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Protein

GTP-binding nuclear protein Ran

Gene

Ran

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.By similarity
The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24GTPBy similarity8
Nucleotide bindingi65 – 69GTPBy similarity5
Nucleotide bindingi122 – 125GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1655829. Regulation of cholesterol biosynthesis by SREBP (SREBF).
R-MMU-203927. MicroRNA (miRNA) biogenesis.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein Ran
Alternative name(s):
GTPase Ran
Ras-like protein TC4
Ras-related nuclear protein
Gene namesi
Name:Ran
Synonyms:Rasl2-8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1333112. Ran.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • centriole Source: MGI
  • chromatin Source: Ensembl
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • extracellular matrix Source: MGI
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: MGI
  • midbody Source: MGI
  • nuclear envelope Source: UniProtKB
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • recycling endosome Source: MGI
  • RNA nuclear export complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24T → N: No effect on RANBP10-binding. Loss of interaction with NEMP1. 2 Publications1
Mutagenesisi42T → A: Loss of interaction with NEMP1. 1 Publication1
Mutagenesisi69Q → L: Partial decrease in RANBP10-binding. No effect on interaction with NEMP1 and KPNB1. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002086972 – 216GTP-binding nuclear protein RanAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei24PhosphothreonineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei71N6-acetyllysine; alternateBy similarity1
Cross-linki71Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei99N6-acetyllysineBy similarity1
Modified residuei159N6-acetyllysine; alternateCombined sources1
Modified residuei159N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP62827.
MaxQBiP62827.
PaxDbiP62827.
PeptideAtlasiP62827.
PRIDEiP62827.

2D gel databases

REPRODUCTION-2DPAGEP62827.

PTM databases

iPTMnetiP62827.
PhosphoSitePlusiP62827.
SwissPalmiP62827.

Expressioni

Tissue specificityi

Expressed in a variety of tissues, including testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000029430.
CleanExiMM_RAN.
GenevisibleiP62827. MM.

Interactioni

Subunit structurei

Monomer. Also forms a complex with CHC1 and interacts with the AR N-terminal poly-Gln region. The interaction with AR is inversely correlated with the poly-Gln length. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Found in a nuclear export complex with RANBP3 and XPO1. Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, RAN and XPO1. Interacts with RANBP10. Interacts in its GTP-bound form with BIRC5/survivin at S and M phases of the cell cycle. Interacts with TERT; the interaction requires hydrogen peroxide-mediated phosphorylation of TERT and transports TERT to the nucleus. Interacts with MAD2L2. Interacts with RANBP10 (By similarity). Interacts with VRK1, VRK2 and VRK3. Interacts with NEMP1 and KPNB1 (PubMed:25946333). Interacts (GDP-bound form) with NUTF2; regulates RAN nuclear import. Interacts with CAPG; mediates CAPG nuclear import (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202580. 8 interactors.
IntActiP62827. 6 interactors.
MINTiMINT-1869723.
STRINGi10090.ENSMUSP00000031383.

Structurei

3D structure databases

ProteinModelPortaliP62827.
SMRiP62827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

eggNOGiKOG0096. Eukaryota.
ENOG410XNRS. LUCA.
GeneTreeiENSGT00840000129836.
HOVERGENiHBG107376.
InParanoidiP62827.
KOiK07936.
OMAiVEMPALK.
OrthoDBiEOG091G0L3X.
PhylomeDBiP62827.
TreeFamiTF106302.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51418. RAN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL
60 70 80 90 100
VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN
110 120 130 140 150
VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS
160 170 180 190 200
AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD
210
LEVAQTTALP DEDDDL
Length:216
Mass (Da):24,423
Last modified:January 23, 2007 - v3
Checksum:iD5C9B7275C34BCE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9V → G in BAB27105 (PubMed:16141072).Curated1
Sequence conflicti43L → P in BAB27105 (PubMed:16141072).Curated1
Sequence conflicti49P → T in AAK14838 (PubMed:1555775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32751 mRNA. Translation: AAA64247.1.
S83456 mRNA. Translation: AAB50841.1.
AF159256 mRNA. Translation: AAD45343.1.
AK010569 mRNA. Translation: BAB27034.1.
AK010672 mRNA. Translation: BAB27105.1.
AK012268 mRNA. No translation available.
AK012844 mRNA. No translation available.
AK075900 mRNA. Translation: BAC36040.1.
AK087974 mRNA. Translation: BAC40068.1.
AK151341 mRNA. Translation: BAE30319.1.
AK151937 mRNA. Translation: BAE30813.1.
AK153197 mRNA. Translation: BAE31797.1.
AK153411 mRNA. Translation: BAE31971.1.
AK160647 mRNA. Translation: BAE35939.1.
AK167040 mRNA. Translation: BAE39208.1.
AK168183 mRNA. Translation: BAE40143.1.
BC014829 mRNA. Translation: AAH14829.3.
BC083356 mRNA. Translation: AAH83356.1.
M79316 mRNA. Translation: AAK14838.1.
CCDSiCCDS19693.1.
PIRiI57020.
JH0654.
RefSeqiNP_033417.1. NM_009391.3.
XP_006504343.1. XM_006504280.3.
UniGeneiMm.297440.
Mm.386831.

