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Protein

Gamma-aminobutyric acid receptor subunit alpha-1

Gene

Gabra1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225AgonistBy similarity1

GO - Molecular functioni

  • benzodiazepine receptor activity Source: RGD
  • drug binding Source: RGD
  • GABA-A receptor activity Source: RGD
  • GABA-gated chloride ion channel activity Source: Ensembl
  • inhibitory extracellular ligand-gated ion channel activity Source: RGD

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chemical synaptic transmission Source: Reactome
  • chloride transport Source: RGD
  • gamma-aminobutyric acid signaling pathway Source: RGD
  • synaptic transmission, GABAergic Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-112314. Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell.
R-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-1
Alternative name(s):
GABA(A) receptor subunit alpha-1
Gene namesi
Name:Gabra1
Synonyms:Gabra-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61855. Gabra1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 250ExtracellularCuratedAdd BLAST223
Transmembranei251 – 272HelicalCuratedAdd BLAST22
Transmembranei278 – 299HelicalCuratedAdd BLAST22
Transmembranei312 – 333HelicalCuratedAdd BLAST22
Topological domaini334 – 420CytoplasmicCuratedAdd BLAST87
Transmembranei421 – 442HelicalCuratedAdd BLAST22

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • GABA-A receptor complex Source: GO_Central
  • GABA receptor complex Source: BHF-UCL
  • integral component of plasma membrane Source: RGD
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91F → L: Strongly decreases the affinity for GABA-dependent channel gating. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL343.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000000043028 – 455Gamma-aminobutyric acid receptor subunit alpha-1Add BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...)Sequence analysis1
Glycosylationi137N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi165 ↔ 179By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP62813.
PRIDEiP62813.

PTM databases

iPTMnetiP62813.
PhosphoSitePlusiP62813.

Expressioni

Tissue specificityi

Expressed in brain (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000003512.
GenevisibleiP62813. RN.

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains (PubMed:18281286, PubMed:2540033). Interacts with UBQLN1 (PubMed:11528422). Interacts with TRAK1 (By similarity). Interacts with KIF21B (PubMed:25172774).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pik3r1Q637874EBI-6258192,EBI-518443

Protein-protein interaction databases

BioGridi248322. 1 interactor.
IntActiP62813. 2 interactors.
MINTiMINT-3371441.
STRINGi10116.ENSRNOP00000004725.

Chemistry databases

BindingDBiP62813.

Structurei

3D structure databases

ProteinModelPortaliP62813.
SMRiP62813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 122Agonist bindingBy similarity3
Regioni180 – 182Agonist bindingBy similarity3
Regioni290 – 311Allosteric effector bindingBy similarityAdd BLAST22

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP62813.
KOiK05175.
OMAiINTIHFL.
OrthoDBiEOG091G0805.
PhylomeDBiP62813.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005431. GABBAa1_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01614. GABAARALPHA1.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62813-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSRGLSDY LWAWTLILST LSGRSYGQPS QDELKDNTTV FTRILDRLLD
60 70 80 90 100
GYDNRLRPGL GERVTEVKTD IFVTSFGPVS DHDMEYTIDV FFRQSWKDER
110 120 130 140 150
LKFKGPMTVL RLNNLMASKI WTPDTFFHNG KKSVAHNMTM PNKLLRITED
160 170 180 190 200
GTLLYTMRLT VRAECPMHLE DFPMDAHACP LKFGSYAYTR AEVVYEWTRE
210 220 230 240 250
PARSVVVAED GSRLNQYDLL GQTVDSGIVQ SSTGEYVVMT THFHLKRKIG
260 270 280 290 300
YFVIQTYLPC IMTVILSQVS FWLNRESVPA RTVFGVTTVL TMTTLSISAR
310 320 330 340 350
NSLPKVAYAT AMDWFIAVCY AFVFSALIEF ATVNYFTKRG YAWDGKSVVP
360 370 380 390 400
EKPKKVKDPL IKKNNTYAPT ATSYTPNLAR GDPGLATIAK SATIEPKEVK
410 420 430 440 450
PETKPPEPKK TFNSVSKIDR LSRIAFPLLF GIFNLVYWAT YLNREPQLKA

PTPHQ
Length:455
Mass (Da):51,754
Last modified:August 16, 2004 - v1
Checksum:iA270B43423B4086E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91F → L no nucleotide entry (PubMed:1977069).Curated1
Sequence conflicti435L → I no nucleotide entry (PubMed:2540033).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08490 Genomic DNA. Translation: AAC42029.1.
AY574250 mRNA. Translation: AAS90346.1.
BC100061 mRNA. Translation: AAI00062.1.
PIRiJQ0158.
RefSeqiNP_899155.1. NM_183326.2.
XP_006246185.1. XM_006246123.3.
XP_006246186.1. XM_006246124.3.
XP_017452668.1. XM_017597179.1.
UniGeneiRn.28463.

Genome annotation databases

EnsembliENSRNOT00000004725; ENSRNOP00000004725; ENSRNOG00000003512.
GeneIDi29705.
KEGGirno:29705.
UCSCiRGD:61855. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08490 Genomic DNA. Translation: AAC42029.1.
AY574250 mRNA. Translation: AAS90346.1.
BC100061 mRNA. Translation: AAI00062.1.
PIRiJQ0158.
RefSeqiNP_899155.1. NM_183326.2.
XP_006246185.1. XM_006246123.3.
XP_006246186.1. XM_006246124.3.
XP_017452668.1. XM_017597179.1.
UniGeneiRn.28463.

3D structure databases

ProteinModelPortaliP62813.
SMRiP62813.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248322. 1 interactor.
IntActiP62813. 2 interactors.
MINTiMINT-3371441.
STRINGi10116.ENSRNOP00000004725.

Chemistry databases

BindingDBiP62813.
ChEMBLiCHEMBL343.

PTM databases

iPTMnetiP62813.
PhosphoSitePlusiP62813.

Proteomic databases

PaxDbiP62813.
PRIDEiP62813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004725; ENSRNOP00000004725; ENSRNOG00000003512.
GeneIDi29705.
KEGGirno:29705.
UCSCiRGD:61855. rat.

Organism-specific databases

CTDi2554.
RGDi61855. Gabra1.

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP62813.
KOiK05175.
OMAiINTIHFL.
OrthoDBiEOG091G0805.
PhylomeDBiP62813.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-RNO-112314. Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell.
R-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Miscellaneous databases

PROiP62813.

Gene expression databases

BgeeiENSRNOG00000003512.
GenevisibleiP62813. RN.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005431. GABBAa1_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01614. GABAARALPHA1.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBRA1_RAT
AccessioniPrimary (citable) accession number: P62813
Secondary accession number(s): P18504, Q53YK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.