Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongin-B

Gene

Elob

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (PubMed:27863225, PubMed:27863226).By similarity2 Publications
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Elongin-B
Short name:
EloB
Alternative name(s):
Elongin 18 kDa subunit
RNA polymerase II transcription factor SIII subunit B
SIII p18
Transcription elongation factor B polypeptide 2
Gene namesi
Name:ElobImported
Synonyms:Tceb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1914923 Elob

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149151 – 118Elongin-BAdd BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei84PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei111PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62869
MaxQBiP62869
PaxDbiP62869
PeptideAtlasiP62869
PRIDEiP62869

2D gel databases

REPRODUCTION-2DPAGEiP62869

PTM databases

iPTMnetiP62869
PhosphoSitePlusiP62869

Expressioni

Gene expression databases

BgeeiENSMUSG00000055839
GenevisibleiP62869 MM

Interactioni

Subunit structurei

Heterotrimer of an A (ELOA, ELOA2 or ELOA3), ELOB and ELOC subunit (PubMed:16498413). The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226). Part of E3 ubiquitin ligase complexes with CUL5 or CUL2, RBX1 and a substrate adapter protein that can be either SOCS1, SOCS5, ELOA, VHL or WSB1. Interacts with VHL. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Interacts with SPSB1. Interacts with KLHDC10; which may be an E3 ubiquitin ligase complex substrate recognition component.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212356, 13 interactors
CORUMiP62869
IntActiP62869, 8 interactors
MINTiP62869
STRINGi10090.ENSMUSP00000066210

Structurei

Secondary structure

1118
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Beta strandi12 – 19Combined sources8
Helixi24 – 35Combined sources12
Helixi39 – 41Combined sources3
Beta strandi42 – 46Combined sources5
Helixi57 – 60Combined sources4
Turni64 – 66Combined sources3
Beta strandi73 – 83Combined sources11
Helixi101 – 103Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FNJX-ray1.80B1-118[»]
4JGHX-ray3.00B1-118[»]
ProteinModelPortaliP62869
SMRiP62869
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62869

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

eggNOGiKOG4495 Eukaryota
ENOG4111UGJ LUCA
GeneTreeiENSGT00390000018316
HOGENOMiHOG000293425
HOVERGENiHBG008581
InParanoidiP62869
KOiK03873
OMAiQDCGITV
OrthoDBiEOG091G10A8
PhylomeDBiP62869
TreeFamiTF325964

Family and domain databases

InterProiView protein in InterPro
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P62869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPE EQRLYKDDQL
60 70 80 90 100
LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALRI EPFSSPPELP
110
DVMKPQDSGG SANEQAVQ
Length:118
Mass (Da):13,170
Last modified:August 16, 2004 - v1
Checksum:iBA702F24CEC0FC02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019358 mRNA Translation: BAB31677.1
AK002223 mRNA Translation: BAB21946.1
AK002868 mRNA Translation: BAB22417.1
AK003277 mRNA Translation: BAB22684.1
AK008477 mRNA Translation: BAB25690.1
AK008534 mRNA Translation: BAB25727.1
AK012254 mRNA Translation: BAB28121.1
AK019181 mRNA Translation: BAB31590.1
AK019218 mRNA Translation: BAB31608.1
BC051927 mRNA Translation: AAH51927.2
BC056983 mRNA Translation: AAH56983.1
CCDSiCCDS57055.1
RefSeqiNP_080581.1, NM_026305.2
UniGeneiMm.153758

Genome annotation databases

EnsembliENSMUST00000069579; ENSMUSP00000066210; ENSMUSG00000055839
GeneIDi67673
KEGGimmu:67673
UCSCiuc008atr.1 mouse

Similar proteinsi

Entry informationi

Entry nameiELOB_MOUSE
AccessioniPrimary (citable) accession number: P62869
Secondary accession number(s): Q63529, Q80W20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health