Genome annotation databases

EnsembliENSMUST00000031383; ENSMUSP00000031383; ENSMUSG00000029430.
ENSMUST00000111343; ENSMUSP00000106975; ENSMUSG00000029430.
GeneIDi19384.
KEGGimmu:19384.
UCSCiuc008zsr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32751 mRNA. Translation: AAA64247.1.
S83456 mRNA. Translation: AAB50841.1.
AF159256 mRNA. Translation: AAD45343.1.
AK010569 mRNA. Translation: BAB27034.1.
AK010672 mRNA. Translation: BAB27105.1.
AK012268 mRNA. No translation available.
AK012844 mRNA. No translation available.
AK075900 mRNA. Translation: BAC36040.1.
AK087974 mRNA. Translation: BAC40068.1.
AK151341 mRNA. Translation: BAE30319.1.
AK151937 mRNA. Translation: BAE30813.1.
AK153197 mRNA. Translation: BAE31797.1.
AK153411 mRNA. Translation: BAE31971.1.
AK160647 mRNA. Translation: BAE35939.1.
AK167040 mRNA. Translation: BAE39208.1.
AK168183 mRNA. Translation: BAE40143.1.
BC014829 mRNA. Translation: AAH14829.3.
BC083356 mRNA. Translation: AAH83356.1.
M79316 mRNA. Translation: AAK14838.1.
CCDSiCCDS19693.1.
PIRiI57020.
JH0654.
RefSeqiNP_033417.1. NM_009391.3.
XP_006504343.1. XM_006504280.3.
UniGeneiMm.297440.
Mm.386831.

3D structure databases

ProteinModelPortaliP62827.
SMRiP62827.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202580. 8 interactors.
IntActiP62827. 6 interactors.
MINTiMINT-1869723.
STRINGi10090.ENSMUSP00000031383.

PTM databases

iPTMnetiP62827.
PhosphoSitePlusiP62827.
SwissPalmiP62827.

2D gel databases

REPRODUCTION-2DPAGEP62827.

Proteomic databases

EPDiP62827.
MaxQBiP62827.
PaxDbiP62827.
PeptideAtlasiP62827.
PRIDEiP62827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031383; ENSMUSP00000031383; ENSMUSG00000029430.
ENSMUST00000111343; ENSMUSP00000106975; ENSMUSG00000029430.
GeneIDi19384.
KEGGimmu:19384.
UCSCiuc008zsr.1. mouse.

Organism-specific databases

CTDi5901.
MGIiMGI:1333112. Ran.

Phylogenomic databases

eggNOGiKOG0096. Eukaryota.
ENOG410XNRS. LUCA.
GeneTreeiENSGT00840000129836.
HOVERGENiHBG107376.
InParanoidiP62827.
KOiK07936.
OMAiVEMPALK.
OrthoDBiEOG091G0L3X.
PhylomeDBiP62827.
TreeFamiTF106302.

Enzyme and pathway databases

ReactomeiR-MMU-1655829. Regulation of cholesterol biosynthesis by SREBP (SREBF).
R-MMU-203927. MicroRNA (miRNA) biogenesis.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

PROiP62827.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029430.
CleanExiMM_RAN.
GenevisibleiP62827. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51418. RAN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAN_MOUSE
AccessioniPrimary (citable) accession number: P62827
Secondary accession number(s): P17080
, P28746, P28747, Q3U954, Q811M2, Q86V08, Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, Q9UEU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.