Reviewed, UniProtKB/Swiss-Prot P62873 (GBB1_HUMAN)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Alternative name(s):
    Transducin beta chain 1

Gene names

Name:

GNB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18. Ref.11
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
Molecular function	Transducer
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	Ras protein signal transduction Traceable author statement. Source: ProtInc hormone-mediated signaling Inferred from Experiment. Source: Reactome muscarinic acetylcholine receptor signaling pathway Traceable author statement. Source: ProtInc
Molecular function	GTPase activity Ref.1 Traceable author statement. Source: ProtInc signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.8 Ref.9

Chain

2 – 340

339

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

PRO_0000127687

Regions

Repeat

53 – 83

31

WD 1

Repeat

95 – 125

31

WD 2

Repeat

141 – 170

30

WD 3

Repeat

182 – 212

31

WD 4

Repeat

224 – 254

31

WD 5

Repeat

268 – 298

31

WD 6

Repeat

310 – 340

31

WD 7

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.8 Ref.9

Secondary structure

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340

Helix Strand Turn

Details...

Turn

11 – 15

5

Helix

16 – 19

4

Helix

20 – 22

3

Turn

31 – 34

4

Beta strand

35 – 37

3

Beta strand

47 – 51

5

Beta strand

60 – 63

4

Beta strand

67 – 73

7

Beta strand

79 – 83

5

Turn

84 – 86

3

Beta strand

89 – 92

4

Beta strand

100 – 105

6

Beta strand

109 – 118

10

Beta strand

121 – 125

5

Beta strand

135 – 139

5

Beta strand

148 – 153

6

Beta strand

156 – 160

5

Beta strand

162 – 164

3

Beta strand

166 – 170

5

Turn

171 – 174

4

Beta strand

175 – 179

5

Beta strand

187 – 192

6

Beta strand

194 – 203

10

Beta strand

208 – 212

5

Turn

213 – 215

3

Beta strand

238 – 242

5

Beta strand

245 – 248

4

Beta strand

252 – 254

3

Turn

255 – 258

4

Beta strand

259 – 262

4

Beta strand

276 – 278

3

Beta strand

282 – 288

7

Beta strand

290 – 298

9

Turn

299 – 301

3

Beta strand

315 – 320

6

Beta strand

327 – 331

5

Beta strand

336 – 339

4

Sequences

	Sequence		Length	Mass (Da)
	P62873-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 896CBD32D2686598	FASTA	340	37,377
	10 20 30 40 50 60 MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA 70 80 90 100 110 120 MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI 130 140 150 160 170 180 CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF 190 200 210 220 230 240 TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA 250 260 270 280 290 300 FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 310 320 330 340 KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical." Codina J., Stengel D., Woo S.L.C., Birnbaumer L. FEBS Lett. 207:187-192(1986) [PubMed: 3095147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Muscle and Uterus.
[8]	Bienvenut W.V., Quadroni M. Submitted (JUL-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15; 58-89; 284-301 AND 305-314, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma and Melanoma.
[9]	Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-8; 69-78 AND 90-96, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[10]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-42; 58-78; 138-150; 198-209 AND 315-337, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[11]	"G Protein betagamma subunits stimulate p114RhoGEF, a guanine nucleotide exchange factor for RhoA and Rac1: regulation of cell shape and reactive oxygen species production." Niu J., Profirovic J., Pan H., Vaiskunaite R., Voyno-Yasenetskaya T. Circ. Res. 93:848-856(2003) [PubMed: 14512443] [Abstract] Cited for: INTERACTION WITH ARHGEF18.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X04526 mRNA. Translation: CAA28207.1.
AF501882 mRNA. Translation: AAM15918.1.
BT007305 mRNA. Translation: AAP35969.1.
CR456784 mRNA. Translation: CAG33065.1.
AL031282, AL109917 Genomic DNA. Translation: CAI20029.1.
AL109917, AL031282 Genomic DNA. Translation: CAI95654.1.
CH471183 Genomic DNA. Translation: EAW56147.1.
BC004186 mRNA. Translation: AAH04186.1.
BC005888 mRNA. Translation: AAH05888.1.
BC008991 mRNA. Translation: AAH08991.1.
M36430 mRNA. Translation: AAA63265.1.

IPI

IPI00026268.

PIR

RGHUB1. A24853.

RefSeq

NP_002065.1.

UniGene

Hs.430425
Hs.709208

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QNS	X-ray	3.00	A	1-340	[»]

SMR

P62873. Positions 1-339, 2-340.

ModBase

Search...

Protein-protein interaction databases

IntAct

P62873. 5 interactions.

2-D gel databases

OGP

P62873.

REPRODUCTION-2DPAGE

IPI00026268.

Proteomic databases

PRIDE

P62873.

Genome annotation databases

Ensembl

ENSG00000078369. Homo sapiens. [Contig view]

GeneID

2782.

KEGG

hsa:2782.

Organism-specific databases

GeneCards

GC01M001748.

HGNC

HGNC:4396. GNB1.

MIM

139380. gene.

PharmGKB

PA28776.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P62873.

HOVERGEN

P62873.

OMA

P62873. NQIREAR.

Enzyme and pathway databases

Pathway_Interaction_DB

hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins.
lysophospholipid_pathway. LPA receptor mediated events.
wnt_calcium_pathway. Noncanonical Wnt signaling pathway.
er_nongenomic_pathway. Plasma membrane estrogen receptor signaling.
hdac_classii_pathway. Signaling events mediated by HDAC Class II.
txa2pathway. Thromboxane A2 receptor signaling.
rhodopsin_pathway. Visual signal transduction: Rods.

Reactome

REACT_1505. Integration of energy metabolism.

Gene expression databases

ArrayExpress

P62873.

Bgee

P62873.

CleanEx

HS_GNB1.

GermOnline

ENSG00000078369. Homo sapiens.

Family and domain databases

InterPro

IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like.
IPR001680. WD40_repeat.
IPR019782. WD40_repeat_2.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_region.
IPR019781. WD40_repeat_sg.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

Pfam

PF00400. WD40. 7 hits.
[Graphical view]

PIRSF

PIRSF002394. GN-bd_beta. 1 hit.

PRINTS

PR00319. GPROTEINB.

ProDom

PD000018. WD40. 5 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00320. WD40. 7 hits.
[Graphical view]

PROSITE

PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

10959.

SOURCE

Search...

Entry information

Entry name

GBB1_HUMAN

Accession

Primary (citable) accession number: P62873
Secondary accession number(s): B1AJZ7, P04697, P04901

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)