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Protein

Elongin-B

Gene

Elob

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity).By similarity
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • protein complex binding Source: RGD
  • transcription coactivator activity Source: RGD
  • ubiquitin protein ligase binding Source: RGD

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-8951664. Neddylation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongin-B
Short name:
EloB
Alternative name(s):
Elongin 18 kDa subunit
RNA polymerase II transcription factor SIII subunit B
SIII p18
Transcription elongation factor B polypeptide 2
Gene namesi
Name:Elob
Synonyms:Tceb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621200. Elob.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149161 – 118Elongin-BAdd BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei84PhosphothreonineBy similarity1
Modified residuei108PhosphoserineBy similarity1
Modified residuei111PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62870.
PRIDEiP62870.

PTM databases

iPTMnetiP62870.
PhosphoSitePlusiP62870.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004814.
ExpressionAtlasiP62870. baseline and differential.
GenevisibleiP62870. RN.

Interactioni

Subunit structurei

Heterotrimer of an A (ELOA, ELOA2 or ELOA3), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. Part of E3 ubiquitin ligase complexes with CUL5 or CUL2, RBX1 and a substrate adapter protein that can be either SOCS1, SOCS5, ELOA, VHL or WSB1. Interacts with VHL. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Interacts with SPSB1. Interacts with KLHDC10; which may be an E3 ubiquitin ligase complex substrate recognition component.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD
  • ubiquitin protein ligase binding Source: RGD

Protein-protein interaction databases

BioGridi249665. 8 interactors.
CORUMiP62870.
IntActiP62870. 6 interactors.
MINTiMINT-4094305.
STRINGi10116.ENSRNOP00000006892.

Structurei

3D structure databases

ProteinModelPortaliP62870.
SMRiP62870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

eggNOGiKOG4495. Eukaryota.
ENOG4111UGJ. LUCA.
GeneTreeiENSGT00390000018316.
HOGENOMiHOG000293425.
HOVERGENiHBG008581.
InParanoidiP62870.
KOiK03873.
OMAiDCGFTNQ.
PhylomeDBiP62870.

Family and domain databases

InterProiView protein in InterPro
IPR029071. Ubiquitin-like_domsf.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 1 hit.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P62870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPE EQRLYKDDQL
60 70 80 90 100
LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALRI EPFSSPPELP
110
DVMKPQDSGG SANEQAVQ
Length:118
Mass (Da):13,170
Last modified:August 16, 2004 - v1
Checksum:iBA702F24CEC0FC02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42855 mRNA. Translation: AAA80968.1.
BC058463 mRNA. Translation: AAH58463.1.
RefSeqiNP_112391.1. NM_031129.3.
UniGeneiRn.108140.

Genome annotation databases

EnsembliENSRNOT00000087768; ENSRNOP00000072621; ENSRNOG00000004814.
GeneIDi81807.
KEGGirno:81807.
UCSCiRGD:621200. rat.

Similar proteinsi

Entry informationi

Entry nameiELOB_RAT
AccessioniPrimary (citable) accession number: P62870
Secondary accession number(s): Q63529, Q80W20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 22, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways