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Protein

Myotrophin

Gene

Mtpn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of the growth of actin filaments. Inhibits the activity of the F-actin-capping protein complex formed by the CAPZA1 and CAPZB heterodimer (By similarity). Promotes dimerization of NF-kappa-B subunits and regulates NF-kappa-B transcription factor activity. Promotes growth of cardiomyocytes, but not cardiomyocyte proliferation. Promotes cardiac muscle hypertrophy.By similarity2 Publications

GO - Molecular functioni

  • sequence-specific DNA binding Source: RGD

GO - Biological processi

  • catecholamine metabolic process Source: RGD
  • cell growth Source: Ensembl
  • cellular response to mechanical stimulus Source: RGD
  • cerebellar granule cell differentiation Source: RGD
  • neuron differentiation Source: UniProtKB
  • positive regulation of cardiac muscle hypertrophy Source: UniProtKB
  • positive regulation of cell growth Source: UniProtKB
  • positive regulation of macromolecule biosynthetic process Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of protein metabolic process Source: UniProtKB
  • regulation of barbed-end actin filament capping Source: UniProtKB
  • skeletal muscle tissue regeneration Source: RGD
  • striated muscle cell differentiation Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Myotrophin
Alternative name(s):
Granule cell differentiation protein
Protein V-1
Gene namesi
Name:Mtpn
Synonyms:Gcdp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi619806. Mtpn.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: RGD
  • cytosol Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • F-actin capping protein complex Source: Ensembl
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi33 – 331E → A: Reduced interaction with RELA. Reduced translocation to the nucleus. Reduced activation of NF-kappa-B transcription factor activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 118117MyotrophinPRO_0000067033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylcysteine1 Publication
Modified residuei4 – 41N6-acetyllysineBy similarity
Modified residuei11 – 111N6-acetyllysineBy similarity
Modified residuei24 – 241N6-acetyllysineBy similarity
Modified residuei31 – 311PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62775.
PRIDEiP62775.

PTM databases

iPTMnetiP62775.

Expressioni

Developmental stagei

At the completion of differentiation and migration of granular cells and at the initiation of the formation of synapses in cerebellar neurons.

Gene expression databases

GenevisibleiP62775. RN.

Interactioni

Subunit structurei

Interacts with the heterodimer formed by CAPZA1 and CAPZB (By similarity). Interacts with RELA.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CapzbQ5XI323EBI-2128047,EBI-2128068

Protein-protein interaction databases

IntActiP62775. 1 interaction.
MINTiMINT-254002.
STRINGi10116.ENSRNOP00000015808.

Structurei

Secondary structure

1
118
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 119Combined sources
Helixi15 – 228Combined sources
Turni23 – 253Combined sources
Beta strandi32 – 354Combined sources
Helixi40 – 456Combined sources
Turni47 – 493Combined sources
Helixi50 – 567Combined sources
Turni60 – 623Combined sources
Beta strandi65 – 673Combined sources
Helixi71 – 766Combined sources
Helixi82 – 887Combined sources
Beta strandi94 – 1007Combined sources
Helixi102 – 1065Combined sources
Turni111 – 1133Combined sources
Helixi114 – 1174Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MYONMR-A1-118[»]
2MYONMR-A1-118[»]
ProteinModelPortaliP62775.
SMRiP62775. Positions 1-118.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62775.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 3029ANK 1Add
BLAST
Repeati34 – 6633ANK 2Add
BLAST
Repeati67 – 9933ANK 3Add
BLAST

Sequence similaritiesi

Belongs to the myotrophin family.Curated
Contains 3 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG4214. Eukaryota.
ENOG4111U90. LUCA.
GeneTreeiENSGT00430000031071.
HOGENOMiHOG000276399.
HOVERGENiHBG019067.
InParanoidiP62775.
OMAiTALIDCT.
OrthoDBiEOG7SBNQP.
PhylomeDBiP62775.
TreeFamiTF327387.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDKEFMWAL KNGDLDEVKD YVAKGEDVNR TLEGGRKPLH YAADCGQLEI
60 70 80 90 100
LEFLLLKGAD INAPDKHHIT PLLSAVYEGH VSCVKLLLSK GADKTVKGPD
110
GLTALEATDN QAIKALLQ
Length:118
Mass (Da):12,861
Last modified:January 23, 2007 - v2
Checksum:i9097FFDF61DB8BA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26179 mRNA. Translation: BAA05167.1.
U21661 mRNA. Translation: AAC52498.1.
AY951952 mRNA. Translation: AAX54865.1.
BC088136 mRNA. Translation: AAH88136.1.
PIRiA54412.
RefSeqiNP_077350.1. NM_024374.4.
UniGeneiRn.3239.

