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P62871

- GBB1_BOVIN

UniProt

P62871 - GBB1_BOVIN

Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Gene

GNB1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. GTPase activity Source: Ensembl
    2. protein binding Source: IntAct
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. adenylate cyclase-activating dopamine receptor signaling pathway Source: BHF-UCL
    2. cell proliferation Source: Ensembl
    3. cellular response to catecholamine stimulus Source: BHF-UCL
    4. cellular response to prostaglandin E stimulus Source: BHF-UCL
    5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
    6. retina development in camera-type eye Source: Ensembl
    7. sensory perception of taste Source: Ensembl

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_202137. Regulation of water balance by renal Aquaporins.
    REACT_202218. G beta:gamma signalling through PI3Kgamma.
    REACT_205384. G alpha (12/13) signalling events.
    REACT_206547. G alpha (s) signalling events.
    REACT_208538. Activation of G protein gated Potassium channels.
    REACT_209033. G alpha (z) signalling events.
    REACT_209198. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_209521. G alpha (q) signalling events.
    REACT_209727. Ca2+ pathway.
    REACT_210152. Thromboxane signalling through TP receptor.
    REACT_210568. G alpha (i) signalling events.
    REACT_211545. ADP signalling through P2Y purinoceptor 1.
    REACT_211562. Activation of the phototransduction cascade.
    REACT_215840. Olfactory Signaling Pathway.
    REACT_215912. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_217228. Presynaptic function of Kainate receptors.
    REACT_217846. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_218219. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_219437. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_219794. ADP signalling through P2Y purinoceptor 12.
    REACT_220607. Glucagon-type ligand receptors.
    REACT_221707. Glucagon signaling in metabolic regulation.
    REACT_222419. G-protein activation.
    REACT_224596. G beta:gamma signalling through PLC beta.
    REACT_227415. Prostacyclin signalling through prostacyclin receptor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Alternative name(s):
    Transducin beta chain 1
    Gene namesi
    Name:GNB1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    GO - Cellular componenti

    1. heterotrimeric G-protein complex Source: BHF-UCL
    2. intracellular Source: BHF-UCL
    3. lysosomal membrane Source: Ensembl
    4. membrane Source: BHF-UCL
    5. photoreceptor disc membrane Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1PRO_0000127684Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei266 – 2661Phosphohistidine1 Publication

    Post-translational modificationi

    Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP62871.
    PRIDEiP62871.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NME2Q3T0Q43EBI-357141,EBI-8577979

    Protein-protein interaction databases

    BioGridi158560. 5 interactions.
    DIPiDIP-29227N.
    IntActiP62871. 5 interactions.
    MINTiMINT-1864875.

    Structurei

    Secondary structure

    1
    340
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 2422
    Helixi30 – 334
    Turni34 – 363
    Beta strandi47 – 515
    Beta strandi58 – 636
    Beta strandi67 – 748
    Turni75 – 773
    Beta strandi78 – 836
    Turni84 – 863
    Beta strandi89 – 946
    Beta strandi96 – 983
    Beta strandi100 – 1056
    Beta strandi109 – 1168
    Beta strandi120 – 1256
    Turni126 – 1283
    Beta strandi130 – 1323
    Beta strandi134 – 1407
    Beta strandi146 – 1538
    Beta strandi156 – 1616
    Beta strandi166 – 1705
    Turni171 – 1744
    Beta strandi175 – 1806
    Beta strandi187 – 1926
    Beta strandi196 – 2038
    Beta strandi208 – 2125
    Turni213 – 2153
    Beta strandi217 – 2226
    Beta strandi229 – 2346
    Beta strandi238 – 2458
    Beta strandi250 – 2545
    Turni255 – 2584
    Beta strandi259 – 2646
    Beta strandi273 – 2786
    Beta strandi282 – 2898
    Beta strandi292 – 2987
    Turni299 – 3013
    Beta strandi304 – 3096
    Beta strandi315 – 3206
    Beta strandi327 – 3315
    Beta strandi336 – 3394

