROSITEiPS01112. RNA_POL_N_8KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62875-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL
60
AHVDLIEKLL NYAPLEK
Length:67
Mass (Da):7,645
Last modified:August 16, 2004 - v1
Checksum:i9E8A72F667FE7EC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37690 mRNA. Translation: AAA91459.1.
CR536565 mRNA. Translation: CAG38802.1.
AK311997 mRNA. Translation: BAG34935.1.
BC005903 mRNA. Translation: AAH05903.1.
BC018649 mRNA. Translation: AAH18649.1.
Z47729 Genomic DNA. Translation: CAA87658.1.
Z47728 Genomic DNA. Translation: CAA87657.1.
CCDSiCCDS7720.1.
PIRiS53015.
RefSeqiNP_066951.1. NM_021128.4.
UniGeneiHs.441072.

Genome annotation databases

EnsembliENST00000322028; ENSP00000324124; ENSG00000177700.
ENST00000534030; ENSP00000432807; ENSG00000177700.
GeneIDi5441.
KEGGihsa:5441.
UCSCiuc001lsc.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37690 mRNA. Translation: AAA91459.1.
CR536565 mRNA. Translation: CAG38802.1.
AK311997 mRNA. Translation: BAG34935.1.
BC005903 mRNA. Translation: AAH05903.1.
BC018649 mRNA. Translation: AAH18649.1.
Z47729 Genomic DNA. Translation: CAA87658.1.
Z47728 Genomic DNA. Translation: CAA87657.1.
CCDSiCCDS7720.1.
PIRiS53015.
RefSeqiNP_066951.1. NM_021128.4.
UniGeneiHs.441072.

3D structure databases

ProteinModelPortaliP62875.
SMRiP62875. Positions 1-67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111437. 47 interactions.
IntActiP62875. 21 interactions.
MINTiMINT-1132504.
STRINGi9606.ENSP00000324124.

PTM databases

PhosphoSiteiP62875.

Polymorphism and mutation databases

BioMutaiPOLR2L.
DMDMi51338657.

Proteomic databases

MaxQBiP62875.
PaxDbiP62875.
PRIDEiP62875.

Protocols and materials databases

DNASUi5441.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322028; ENSP00000324124; ENSG00000177700.
ENST00000534030; ENSP00000432807; ENSG00000177700.
GeneIDi5441.
KEGGihsa:5441.
UCSCiuc001lsc.3. human.

Organism-specific databases

CTDi5441.
GeneCardsiGC11M000829.
HGNCiHGNC:9199. POLR2L.
MIMi601189. gene.
neXtProtiNX_P62875.
PharmGKBiPA33519.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1644.
GeneTreeiENSGT00390000007087.
HOVERGENiHBG061475.
InParanoidiP62875.
KOiK03007.
OMAiKKYCCRR.
OrthoDBiEOG79CZ2Z.
PhylomeDBiP62875.
TreeFamiTF103046.

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_1074. RNA Polymerase I Transcription Termination.
REACT_118823. Cytosolic sensors of pathogen-associated DNA.
REACT_12417. MicroRNA (miRNA) biogenesis.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1470. mRNA Capping.
REACT_1628. Transcription-coupled NER (TC-NER).
REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1753. mRNA Splicing - Minor Pathway.
REACT_1845. Formation of RNA Pol II elongation complex.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_1913. RNA Polymerase I Promoter Escape.
REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_2204. RNA Polymerase I Chain Elongation.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
REACT_2222. Dual incision reaction in TC-NER.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_263856. Transcriptional regulation of pluripotent stem cells.
REACT_263965. NoRC negatively regulates rRNA expression.
REACT_264617. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.
REACT_267790. PIWI-interacting RNA (piRNA) biogenesis.
REACT_268530. Transcriptional regulation by small RNAs.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_467. mRNA Splicing - Major Pathway.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
REACT_6233. Transcription of the HIV genome.
REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_6244. Pausing and recovery of HIV elongation.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6259. HIV elongation arrest and recovery.
REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
REACT_63. RNA Polymerase III Transcription Termination.
REACT_6319. Formation of the HIV-1 Early Elongation Complex.
REACT_6332. HIV Transcription Initiation.
REACT_6344. Tat-mediated HIV elongation arrest and recovery.
REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_756. RNA Polymerase III Chain Elongation.
REACT_833. RNA Polymerase II Transcription Elongation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_846. Formation of the Early Elongation Complex.
REACT_953. RNA Polymerase I Transcription Initiation.
REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

GeneWikiiPOLR2L.
GenomeRNAii5441.
NextBioi21055.
PROiP62875.
SOURCEiSearch...

Gene expression databases

BgeeiP62875.
CleanExiHS_POLR2L.
GenevisibleiP62875. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
HAMAPiMF_00250. RNApol_arch_N.
InterProiIPR009057. Homeodomain-like.
IPR023580. RNA_pol_su_RPB10.
IPR020789. RNA_pol_suN_Zn-BS.
IPR000268. RNAP_N/Rpb10.
[Graphical view]
PfamiPF01194. RNA_pol_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005653. RNA_pol_N/8_sub. 1 hit.
ProDomiPD006539. RNA_pol_N/8_sub. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46924. SSF46924. 1 hit.
PROSITEiPS01112. RNA_POL_N_8KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Six human RNA polymerase subunits functionally substitute for their yeast counterparts."
    McKune K., Moore P.A., Hull M.W., Woychik N.A.
    Mol. Cell. Biol. 15:6895-6900(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow and Kidney.
  5. "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
    Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
    Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
  6. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
    Kershnar E., Wu S.-Y., Chiang C.-M.
    J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
  7. "RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor."
    Panov K.I., Panova T.B., Gadal O., Nishiyama K., Saito T., Russell J., Zomerdijk J.C.B.M.
    Mol. Cell. Biol. 26:5436-5448(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRPAB5_HUMAN
AccessioniPrimary (citable) accession number: P62875
Secondary accession number(s): P52436, Q6FHX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.