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Protein

Elongin-B

Gene

Elob

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (PubMed:27863225, PubMed:27863226).By similarity2 Publications
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.
R-MMU-8951664. Neddylation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongin-B
Short name:
EloB
Alternative name(s):
Elongin 18 kDa subunit
RNA polymerase II transcription factor SIII subunit B
SIII p18
Transcription elongation factor B polypeptide 2
Gene namesi
Name:ElobImported
Synonyms:Tceb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1914923. Elob.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149151 – 118Elongin-BAdd BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei84PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei111PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62869.
MaxQBiP62869.
PaxDbiP62869.
PeptideAtlasiP62869.
PRIDEiP62869.

2D gel databases

REPRODUCTION-2DPAGEiP62869.

PTM databases

iPTMnetiP62869.
PhosphoSitePlusiP62869.

Expressioni

Gene expression databases

BgeeiENSMUSG00000055839.
GenevisibleiP62869. MM.

Interactioni

Subunit structurei

Heterotrimer of an A (ELOA, ELOA2 or ELOA3), ELOB and ELOC subunit (PubMed:16498413). The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226). Part of E3 ubiquitin ligase complexes with CUL5 or CUL2, RBX1 and a substrate adapter protein that can be either SOCS1, SOCS5, ELOA, VHL or WSB1. Interacts with VHL. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Interacts with SPSB1. Interacts with KLHDC10; which may be an E3 ubiquitin ligase complex substrate recognition component.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212356. 12 interactors.
IntActiP62869. 8 interactors.
MINTiMINT-2789207.
STRINGi10090.ENSMUSP00000066210.

Structurei

Secondary structure

1118
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Beta strandi12 – 19Combined sources8
Helixi24 – 35Combined sources12
Helixi39 – 41Combined sources3
Beta strandi42 – 46Combined sources5
Helixi57 – 60Combined sources4
Turni64 – 66Combined sources3
Beta strandi73 – 83Combined sources11
Helixi101 – 103Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FNJX-ray1.80B1-118[»]
4JGHX-ray3.00B1-118[»]
ProteinModelPortaliP62869.
SMRiP62869.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62869.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

eggNOGiKOG4495. Eukaryota.
ENOG4111UGJ. LUCA.
GeneTreeiENSGT00390000018316.
HOGENOMiHOG000293425.
HOVERGENiHBG008581.
InParanoidiP62869.
KOiK03873.
OMAiDCGFTNQ.
OrthoDBiEOG091G10A8.
PhylomeDBiP62869.
TreeFamiTF325964.

Family and domain databases

InterProiView protein in InterPro
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 1 hit.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P62869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPE EQRLYKDDQL
60 70 80 90 100
LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALRI EPFSSPPELP
110
DVMKPQDSGG SANEQAVQ
Length:118
Mass (Da):13,170
Last modified:August 16, 2004 - v1
Checksum:iBA702F24CEC0FC02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019358 mRNA. Translation: BAB31677.1.
AK002223 mRNA. Translation: BAB21946.1.
AK002868 mRNA. Translation: BAB22417.1.
AK003277 mRNA. Translation: BAB22684.1.
AK008477 mRNA. Translation: BAB25690.1.
AK008534 mRNA. Translation: BAB25727.1.
AK012254 mRNA. Translation: BAB28121.1.
AK019181 mRNA. Translation: BAB31590.1.
AK019218 mRNA. Translation: BAB31608.1.
BC051927 mRNA. Translation: AAH51927.2.
BC056983 mRNA. Translation: AAH56983.1.
CCDSiCCDS57055.1.
RefSeqiNP_080581.1. NM_026305.2.
UniGeneiMm.153758.

Genome annotation databases

EnsembliENSMUST00000069579; ENSMUSP00000066210; ENSMUSG00000055839.
GeneIDi67673.
KEGGimmu:67673.
UCSCiuc008atr.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiELOB_MOUSE
AccessioniPrimary (citable) accession number: P62869
Secondary accession number(s): Q63529, Q80W20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 7, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references