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A0RX-ray2.80B2-340[»]
    1B9XX-ray3.00A1-340[»]
    1B9YX-ray3.00A1-340[»]
    1GG2X-ray2.40B1-340[»]
    1GOTX-ray2.00B1-340[»]
    1GP2X-ray2.30B1-340[»]
    1OMWX-ray2.50B1-340[»]
    1TBGX-ray2.10A/B/C/D1-340[»]
    1XHMX-ray2.70A1-340[»]
    2BCJX-ray3.06B2-340[»]
    2TRCX-ray2.40B1-340[»]
    3AH8X-ray2.90B1-340[»]
    3CIKX-ray2.75B1-340[»]
    3KRWX-ray2.90B1-340[»]
    3KRXX-ray3.10B1-340[»]
    3PSCX-ray2.67B1-340[»]
    3PVUX-ray2.48B1-340[»]
    3PVWX-ray2.49B1-340[»]
    3UZSX-ray4.52B1-340[»]
    3V5WX-ray2.07B1-340[»]
    4MK0X-ray2.40B2-340[»]
    ProteinModelPortaliP62871.
    SMRiP62871. Positions 2-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62871.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati53 – 8331WD 1Add
    BLAST
    Repeati95 – 12531WD 2Add
    BLAST
    Repeati141 – 17030WD 3Add
    BLAST
    Repeati182 – 21231WD 4Add
    BLAST
    Repeati224 – 25431WD 5Add
    BLAST
    Repeati268 – 29831WD 6Add
    BLAST
    Repeati310 – 34031WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115196.
    HOGENOMiHOG000176356.
    HOVERGENiHBG000188.
    InParanoidiP62871.
    KOiK04536.
    OMAiRIVMRPR.
    OrthoDBiEOG7GN2N5.
    TreeFamiTF106149.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT    50
    LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV 100
    MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR 150
    FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV 200
    SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC 250
    RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 300
    KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN 340
    Length:340
    Mass (Da):37,377
    Last modified:January 23, 2007 - v3
    Checksum:i896CBD32D2686598
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711V → L in CAA26875. (PubMed:2414128)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03073 mRNA. Translation: CAA26875.1.
    M13236 mRNA. Translation: AAA30792.1.
    BC105260 mRNA. Translation: AAI05261.1.
    PIRiA24225. RGBOB1.
    RefSeqiNP_786971.2. NM_175777.3.
    XP_005217074.1. XM_005217017.1.
    UniGeneiBt.57702.

    Genome annotation databases

    EnsembliENSBTAT00000000259; ENSBTAP00000000259; ENSBTAG00000000215.
    ENSBTAT00000045065; ENSBTAP00000042481; ENSBTAG00000000215.
    GeneIDi281201.
    KEGGibta:281201.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03073 mRNA. Translation: CAA26875.1 .
    M13236 mRNA. Translation: AAA30792.1 .
    BC105260 mRNA. Translation: AAI05261.1 .
    PIRi A24225. RGBOB1.
    RefSeqi NP_786971.2. NM_175777.3.
    XP_005217074.1. XM_005217017.1.
    UniGenei Bt.57702.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A0R X-ray 2.80 B 2-340 [» ]
    1B9X X-ray 3.00 A 1-340 [» ]
    1B9Y X-ray 3.00 A 1-340 [» ]
    1GG2 X-ray 2.40 B 1-340 [» ]
    1GOT X-ray 2.00 B 1-340 [» ]
    1GP2 X-ray 2.30 B 1-340 [» ]
    1OMW X-ray 2.50 B 1-340 [» ]
    1TBG X-ray 2.10 A/B/C/D 1-340 [» ]
    1XHM X-ray 2.70 A 1-340 [» ]
    2BCJ X-ray 3.06 B 2-340 [» ]
    2TRC X-ray 2.40 B 1-340 [» ]
    3AH8 X-ray 2.90 B 1-340 [» ]
    3CIK X-ray 2.75 B 1-340 [» ]
    3KRW X-ray 2.90 B 1-340 [» ]
    3KRX X-ray 3.10 B 1-340 [» ]
    3PSC X-ray 2.67 B 1-340 [» ]
    3PVU X-ray 2.48 B 1-340 [» ]
    3PVW X-ray 2.49 B 1-340 [» ]
    3UZS X-ray 4.52 B 1-340 [» ]
    3V5W X-ray 2.07 B 1-340 [» ]
    4MK0 X-ray 2.40 B 2-340 [» ]
    ProteinModelPortali P62871.
    SMRi P62871. Positions 2-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 158560. 5 interactions.
    DIPi DIP-29227N.
    IntActi P62871. 5 interactions.
    MINTi MINT-1864875.

