P62874 (GBB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Alternative name(s):
Transducin beta chain 1

Gene names

Name:

Gnb1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction By similarity.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.
Post-translational modification	Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP By similarity.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
Molecular function	Transducer
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle cell apoptotic process Inferred from sequence orthology PubMed 19723622. Source: MGI cell proliferation Inferred from direct assay PubMed 14712229. Source: MGI cellular response to hypoxia Inferred from sequence orthology PubMed 19723622. Source: MGI elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 10570481. Source: MGI phototransduction, visible light Inferred from sequence alignment PubMed 2328987. Source: MGI retina development in camera-type eye Inferred from mutant phenotype PubMed 21052544. Source: MGI sensory perception of taste Inferred from direct assay PubMed 10570481. Source: MGI
Cellular_component	cell body Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara heterotrimeric G-protein complex Inferred from physical interaction PubMed 10570481. Source: MGI photoreceptor inner segment Inferred from electronic annotation. Source: Compara photoreceptor outer segment Inferred from direct assay PubMed 18367617. Source: MGI photoreceptor outer segment membrane Inferred from electronic annotation. Source: Compara
Molecular_function	GTPase activity Inferred from sequence alignment PubMed 1543505. Source: MGI signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 340	339	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1		PRO_0000127688
Regions
	Repeat	53 – 83	31	WD 1
	Repeat	95 – 125	31	WD 2
	Repeat	141 – 170	30	WD 3
	Repeat	182 – 212	31	WD 4
	Repeat	224 – 254	31	WD 5
	Repeat	268 – 298	31	WD 6
	Repeat	310 – 340	31	WD 7
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	266	1	Phosphohistidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62874 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 896CBD32D2686598	FASTA	340	37,377
	10 20 30 40 50 60 MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA 70 80 90 100 110 120 MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI 130 140 150 160 170 180 CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF 190 200 210 220 230 240 TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA 250 260 270 280 290 300 FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 310 320 330 340 KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Qiu R., Schimmer B.P. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[2]	"Genomic organization of the murine G protein beta subunit genes and related processed pseudogenes." Kitanaka J., Wang X., Kitanaka N., Hembree C.M., Uhl G.R. DNA Seq. 12:345-354(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Lubec G., Klug S., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 24-42; 58-96; 138-150; 198-209 AND 284-301, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29055 mRNA. Translation: AAC52905.1. AB042854 Genomic DNA. Translation: BAB63904.1. BC013058 mRNA. Translation: AAH13058.1.
IPI	IPI00120716.
PIR	JC5057.
RefSeq	NP_001153488.1. NM_001160016.1. NP_001153489.1. NM_001160017.1. NP_032168.1. NM_008142.4.
UniGene	Mm.2344.
3D structure databases
ProteinModelPortal	P62874.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62874. 4 interactions.
PTM databases
PhosphoSite	P62874.
Proteomic databases
PaxDb	P62874.
PRIDE	P62874.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030940; ENSMUSP00000030940; ENSMUSG00000029064. ENSMUST00000105616; ENSMUSP00000101241; ENSMUSG00000029064. ENSMUST00000165335; ENSMUSP00000130123; ENSMUSG00000029064. ENSMUST00000176637; ENSMUSP00000135091; ENSMUSG00000029064.
GeneID	14688.
KEGG	mmu:14688.
Organism-specific databases
CTD	2782.
MGI	MGI:95781. Gnb1.
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000176356.
HOVERGEN	HBG000188.
InParanoid	P62874.
KO	K04536.
OMA	CMLWDIE.
OrthoDB	EOG4HX51D.
Gene expression databases
ArrayExpress	P62874.
Bgee	P62874.
Genevestigator	P62874.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR001632. Gprotein_B. IPR016346. Guanine_nucleotide-bd_bsu. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 7 hits. [Graphical view]
PIRSF	PIRSF002394. GN-bd_beta. 1 hit.
PRINTS	PR00319. GPROTEINB. PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GNB1. mouse.
NextBio	286631.
SOURCE	Search...

Entry information

Entry name

GBB1_MOUSE

Accession

Primary (citable) accession number: P62874
Secondary accession number(s): P04697, P04901

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program