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Protein

Ras-related protein Rab-1A

Gene

RAB1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26GTPCombined sources4 Publications9
Nucleotide bindingi36 – 43GTPCombined sources4 Publications8
Nucleotide bindingi66 – 70GTPCombined sources1 Publication5
Nucleotide bindingi124 – 127GTPCombined sources4 Publications4
Nucleotide bindingi154 – 156GTPCombined sources4 Publications3

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • autophagy Source: UniProtKB
  • cargo loading into COPII-coated vesicle Source: UniProtKB
  • cell migration Source: UniProtKB
  • COPII vesicle coating Source: Reactome
  • defense response to bacterium Source: UniProtKB
  • endocytosis Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi organization Source: UniProtKB
  • growth hormone secretion Source: UniProtKB
  • interleukin-8 secretion Source: UniProtKB
  • melanosome transport Source: Ensembl
  • positive regulation of glycoprotein metabolic process Source: UniProtKB
  • positive regulation of ubiquitin protein ligase activity Source: Reactome
  • post-translational protein modification Source: Reactome
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
  • substrate adhesion-dependent cell spreading Source: Ensembl
  • vesicle-mediated transport Source: ProtInc
  • vesicle transport along microtubule Source: UniProtKB
  • virion assembly Source: UniProtKB

Keywordsi

Biological processAutophagy, ER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-204005 COPII-mediated vesicle transport
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-8873719 RAB geranylgeranylation
R-HSA-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-1A
Alternative name(s):
YPT1-related protein
Gene namesi
Name:RAB1A
Synonyms:RAB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000138069.16
HGNCiHGNC:9758 RAB1A
MIMi179508 gene
neXtProtiNX_P62820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124N → I: Dominant negative mutant. Strongly reduces the levels of CASR present at the cell-surface. 1 Publication1

Organism-specific databases

DisGeNETi5861
OpenTargetsiENSG00000138069
PharmGKBiPA34107

Polymorphism and mutation databases

BioMutaiRAB1A
DMDMi51338603

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001210562 – 205Ras-related protein Rab-1AAdd BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei79O-(2-cholinephosphoryl)serine2 Publications1
Modified residuei194Phosphoserine; by CDK11 Publication1
Lipidationi204S-geranylgeranyl cysteine1 Publication1
Lipidationi205S-geranylgeranyl cysteine1 Publication1

Post-translational modificationi

Phosphorylated by CDK1 kinase during mitosis.3 Publications
Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

EPDiP62820
PaxDbiP62820
PeptideAtlasiP62820
PRIDEiP62820

PTM databases

iPTMnetiP62820
PhosphoSitePlusiP62820
SwissPalmiP62820

Expressioni

Gene expression databases

BgeeiENSG00000138069
CleanExiHS_RAB1A
ExpressionAtlasiP62820 baseline and differential
GenevisibleiP62820 HS

Organism-specific databases

HPAiCAB005331
HPA056141

Interactioni

Subunit structurei

May interact with YIPF5 (By similarity). Interacts with C9orf72; the interaction mediates recruitment of RAB1A to the ATG1/ULK1 kinase complex (PubMed:27334615). Interacts with GDI1; this promotes dissociation from membranes (PubMed:23815289, PubMed:20176951).By similarity3 Publications
(Microbial infection) Interacts with L.pneumophila AnkX.1 Publication
(Microbial infection) Interacts with L.pneumophila Lem3.1 Publication
(Microbial infection) Interacts with L.pneumophila LidA.1 Publication
(Microbial infection) Interacts with L.pneumophila DrrA; this disrupts the interaction between RAB1A and GDI1 and promotes the exchange of RAB1A-bound GDP with GTP.2 Publications
(Microbial infection) Interacts with E.coli EspG and S.flexneri VirA; this impairs ER to Golgi trafficking and protein secretion.1 Publication
(Microbial infection) Interacts with L.pneumophila and L.drancourtii LepB; this enhances RAB1A GTPase activity.2 Publications
(Microbial infection) Identified in a complex composed of RAB1A, ARF6 and E.coli EspG.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111799, 169 interactors
DIPiDIP-1063N
IntActiP62820, 54 interactors
MINTiP62820
STRINGi9606.ENSP00000387286

