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Protein

Ras-related protein Rap-1A

Gene

Rap1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (By similarity). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.By similarity1 Publication

Enzyme regulationi

Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: Ensembl
  • GTP binding Source: RGD
  • protein complex binding Source: UniProtKB
  • protein transporter activity Source: UniProtKB
  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • cellular response to cAMP Source: UniProtKB
  • cellular response to drug Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to nerve growth factor stimulus Source: UniProtKB
  • cellular response to organic cyclic compound Source: RGD
  • establishment of endothelial barrier Source: UniProtKB
  • liver regeneration Source: RGD
  • negative regulation of collagen biosynthetic process Source: RGD
  • negative regulation of synaptic vesicle exocytosis Source: Ensembl
  • nerve growth factor signaling pathway Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of glucose import Source: RGD
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of neuron projection development Source: UniProtKB
  • positive regulation of phagocytosis Source: RGD
  • positive regulation of protein kinase activity Source: UniProtKB
  • positive regulation of vasculogenesis Source: UniProtKB
  • protein transport Source: UniProtKB
  • Rap protein signal transduction Source: UniProtKB
  • regulation of cell junction assembly Source: UniProtKB
  • response to antineoplastic agent Source: RGD
  • response to carbohydrate Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-170984. ARMS-mediated activation.
R-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-392517. Rap1 signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1A
Alternative name(s):
Ras-related protein Krev-1
Gene namesi
Name:Rap1a
Synonyms:Krev1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1359694. Rap1a.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • early endosome Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • guanyl-nucleotide exchange factor complex Source: Ensembl
  • late endosome Source: UniProtKB
  • myelin sheath Source: Ensembl
  • neuron projection Source: Ensembl
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ras-related protein Rap-1APRO_0000030203Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000030204

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP62836.
PRIDEiP62836.

PTM databases

iPTMnetiP62836.
PhosphoSiteiP62836.

Expressioni

Gene expression databases

GenevisibleiP62836. RN.

Interactioni

Subunit structurei

Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1. In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3. Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi254977. 3 interactions.
MINTiMINT-1865125.
STRINGi10116.ENSRNOP00000040409.

Chemistry

BindingDBiP62836.

Structurei

3D structure databases

ProteinModelPortaliP62836.
SMRiP62836. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP62836.
KOiK04353.
OMAiARSFNSA.
OrthoDBiEOG7QVM41.
PhylomeDBiP62836.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL
Length:184
Mass (Da):20,987
Last modified:August 16, 2004 - v1
Checksum:i42C39290C98E0A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083813 mRNA. Translation: AAH83813.1.
PIRiA61216.
RefSeqiNP_001005765.1. NM_001005765.1.
UniGeneiRn.106321.

Genome annotation databases

EnsembliENSRNOT00000047836; ENSRNOP00000040409; ENSRNOG00000032463.
GeneIDi295347.
KEGGirno:295347.
UCSCiRGD:1359694. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083813 mRNA. Translation: AAH83813.1.
PIRiA61216.
RefSeqiNP_001005765.1. NM_001005765.1.
UniGeneiRn.106321.

3D structure databases

ProteinModelPortaliP62836.
SMRiP62836. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254977. 3 interactions.
MINTiMINT-1865125.
STRINGi10116.ENSRNOP00000040409.

Chemistry

BindingDBiP62836.

PTM databases

iPTMnetiP62836.
PhosphoSiteiP62836.

Proteomic databases

PaxDbiP62836.
PRIDEiP62836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000047836; ENSRNOP00000040409; ENSRNOG00000032463.
GeneIDi295347.
KEGGirno:295347.
UCSCiRGD:1359694. rat.

Organism-specific databases

CTDi5906.
RGDi1359694. Rap1a.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP62836.
KOiK04353.
OMAiARSFNSA.
OrthoDBiEOG7QVM41.
PhylomeDBiP62836.
TreeFamiTF313014.

Enzyme and pathway databases

ReactomeiR-RNO-170984. ARMS-mediated activation.
R-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-392517. Rap1 signalling.

Miscellaneous databases

NextBioi639425.
PROiP62836.

Gene expression databases

GenevisibleiP62836. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A ras-related gene with transformation suppressor activity."
    Kitayama H., Sugimoto Y., Matsuzaki T., Ikawa Y., Noda M.
    Cell 56:77-84(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Phospholipase C(epsilon): a novel Ras effector."
    Kelley G.G., Reks S.E., Ondrako J.M., Smrcka A.V.
    EMBO J. 20:743-754(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLCE1.
  4. "Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth."
    Hisata S., Sakisaka T., Baba T., Yamada T., Aoki K., Matsuda M., Takai Y.
    J. Cell Biol. 178:843-860(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRAP1A_RAT
AccessioniPrimary (citable) accession number: P62836
Secondary accession number(s): P10113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.