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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Gene

Gnb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (By similarity).By similarity

GO - Molecular functioni

  1. GTPase activity Source: MGI
  2. GTPase binding Source: MGI
  3. protein complex binding Source: MGI
  4. signal transducer activity Source: UniProtKB-KW
  5. spectrin binding Source: MGI

GO - Biological processi

  1. cardiac muscle cell apoptotic process Source: MGI
  2. cell proliferation Source: MGI
  3. cellular response to hypoxia Source: MGI
  4. G-protein coupled receptor signaling pathway Source: MGI
  5. metabolic process Source: GOC
  6. phospholipase C-activating G-protein coupled receptor signaling pathway Source: MGI
  7. phototransduction, visible light Source: MGI
  8. positive regulation of cytosolic calcium ion concentration Source: Ensembl
  9. retina development in camera-type eye Source: MGI
  10. sensory perception of taste Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiREACT_272374. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_275222. Thrombin signalling through proteinase activated receptors (PARs).
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_291272. G beta:gamma signalling through PI3Kgamma.
REACT_293887. Prostacyclin signalling through prostacyclin receptor.
REACT_296380. G alpha (z) signalling events.
REACT_297430. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_299052. G alpha (12/13) signalling events.
REACT_303206. G-protein activation.
REACT_308732. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_311545. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_313192. G alpha (s) signalling events.
REACT_325400. Olfactory Signaling Pathway.
REACT_326042. Activation of the phototransduction cascade.
REACT_326624. Presynaptic function of Kainate receptors.
REACT_330925. ADP signalling through P2Y purinoceptor 12.
REACT_331048. G alpha (i) signalling events.
REACT_331940. Ca2+ pathway.
REACT_334244. ADP signalling through P2Y purinoceptor 1.
REACT_336725. Thromboxane signalling through TP receptor.
REACT_339334. Activation of G protein gated Potassium channels.
REACT_339920. Glucagon-type ligand receptors.
REACT_345203. Glucagon signaling in metabolic regulation.
REACT_347934. G alpha (q) signalling events.
REACT_351801. G beta:gamma signalling through PLC beta.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Alternative name(s):
Transducin beta chain 1
Gene namesi
Name:Gnb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95781. Gnb1.

Subcellular locationi

GO - Cellular componenti

  1. cell body Source: Ensembl
  2. dendrite Source: Ensembl
  3. extracellular vesicular exosome Source: MGI
  4. heterotrimeric G-protein complex Source: MGI
  5. lysosomal membrane Source: MGI
  6. myelin sheath Source: UniProtKB
  7. photoreceptor inner segment Source: Ensembl
  8. photoreceptor outer segment Source: MGI
  9. photoreceptor outer segment membrane Source: Ensembl
  10. plasma membrane Source: Reactome
  11. vesicle Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1PRO_0000127688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei266 – 2661PhosphohistidineBy similarity

Post-translational modificationi

Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP62874.
PaxDbiP62874.
PRIDEiP62874.

PTM databases

PhosphoSiteiP62874.

Expressioni

Gene expression databases

BgeeiP62874.
ExpressionAtlasiP62874. baseline and differential.
GenevestigatoriP62874.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) (By similarity).By similarity

Protein-protein interaction databases

BioGridi199976. 15 interactions.
IntActiP62874. 8 interactions.
MINTiMINT-248027.

Structurei

3D structure databases

ProteinModelPortaliP62874.
SMRiP62874. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 8331WD 1Add
BLAST
Repeati95 – 12531WD 2Add
BLAST
Repeati141 – 17030WD 3Add
BLAST
Repeati182 – 21231WD 4Add
BLAST
Repeati224 – 25431WD 5Add
BLAST
Repeati268 – 29831WD 6Add
BLAST
Repeati310 – 34031WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62874.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG7GN2N5.
PhylomeDBiP62874.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR
160 170 180 190 200
FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV
210 220 230 240 250
SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC
260 270 280 290 300
RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL
310 320 330 340
KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,377
Last modified:January 23, 2007 - v3
Checksum:i896CBD32D2686598
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29055 mRNA. Translation: AAC52905.1.
AB042854 Genomic DNA. Translation: BAB63904.1.
BC013058 mRNA. Translation: AAH13058.1.
CCDSiCCDS19030.1.
PIRiJC5057.
RefSeqiNP_001153488.1. NM_001160016.1.
NP_001153489.1. NM_001160017.1.
NP_032168.1. NM_008142.4.
UniGeneiMm.2344.

