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Protein

Ubiquitin-conjugating enzyme E2 D2

Gene

Ube2d2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3 (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_276107. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
REACT_310314. IKK complex recruitment mediated by RIP1.
REACT_360804. CLEC7A (Dectin-1) signaling.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 D2 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein D2
Ubiquitin-conjugating enzyme E2(17)KB 2
Ubiquitin-conjugating enzyme E2-17 kDa 2
Ubiquitin-protein ligase D2
Gene namesi
Name:Ube2d2
Synonyms:Ubc4, Ubch4, Ubch5b, Ube2d2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1930715. Ube2d2a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Ubiquitin-conjugating enzyme E2 D2PRO_0000082463Add
BLAST

Proteomic databases

MaxQBiP62838.
PaxDbiP62838.
PRIDEiP62838.

PTM databases

PhosphoSiteiP62838.

Expressioni

Gene expression databases

BgeeiP62838.
CleanExiMM_UBE2D2.
ExpressionAtlasiP62838. baseline and differential.
GenevisibleiP62838. MM.

Interactioni

Subunit structurei

Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Interacts with CNOT4 (via RING domain). Interacts with E3 ubiquitin-protein ligases CBLC, PJA1 and PJA2. Interacts with PDZRN3.2 Publications

Protein-protein interaction databases

BioGridi208052. 3 interactions.
IntActiP62838. 1 interaction.
MINTiMINT-4139124.
STRINGi10090.ENSMUSP00000131206.

Structurei

3D structure databases

ProteinModelPortaliP62838.
SMRiP62838. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiP62838.
KOiK06689.
OMAiYAGGVYF.
OrthoDBiEOG7PCJGX.
PhylomeDBiP62838.
TreeFamiTF101108.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM
Length:147
Mass (Da):16,735
Last modified:August 16, 2004 - v1
Checksum:iC942BE7853CBC355
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62483 mRNA. Translation: AAB05772.1.
BC003923 mRNA. Translation: AAH03923.1.
CCDSiCCDS50254.1.
RefSeqiNP_064296.1. NM_019912.2.
UniGeneiMm.180052.

Genome annotation databases

EnsembliENSMUST00000167406; ENSMUSP00000131206; ENSMUSG00000091896.
ENSMUST00000170693; ENSMUSP00000132446; ENSMUSG00000091896.
GeneIDi56550.
KEGGimmu:56550.
UCSCiuc012bbj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62483 mRNA. Translation: AAB05772.1.
BC003923 mRNA. Translation: AAH03923.1.
CCDSiCCDS50254.1.
RefSeqiNP_064296.1. NM_019912.2.
UniGeneiMm.180052.

3D structure databases

ProteinModelPortaliP62838.
SMRiP62838. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208052. 3 interactions.
IntActiP62838. 1 interaction.
MINTiMINT-4139124.
STRINGi10090.ENSMUSP00000131206.

PTM databases

PhosphoSiteiP62838.

Proteomic databases

MaxQBiP62838.
PaxDbiP62838.
PRIDEiP62838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000167406; ENSMUSP00000131206; ENSMUSG00000091896.
ENSMUST00000170693; ENSMUSP00000132446; ENSMUSG00000091896.
GeneIDi56550.
KEGGimmu:56550.
UCSCiuc012bbj.1. mouse.

Organism-specific databases

CTDi56550.
MGIiMGI:1930715. Ube2d2a.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiP62838.
KOiK06689.
OMAiYAGGVYF.
OrthoDBiEOG7PCJGX.
PhylomeDBiP62838.
TreeFamiTF101108.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_276107. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
REACT_310314. IKK complex recruitment mediated by RIP1.
REACT_360804. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

NextBioi312927.
PROiP62838.
SOURCEiSearch...

Gene expression databases

BgeeiP62838.
CleanExiMM_UBE2D2.
ExpressionAtlasiP62838. baseline and differential.
GenevisibleiP62838. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a murine ubiquitin conjugating enzyme."
    Richardson P., Zon L.I.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary tumor.
  3. "PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain."
    Yu P., Chen Y., Tagle D.A., Cai T.
    Genomics 79:869-874(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PJA1.
    Strain: 129/SvJ.
  4. "Regulation of synaptic growth and maturation by a synapse-associated E3 ubiquitin ligase at the neuromuscular junction."
    Lu Z., Je H.S., Young P., Gross J., Lu B., Feng G.
    J. Cell Biol. 177:1077-1089(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDZRN3.

Entry informationi

Entry nameiUB2D2_MOUSE
AccessioniPrimary (citable) accession number: P62838
Secondary accession number(s): P51669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 24, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.