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Protein

Ras-related protein Rab-1A

Gene

RAB1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26GTPCombined sources4 Publications9
Nucleotide bindingi36 – 43GTPCombined sources4 Publications8
Nucleotide bindingi66 – 70GTPCombined sources1 Publication5
Nucleotide bindingi124 – 127GTPCombined sources4 Publications4
Nucleotide bindingi154 – 156GTPCombined sources4 Publications3

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • autophagy Source: UniProtKB
  • cargo loading into COPII-coated vesicle Source: UniProtKB
  • cell migration Source: UniProtKB
  • COPII vesicle coating Source: Reactome
  • defense response to bacterium Source: UniProtKB
  • endocytosis Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi organization Source: UniProtKB
  • growth hormone secretion Source: UniProtKB
  • interleukin-8 secretion Source: UniProtKB
  • melanosome transport Source: Ensembl
  • positive regulation of glycoprotein metabolic process Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
  • small GTPase mediated signal transduction Source: InterPro
  • substrate adhesion-dependent cell spreading Source: Ensembl
  • vesicle-mediated transport Source: ProtInc
  • vesicle transport along microtubule Source: UniProtKB
  • virion assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138069-MONOMER.
ReactomeiR-HSA-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-1A
Alternative name(s):
YPT1-related protein
Gene namesi
Name:RAB1A
Synonyms:RAB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9758. RAB1A.

Subcellular locationi

  • Golgi apparatus 1 Publication
  • Endoplasmic reticulum 1 Publication
  • Early endosome 1 Publication
  • Cytoplasmcytosol 1 Publication
  • Membrane 2 Publications
  • Melanosome By similarity

  • Note: Alternates between membrane-associated and cytosolic forms.1 Publication

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytosol Source: Reactome
  • early endosome Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: GO_Central
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: GO_Central
  • melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124N → I: Dominant negative mutant. Strongly reduces the levels of CASR present at the cell-surface. 1 Publication1

Organism-specific databases

DisGeNETi5861.
OpenTargetsiENSG00000138069.
PharmGKBiPA34107.

Polymorphism and mutation databases

BioMutaiRAB1A.
DMDMi51338603.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001210562 – 205Ras-related protein Rab-1AAdd BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei79O-(2-cholinephosphoryl)serine; by Legionella AnkX2 Publications1
Modified residuei194Phosphoserine; by CDK11 Publication1
Lipidationi204S-geranylgeranyl cysteine1 Publication1
Lipidationi205S-geranylgeranyl cysteine1 Publication1

Post-translational modificationi

Phosphorylated by CDK1 kinase during mitosis.3 Publications
Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

EPDiP62820.
PaxDbiP62820.
PeptideAtlasiP62820.
PRIDEiP62820.

PTM databases

iPTMnetiP62820.
PhosphoSitePlusiP62820.
SwissPalmiP62820.

Expressioni

Gene expression databases

BgeeiENSG00000138069.
CleanExiHS_RAB1A.
ExpressionAtlasiP62820. baseline and differential.
GenevisibleiP62820. HS.

Organism-specific databases

HPAiCAB005331.
HPA056141.

Interactioni

Subunit structurei

May interact with YIPF5. Interacts with C9orf72 (By similarity). Interacts with GDI1; this promotes dissociation from membranes. Interacts with L.pneumophila AnkX, LidA and Lem3. Interacts with L.pneumophila and L.drancourtii LepB; this enhances RAB1A GTPase activity. Interacts with L.pneumophila DrrA; this disrupts the interaction between RAB1A and GDI1 and promotes the exchange of RAB1A-bound GDP with GTP. Interacts with E.coli EspG and S.flexneri VirA; this impairs ER to Golgi trafficking and protein secretion. Identified in a complex composed of RAB1A, ARF6 and E.coli EspG.By similarity10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
drrAQ5ZSQ37EBI-716845,EBI-7632432From a different organism.
GDI1P311502EBI-716845,EBI-946999
MTORP423454EBI-716845,EBI-359260
OCRLQ019687EBI-716845,EBI-6148898
RPTORQ8N1224EBI-716845,EBI-1567928

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi111799. 140 interactors.
DIPiDIP-1063N.
IntActiP62820. 49 interactors.
MINTiMINT-1343792.
STRINGi9606.ENSP00000387286.

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 17Combined sources8
Helixi24 – 28Combined sources5
Beta strandi30 – 32Combined sources3
Helixi39 – 44Combined sources6
Beta strandi47 – 55Combined sources9
Beta strandi58 – 65Combined sources8
Helixi70 – 72Combined sources3
Helixi78 – 80Combined sources3
Turni81 – 83Combined sources3
Beta strandi85 – 92Combined sources8
Helixi96 – 112Combined sources17
Beta strandi117 – 125Combined sources9
Helixi129 – 131Combined sources3
Helixi136 – 145Combined sources10
Beta strandi150 – 154Combined sources5
Turni155 – 157Combined sources3
Helixi159 – 175Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FOLX-ray2.63A6-177[»]
2WWXX-ray1.50A4-178[»]
3L0IX-ray2.85B/D1-177[»]
3SFVX-ray1.73A1-176[»]
3TKLX-ray2.18A1-191[»]
4FMBX-ray3.20B/D/F6-176[»]
4FMCX-ray2.80B/D6-176[»]
F14-115[»]
4FMDX-ray3.05B/D6-176[»]
F13-176[»]
4FMEX-ray4.10B/E6-176[»]
4IRUX-ray3.20B/D/F4-177[»]
4JVSX-ray2.78B1-177[»]
ProteinModelPortaliP62820.
SMRiP62820.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62820.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 48Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP62820.
KOiK07874.
OMAiKERMGNT.
OrthoDBiEOG091G0LA6.
PhylomeDBiP62820.
TreeFamiTF300097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62820-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI
60 70 80 90 100
RTIELDGKTI KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN
110 120 130 140 150
NVKQWLQEID RYASENVNKL LVGNKCDLTT KKVVDYTTAK EFADSLGIPF
160 170 180 190 200
LETSAKNATN VEQSFMTMAA EIKKRMGPGA TAGGAEKSNV KIQSTPVKQS

GGGCC
Length:205
Mass (Da):22,678
Last modified:January 23, 2007 - v3
Checksum:iB2A8F4E3B0FB17D6
GO
Isoform 2 (identifier: P62820-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-96: Missing.

Note: No experimental confirmation available.
Show »
Length:141
Mass (Da):15,331
Checksum:i620F588CA34B3A77
GO
Isoform 3 (identifier: P62820-3) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-140: Missing.

Show »
Length:129
Mass (Da):13,903
Checksum:i8325012BF5161AFE
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00552533 – 96Missing in isoform 2. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_00552665 – 140Missing in isoform 3. 2 PublicationsAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28209 mRNA. Translation: AAA60240.1.
AL050268 mRNA. Translation: CAB43369.1.
BX571747 mRNA. Translation: CAE11872.1.
AK055927 mRNA. Translation: BAB71048.1.
AF498929 mRNA. Translation: AAM21077.1.
CR533479 mRNA. Translation: CAG38510.1.
CH471053 Genomic DNA. Translation: EAW99921.1.
BC000905 mRNA. Translation: AAH00905.1.
CCDSiCCDS46305.1. [P62820-3]
CCDS46306.1.
PIRiA34323. TVHUYP.
RefSeqiNP_004152.1. NM_004161.4. [P62820-1]
NP_056358.1. NM_015543.1. [P62820-3]
UniGeneiHs.310645.

Genome annotation databases

EnsembliENST00000398529; ENSP00000381540; ENSG00000138069. [P62820-3]
ENST00000409784; ENSP00000387286; ENSG00000138069. [P62820-1]
ENST00000409892; ENSP00000386451; ENSG00000138069. [P62820-2]
GeneIDi5861.
KEGGihsa:5861.
UCSCiuc002sdn.4. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28209 mRNA. Translation: AAA60240.1.
AL050268 mRNA. Translation: CAB43369.1.
BX571747 mRNA. Translation: CAE11872.1.
AK055927 mRNA. Translation: BAB71048.1.
AF498929 mRNA. Translation: AAM21077.1.
CR533479 mRNA. Translation: CAG38510.1.
CH471053 Genomic DNA. Translation: EAW99921.1.
BC000905 mRNA. Translation: AAH00905.1.
CCDSiCCDS46305.1. [P62820-3]
CCDS46306.1.
PIRiA34323. TVHUYP.
RefSeqiNP_004152.1. NM_004161.4. [P62820-1]
NP_056358.1. NM_015543.1. [P62820-3]
UniGeneiHs.310645.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FOLX-ray2.63A6-177[»]
2WWXX-ray1.50A4-178[»]
3L0IX-ray2.85B/D1-177[»]
3SFVX-ray1.73A1-176[»]
3TKLX-ray2.18A1-191[»]
4FMBX-ray3.20B/D/F6-176[»]
4FMCX-ray2.80B/D6-176[»]
F14-115[»]
4FMDX-ray3.05B/D6-176[»]
F13-176[»]
4FMEX-ray4.10B/E6-176[»]
4IRUX-ray3.20B/D/F4-177[»]
4JVSX-ray2.78B1-177[»]
ProteinModelPortaliP62820.
SMRiP62820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111799. 140 interactors.
DIPiDIP-1063N.
IntActiP62820. 49 interactors.
MINTiMINT-1343792.
STRINGi9606.ENSP00000387286.

PTM databases

iPTMnetiP62820.
PhosphoSitePlusiP62820.
SwissPalmiP62820.

Polymorphism and mutation databases

BioMutaiRAB1A.
DMDMi51338603.

Proteomic databases

EPDiP62820.
PaxDbiP62820.
PeptideAtlasiP62820.
PRIDEiP62820.

Protocols and materials databases

DNASUi5861.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398529; ENSP00000381540; ENSG00000138069. [P62820-3]
ENST00000409784; ENSP00000387286; ENSG00000138069. [P62820-1]
ENST00000409892; ENSP00000386451; ENSG00000138069. [P62820-2]
GeneIDi5861.
KEGGihsa:5861.
UCSCiuc002sdn.4. human.

Organism-specific databases

CTDi5861.
DisGeNETi5861.
GeneCardsiRAB1A.
HGNCiHGNC:9758. RAB1A.
HPAiCAB005331.
HPA056141.
MIMi179508. gene.
neXtProtiNX_P62820.
OpenTargetsiENSG00000138069.
PharmGKBiPA34107.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP62820.
KOiK07874.
OMAiKERMGNT.
OrthoDBiEOG091G0LA6.
PhylomeDBiP62820.
TreeFamiTF300097.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138069-MONOMER.
ReactomeiR-HSA-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiRAB1A. human.
EvolutionaryTraceiP62820.
GeneWikiiRAB1A.
GenomeRNAii5861.
PROiP62820.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138069.
CleanExiHS_RAB1A.
ExpressionAtlasiP62820. baseline and differential.
GenevisibleiP62820. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB1A_HUMAN
AccessioniPrimary (citable) accession number: P62820
Secondary accession number(s): P11476
, Q6FIE7, Q96N61, Q9Y3T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.