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Protein

Ubiquitin-conjugating enzyme E2 D2

Gene

UBE2D2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.9 Publications

Caution

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation4 Publications
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: ParkinsonsUK-UCL
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05542-MONOMER
BRENDAi2.3.2.B5 2681
2.3.2.B6 2681
2.3.2.B8 2681
ReactomeiR-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-168927 TICAM1, RIP1-mediated IKK complex recruitment
R-HSA-202424 Downstream TCR signaling
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-HSA-8951664 Neddylation
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
R-HSA-937041 IKK complex recruitment mediated by RIP1
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP62837
SIGNORiP62837
UniPathwayiUPA00143

Protein family/group databases

TCDBi3.A.20.1.1 the peroxisomal protein importer (ppi) family

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 D2 (EC:2.3.2.234 Publications)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme D2 (EC:2.3.2.241 Publication)
E2 ubiquitin-conjugating enzyme D2
Ubiquitin carrier protein D2
Ubiquitin-conjugating enzyme E2(17)KB 2
Ubiquitin-conjugating enzyme E2-17 kDa 2
Ubiquitin-protein ligase D2
p53-regulated ubiquitin-conjugating enzyme 1
Gene namesi
Name:UBE2D2
Synonyms:PUBC1, UBC4, UBC5B, UBCH4, UBCH5B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000131508.15
HGNCiHGNC:12475 UBE2D2
MIMi602962 gene
neXtProtiNX_P62837

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63K → A or E: Strongly reduced interaction with CNTO4. 1 Publication1
Mutagenesisi85C → A: Catalytically inactive. Loss of ability to promote FBXW2-mediated GCM1 ubiquitination. Inhibition of TNF-alpha-induced degradation of NFKBIA. 3 Publications1

Organism-specific databases

DisGeNETi7322
OpenTargetsiENSG00000131508
PharmGKBiPA37125

Polymorphism and mutation databases

BioMutaiUBE2D2
DMDMi51338685

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824621 – 147Ubiquitin-conjugating enzyme E2 D2Add BLAST147

Proteomic databases

EPDiP62837
MaxQBiP62837
PaxDbiP62837
PeptideAtlasiP62837
PRIDEiP62837

PTM databases

iPTMnetiP62837
PhosphoSitePlusiP62837

Expressioni

Gene expression databases

BgeeiENSG00000131508
CleanExiHS_UBE2D2
ExpressionAtlasiP62837 baseline and differential
GenevisibleiP62837 HS

Organism-specific databases

HPAiHPA003920

Interactioni

Subunit structurei

Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Interacts with CNOT4 (via RING domain). Interacts with E3 ubiquitin-protein ligases CBLC, PJA1 and PJA2. Interacts with PDZRN3. Interacts with PPP1R11 (By similarity).By similarity4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi113170251 interactors.
DIPiDIP-29267N
IntActiP62837 130 interactors.
MINTiP62837
STRINGi9606.ENSP00000381717

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Beta strandi19 – 28Combined sources10
Beta strandi32 – 38Combined sources7
Beta strandi41 – 43Combined sources3
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 61Combined sources4
Beta strandi66 – 71Combined sources6
Beta strandi76 – 78Combined sources3
Beta strandi82 – 84Combined sources3
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Beta strandi115 – 117Combined sources3
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UR6NMR-A1-147[»]
1W4UNMR-A1-147[»]
2CLWX-ray1.94A/B/C/D1-147[»]
2ESKX-ray1.36A1-147[»]
2ESOX-ray1.50A1-147[»]
2ESPX-ray1.52A1-147[»]
2ESQX-ray1.44A1-147[»]
3A33X-ray2.20A1-147[»]
3JVZX-ray3.30A/B2-147[»]
3JW0X-ray3.10A/B2-147[»]
3L1YX-ray1.60A1-147[»]
3TGDX-ray1.80A1-147[»]
3ZNIX-ray2.21C/G/K/O2-147[»]
4A49X-ray2.21B1-147[»]
4A4BX-ray2.79C1-147[»]
4A4CX-ray2.70C1-147[»]
4AUQX-ray2.18A/D1-147[»]
4DDGX-ray3.30A/B/C/J/K/L1-147[»]
4DDIX-ray3.80D/E/F/G/H/I2-20[»]
4LDTX-ray1.90C1-147[»]
4V3KX-ray2.04A/D2-147[»]
4V3LX-ray1.53A2-147[»]
4WZ3X-ray2.70A1-147[»]
5D0KX-ray2.65A/D/G/J1-147[»]
5D0MX-ray1.91A1-147[»]
5D1KX-ray1.78A1-147[»]
5D1LX-ray1.62A1-147[»]
5D1MX-ray1.58A1-147[»]
5EDVX-ray3.48C/D/I2-147[»]
5MNJX-ray2.16A/E1-147[»]
5ULFX-ray1.80A/C1-147[»]
5ULHX-ray1.95A1-147[»]
5ULKX-ray2.38A1-147[»]
5VZWX-ray2.28A/B1-147[»]
ProteinModelPortaliP62837
SMRiP62837
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62837

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiP62837
KOiK06689
OMAiNDLGRDP
PhylomeDBiP62837
TreeFamiTF101108

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.101 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62837-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM
Length:147
Mass (Da):16,735
Last modified:August 16, 2004 - v1
Checksum:iC942BE7853CBC355
GO
Isoform 2 (identifier: P62837-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: No experimental confirmation available.
Show »
Length:118
Mass (Da):13,635
Checksum:iCC29567199FE8084
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128K → Q in AAC41750 (PubMed:7724550).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0451801 – 29Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39317 mRNA Translation: AAA91460.1
L40146 Genomic DNA Translation: AAC41750.1
AY651263 mRNA Translation: AAX35690.1
AF317220 mRNA Translation: AAK93958.1
AK001311 mRNA Translation: BAG50891.1
AK001428 mRNA Translation: BAG50911.1
AC010378 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62095.1
CH471062 Genomic DNA Translation: EAW62096.1
CH471062 Genomic DNA Translation: EAW62097.1
BC033349 mRNA Translation: AAH33349.1
CCDSiCCDS43369.1 [P62837-1]
CCDS47275.1 [P62837-2]
PIRiI59365
RefSeqiNP_003330.1, NM_003339.2 [P62837-1]
NP_862821.1, NM_181838.1 [P62837-2]
UniGeneiHs.108332

Genome annotation databases

EnsembliENST00000398733; ENSP00000381717; ENSG00000131508 [P62837-1]
ENST00000398734; ENSP00000381718; ENSG00000131508 [P62837-1]
ENST00000505548; ENSP00000424941; ENSG00000131508 [P62837-2]
ENST00000511725; ENSP00000429613; ENSG00000131508 [P62837-2]
GeneIDi7322
KEGGihsa:7322
UCSCiuc003ler.3 human [P62837-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUB2D2_HUMAN
AccessioniPrimary (citable) accession number: P62837
Secondary accession number(s): D3DQC9
, P51669, Q3MN78, Q96RP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 25, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome