P62838 (UB2D2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 D2
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein D2
Ubiquitin-conjugating enzyme E2(17)KB 2
Ubiquitin-conjugating enzyme E2-17 kDa 2
Ubiquitin-protein ligase D2

Gene names

Name:	Ube2d2
Synonyms:	Ubc4, Ubch4, Ubch5b, Ube2d2a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3 By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Interacts with CNOT4 (via RING domain). Interacts with E3 ubiquitin-protein ligase PJA2 By similarity. Interacts with E3 ubiquitin-protein ligase PJA1. Interacts with PDZRN3. Ref.3 Ref.4
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K48-linked ubiquitination Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Ubiquitin-conjugating enzyme E2 D2		PRO_0000082463
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62838 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: C942BE7853CBC355	FASTA	147	16,735
	10 20 30 40 50 60 MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY 70 80 90 100 110 120 PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 130 140 PEIARIYKTD REKYNRIARE WTQKYAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a murine ubiquitin conjugating enzyme." Richardson P., Zon L.I. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary tumor.
[3]	"PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain." Yu P., Chen Y., Tagle D.A., Cai T. Genomics 79:869-874(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PJA1. Strain: 129/SvJ.
[4]	"Regulation of synaptic growth and maturation by a synapse-associated E3 ubiquitin ligase at the neuromuscular junction." Lu Z., Je H.S., Young P., Gross J., Lu B., Feng G. J. Cell Biol. 177:1077-1089(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PDZRN3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62483 mRNA. Translation: AAB05772.1. BC003923 mRNA. Translation: AAH03923.1.
IPI	IPI00125135.
RefSeq	NP_064296.1. NM_019912.2.
UniGene	Mm.180052. Mm.490368.
3D structure databases
ProteinModelPortal	P62838.
ModBase	Search...
PTM databases
PhosphoSite	P62838.
Proteomic databases
PaxDb	P62838.
PRIDE	P62838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000167406; ENSMUSP00000131206; ENSMUSG00000091896. ENSMUST00000170693; ENSMUSP00000132446; ENSMUSG00000091896.
GeneID	56550.
KEGG	mmu:56550.
Organism-specific databases
CTD	56550.
MGI	MGI:1930715. Ube2d2a.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
InParanoid	P62838.
KO	K06689.
OMA	KVNFTTR.
OrthoDB	EOG4RR6JK.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P62838.
Bgee	P62838.
CleanEx	MM_UBE2D2.
Genevestigator	P62838.
GermOnline	ENSMUSG00000037096. Mus musculus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	28630.
SOURCE	Search...

Entry information

Entry name

UB2D2_MOUSE

Accession

Primary (citable) accession number: P62838
Secondary accession number(s): P51669

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program