Histone H4 - Oreochromis niloticus (Nile tilapia)

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P62796 (H4_ORENI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 54. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names	Recommended name: Histone H4
Organism	Oreochromis niloticus (Nile tilapia) (Tilapia nilotica) [Reference proteome]
Taxonomic identifier	8128 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Perciformes › Labroidei › Cichlidae › African cichlids › Pseudocrenilabrinae › Oreochromini › Oreochromis

Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subunit structure	The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular location	Nucleus By similarity. Chromosome By similarity.
Post-translational modification	Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin By similarity. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation By similarity. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage By similarity. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing By similarity. Phosphorylated by pak2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4 By similarity. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me) By similarity. Sumoylated, which is associated with transcriptional repression By similarity.
Sequence similarities	Belongs to the histone H4 family.

Ontologies

Keywords
Cellular component	Chromosome Nucleosome core Nucleus
Ligand	DNA-binding
PTM	Acetylation Citrullination Isopeptide bond Methylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleosome assembly Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleosome Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 103

102

Histone H4

PRO_0000158339

Regions

DNA binding

17 – 21

Amino acid modifications

Modified residue

N-acetylserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Asymmetric dimethylarginine; by PRMT1; alternate By similarity

Modified residue

Citrulline; alternate By similarity

Modified residue

Omega-N-methylarginine; by PRMT1; alternate By similarity

Modified residue

Symmetric dimethylarginine; by PRMT5 and PRMT7; alternate By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6,N6,N6-trimethyllysine; alternate By similarity

Modified residue

N6,N6-dimethyllysine; alternate By similarity

Modified residue

N6-methyllysine; alternate By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

Phosphoserine; by PAK2 By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

N6-acetyllysine; alternate By similarity

Cross-link

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P62796 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: A9E5DFD3F8B97598
Show »

FASTA

103

11,367

        10         20         30         40         50         60 
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK 

        70         80         90        100 
VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG

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References

[1]

"An unusual histone H4 gene from Tilapia nilotica exhibiting characteristics of both a replication-dependent histone and a basal-expression histone: evolutionary considerations."
Englander E., Moav B., Tabachnik M., Shuali Y., Graur D.
J. Evol. Biol. 6:129-137(1993)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54078 Genomic DNA. Translation: CAA38015.1.
RefSeq	XP_003448793.1. XM_003448745.1. XP_003451199.1. XM_003451151.1. XP_003451208.1. XM_003451160.1. XP_003451210.1. XM_003451162.1. XP_003451213.1. XM_003451165.1. XP_003451217.1. XM_003451169.1. XP_003454720.1. XM_003454672.1. XP_003457429.1. XM_003457381.1. XP_003458488.1. XM_003458440.1. XP_003459140.1. XM_003459092.1. XP_003459575.1. XM_003459527.1. XP_003459577.1. XM_003459529.1. XP_003459580.1. XM_003459532.1. XP_003460247.1. XM_003460199.1.
3D structure databases
ProteinModelPortal	P62796.
ModBase	Search...
Proteomic databases
PRIDE	P62796.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100691120. 100691659. 100692464. 100693547. 100694299. 100694836. 100695624. 100700428. 100702755. 100702942. 100707196. 100707512. 100711673. 100712429.
Phylogenomic databases
HOVERGEN	HBG051878.
OMA	TAMDIVY.
Family and domain databases
Gene3D	1.10.20.10. 1 hit.
InterPro	IPR009072. Histone-fold. IPR007125. Histone_core_D. IPR001951. Histone_H4. IPR019809. Histone_H4_CS. [Graphical view]
PANTHER	PTHR10484. PTHR10484. 1 hit.
Pfam	PF00125. Histone. 1 hit. [Graphical view]
PRINTS	PR00623. HISTONEH4.
SMART	SM00417. H4. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
PROSITE	PS00047. HISTONE_H4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H4_ORENI

Accession

Primary (citable) accession number: P62796
Secondary accession number(s): P02304, P02305

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents