P62871 (GBB1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Alternative name(s):
Transducin beta chain 1

Gene names

Name:

GNB1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.
Post-translational modification	Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
Molecular function	Transducer
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 340

339

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

PRO_0000127684

Regions

Repeat

53 – 83

31

WD 1

Repeat

95 – 125

31

WD 2

Repeat

141 – 170

30

WD 3

Repeat

182 – 212

31

WD 4

Repeat

224 – 254

31

WD 5

Repeat

268 – 298

31

WD 6

Repeat

310 – 340

31

WD 7

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

266

1

Phosphohistidine Ref.4

Experimental info

Sequence conflict

71

1

V → L in CAA26875. Ref.2

Secondary structure

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340

Helix Strand Turn

Details...

Helix

3 – 25

23

Helix

30 – 34

5

Beta strand

47 – 51

5

Beta strand

58 – 63

6

Beta strand

67 – 74

8

Turn

75 – 77

3

Beta strand

78 – 83

6

Turn

84 – 86

3

Beta strand

89 – 94

6

Beta strand

100 – 105

6

Beta strand

109 – 116

8

Beta strand

120 – 125

6

Beta strand

135 – 140

6

Beta strand

146 – 153

8

Beta strand

156 – 161

6

Beta strand

166 – 170

5

Turn

171 – 173

3

Beta strand

176 – 180

5

Beta strand

187 – 192

6

Beta strand

196 – 203

8

Beta strand

206 – 212

7

Turn

213 – 216

4

Beta strand

217 – 223

7

Beta strand

229 – 234

6

Beta strand

238 – 245

8

Beta strand

250 – 254

5

Turn

255 – 258

4

Beta strand

259 – 264

6

Beta strand

273 – 278

6

Beta strand

284 – 289

6

Beta strand

292 – 298

7

Turn

299 – 301

3

Beta strand

303 – 309

7

Beta strand

315 – 320

6

Beta strand

327 – 331

5

Beta strand

336 – 339

4

Sequences

	Sequence		Length	Mass (Da)
	P62871 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 896CBD32D2686598	FASTA	340	37,377
	10 20 30 40 50 60 MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA 70 80 90 100 110 120 MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI 130 140 150 160 170 180 CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF 190 200 210 220 230 240 TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA 250 260 270 280 290 300 FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 310 320 330 340 KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs." Fong H.K.W., Hurley J.B., Hopkins R.S., Miake-Lye R., Johnson M.S., Doolittle R.F., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 83:2162-2166(1986) [PubMed: 3083416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Primary structure of the beta-subunit of bovine transducin deduced from the cDNA sequence." Sugimoto K., Nukada T., Tanabe T., Takahashi H., Noda M., Minamino N., Kangawa K., Matsuo H., Hirose T., Inayama S., Numa S. FEBS Lett. 191:235-240(1985) [PubMed: 2414128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.
[4]	"Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta." Cuello F., Schulze R.A., Heemeyer F., Meyer H.E., Lutz S., Jakobs K.H., Niroomand F., Wieland T. J. Biol. Chem. 278:7220-7226(2003) [PubMed: 12486123] [Abstract] Cited for: PHOSPHORYLATION AT HIS-266.
[5]	"The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2." Wall M.A., Coleman D.E., Lee E., Iniguez-Lluhi J.A., Posner B.A., Gilman A.G., Sprang S.R. Cell 83:1047-1058(1995) [PubMed: 8521505] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF HETEROTRIMER.
[6]	"Crystal structure of a G-protein beta gamma dimer at 2.1-A resolution." Sondek J., Bohm A., Lambright D.G., Hamm H.E., Sigler P.B. Nature 379:369-374(1996) [PubMed: 8552196] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF BETA-GAMMA DIMER.
[7]	"Phosducin induces a structural change in transducin beta gamma." Loew A., Ho Y.K., Blundell T., Bax B. Structure 6:1007-1019(1998) [PubMed: 9739091] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF COMPLEX WITH PHOSDUCIN.
[8]	"Keeping G proteins at bay: a complex between G protein-coupled receptor kinase 2 and Gbetagamma." Lodowski D.T., Pitcher J.A., Capel W.D., Lefkowitz R.J., Tesmer J.J. Science 300:1256-1262(2003) [PubMed: 12764189] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF COMPLEX WITH ADRBK1 AND GNG2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03073 mRNA. Translation: CAA26875.1.
M13236 mRNA. Translation: AAA30792.1.
BC105260 mRNA. Translation: AAI05261.1.

IPI

IPI00703160.

PIR

RGBOB1. A24225.

RefSeq

NP_786971.2. NM_175777.3.

UniGene

Bt.57702.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A0R	X-ray	2.80	B	2-340	[»]
1B9X	X-ray	3.00	A	1-340	[»]
1B9Y	X-ray	3.00	A	1-340	[»]
1GG2	X-ray	2.40	B	1-340	[»]
1GOT	X-ray	2.00	B	1-340	[»]
1GP2	X-ray	2.30	B	1-340	[»]
1OMW	X-ray	2.50	B	1-340	[»]
1TBG	X-ray	2.10	A/B/C/D	1-340	[»]
1XHM	X-ray	2.70	A	1-340	[»]
2BCJ	X-ray	3.06	B	2-339	[»]
2TRC	X-ray	2.40	B	1-340	[»]
3AH8	X-ray	2.90	B	1-340	[»]
3CIK	X-ray	2.75	B	1-340	[»]
3KRW	X-ray	2.90	B	1-340	[»]
3KRX	X-ray	3.10	B	1-340	[»]
3PSC	X-ray	2.67	B	1-340	[»]
3PVU	X-ray	2.48	B	1-340	[»]
3PVW	X-ray	2.49	B	1-340	[»]

ProteinModelPortal

P62871.

SMR

P62871. Positions 1-340.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29227N.

IntAct

P62871. 1 interaction.

MINT

MINT-1864875.

STRING

P62871.

Proteomic databases

PRIDE

P62871.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000000259; ENSBTAP00000000259; ENSBTAG00000000215.
ENSBTAT00000045065; ENSBTAP00000042481; ENSBTAG00000000215.

GeneID

281201.

KEGG

bta:281201.

Organism-specific databases

CTD

2782.

Phylogenomic databases

eggNOG

maNOG07373.

GeneTree

ENSGT00550000074331.

HOVERGEN

HBG000188.

InParanoid

P62871.

OMA

CMLWDIE.

OrthoDB

EOG4HX51D.

PhylomeDB

P62871.

Family and domain databases

InterPro

IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

KO

K04536.

Pfam

PF00400. WD40. 7 hits.
[Graphical view]

PIRSF

PIRSF002394. GN-bd_beta. 1 hit.

PRINTS

PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GBB1_BOVIN

Accession

Primary (citable) accession number: P62871
Secondary accession number(s): P04697, P04901, Q3MHF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program