P62835 (RAP1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rap-1A

Alternative name(s):
Ras-related protein Krev-1

Gene names

Name:	Rap1a
Synonyms:	Krev-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner By similarity.
Enzyme regulation	Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR By similarity.
Subunit structure	In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3 By similarity.
Subcellular location	Cell membrane; Lipid-anchor By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Disease	Tumor suppressor
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from direct assay PubMed 12473665. Source: MGI
Cellular_component	guanyl-nucleotide exchange factor complex Inferred from direct assay PubMed 15894621. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 12473665. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Ras-related protein Rap-1A		PRO_0000030201
	Propeptide	182 – 184	3	Removed in mature form By similarity		PRO_0000030202
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	116 – 119	4	GTP By similarity
	Motif	32 – 40	9	Effector region Probable
Amino acid modifications
	Modified residue	181	1	Cysteine methyl ester By similarity
	Lipidation	181	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62835 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 42C39290C98E0A92	FASTA	184	20,987
	10 20 30 40 50 60 MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG 70 80 90 100 110 120 TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL 130 140 150 160 170 180 EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC051419 mRNA. Translation: AAH51419.1. BC083128 mRNA. Translation: AAH83128.1.
IPI	IPI00138406.
RefSeq	NP_663516.1. NM_145541.5.
UniGene	Mm.101922. Mm.365961. Mm.458171.
3D structure databases
ProteinModelPortal	P62835.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62835. 1 interaction.
MINT	MINT-1865111.
STRING	10090.ENSMUSP00000088174.
PTM databases
PhosphoSite	P62835.
Proteomic databases
PaxDb	P62835.
PRIDE	P62835.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000090678; ENSMUSP00000088174; ENSMUSG00000068798.
GeneID	109905.
KEGG	mmu:109905.
UCSC	uc008qve.1. mouse.
Organism-specific databases
CTD	5906.
MGI	MGI:97852. Rap1a.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00700000104472.
HOGENOM	HOG000233973.
HOVERGEN	HBG009351.
InParanoid	P62835.
KO	K04353.
OMA	DSIGTHE.
OrthoDB	EOG45MN6H.
Gene expression databases
Bgee	P62835.
CleanEx	MM_RAP1A.
Genevestigator	P62835.
GermOnline	ENSMUSG00000068798. Mus musculus.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P62835.
NextBio	362985.
SOURCE	Search...

Entry information

Entry name

RAP1A_MOUSE

Accession

Primary (citable) accession number: P62835
Secondary accession number(s): P10113

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program