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Protein

Ras-related protein Rap-1A

Gene

Rap1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (By similarity). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.By similarity1 Publication

Enzyme regulationi

Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • protein complex binding Source: UniProtKB
  • protein transporter activity Source: UniProtKB
  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
  • Ras GTPase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-170984. ARMS-mediated activation.
R-MMU-354192. Integrin alphaIIb beta3 signaling.
R-MMU-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-MMU-372708. p130Cas linkage to MAPK signaling for integrins.
R-MMU-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-MMU-392517. Rap1 signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1A
Alternative name(s):
Ras-related protein Krev-1
Gene namesi
Name:Rap1a
Synonyms:Krev-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:97852. Rap1a.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • early endosome Source: UniProtKB
  • extracellular exosome Source: MGI
  • guanyl-nucleotide exchange factor complex Source: MGI
  • late endosome Source: UniProtKB
  • myelin sheath Source: UniProtKB
  • neuron projection Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ras-related protein Rap-1APRO_0000030201Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000030202

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP62835.
MaxQBiP62835.
PaxDbiP62835.
PRIDEiP62835.
TopDownProteomicsiP62835.

PTM databases

iPTMnetiP62835.
PhosphoSiteiP62835.
SwissPalmiP62835.

Expressioni

Gene expression databases

BgeeiP62835.
CleanExiMM_RAP1A.
ExpressionAtlasiP62835. baseline and differential.
GenevisibleiP62835. MM.

Interactioni

Subunit structurei

Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1. In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3. Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain) (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: UniProtKB
  • Ras GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi225141. 5 interactions.
IntActiP62835. 2 interactions.
MINTiMINT-1865111.
STRINGi10090.ENSMUSP00000088174.

Structurei

3D structure databases

ProteinModelPortaliP62835.
SMRiP62835. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP62835.
KOiK04353.
OMAiARSFNSA.
OrthoDBiEOG7QVM41.
PhylomeDBiP62835.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL
Length:184
Mass (Da):20,987
Last modified:August 16, 2004 - v1
Checksum:i42C39290C98E0A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC051419 mRNA. Translation: AAH51419.1.
BC083128 mRNA. Translation: AAH83128.1.
CCDSiCCDS17711.1.
RefSeqiNP_663516.1. NM_145541.5.
XP_006500970.1. XM_006500907.2.
UniGeneiMm.101922.
Mm.365961.
Mm.458171.

Genome annotation databases

EnsembliENSMUST00000090678; ENSMUSP00000088174; ENSMUSG00000068798.
GeneIDi109905.
KEGGimmu:109905.
UCSCiuc008qve.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC051419 mRNA. Translation: AAH51419.1.
BC083128 mRNA. Translation: AAH83128.1.
CCDSiCCDS17711.1.
RefSeqiNP_663516.1. NM_145541.5.
XP_006500970.1. XM_006500907.2.
UniGeneiMm.101922.
Mm.365961.
Mm.458171.

3D structure databases

ProteinModelPortaliP62835.
SMRiP62835. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225141. 5 interactions.
IntActiP62835. 2 interactions.
MINTiMINT-1865111.
STRINGi10090.ENSMUSP00000088174.

PTM databases

iPTMnetiP62835.
PhosphoSiteiP62835.
SwissPalmiP62835.

Proteomic databases

EPDiP62835.
MaxQBiP62835.
PaxDbiP62835.
PRIDEiP62835.
TopDownProteomicsiP62835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090678; ENSMUSP00000088174; ENSMUSG00000068798.
GeneIDi109905.
KEGGimmu:109905.
UCSCiuc008qve.2. mouse.

Organism-specific databases

CTDi5906.
MGIiMGI:97852. Rap1a.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP62835.
KOiK04353.
OMAiARSFNSA.
OrthoDBiEOG7QVM41.
PhylomeDBiP62835.
TreeFamiTF313014.

Enzyme and pathway databases

ReactomeiR-MMU-170984. ARMS-mediated activation.
R-MMU-354192. Integrin alphaIIb beta3 signaling.
R-MMU-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-MMU-372708. p130Cas linkage to MAPK signaling for integrins.
R-MMU-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-MMU-392517. Rap1 signalling.

Miscellaneous databases

PROiP62835.
SOURCEiSearch...

Gene expression databases

BgeeiP62835.
CleanExiMM_RAP1A.
ExpressionAtlasiP62835. baseline and differential.
GenevisibleiP62835. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  2. "Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1."
    Kanemura H., Satoh T., Bilasy S.E., Ueda S., Hirashima M., Kataoka T.
    Biochem. Biophys. Res. Commun. 387:754-759(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiRAP1A_MOUSE
AccessioniPrimary (citable) accession number: P62835
Secondary accession number(s): P10113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.