Gamma-aminobutyric acid receptor subunit alpha-1 precursor

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P62813 (GBRA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-1

Alternative name(s):
GABA(A) receptor subunit alpha-1

Gene names

Name:	Gabra1
Synonyms:	Gabra-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure	Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1. Interacts with TRAK1 By similarity. Ref.8
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chloride transport Traceable author statement PubMed 1346133. Source: RGD gamma-aminobutyric acid signaling pathway Traceable author statement PubMed 1346133. Source: RGD synaptic transmission, GABAergic Inferred from direct assay PubMed 12930820. Source: BHF-UCL
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Inferred from direct assay PubMed 15152020. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GABA-A receptor activity Traceable author statement PubMed 11161482. Source: RGD benzodiazepine receptor activity Traceable author statement PubMed 1346133. Source: RGD chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW drug binding Inferred from direct assay PubMed 1346133. Source: RGD inhibitory extracellular ligand-gated ion channel activity Traceable author statement PubMed 1346133. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Potential

Chain

28 – 455

428

Gamma-aminobutyric acid receptor subunit alpha-1

PRO_0000000430

Regions

Topological domain

28 – 250

223

Extracellular Probable

Transmembrane

251 – 272

Helical; Probable

Transmembrane

278 – 299

Helical; Probable

Transmembrane

312 – 333

Helical; Probable

Topological domain

334 – 420

Cytoplasmic Probable

Transmembrane

421 – 442

Helical; Probable

Amino acid modifications

Modified residue

367

Phosphotyrosine By similarity

Modified residue

374

Phosphotyrosine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

137

N-linked (GlcNAc...) Potential

Disulfide bond

165 ↔ 179

By similarity

Experimental info

Mutagenesis

F → L: Strongly decreases the affinity for GABA-dependent channel gating. Ref.7

Sequence conflict

F → L no nucleotide entry Ref.3

Sequence conflict

435

L → I no nucleotide entry Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P62813 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: A270B43423B4086E
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FASTA

455

51,754

        10         20         30         40         50         60 
MKKSRGLSDY LWAWTLILST LSGRSYGQPS QDELKDNTTV FTRILDRLLD GYDNRLRPGL 

        70         80         90        100        110        120 
GERVTEVKTD IFVTSFGPVS DHDMEYTIDV FFRQSWKDER LKFKGPMTVL RLNNLMASKI 

       130        140        150        160        170        180 
WTPDTFFHNG KKSVAHNMTM PNKLLRITED GTLLYTMRLT VRAECPMHLE DFPMDAHACP 

       190        200        210        220        230        240 
LKFGSYAYTR AEVVYEWTRE PARSVVVAED GSRLNQYDLL GQTVDSGIVQ SSTGEYVVMT 

       250        260        270        280        290        300 
THFHLKRKIG YFVIQTYLPC IMTVILSQVS FWLNRESVPA RTVFGVTTVL TMTTLSISAR 

       310        320        330        340        350        360 
NSLPKVAYAT AMDWFIAVCY AFVFSALIEF ATVNYFTKRG YAWDGKSVVP EKPKKVKDPL 

       370        380        390        400        410        420 
IKKNNTYAPT ATSYTPNLAR GDPGLATIAK SATIEPKEVK PETKPPEPKK TFNSVSKIDR 

       430        440        450 
LSRIAFPLLF GIFNLVYWAT YLNREPQLKA PTPHQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a novel rat GABAA receptor." Lolait S.J., O'Carroll A.-M., Kusano K., Muller J.-M., Brownstein M.J., Mahan L.C. FEBS Lett. 246:145-148(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A novel alpha subunit in rat brain GABAA receptors." Khrestchatisky M., Maclennan A.J., Chiang M.Y., Xu W., Jackson M.B., Brecha N., Sternini C., Olsen R.W., Tobin A.J. Neuron 3:745-753(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands." Malherbe P., Draguhn A., Multhaup G., Beyreuther K., Mohler H. Brain Res. Mol. Brain Res. 8:199-208(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The GABAA receptor family: molecular and functional diversity." Seeburg P.H., Wisden W., Verdoorn T., Pritchett D., Werner P., Herb A., Lueddens H., Sprengel R., Sakmann B. Cold Spring Harb. Symp. Quant. Biol. 55:29-40(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain.
[5]	Groot-Kormelink P.J. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"Point mutations affecting antagonist affinity and agonist dependent gating of GABAA receptor channels." Sigel E., Baur R., Kellenberger S., Malherbe P. EMBO J. 11:2017-2023(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF PHE-91.
[8]	"GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1." Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J. Nat. Neurosci. 4:908-916(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBQLN1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08490 Genomic DNA. Translation: AAC42029.1. AY574250 mRNA. Translation: AAS90346.1. BC100061 mRNA. Translation: AAI00062.1.
IPI	IPI00192642.
PIR	JQ0158.
RefSeq	NP_899155.1. NM_183326.2.
UniGene	Rn.28463.
3D structure databases
ProteinModelPortal	P62813.
SMR	P62813. Positions 42-340.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62813. 1 interaction.
MINT	MINT-3371441.
STRING	10116.ENSRNOP00000004725.
PTM databases
PhosphoSite	P62813.
Proteomic databases
PRIDE	P62813.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004725; ENSRNOP00000004725; ENSRNOG00000003512.
GeneID	29705.
KEGG	rno:29705.
UCSC	RGD:61855. rat.
Organism-specific databases
CTD	2554.
RGD	61855. Gabra1.
Phylogenomic databases
eggNOG	NOG238757.
GeneTree	ENSGT00550000074441.
HOGENOM	HOG000231337.
HOVERGEN	HBG051707.
InParanoid	P62813.
KO	K05175.
OMA	APNIARD.
OrthoDB	EOG4ZS935.
Gene expression databases
Genevestigator	P62813.
GermOnline	ENSRNOG00000003512. Rattus norvegicus.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006028. GABAA_rcpt. IPR001390. GABAAa_rcpt. IPR005431. GABBAa1_rcpt. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 2 hits. [Graphical view]
PRINTS	PR01079. GABAARALPHA. PR01614. GABAARALPHA1. PR00253. GABAARECEPTR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P62813.
ChEMBL	CHEMBL343.
NextBio	285923.

Entry information

Entry name

GBRA1_RAT

Accession

Primary (citable) accession number: P62813
Secondary accession number(s): P18504, Q53YK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents