Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P62801

- H4_CHICK

UniProt

P62801 - H4_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Histone H4

Gene

H4-I

more
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi17 – 215

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. DNA replication-dependent nucleosome assembly Source: Ensembl
  2. DNA replication-independent nucleosome assembly Source: Ensembl
  3. negative regulation of megakaryocyte differentiation Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_189773. RNA Polymerase I Chain Elongation.
REACT_194991. Oxidative Stress Induced Senescence.
REACT_197049. Amyloids.
REACT_197522. Meiotic recombination.
REACT_197579. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_197820. HATs acetylate histones.
REACT_198068. Condensation of Prophase Chromosomes.
REACT_204005. PRC2 methylates histones and DNA.
REACT_214700. Senescence-Associated Secretory Phenotype (SASP).
REACT_223091. SIRT1 negatively regulates rRNA Expression.
REACT_223355. NoRC negatively regulates rRNA expression.
REACT_224340. RNA Polymerase I Promoter Opening.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H4
Gene namesi
Name:H4-I
AND
Name:H4-II
AND
Name:H4-III
AND
Name:H4-IV
AND
Name:H4-V
AND
Name:H4-VI
AND
Name:H4-VII
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. nuclear chromosome Source: Ensembl
  3. nucleoplasm Source: Reactome
  4. nucleosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 103102Histone H4PRO_0000158296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei4 – 41Asymmetric dimethylarginine; by PRMT1; alternateBy similarity
Modified residuei4 – 41Citrulline; alternateBy similarity
Modified residuei4 – 41Omega-N-methylarginine; by PRMT1; alternateBy similarity
Modified residuei4 – 41Symmetric dimethylarginine; by PRMT5 and PRMT7; alternateBy similarity
Modified residuei6 – 61N6-acetyllysineBy similarity
Modified residuei9 – 91N6-acetyllysineBy similarity
Modified residuei13 – 131N6-acetyllysineBy similarity
Modified residuei17 – 171N6-acetyllysineBy similarity
Modified residuei21 – 211N6,N6,N6-trimethyllysine; alternateBy similarity
Modified residuei21 – 211N6,N6-dimethyllysine; alternateBy similarity
Modified residuei21 – 211N6-methylated lysineBy similarity
Modified residuei21 – 211N6-methyllysine; alternateBy similarity
Modified residuei32 – 321N6-acetyllysineBy similarity
Modified residuei48 – 481Phosphoserine; by PAK2By similarity
Modified residuei52 – 521PhosphotyrosineBy similarity
Modified residuei60 – 601N6-acetyllysineBy similarity
Modified residuei80 – 801N6-acetyllysineBy similarity
Modified residuei89 – 891PhosphotyrosineBy similarity
Modified residuei92 – 921N6-acetyllysine; alternateBy similarity
Cross-linki92 – 92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationi

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.By similarity
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.By similarity
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity).By similarity
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing (By similarity).By similarity
Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4 (By similarity).By similarity
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me) (By similarity).By similarity
Sumoylated, which is associated with transcriptional repression.By similarity

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62801.
PRIDEiP62801.

Expressioni

Gene expression databases

ExpressionAtlasiP62801. baseline and differential.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Protein-protein interaction databases

BioGridi679227. 6 interactions.
IntActiP62801. 2 interactions.
STRINGi9031.ENSGALP00000037292.

Structurei

Secondary structure

1
103
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 294Combined sources
Helixi32 – 4110Combined sources
Helixi51 – 7626Combined sources
Beta strandi80 – 823Combined sources
Helixi84 – 9310Combined sources
Beta strandi97 – 1004Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EQZX-ray2.50D/H1-103[»]
1HIOX-ray3.10D28-103[»]
1HQ3X-ray2.15D/H1-103[»]
1TZYX-ray1.90D/H1-103[»]
2AROX-ray2.10D/H1-103[»]
2HIOX-ray3.10D1-103[»]
3C9Kelectron microscopy20.00D/H2-103[»]
ProteinModelPortaliP62801.
SMRiP62801. Positions 21-102.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62801.

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H4 family.Curated

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00760000119019.
HOVERGENiHBG051878.
InParanoidiP62801.
KOiK11254.
PhylomeDBiP62801.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR001951. Histone_H4.
IPR019809. Histone_H4_CS.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00623. HISTONEH4.
SMARTiSM00417. H4. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00047. HISTONE_H4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62801-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL
60 70 80 90 100
IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG

FGG
Length:103
Mass (Da):11,367
Last modified:January 23, 2007 - v2
Checksum:iA9E5DFD3F8B97598
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02218 Genomic DNA. Translation: CAA26137.1.
X02218 Genomic DNA. Translation: CAA26140.1.
J00866 Genomic DNA. No translation available.
M74533 Genomic DNA. Translation: AAA73091.1.
M74534 Genomic DNA. Translation: AAA73092.1.
U37575 Genomic DNA. Translation: AAC59999.1.
U37575 Genomic DNA. Translation: AAC60001.1.
PIRiA02640. HSCH4.
JH0507.
RefSeqiNP_001032932.1. NM_001037843.1.
NP_001032934.1. NM_001037845.1.
NP_001268414.1. NM_001281485.1.
XP_001233095.1. XM_001233094.3.
XP_001233180.1. XM_001233179.3.
XP_003640418.1. XM_003640370.2.
XP_004937726.1. XM_004937669.1.
XP_425458.2. XM_425458.4.
UniGeneiGga.39882.

Genome annotation databases

EnsembliENSGALT00000046233; ENSGALP00000041526; ENSGALG00000025786.
GeneIDi100858049.
100858319.
417946.
417950.
427884.
770005.
770079.
770142.
KEGGigga:100858049.
gga:100858319.
gga:417946.
gga:417950.
gga:427884.
gga:770005.
gga:770079.
gga:770142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02218 Genomic DNA. Translation: CAA26137.1 .
X02218 Genomic DNA. Translation: CAA26140.1 .
J00866 Genomic DNA. No translation available.
M74533 Genomic DNA. Translation: AAA73091.1 .
M74534 Genomic DNA. Translation: AAA73092.1 .
U37575 Genomic DNA. Translation: AAC59999.1 .
U37575 Genomic DNA. Translation: AAC60001.1 .
PIRi A02640. HSCH4.
JH0507.
RefSeqi NP_001032932.1. NM_001037843.1.
NP_001032934.1. NM_001037845.1.
NP_001268414.1. NM_001281485.1.
XP_001233095.1. XM_001233094.3.
XP_001233180.1. XM_001233179.3.
XP_003640418.1. XM_003640370.2.
XP_004937726.1. XM_004937669.1.
XP_425458.2. XM_425458.4.
UniGenei Gga.39882.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EQZ X-ray 2.50 D/H 1-103 [» ]
1HIO X-ray 3.10 D 28-103 [» ]
1HQ3 X-ray 2.15 D/H 1-103 [» ]
1TZY X-ray 1.90 D/H 1-103 [» ]
2ARO X-ray 2.10 D/H 1-103 [» ]
2HIO X-ray 3.10 D 1-103 [» ]
3C9K electron microscopy 20.00 D/H 2-103 [» ]
ProteinModelPortali P62801.
SMRi P62801. Positions 21-102.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 679227. 6 interactions.
IntActi P62801. 2 interactions.
STRINGi 9031.ENSGALP00000037292.

Proteomic databases

PaxDbi P62801.
PRIDEi P62801.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000046233 ; ENSGALP00000041526 ; ENSGALG00000025786 .
GeneIDi 100858049.
100858319.
417946.
417950.
427884.
770005.
770079.
770142.
KEGGi gga:100858049.
gga:100858319.
gga:417946.
gga:417950.
gga:427884.
gga:770005.
gga:770079.
gga:770142.

Organism-specific databases

CTDi 100858049.
100858319.
417946.
417950.
427884.
770005.
770079.
770142.

Phylogenomic databases

eggNOGi COG2036.
GeneTreei ENSGT00760000119019.
HOVERGENi HBG051878.
InParanoidi P62801.
KOi K11254.
PhylomeDBi P62801.

Enzyme and pathway databases

Reactomei REACT_189773. RNA Polymerase I Chain Elongation.
REACT_194991. Oxidative Stress Induced Senescence.
REACT_197049. Amyloids.
REACT_197522. Meiotic recombination.
REACT_197579. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_197820. HATs acetylate histones.
REACT_198068. Condensation of Prophase Chromosomes.
REACT_204005. PRC2 methylates histones and DNA.
REACT_214700. Senescence-Associated Secretory Phenotype (SASP).
REACT_223091. SIRT1 negatively regulates rRNA Expression.
REACT_223355. NoRC negatively regulates rRNA expression.
REACT_224340. RNA Polymerase I Promoter Opening.

Miscellaneous databases

EvolutionaryTracei P62801.
NextBioi 20821175.
PROi P62801.

Gene expression databases

ExpressionAtlasi P62801. baseline and differential.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR001951. Histone_H4.
IPR019809. Histone_H4_CS.
[Graphical view ]
Pfami PF00125. Histone. 1 hit.
[Graphical view ]
PRINTSi PR00623. HISTONEH4.
SMARTi SM00417. H4. 1 hit.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
PROSITEi PS00047. HISTONE_H4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Inverted duplication of histone genes in chicken and disposition of regulatory sequences."
    Wang S.W., Robins A.J., D'Andrea R., Wells J.R.E.
    Nucleic Acids Res. 13:1369-1387(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genomic organization, DNA sequence, and expression of chicken embryonic histone genes."
    Sugarman B.J., Dodgson J.B., Engel J.D.
    J. Biol. Chem. 258:9005-9016(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Nucleotide sequences of two members of the chicken H4 histone-encoding gene family."
    Nakayama T., Takechi S., Ohshige T., Kondo K., Yamamoto K.
    Gene 108:311-312(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (H4-III AND H4-IV).
  4. "Organization of the chicken histone genes in a major gene cluster and generation of an almost complete set of the core histone protein sequences."
    Takami Y., Higashio M., Fukuoka T., Takechi S., Nakayama T.
    DNA Res. 3:95-99(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (H4-VI AND H4-VII).
    Strain: White leghorn.
    Tissue: Liver.
  5. "The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix."
    Arents G., Burlingame R.W., Wang B.-C., Love W.E., Moudrianakis E.N.
    Proc. Natl. Acad. Sci. U.S.A. 88:10148-10152(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
  6. "Asymmetries in the nucleosome core particle at 2.5 A resolution."
    Harp J.M., Hanson B.L., Timm D.E., Bunick G.J.
    Acta Crystallogr. D 56:1513-1534(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiH4_CHICK
AccessioniPrimary (citable) accession number: P62801
Secondary accession number(s): P02304, P02305
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3