P62819 (S10A3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A3

Alternative name(s):
Protein S-100E
S100 calcium-binding protein A3

Gene names

Name:

S100a3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation By similarity.
Subunit structure	Homodimer and homotetramer for the citrullinated form By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	More than half of the arginine residues undergo citrullination by PAD1 and PAD2. Arg-51 is specifically citrullinated by PAD3 and promotes tetramerization By similarity.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	Calcium Metal-binding Zinc
PTM	Citrullination Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 101	100	Protein S100-A3		PRO_0000143974
Regions
	Domain	12 – 47	36	EF-hand 1
	Domain	50 – 85	36	EF-hand 2
	Calcium binding	20 – 33	14	1; low affinity Potential
	Calcium binding	63 – 74	12	2; high affinity Potential
Sites
	Metal binding	83	1	Zinc By similarity
	Metal binding	86	1	Zinc By similarity
	Metal binding	87	1	Zinc By similarity
	Metal binding	93	1	Zinc By similarity
Amino acid modifications
	Modified residue	51	1	Citrulline; by PAD3 By similarity
	Disulfide bond	30 ↔ 68		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62819 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: EE46EABF16646CCA	FASTA	101	11,747
	10 20 30 40 50 60 MTRPLEQAVA AIVCTFQEYA GRCGDKYKIC QSELKELLQK ELPTWTPSEF RECDYNKFMS 70 80 90 100 VLDTNKDCEV DFGEYVRSLA SLCLYCHEYF KECPPEPPCP Q

References

[1]	"Expression of S100 genes in hair follicle epithelium." Ito M., Kizawa K. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Hair follicle.
[2]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 58-66, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF140231 mRNA. Translation: AAK28305.1.
IPI	IPI00193465.
RefSeq	NP_446133.1. NM_053681.1.
UniGene	Rn.29233.
3D structure databases
ProteinModelPortal	P62819.
SMR	P62819. Positions 3-94.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016034; ENSRNOP00000016034; ENSRNOG00000012008.
GeneID	114216.
KEGG	rno:114216.
UCSC	RGD:620260. rat.
Organism-specific databases
CTD	6274.
RGD	620260. S100a3.
Phylogenomic databases
eggNOG	NOG40471.
GeneTree	ENSGT00690000102087.
HOGENOM	HOG000246968.
HOVERGEN	HBG001479.
InParanoid	P62819.
OMA	YFKDCPP.
OrthoDB	EOG43210N.
Gene expression databases
Genevestigator	P62819.
GermOnline	ENSRNOG00000012008. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR001751. S100/CaBP-9k_CS. IPR013787. S100_Ca-bd_sub. [Graphical view]
Pfam	PF01023. S_100. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. False negative. PS00303. S100_CABP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	297763.

Entry information

Entry name

S10A3_RAT

Accession

Primary (citable) accession number: P62819
Secondary accession number(s): P56566

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program