Genome annotation databases

EnsembliENSRNOT00000015808; ENSRNOP00000015808; ENSRNOG00000011857.
GeneIDi79215.
KEGGirno:79215.
UCSCiRGD:619806. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26179 mRNA. Translation: BAA05167.1.
U21661 mRNA. Translation: AAC52498.1.
AY951952 mRNA. Translation: AAX54865.1.
BC088136 mRNA. Translation: AAH88136.1.
PIRiA54412.
RefSeqiNP_077350.1. NM_024374.4.
UniGeneiRn.3239.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MYONMR-A1-118[»]
2MYONMR-A1-118[»]
ProteinModelPortaliP62775.
SMRiP62775. Positions 1-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62775. 1 interaction.
MINTiMINT-254002.
STRINGi10116.ENSRNOP00000015808.

PTM databases

iPTMnetiP62775.

Proteomic databases

PaxDbiP62775.
PRIDEiP62775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015808; ENSRNOP00000015808; ENSRNOG00000011857.
GeneIDi79215.
KEGGirno:79215.
UCSCiRGD:619806. rat.

Organism-specific databases

CTDi136319.
RGDi619806. Mtpn.

Phylogenomic databases

eggNOGiKOG4214. Eukaryota.
ENOG4111U90. LUCA.
GeneTreeiENSGT00430000031071.
HOGENOMiHOG000276399.
HOVERGENiHBG019067.
InParanoidiP62775.
OMAiTALIDCT.
OrthoDBiEOG7SBNQP.
PhylomeDBiP62775.
TreeFamiTF327387.

Miscellaneous databases

EvolutionaryTraceiP62775.
PROiP62775.

Gene expression databases

GenevisibleiP62775. RN.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine cerebellar neurons express a novel gene encoding a protein related to cell cycle control and cell fate determination proteins."
    Taoka M., Isobe T., Okuyama T., Watanabe M., Kondo H., Yamakawa Y., Ozawa F., Hishinuma F., Kubota M., Minegishi A., Song S.-Y., Yamakuni T.
    J. Biol. Chem. 269:9946-9951(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "Cardiac myotrophin exhibits rel/NF-kappa B interacting activity in vitro."
    Sivasubramanian N.C., Adhikary G., Sil P.C., Sen S.
    J. Biol. Chem. 271:2812-2816(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  3. "Characterization and functional significance of myotrophin: a gene with multiple transcripts."
    Adhikary G., Gupta S., Sil P., Saad Y., Sen S.
    Gene 353:31-40(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  5. Cited for: PROTEIN SEQUENCE OF 2-118, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT CYS-2.
    Strain: Wistar.
    Tissue: Brain.
  6. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 37-57 AND 67-85, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  7. "Myotrophin/V-1 does not act as an extracellular signal to induce myocyte hypertrophy."
    Knuefermann P., Shi S.P., Chen P., Sakata Y., Baumgarten G., Sivasubramanian N.
    Tex. Heart Inst. J. 33:281-289(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  8. "Nuclear co-translocation of myotrophin and p65 stimulates myocyte growth. Regulation by myotrophin hairpin loops."
    Das B., Gupta S., Vasanji A., Xu Z., Misra S., Sen S.
    J. Biol. Chem. 283:27947-27956(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RELA, MUTAGENESIS OF GLU-33, FUNCTION.
  9. "Nuclear magnetic resonance assignment and secondary structure of an ankyrin-like repeat-bearing protein: myotrophin."
    Yang Y., Rao N.S., Walker E., Sen S., Qin J.
    Protein Sci. 6:1347-1351(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  10. "The structural basis of ankyrin-like repeat function as revealed by the solution structure of myotrophin."
    Yang Y., Nanduri S., Sen S., Qin J.
    Structure 6:619-626(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiMTPN_RAT
AccessioniPrimary (citable) accession number: P62775
Secondary accession number(s): P80144, Q58HB3, Q9DCN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.