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Protein

GTP-binding nuclear protein Ran

Gene

Ran

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 248GTPBy similarity
Nucleotide bindingi65 – 695GTPBy similarity
Nucleotide bindingi122 – 1254GTPBy similarity

GO - Molecular functioni

  • dynein intermediate chain binding Source: RGD
  • GDP binding Source: RGD
  • GTPase activity Source: RGD
  • GTP binding Source: RGD
  • importin-alpha family protein binding Source: RGD
  • poly(A) RNA binding Source: Ensembl
  • pre-miRNA binding Source: Ensembl
  • protein complex binding Source: RGD
  • protein domain specific binding Source: RGD

GO - Biological processi

  • actin cytoskeleton organization Source: Ensembl
  • cell division Source: UniProtKB-KW
  • cellular protein complex localization Source: Ensembl
  • cellular response to mineralocorticoid stimulus Source: RGD
  • hippocampus development Source: RGD
  • microtubule cytoskeleton organization Source: RGD
  • mitotic nuclear division Source: UniProtKB-KW
  • nucleocytoplasmic transport Source: RGD
  • positive regulation of protein binding Source: Ensembl
  • protein export from nucleus Source: RGD
  • protein import into nucleus Source: RGD
  • protein import into nucleus, translocation Source: Ensembl
  • protein localization to nucleolus Source: Ensembl
  • regulation of protein binding Source: RGD
  • response to organic cyclic compound Source: RGD
  • ribosomal large subunit export from nucleus Source: Ensembl
  • ribosomal small subunit export from nucleus Source: Ensembl
  • small GTPase mediated signal transduction Source: InterPro
  • spermatid development Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein Ran
Alternative name(s):
GTPase Ran
Ras-like protein TC4
Ras-related nuclear protein
Gene namesi
Name:Ran
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620367. Ran.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm
  • Melanosome By similarity
  • Nucleus envelope By similarity

  • Note: Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis. Colocalizes with NEMP1 at the nuclear envelope.By similarity

GO - Cellular componenti

  • centriole Source: Ensembl
  • chromatin Source: Ensembl
  • cytoplasm Source: RGD
  • extracellular exosome Source: Ensembl
  • male germ cell nucleus Source: RGD
  • manchette Source: RGD
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: Ensembl
  • midbody Source: Ensembl
  • nuclear pore Source: RGD
  • nucleolus Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
  • protein complex Source: RGD
  • recycling endosome Source: Ensembl
  • RNA nuclear export complex Source: Ensembl
  • sperm flagellum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 216215GTP-binding nuclear protein RanPRO_0000208698Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei60 – 601N6-acetyllysineBy similarity
Modified residuei71 – 711N6-acetyllysine; alternateBy similarity
Cross-linki71 – 71Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei99 – 991N6-acetyllysineBy similarity
Modified residuei159 – 1591N6-acetyllysine; alternateBy similarity
Modified residuei159 – 1591N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP62828.
PRIDEiP62828.

2D gel databases

World-2DPAGE0004:P62828.

PTM databases

iPTMnetiP62828.
PhosphoSiteiP62828.
SwissPalmiP62828.

Expressioni

Gene expression databases

GenevisibleiP62828. RN.

Interactioni

Subunit structurei

Monomer (By similarity). Also forms a complex with CHC1. Found in a complex with nuclear export signal-cargo peptides, RANBP3 and XPO1. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, Ran and XPO1. Interacts with RANBP10. Interacts in its GTP-bound form with BIRC5/survivin at S and M phases of the cell cycle. Interacts with TERT; the interaction requires hydrogen peroxide-mediated phosphorylation of TERT and the nucleus. Interacts with MAD2L2. Interacts with RANBP10 (By similarity). Interacts with RANBP10 (By similarity). Interacts with VRK1, VRK2 and VRK3. Interacts with NEMP1 and KPNB1 (By similarity).By similarity

GO - Molecular functioni

  • dynein intermediate chain binding Source: RGD
  • importin-alpha family protein binding Source: RGD
  • protein complex binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi249998. 3 interactions.
IntActiP62828. 1 interaction.
MINTiMINT-2513897.
STRINGi10116.ENSRNOP00000001247.

Structurei

3D structure databases

ProteinModelPortaliP62828.
SMRiP62828. Positions 2-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

eggNOGiKOG0096. Eukaryota.
ENOG410XNRS. LUCA.
GeneTreeiENSGT00840000129836.
HOVERGENiHBG107376.
InParanoidiP62828.
KOiK07936.
OMAiQYQQEME.
OrthoDBiEOG7C8GJ1.
PhylomeDBiP62828.
TreeFamiTF106302.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51418. RAN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62828-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL
60 70 80 90 100
VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN
110 120 130 140 150
VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS
160 170 180 190 200
AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD
210
LEVAQTTALP DEDDDL
Length:216
Mass (Da):24,423
Last modified:January 23, 2007 - v3
Checksum:iD5C9B7275C34BCE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF306457 mRNA. Translation: AAG33229.1.
BC059123 mRNA. Translation: AAH59123.1.
RefSeqiNP_445891.1. NM_053439.1.
XP_006249347.1. XM_006249285.2.
UniGeneiRn.107698.

Genome annotation databases

EnsembliENSRNOT00000001247; ENSRNOP00000001247; ENSRNOG00000000938.
GeneIDi84509.
KEGGirno:84509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF306457 mRNA. Translation: AAG33229.1.
BC059123 mRNA. Translation: AAH59123.1.
RefSeqiNP_445891.1. NM_053439.1.
XP_006249347.1. XM_006249285.2.
UniGeneiRn.107698.

3D structure databases

ProteinModelPortaliP62828.
SMRiP62828. Positions 2-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249998. 3 interactions.
IntActiP62828. 1 interaction.
MINTiMINT-2513897.
STRINGi10116.ENSRNOP00000001247.

PTM databases

iPTMnetiP62828.
PhosphoSiteiP62828.
SwissPalmiP62828.

2D gel databases

World-2DPAGE0004:P62828.

Proteomic databases

PaxDbiP62828.
PRIDEiP62828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001247; ENSRNOP00000001247; ENSRNOG00000000938.
GeneIDi84509.
KEGGirno:84509.

Organism-specific databases

CTDi5901.
RGDi620367. Ran.

Phylogenomic databases

eggNOGiKOG0096. Eukaryota.
ENOG410XNRS. LUCA.
GeneTreeiENSGT00840000129836.
HOVERGENiHBG107376.
InParanoidiP62828.
KOiK07936.
OMAiQYQQEME.
OrthoDBiEOG7C8GJ1.
PhylomeDBiP62828.
TreeFamiTF106302.

Miscellaneous databases

PROiP62828.

Gene expression databases

GenevisibleiP62828. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51418. RAN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel genes expressed in rat dorsal root ganglion."
    Xiao H., Huang Q., Zhang F., Yang Z., Chen Z., Han Z., Zhang X.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Spinal ganglion.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 39-56 AND 153-166, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiRAN_RAT
AccessioniPrimary (citable) accession number: P62828
Secondary accession number(s): P17080
, P28746, P28747, Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, Q9UEU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.