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Protein

Ras-related protein Rap-1A

Gene

Rap1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (By similarity). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.By similarity1 Publication

Enzyme regulationi

Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: RGD
  • GTP binding Source: RGD
  • protein-containing complex binding Source: UniProtKB
  • protein transporter activity Source: UniProtKB
  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
  • Ras GTPase binding Source: RGD

GO - Biological processi

  • cellular response to cAMP Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to nerve growth factor stimulus Source: UniProtKB
  • cellular response to organic cyclic compound Source: RGD
  • establishment of endothelial barrier Source: UniProtKB
  • liver regeneration Source: RGD
  • negative regulation of collagen biosynthetic process Source: RGD
  • negative regulation of synaptic vesicle exocytosis Source: RGD
  • nerve growth factor signaling pathway Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis Source: RGD
  • positive regulation of glucose import Source: RGD
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of neuron projection development Source: UniProtKB
  • positive regulation of protein kinase activity Source: UniProtKB
  • positive regulation of vasculogenesis Source: UniProtKB
  • protein transport Source: UniProtKB
  • Rap protein signal transduction Source: UniProtKB
  • regulation of cell junction assembly Source: UniProtKB
  • response to antineoplastic agent Source: RGD
  • response to carbohydrate Source: RGD
  • small GTPase mediated signal transduction Source: RGD

Keywordsi

Biological processNeurogenesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-170984 ARMS-mediated activation
R-RNO-354192 Integrin alphaIIb beta3 signaling
R-RNO-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-RNO-372708 p130Cas linkage to MAPK signaling for integrins
R-RNO-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-RNO-392517 Rap1 signalling
R-RNO-5674135 MAP2K and MAPK activation
R-RNO-6798695 Neutrophil degranulation
R-RNO-8875555 MET activates RAP1 and RAC1

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1A
Alternative name(s):
Ras-related protein Krev-1
Gene namesi
Name:Rap1a
Synonyms:Krev1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1359694 Rap1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302031 – 181Ras-related protein Rap-1AAdd BLAST181
PropeptideiPRO_0000030204182 – 184Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181Cysteine methyl esterBy similarity1
Lipidationi181S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP62836
PRIDEiP62836

PTM databases

iPTMnetiP62836
PhosphoSitePlusiP62836

Expressioni

Gene expression databases

BgeeiENSRNOG00000032463
GenevisibleiP62836 RN

Interactioni

Subunit structurei

Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1. In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3. Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain) (By similarity).By similarity

GO - Molecular functioni

  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
  • Ras GTPase binding Source: RGD

Protein-protein interaction databases

BioGridi254977, 3 interactors
IntActiP62836, 1 interactor
STRINGi10116.ENSRNOP00000040409

Structurei

3D structure databases

ProteinModelPortaliP62836
SMRiP62836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP62836
KOiK04353
OMAiQINRQMP
OrthoDBiEOG091G0OOG
PhylomeDBiP62836
TreeFamiTF313014

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL
Length:184
Mass (Da):20,987
Last modified:August 16, 2004 - v1
Checksum:i42C39290C98E0A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083813 mRNA Translation: AAH83813.1
PIRiA61216
RefSeqiNP_001005765.1, NM_001005765.1
UniGeneiRn.106321

Genome annotation databases

EnsembliENSRNOT00000047836; ENSRNOP00000040409; ENSRNOG00000032463
GeneIDi295347
KEGGirno:295347
UCSCiRGD:1359694 rat

Similar proteinsi

Entry informationi

Entry nameiRAP1A_RAT
AccessioniPrimary (citable) accession number: P62836
Secondary accession number(s): P10113
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health