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P62827

- RAN_MOUSE

UniProt

P62827 - RAN_MOUSE

Protein

GTP-binding nuclear protein Ran

Gene

Ran

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 248GTPBy similarity
    Nucleotide bindingi65 – 695GTPBy similarity
    Nucleotide bindingi122 – 1254GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: MGI

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. cellular protein complex localization Source: MGI
    3. cellular protein localization Source: MGI
    4. mitotic nuclear division Source: UniProtKB-KW
    5. protein import into nucleus Source: MGI
    6. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
    REACT_221178. MicroRNA (miRNA) biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding nuclear protein Ran
    Alternative name(s):
    GTPase Ran
    Ras-like protein TC4
    Ras-related nuclear protein
    Gene namesi
    Name:Ran
    Synonyms:Rasl2-8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1333112. Ran.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Melanosome By similarity
    Note: Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. melanosome Source: UniProtKB-SubCell
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi24 – 241T → N: No effect on RANBP10-binding. 1 Publication
    Mutagenesisi69 – 691Q → L: Partial decrease in RANBP10-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 216215GTP-binding nuclear protein RanPRO_0000208697Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei60 – 601N6-acetyllysineBy similarity
    Modified residuei71 – 711N6-acetyllysine; alternateBy similarity
    Cross-linki71 – 71Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Modified residuei99 – 991N6-acetyllysineBy similarity
    Modified residuei159 – 1591N6-acetyllysine; alternate1 Publication
    Modified residuei159 – 1591N6-succinyllysine; alternate1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP62827.
    PaxDbiP62827.
    PRIDEiP62827.

    2D gel databases

    REPRODUCTION-2DPAGEP62827.

    PTM databases

    PhosphoSiteiP62827.

    Expressioni

    Tissue specificityi

    Expressed in a variety of tissues, including testis.1 Publication

    Gene expression databases

    BgeeiP62827.
    CleanExiMM_RAN.
    GenevestigatoriP62827.

    Interactioni

    Subunit structurei

    Monomer By similarity. Also forms a complex with CHC1. Found in a complex with nuclear export signal-cargo peptides, RANBP3 and XPO1. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, Ran and XPO1. Interacts with RANBP10. Interacts in its GTP-bound form with BIRC5/survivin at S and M phases of the cell cycle. Interacts with TERT; the interaction requires hydrogen peroxide-mediated phosphorylation of TERT and transports TERT to the nucleus. Interacts with MAD2L2 By similarity. Interacts with RANBP10. Interacts with VRK1, VRK2 and VRK3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202580. 4 interactions.
    IntActiP62827. 6 interactions.
    MINTiMINT-1869723.

    Structurei

    3D structure databases

    ProteinModelPortaliP62827.
    SMRiP62827. Positions 8-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ran family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000117729.
    HOVERGENiHBG107376.
    InParanoidiP62827.
    KOiK07936.
    OMAiVHPLSFS.
    OrthoDBiEOG7C8GJ1.
    PhylomeDBiP62827.
    TreeFamiTF106302.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR002041. Ran_GTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00627. GTPRANTC4.
    SMARTiSM00176. RAN. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51418. RAN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62827-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL    50
    VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN 100
    VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS 150
    AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD 200
    LEVAQTTALP DEDDDL 216
    Length:216
    Mass (Da):24,423
    Last modified:January 23, 2007 - v3
    Checksum:iD5C9B7275C34BCE0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91V → G in BAB27105. (PubMed:16141072)Curated
    Sequence conflicti43 – 431L → P in BAB27105. (PubMed:16141072)Curated
    Sequence conflicti49 – 491P → T in AAK14838. (PubMed:1555775)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32751 mRNA. Translation: AAA64247.1.
    S83456 mRNA. Translation: AAB50841.1.
    AF159256 mRNA. Translation: AAD45343.1.
    AK010569 mRNA. Translation: BAB27034.1.
    AK010672 mRNA. Translation: BAB27105.1.
    AK012268 mRNA. No translation available.
    AK012844 mRNA. No translation available.
    AK075900 mRNA. Translation: BAC36040.1.
    AK087974 mRNA. Translation: BAC40068.1.
    AK151341 mRNA. Translation: BAE30319.1.
    AK151937 mRNA. Translation: BAE30813.1.
    AK153197 mRNA. Translation: BAE31797.1.
    AK153411 mRNA. Translation: BAE31971.1.
    AK160647 mRNA. Translation: BAE35939.1.
    AK167040 mRNA. Translation: BAE39208.1.
    AK168183 mRNA. Translation: BAE40143.1.
    BC014829 mRNA. Translation: AAH14829.3.
    BC083356 mRNA. Translation: AAH83356.1.
    M79316 mRNA. Translation: AAK14838.1.
    CCDSiCCDS19693.1.
    PIRiI57020.
    JH0654.
    RefSeqiNP_033417.1. NM_009391.3.
    XP_006504343.1. XM_006504280.1.
    XP_006544502.1. XM_006544439.1.
    UniGeneiMm.297440.
    Mm.386831.

    Genome annotation databases

    EnsembliENSMUST00000031383; ENSMUSP00000031383; ENSMUSG00000029430.
    ENSMUST00000111343; ENSMUSP00000106975; ENSMUSG00000029430.
    GeneIDi102641332.
    19384.
    KEGGimmu:102641332.
    mmu:19384.
    UCSCiuc008zsr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32751 mRNA. Translation: AAA64247.1 .
    S83456 mRNA. Translation: AAB50841.1 .
    AF159256 mRNA. Translation: AAD45343.1 .
    AK010569 mRNA. Translation: BAB27034.1 .
    AK010672 mRNA. Translation: BAB27105.1 .
    AK012268 mRNA. No translation available.
    AK012844 mRNA. No translation available.
    AK075900 mRNA. Translation: BAC36040.1 .
    AK087974 mRNA. Translation: BAC40068.1 .
    AK151341 mRNA. Translation: BAE30319.1 .
    AK151937 mRNA. Translation: BAE30813.1 .
    AK153197 mRNA. Translation: BAE31797.1 .
    AK153411 mRNA. Translation: BAE31971.1 .
    AK160647 mRNA. Translation: BAE35939.1 .
    AK167040 mRNA. Translation: BAE39208.1 .
    AK168183 mRNA. Translation: BAE40143.1 .
    BC014829 mRNA. Translation: AAH14829.3 .
    BC083356 mRNA. Translation: AAH83356.1 .
    M79316 mRNA. Translation: AAK14838.1 .
    CCDSi CCDS19693.1.
    PIRi I57020.
    JH0654.
    RefSeqi NP_033417.1. NM_009391.3.
    XP_006504343.1. XM_006504280.1.
    XP_006544502.1. XM_006544439.1.
    UniGenei Mm.297440.
    Mm.386831.

    3D structure databases

    ProteinModelPortali P62827.
    SMRi P62827. Positions 8-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202580. 4 interactions.
    IntActi P62827. 6 interactions.
    MINTi MINT-1869723.

    PTM databases

    PhosphoSitei P62827.

    2D gel databases

    REPRODUCTION-2DPAGE P62827.

    Proteomic databases

    MaxQBi P62827.
    PaxDbi P62827.
    PRIDEi P62827.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031383 ; ENSMUSP00000031383 ; ENSMUSG00000029430 .
    ENSMUST00000111343 ; ENSMUSP00000106975 ; ENSMUSG00000029430 .
    GeneIDi 102641332.
    19384.
    KEGGi mmu:102641332.
    mmu:19384.
    UCSCi uc008zsr.1. mouse.

    Organism-specific databases

    CTDi 5901.
    MGIi MGI:1333112. Ran.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000117729.
    HOVERGENi HBG107376.
    InParanoidi P62827.
    KOi K07936.
    OMAi VHPLSFS.
    OrthoDBi EOG7C8GJ1.
    PhylomeDBi P62827.
    TreeFami TF106302.

    Enzyme and pathway databases

    Reactomei REACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
    REACT_221178. MicroRNA (miRNA) biogenesis.

    Miscellaneous databases

    NextBioi 296489.
    PROi P62827.
    SOURCEi Search...

    Gene expression databases

    Bgeei P62827.
    CleanExi MM_RAN.
    Genevestigatori P62827.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR002041. Ran_GTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00627. GTPRANTC4.
    SMARTi SM00176. RAN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51418. RAN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "Restoration of lipopolysaccharide-mediated B-cell response after expression of a cDNA encoding a GTP-binding protein."
      Kang A.D., Wong P.M., Chen H., Castagna R., Chung S.W., Sultzer B.M.
      Infect. Immun. 64:4612-4617(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H/HeJ.
      Tissue: Spleen.
    3. "Lps(d)/Ran of endotoxin-resistant C3H/HeJ mice is defective in mediating lipopolysaccharide endotoxin responses."
      Wong P.M.C., Kang A., Chen H., Yuan Q., Fan P., Sultzer B.M., Kan Y.W., Chung S.-W.
      Proc. Natl. Acad. Sci. U.S.A. 96:11543-11548(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H/HeJ.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BALB/c, C57BL/6J and NOD.
      Tissue: Bone marrow, Embryo, Embryonic stem cell, Thymus and Tongue.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary tumor.
    6. "The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach."
      Chavrier P., Simons K., Zerial M.
      Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-70.
      Tissue: Kidney.
    7. Bienvenut W.V., Sandilands E., Serrels B., Brunton V.G., Frame M.C.
      Submitted (FEB-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-23; 57-71 AND 143-152, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryonic fibroblast.
    8. "RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules."
      Schulze H., Dose M., Korpal M., Meyer I., Italiano J.E. Jr., Shivdasani R.A.
      J. Biol. Chem. 283:14109-14119(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RANBP10, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-24 AND GLN-69.
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-159, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-159, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiRAN_MOUSE
    AccessioniPrimary (citable) accession number: P62827
    Secondary accession number(s): P17080
    , P28746, P28747, Q3U954, Q811M2, Q86V08, Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, Q9UEU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3