Genome annotation databases

EnsembliENSMUST00000030940; ENSMUSP00000030940; ENSMUSG00000029064.
ENSMUST00000105616; ENSMUSP00000101241; ENSMUSG00000029064.
ENSMUST00000165335; ENSMUSP00000130123; ENSMUSG00000029064.
ENSMUST00000176637; ENSMUSP00000135091; ENSMUSG00000029064.
GeneIDi14688.
KEGGimmu:14688.
UCSCiuc008wdp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29055 mRNA. Translation: AAC52905.1.
AB042854 Genomic DNA. Translation: BAB63904.1.
BC013058 mRNA. Translation: AAH13058.1.
CCDSiCCDS19030.1.
PIRiJC5057.
RefSeqiNP_001153488.1. NM_001160016.1.
NP_001153489.1. NM_001160017.1.
NP_032168.1. NM_008142.4.
UniGeneiMm.2344.

3D structure databases

ProteinModelPortaliP62874.
SMRiP62874. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199976. 15 interactions.
IntActiP62874. 8 interactions.
MINTiMINT-248027.

PTM databases

PhosphoSiteiP62874.

Proteomic databases

MaxQBiP62874.
PaxDbiP62874.
PRIDEiP62874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030940; ENSMUSP00000030940; ENSMUSG00000029064.
ENSMUST00000105616; ENSMUSP00000101241; ENSMUSG00000029064.
ENSMUST00000165335; ENSMUSP00000130123; ENSMUSG00000029064.
ENSMUST00000176637; ENSMUSP00000135091; ENSMUSG00000029064.
GeneIDi14688.
KEGGimmu:14688.
UCSCiuc008wdp.2. mouse.

Organism-specific databases

CTDi2782.
MGIiMGI:95781. Gnb1.

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP62874.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG7GN2N5.
PhylomeDBiP62874.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiREACT_272374. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_275222. Thrombin signalling through proteinase activated receptors (PARs).
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_291272. G beta:gamma signalling through PI3Kgamma.
REACT_293887. Prostacyclin signalling through prostacyclin receptor.
REACT_296380. G alpha (z) signalling events.
REACT_297430. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_299052. G alpha (12/13) signalling events.
REACT_303206. G-protein activation.
REACT_308732. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_311545. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_313192. G alpha (s) signalling events.
REACT_325400. Olfactory Signaling Pathway.
REACT_326042. Activation of the phototransduction cascade.
REACT_326624. Presynaptic function of Kainate receptors.
REACT_330925. ADP signalling through P2Y purinoceptor 12.
REACT_331048. G alpha (i) signalling events.
REACT_331940. Ca2+ pathway.
REACT_334244. ADP signalling through P2Y purinoceptor 1.
REACT_336725. Thromboxane signalling through TP receptor.
REACT_339334. Activation of G protein gated Potassium channels.
REACT_339920. Glucagon-type ligand receptors.
REACT_345203. Glucagon signaling in metabolic regulation.
REACT_347934. G alpha (q) signalling events.
REACT_351801. G beta:gamma signalling through PLC beta.

Miscellaneous databases

ChiTaRSiGnb1. mouse.
NextBioi286631.
PROiP62874.
SOURCEiSearch...

Gene expression databases

BgeeiP62874.
ExpressionAtlasiP62874. baseline and differential.
GenevestigatoriP62874.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Qiu R., Schimmer B.P.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.
  2. "Genomic organization of the murine G protein beta subunit genes and related processed pseudogenes."
    Kitanaka J., Wang X., Kitanaka N., Hembree C.M., Uhl G.R.
    DNA Seq. 12:345-354(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Lubec G., Klug S., Kang S.U.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 24-42; 58-96; 138-150; 198-209 AND 284-301, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiGBB1_MOUSE
AccessioniPrimary (citable) accession number: P62874
Secondary accession number(s): P04697, P04901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.