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Protein

40S ribosomal protein S28

Gene

RPS28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

ReactomeiREACT_1079. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1979. Translation initiation complex formation.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_931. Ribosomal scanning and start codon recognition.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S28
Gene namesi
Name:RPS28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10418. RPS28.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • cytosolic small ribosomal subunit Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • small ribosomal subunit Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Diamond-Blackfan anemia 15, with mandibulofacial dysostosis (DBA15)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.

See also OMIM:606164

Keywords - Diseasei

Diamond-Blackfan anemia

Organism-specific databases

MIMi606164. phenotype.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34825.

Polymorphism and mutation databases

BioMutaiRPS28.
DMDMi51338652.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 696940S ribosomal protein S28PRO_0000136822Add
BLAST

Proteomic databases

MaxQBiP62857.
PaxDbiP62857.
PRIDEiP62857.

PTM databases

PhosphoSiteiP62857.

Expressioni

Gene expression databases

BgeeiP62857.
CleanExiHS_RPS28.
ExpressionAtlasiP62857. baseline and differential.
GenevisibleiP62857. HS.

Organism-specific databases

HPAiHPA047132.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CCNDBP1O952733EBI-353027,EBI-748961
KRTAP10-7P604093EBI-353027,EBI-10172290
KRTAP10-8P604103EBI-353027,EBI-10171774
KRTAP2-3P0C7H83EBI-353027,EBI-10196781
NOTCH2NLQ7Z3S93EBI-353027,EBI-945833

Protein-protein interaction databases

BioGridi112148. 108 interactions.
IntActiP62857. 17 interactions.
MINTiMINT-5000390.
STRINGi9606.ENSP00000472469.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Xelectron microscopy5.00Ac1-69[»]
ProteinModelPortaliP62857.
SMRiP62857. Positions 5-68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S28e family.Curated

Phylogenomic databases

eggNOGiCOG2053.
GeneTreeiENSGT00390000003580.
HOVERGENiHBG000219.
InParanoidiP62857.
KOiK02979.
OMAiILCLLET.
OrthoDBiEOG7SN8GQ.
PhylomeDBiP62857.
TreeFamiTF300136.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00292. Ribosomal_S28e.
InterProiIPR012340. NA-bd_OB-fold.
IPR000289. Ribosomal_S28e.
IPR028626. Ribosomal_S28e_CS.
[Graphical view]
PANTHERiPTHR10769. PTHR10769. 1 hit.
PfamiPF01200. Ribosomal_S28e. 1 hit.
[Graphical view]
ProDomiPD005541. Ribosomal_S28e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00961. RIBOSOMAL_S28E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV
60
REGDVLTLLE SEREARRLR
Length:69
Mass (Da):7,841
Last modified:August 16, 2004 - v1
Checksum:i49902FE9376EB74F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661R → P AA sequence (PubMed:8706699).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05091 mRNA. Translation: AAC15855.1.
U58682 mRNA. Translation: AAB07066.1.
AB061846 Genomic DNA. Translation: BAB79484.1.
CR457055 mRNA. Translation: CAG33336.1.
BC000354 mRNA. Translation: AAH00354.1.
BC021239 mRNA. Translation: AAH21239.1.
BC070217 mRNA. Translation: AAH70217.1.
BC070218 mRNA. Translation: AAH70218.1.
AB007164 Genomic DNA. Translation: BAA28594.1.
CCDSiCCDS45953.1.
PIRiS68914.
RefSeqiNP_001022.1. NM_001031.4.
UniGeneiHs.153177.
Hs.322473.

Genome annotation databases

EnsembliENST00000600659; ENSP00000472469; ENSG00000233927.
GeneIDi6234.
KEGGihsa:6234.
UCSCiuc002mjn.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05091 mRNA. Translation: AAC15855.1.
U58682 mRNA. Translation: AAB07066.1.
AB061846 Genomic DNA. Translation: BAB79484.1.
CR457055 mRNA. Translation: CAG33336.1.
BC000354 mRNA. Translation: AAH00354.1.
BC021239 mRNA. Translation: AAH21239.1.
BC070217 mRNA. Translation: AAH70217.1.
BC070218 mRNA. Translation: AAH70218.1.
AB007164 Genomic DNA. Translation: BAA28594.1.
CCDSiCCDS45953.1.
PIRiS68914.
RefSeqiNP_001022.1. NM_001031.4.
UniGeneiHs.153177.
Hs.322473.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Xelectron microscopy5.00Ac1-69[»]
ProteinModelPortaliP62857.
SMRiP62857. Positions 5-68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112148. 108 interactions.
IntActiP62857. 17 interactions.
MINTiMINT-5000390.
STRINGi9606.ENSP00000472469.

PTM databases

PhosphoSiteiP62857.

Polymorphism and mutation databases

BioMutaiRPS28.
DMDMi51338652.

Proteomic databases

MaxQBiP62857.
PaxDbiP62857.
PRIDEiP62857.

Protocols and materials databases

DNASUi6234.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000600659; ENSP00000472469; ENSG00000233927.
GeneIDi6234.
KEGGihsa:6234.
UCSCiuc002mjn.3. human.

Organism-specific databases

CTDi6234.
GeneCardsiGC19P008386.
HGNCiHGNC:10418. RPS28.
HPAiHPA047132.
MIMi603685. gene.
606164. phenotype.
neXtProtiNX_P62857.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34825.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2053.
GeneTreeiENSGT00390000003580.
HOVERGENiHBG000219.
InParanoidiP62857.
KOiK02979.
OMAiILCLLET.
OrthoDBiEOG7SN8GQ.
PhylomeDBiP62857.
TreeFamiTF300136.

Enzyme and pathway databases

ReactomeiREACT_1079. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1979. Translation initiation complex formation.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_931. Ribosomal scanning and start codon recognition.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

GeneWikiiRPS28.
GenomeRNAii6234.
NextBioi24201.
PROiP62857.
SOURCEiSearch...

Gene expression databases

BgeeiP62857.
CleanExiHS_RPS28.
ExpressionAtlasiP62857. baseline and differential.
GenevisibleiP62857. HS.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00292. Ribosomal_S28e.
InterProiIPR012340. NA-bd_OB-fold.
IPR000289. Ribosomal_S28e.
IPR028626. Ribosomal_S28e_CS.
[Graphical view]
PANTHERiPTHR10769. PTHR10769. 1 hit.
PfamiPF01200. Ribosomal_S28e. 1 hit.
[Graphical view]
ProDomiPD005541. Ribosomal_S28e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00961. RIBOSOMAL_S28E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Bhat K.S.
    Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Kim J.M., Bae Y.S.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The human ribosomal protein genes: sequencing and comparative analysis of 73 genes."
    Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S., Maeda N., Minoshima S., Tanaka T., Shimizu N., Kenmochi N.
    Genome Res. 12:379-390(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: B-cell, Muscle and Prostate.
  6. "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry."
    Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K., Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.
    Eur. J. Biochem. 239:144-149(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 48-68.
    Tissue: Placenta.
  7. "A map of 75 human ribosomal protein genes."
    Kenmochi N., Kawaguchi T., Rozen S., Davis E., Goodman N., Hudson T.J., Tanaka T., Page D.C.
    Genome Res. 8:509-523(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 54-69.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Diamond-Blackfan anemia with mandibulofacial dystostosis is heterogeneous, including the novel DBA genes TSR2 and RPS28."
    UW Center for Mendelian Genomics
    Gripp K.W., Curry C., Olney A.H., Sandoval C., Fisher J., Chong J.X., Pilchman L., Sahraoui R., Stabley D.L., Sol-Church K.
    Am. J. Med. Genet. A 164A:2240-2249(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN DBA15.
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  11. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).

Entry informationi

Entry nameiRS28_HUMAN
AccessioniPrimary (citable) accession number: P62857
Secondary accession number(s): P25112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 24, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Ribosomal proteins
    Ribosomal proteins families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.