    Proteomic databases

    PaxDbi P62871.
    PRIDEi P62871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000000259 ; ENSBTAP00000000259 ; ENSBTAG00000000215 .
    ENSBTAT00000045065 ; ENSBTAP00000042481 ; ENSBTAG00000000215 .
    GeneIDi 281201.
    KEGGi bta:281201.

    Organism-specific databases

    CTDi 2782.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115196.
    HOGENOMi HOG000176356.
    HOVERGENi HBG000188.
    InParanoidi P62871.
    KOi K04536.
    OMAi RIVMRPR.
    OrthoDBi EOG7GN2N5.
    TreeFami TF106149.

    Enzyme and pathway databases

    Reactomei REACT_202137. Regulation of water balance by renal Aquaporins.
    REACT_202218. G beta:gamma signalling through PI3Kgamma.
    REACT_205384. G alpha (12/13) signalling events.
    REACT_206547. G alpha (s) signalling events.
    REACT_208538. Activation of G protein gated Potassium channels.
    REACT_209033. G alpha (z) signalling events.
    REACT_209198. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_209521. G alpha (q) signalling events.
    REACT_209727. Ca2+ pathway.
    REACT_210152. Thromboxane signalling through TP receptor.
    REACT_210568. G alpha (i) signalling events.
    REACT_211545. ADP signalling through P2Y purinoceptor 1.
    REACT_211562. Activation of the phototransduction cascade.
    REACT_215840. Olfactory Signaling Pathway.
    REACT_215912. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_217228. Presynaptic function of Kainate receptors.
    REACT_217846. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_218219. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_219437. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_219794. ADP signalling through P2Y purinoceptor 12.
    REACT_220607. Glucagon-type ligand receptors.
    REACT_221707. Glucagon signaling in metabolic regulation.
    REACT_222419. G-protein activation.
    REACT_224596. G beta:gamma signalling through PLC beta.
    REACT_227415. Prostacyclin signalling through prostacyclin receptor.

    Miscellaneous databases

    EvolutionaryTracei P62871.
    NextBioi 20805257.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs."
      Fong H.K.W., Hurley J.B., Hopkins R.S., Miake-Lye R., Johnson M.S., Doolittle R.F., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 83:2162-2166(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Primary structure of the beta-subunit of bovine transducin deduced from the cDNA sequence."
      Sugimoto K., Nukada T., Tanabe T., Takahashi H., Noda M., Minamino N., Kangawa K., Matsuo H., Hirose T., Inayama S., Numa S.
      FEBS Lett. 191:235-240(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.
    4. "Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta."
      Cuello F., Schulze R.A., Heemeyer F., Meyer H.E., Lutz S., Jakobs K.H., Niroomand F., Wieland T.
      J. Biol. Chem. 278:7220-7226(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT HIS-266.
    5. "The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2."
      Wall M.A., Coleman D.E., Lee E., Iniguez-Lluhi J.A., Posner B.A., Gilman A.G., Sprang S.R.
      Cell 83:1047-1058(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF HETEROTRIMER.
    6. "Crystal structure of a G-protein beta gamma dimer at 2.1-A resolution."
      Sondek J., Bohm A., Lambright D.G., Hamm H.E., Sigler P.B.
      Nature 379:369-374(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF BETA-GAMMA DIMER.
    7. "Phosducin induces a structural change in transducin beta gamma."
      Loew A., Ho Y.K., Blundell T., Bax B.
      Structure 6:1007-1019(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF COMPLEX WITH PHOSDUCIN.
    8. "Keeping G proteins at bay: a complex between G protein-coupled receptor kinase 2 and Gbetagamma."
      Lodowski D.T., Pitcher J.A., Capel W.D., Lefkowitz R.J., Tesmer J.J.
      Science 300:1256-1262(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF COMPLEX WITH ADRBK1 AND GNG2.

    Entry informationi

    Entry nameiGBB1_BOVIN
    AccessioniPrimary (citable) accession number: P62871
    Secondary accession number(s): P04697, P04901, Q3MHF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3