P62873 (GBB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Alternative name(s):
Transducin beta chain 1

Gene names

Name:

GNB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Ref.14
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus). Ref.11 Ref.12 Ref.14
Post-translational modification	Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP By similarity.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
Molecular function	Transducer
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	G-protein coupled acetylcholine receptor signaling pathway Traceable author statement. Source: ProtInc Ras protein signal transduction Traceable author statement. Source: ProtInc cellular response to glucagon stimulus Traceable author statement. Source: Reactome energy reserve metabolic process Traceable author statement. Source: Reactome platelet activation Traceable author statement. Source: Reactome synaptic transmission Traceable author statement. Source: Reactome
Cellular component	heterotrimeric G-protein complex Inferred from direct assay. Source: MGI
Molecular function	GTPase activity Inferred from direct assay. Source: MGI GTPase binding Inferred from physical interaction Ref.12. Source: UniProtKB signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.8 Ref.9

Chain

2 – 340

339

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

PRO_0000127687

Regions

Repeat

53 – 83

31

WD 1

Repeat

95 – 125

31

WD 2

Repeat

141 – 170

30

WD 3

Repeat

182 – 212

31

WD 4

Repeat

224 – 254

31

WD 5

Repeat

268 – 298

31

WD 6

Repeat

310 – 340

31

WD 7

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.8 Ref.9

Modified residue

266

1

Phosphohistidine By similarity

Experimental info

Mutagenesis

47

1

T → A: Abolishes interaction with PTH1R and signaling in response to PTH1R agonist; when associated with A-337 and A-339. Ref.14

Mutagenesis

337

1

K → A: Abolishes interaction with PTH1R and signaling in response to PTH1R agonist; when associated with A-47 and A-339. Ref.14

Mutagenesis

339

1

W → A: Abolishes interaction with PTH1R and signaling in response to PTH1R agonist; when associated with A-47 and A-337. Ref.14

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

340

Helix Strand Turn

Details...

