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P62801 (H4_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone H4
Gene names
Name:H4-I
AND
Name:H4-II
AND
Name:H4-III
AND
Name:H4-IV
AND
Name:H4-V
AND
Name:H4-VI
AND
Name:H4-VII
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length103 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit structure

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location

Nucleus. Chromosome.

Post-translational modification

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin By similarity.

Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation By similarity.

Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage By similarity.

Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing By similarity.

Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4 By similarity.

Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me) By similarity.

Sumoylated, which is associated with transcriptional repression By similarity.

Sequence similarities

Belongs to the histone H4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 103102Histone H4
PRO_0000158296

Regions

DNA binding17 – 215

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity
Modified residue41Asymmetric dimethylarginine; by PRMT1; alternate By similarity
Modified residue41Citrulline; alternate By similarity
Modified residue41Omega-N-methylarginine; by PRMT1; alternate By similarity
Modified residue41Symmetric dimethylarginine; by PRMT5 and PRMT7; alternate By similarity
Modified residue61N6-acetyllysine By similarity
Modified residue91N6-acetyllysine By similarity
Modified residue131N6-acetyllysine By similarity
Modified residue171N6-acetyllysine By similarity
Modified residue211N6,N6,N6-trimethyllysine; alternate By similarity
Modified residue211N6,N6-dimethyllysine; alternate By similarity
Modified residue211N6-methylated lysine By similarity
Modified residue211N6-methyllysine; alternate By similarity
Modified residue321N6-acetyllysine By similarity
Modified residue481Phosphoserine; by PAK2 By similarity
Modified residue521Phosphotyrosine By similarity
Modified residue601N6-acetyllysine By similarity
Modified residue801N6-acetyllysine By similarity
Modified residue891Phosphotyrosine By similarity
Modified residue921N6-acetyllysine; alternate By similarity
Cross-link92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate By similarity

Secondary structure

............. 103
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P62801 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: A9E5DFD3F8B97598

FASTA10311,367
        10         20         30         40         50         60 
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK 

        70         80         90        100 
VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG 

« Hide

References

[1]"Inverted duplication of histone genes in chicken and disposition of regulatory sequences."
Wang S.W., Robins A.J., D'Andrea R., Wells J.R.E.
Nucleic Acids Res. 13:1369-1387(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genomic organization, DNA sequence, and expression of chicken embryonic histone genes."
Sugarman B.J., Dodgson J.B., Engel J.D.
J. Biol. Chem. 258:9005-9016(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Nucleotide sequences of two members of the chicken H4 histone-encoding gene family."
Nakayama T., Takechi S., Ohshige T., Kondo K., Yamamoto K.
Gene 108:311-312(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (H4-III AND H4-IV).
[4]"Organization of the chicken histone genes in a major gene cluster and generation of an almost complete set of the core histone protein sequences."
Takami Y., Higashio M., Fukuoka T., Takechi S., Nakayama T.
DNA Res. 3:95-99(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (H4-VI AND H4-VII).
Strain: White leghorn.
Tissue: Liver.
[5]"The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix."
Arents G., Burlingame R.W., Wang B.-C., Love W.E., Moudrianakis E.N.
Proc. Natl. Acad. Sci. U.S.A. 88:10148-10152(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
[6]"Asymmetries in the nucleosome core particle at 2.5 A resolution."
Harp J.M., Hanson B.L., Timm D.E., Bunick G.J.
Acta Crystallogr. D 56:1513-1534(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02218 Genomic DNA. Translation: CAA26137.1.
X02218 Genomic DNA. Translation: CAA26140.1.
J00866 Genomic DNA. No translation available.
M74533 Genomic DNA. Translation: AAA73091.1.
M74534 Genomic DNA. Translation: AAA73092.1.
U37575 Genomic DNA. Translation: AAC59999.1.
U37575 Genomic DNA. Translation: AAC60001.1.
PIRHSCH4. A02640.
JH0507.
RefSeqNP_001032932.1. NM_001037843.1.
NP_001032934.1. NM_001037845.1.
NP_001268414.1. NM_001281485.1.
XP_001233095.1. XM_001233094.3.
XP_001233180.1. XM_001233179.3.
XP_003640418.1. XM_003640370.2.
XP_004937726.1. XM_004937669.1.
XP_425458.2. XM_425458.4.
UniGeneGga.39882.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EQZX-ray2.50D/H1-103[»]
1HIOX-ray3.10D28-103[»]
1HQ3X-ray2.15D/H1-102[»]
1TZYX-ray1.90D/H1-103[»]
2AROX-ray2.10D/H1-103[»]
2HIOX-ray3.10D1-103[»]
3C9Kelectron microscopy20.00D/H2-103[»]
ProteinModelPortalP62801.
SMRP62801. Positions 21-102.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid679227. 5 interactions.
IntActP62801. 2 interactions.
STRING9031.ENSGALP00000037292.

Proteomic databases

PaxDbP62801.
PRIDEP62801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000046233; ENSGALP00000041526; ENSGALG00000025786.
GeneID100858049.
100858319.
417946.
417950.
427884.
770005.
770079.
770142.
KEGGgga:100858049.
gga:100858319.
gga:417946.
gga:417950.
gga:427884.
gga:770005.
gga:770079.
gga:770142.

Organism-specific databases

CTD100858049.
100858319.
417946.
417950.
427884.
770005.
770079.
770142.

Phylogenomic databases

eggNOGCOG2036.
GeneTreeENSGT00750000117311.
HOVERGENHBG051878.
InParanoidP62801.
KOK11254.
PhylomeDBP62801.

Enzyme and pathway databases

ReactomeREACT_197818. Chromatin organization.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR001951. Histone_H4.
IPR019809. Histone_H4_CS.
[Graphical view]
PfamPF00125. Histone. 1 hit.
[Graphical view]
PRINTSPR00623. HISTONEH4.
SMARTSM00417. H4. 1 hit.
[Graphical view]
SUPFAMSSF47113. SSF47113. 1 hit.
PROSITEPS00047. HISTONE_H4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP62801.
NextBio20821175.
PROP62801.

Entry information

Entry nameH4_CHICK
AccessionPrimary (citable) accession number: P62801
Secondary accession number(s): P02304, P02305
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references