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 17Combined sources8
Helixi24 – 28Combined sources5
Beta strandi30 – 32Combined sources3
Helixi39 – 44Combined sources6
Beta strandi47 – 55Combined sources9
Beta strandi58 – 65Combined sources8
Helixi70 – 72Combined sources3
Helixi78 – 80Combined sources3
Turni81 – 83Combined sources3
Beta strandi85 – 92Combined sources8
Helixi96 – 112Combined sources17
Beta strandi117 – 125Combined sources9
Helixi129 – 131Combined sources3
Helixi136 – 145Combined sources10
Beta strandi150 – 154Combined sources5
Turni155 – 157Combined sources3
Helixi159 – 175Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FOLX-ray2.63A6-177[»]
2WWXX-ray1.50A4-178[»]
3L0IX-ray2.85B/D1-177[»]
3SFVX-ray1.73A1-176[»]
3TKLX-ray2.18A1-191[»]
4FMBX-ray3.20B/D/F6-176[»]
4FMCX-ray2.80B/D6-176[»]
F14-115[»]
4FMDX-ray3.05B/D6-176[»]
F13-176[»]
4FMEX-ray4.10B/E6-176[»]
4IRUX-ray3.20B/D/F4-177[»]
4JVSX-ray2.78B1-177[»]
ProteinModelPortaliP62820
SMRiP62820
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62820

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 48Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084 Eukaryota
ENOG410XQN5 LUCA
GeneTreeiENSGT00900000140786
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP62820
KOiK07874
OMAiVTDNDTF
OrthoDBiEOG091G0LA6
PhylomeDBiP62820
TreeFamiTF300097

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62820-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI
60 70 80 90 100
RTIELDGKTI KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN
110 120 130 140 150
NVKQWLQEID RYASENVNKL LVGNKCDLTT KKVVDYTTAK EFADSLGIPF
160 170 180 190 200
LETSAKNATN VEQSFMTMAA EIKKRMGPGA TAGGAEKSNV KIQSTPVKQS

GGGCC
Length:205
Mass (Da):22,678
Last modified:January 23, 2007 - v3
Checksum:iB2A8F4E3B0FB17D6
GO
Isoform 2 (identifier: P62820-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-96: Missing.

Note: No experimental confirmation available.
Show »
Length:141
Mass (Da):15,331
Checksum:i620F588CA34B3A77
GO
Isoform 3 (identifier: P62820-3) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-140: Missing.

Show »
Length:129
Mass (Da):13,903
Checksum:i8325012BF5161AFE
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00552533 – 96Missing in isoform 2. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_00552665 – 140Missing in isoform 3. 2 PublicationsAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28209 mRNA Translation: AAA60240.1
AL050268 mRNA Translation: CAB43369.1
BX571747 mRNA Translation: CAE11872.1
AK055927 mRNA Translation: BAB71048.1
AF498929 mRNA Translation: AAM21077.1
CR533479 mRNA Translation: CAG38510.1
CH471053 Genomic DNA Translation: EAW99921.1
BC000905 mRNA Translation: AAH00905.1
CCDSiCCDS46305.1 [P62820-3]
CCDS46306.1
PIRiA34323 TVHUYP
RefSeqiNP_004152.1, NM_004161.4 [P62820-1]
NP_056358.1, NM_015543.1 [P62820-3]
UniGeneiHs.310645

Genome annotation databases

EnsembliENST00000398529; ENSP00000381540; ENSG00000138069 [P62820-3]
ENST00000409784; ENSP00000387286; ENSG00000138069 [P62820-1]
ENST00000409892; ENSP00000386451; ENSG00000138069 [P62820-2]
GeneIDi5861
KEGGihsa:5861
UCSCiuc002sdn.4 human

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRAB1A_HUMAN
AccessioniPrimary (citable) accession number: P62820
Secondary accession number(s): P11476
, Q6FIE7, Q96N61, Q9Y3T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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