Turn

11 – 15

5

Helix

16 – 19

4

Helix

20 – 22

3

Turn

31 – 34

4

Beta strand

35 – 37

3

Beta strand

47 – 51

5

Beta strand

60 – 63

4

Beta strand

67 – 73

7

Beta strand

79 – 83

5

Turn

84 – 86

3

Beta strand

89 – 92

4

Beta strand

100 – 105

6

Beta strand

109 – 118

10

Beta strand

121 – 125

5

Beta strand

135 – 139

5

Beta strand

148 – 153

6

Beta strand

156 – 160

5

Beta strand

162 – 164

3

Beta strand

166 – 170

5

Turn

171 – 174

4

Beta strand

175 – 179

5

Beta strand

187 – 192

6

Beta strand

194 – 203

10

Beta strand

208 – 212

5

Turn

213 – 215

3

Beta strand

238 – 242

5

Beta strand

245 – 248

4

Beta strand

252 – 254

3

Turn

255 – 258

4

Beta strand

259 – 262

4

Beta strand

276 – 278

3

Beta strand

282 – 288

7

Beta strand

290 – 298

9

Turn

299 – 301

3

Beta strand

315 – 320

6

Beta strand

327 – 331

5

Beta strand

336 – 339

4

Sequences

	Sequence		Length	Mass (Da)
	P62873 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 896CBD32D2686598	FASTA	340	37,377
	10 20 30 40 50 60 MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA 70 80 90 100 110 120 MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI 130 140 150 160 170 180 CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF 190 200 210 220 230 240 TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA 250 260 270 280 290 300 FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 310 320 330 340 KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical." Codina J., Stengel D., Woo S.L.C., Birnbaumer L. FEBS Lett. 207:187-192(1986) [PubMed: 3095147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Muscle and Uterus.
[8]	Bienvenut W.V., Quadroni M. Submitted (JUL-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15; 58-89; 284-301 AND 305-314, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma and Melanoma.
[9]	Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-8; 69-78 AND 90-96, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[10]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-42; 58-78; 138-150; 198-209 AND 315-337, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[11]	"G Protein betagamma subunits stimulate p114RhoGEF, a guanine nucleotide exchange factor for RhoA and Rac1: regulation of cell shape and reactive oxygen species production." Niu J., Profirovic J., Pan H., Vaiskunaite R., Voyno-Yasenetskaya T. Circ. Res. 93:848-856(2003) [PubMed: 14512443] [Abstract] Cited for: INTERACTION WITH ARHGEF18.
[12]	"The cationic region of Rhes mediates its interactions with specific Gbeta subunits." Hill C., Goddard A., Ladds G., Davey J. Cell. Physiol. Biochem. 23:1-8(2009) [PubMed: 19255495] [Abstract] Cited for: INTERACTION WITH RASD2.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"Structure of the parathyroid hormone receptor C terminus bound to the G-protein dimer Gbeta1gamma2." Johnston C.A., Kimple A.J., Giguere P.M., Siderovski D.P. Structure 16:1086-1094(2008) [PubMed: 18611381] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEXES WITH GNG2 AND PTH1R, FUNCTION, SUBUNIT, MUTAGENESIS OF THR-47; LYS-337 AND TRP-339.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04526 mRNA. Translation: CAA28207.1.
AF501882 mRNA. Translation: AAM15918.1.
BT007305 mRNA. Translation: AAP35969.1.
CR456784 mRNA. Translation: CAG33065.1.
AL031282, AL109917 Genomic DNA. Translation: CAI20029.1.
AL109917, AL031282 Genomic DNA. Translation: CAI95654.1.
CH471183 Genomic DNA. Translation: EAW56147.1.
BC004186 mRNA. Translation: AAH04186.1.
BC005888 mRNA. Translation: AAH05888.1.
BC008991 mRNA. Translation: AAH08991.1.
M36430 mRNA. Translation: AAA63265.1.

IPI

IPI00026268.

PIR

RGHUB1. A24853.

RefSeq

NP_002065.1. NM_002074.3.

UniGene

Hs.430425.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QNS	X-ray	3.00	A	1-340	[»]
3KJ5	X-ray	3.00	A	1-340	[»]

ProteinModelPortal

P62873.

SMR

P62873. Positions 1-340.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-599N.

IntAct

P62873. 15 interactions.

MINT

MINT-94562.

STRING

P62873.

PTM databases

PhosphoSite

P62873.

Polymorphism databases

DMDM

51317302.

2D gel databases

OGP

P62873.

REPRODUCTION-2DPAGE

IPI00026268.

Proteomic databases

PRIDE

P62873.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000378609; ENSP00000367872; ENSG00000078369.
ENST00000455156; ENSP00000416870; ENSG00000078369.

GeneID

2782.

KEGG

hsa:2782.

UCSC

uc001aif.1. human.

Organism-specific databases

CTD

2782.

GeneCards

GC01M001748.

H-InvDB

HIX0000041.

HGNC

HGNC:4396. GNB1.

MIM

139380. gene.

neXtProt

NX_P62873.

PharmGKB

PA28776.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG12689.

HOGENOM

HBG396231.

HOVERGEN

HBG000188.

InParanoid

P62873.

OMA

CMLWDIE.

OrthoDB

EOG4HX51D.

PhylomeDB

P62873.

Enzyme and pathway databases

Pathway_Interaction_DB

hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins.
lysophospholipid_pathway. LPA receptor mediated events.
wnt_calcium_pathway. Noncanonical Wnt signaling pathway.
er_nongenomic_pathway. Plasma membrane estrogen receptor signaling.
hdac_classii_pathway. Signaling events mediated by HDAC Class II.
txa2pathway. Thromboxane A2 receptor signaling.
rhodopsin_pathway. Visual signal transduction: Rods.

Reactome

REACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpress

P62873.

Bgee

P62873.

CleanEx

HS_GNB1.

Genevestigator

P62873.

GermOnline

ENSG00000078369. Homo sapiens.

Family and domain databases

InterPro

IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

KO

K04536.

Pfam

PF00400. WD40. 7 hits.
[Graphical view]

PIRSF

PIRSF002394. GN-bd_beta. 1 hit.

PRINTS

PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

10959.

SOURCE

Search...

Entry information

Entry name

GBB1_HUMAN

Accession

Primary (citable) accession number: P62873
Secondary accession number(s): B1AJZ7, P04697, P04901

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.