P62828 (RAN_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding nuclear protein Ran

Alternative name(s):
GTPase Ran
Ras-like protein TC4
Ras-related nuclear protein

Gene names

Name:

Ran

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 By similarity.
Subunit structure	Monomer By similarity. Also forms a complex with CHC1. Found in a complex with nuclear export signal-cargo peptides, RANBP3 and XPO1. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, Ran and XPO1. Interacts with RANBP10. Interacts in its GTP-bound form with BIRC5/survivin at S and M phases of the cell cycle. Interacts with TERT; the interaction requires hydrogen peroxide-mediated phosphorylation of TERT and the nucleus. Interacts with MAD2L2. Interacts with RANBP10 By similarity. Interacts with RANBP10 By similarity. Interacts with VRK1, VRK2 and VRK3 By similarity.
Subcellular location	Nucleus. Cytoplasm. Melanosome By similarity. Note: Predominantly nuclear during interphase By similarity. Becomes dispersed throughout the cytoplasm during mitosis.
Sequence similarities	Belongs to the small GTPase superfamily. Ran family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Protein transport Transport
Cellular component	Cytoplasm Nucleus
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 216	215	GTP-binding nuclear protein Ran		PRO_0000208698
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	65 – 69	5	GTP By similarity
	Nucleotide binding	122 – 125	4	GTP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	60	1	N6-acetyllysine By similarity
	Modified residue	71	1	N6-acetyllysine By similarity
	Modified residue	99	1	N6-acetyllysine By similarity
	Modified residue	147	1	Phosphotyrosine By similarity
	Modified residue	155	1	Phosphotyrosine By similarity
	Modified residue	159	1	N6-acetyllysine By similarity
	Cross-link	71		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62828 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D5C9B7275C34BCE0	FASTA	216	24,423
	10 20 30 40 50 60 MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK 70 80 90 100 110 120 FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC 130 140 150 160 170 180 GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP 190 200 210 ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel genes expressed in rat dorsal root ganglion." Xiao H., Huang Q., Zhang F., Yang Z., Chen Z., Han Z., Zhang X. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Spinal ganglion.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 39-56 AND 153-166, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF306457 mRNA. Translation: AAG33229.1. BC059123 mRNA. Translation: AAH59123.1.
IPI	IPI00212796.
RefSeq	NP_445891.1. NM_053439.1.
UniGene	Rn.107698.
3D structure databases
ProteinModelPortal	P62828.
SMR	P62828. Positions 2-207.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62828. 1 interaction.
STRING	P62828.
PTM databases
PhosphoSite	P62828.
2D gel databases
World-2DPAGE	0004:P62828.
Proteomic databases
PRIDE	P62828.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001247; ENSRNOP00000001247; ENSRNOG00000000938.
GeneID	84509.
KEGG	rno:84509.
UCSC	NM_053439. rat.
Organism-specific databases
CTD	5901.
RGD	620367. Ran.
Phylogenomic databases
eggNOG	roNOG08298.
GeneTree	ENSGT00570000079121.
HOVERGEN	HBG107376.
InParanoid	P62828.
OMA	ERVCENI.
OrthoDB	EOG4640CV.
PhylomeDB	P62828.
Gene expression databases
ArrayExpress	P62828.
Genevestigator	P62828.
GermOnline	ENSRNOG00000000938. Rattus norvegicus.
Family and domain databases
InterPro	IPR002041. Ran_GTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. [Graphical view]
KO	K07936.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00627. GTPRANTC4.
SMART	SM00176. RAN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51418. RAN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617085.

Entry information

Entry name

RAN_RAT

Accession

Primary (citable) accession number: P62828
Secondary accession number(s): P17080 , P28746, P28747, Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, Q9UEU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program