P62756 (IATR_CAPHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Inter-alpha-trypsin inhibitor Short name=ITI Alternative name(s): GIK-14 Inhibitory fragment of ITI
Organism	Capra hircus (Goat)
Taxonomic identifier	9925 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Capra

Protein attributes

Sequence length	123 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This inhibitory fragment, released from native ITI after limited proteolysis with trypsin, contains two homologous domains. Whereas the second domain is a strong inhibitor of trypsin, the first domain interacts weakly with PMN-granulocytic elastase and not at all with pancreatic elastase.
Subcellular location	Secreted.
Miscellaneous	The amino acid at position p2' (17) appears to determine the specificity of the inhibition of domain I. Inhibitors with methionine in this position interact weakly with chymotrypsin and elastase; those with leucine interact strongly.
Sequence similarities	Contains 2 BPTI/Kunitz inhibitor domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›123	›123	Inter-alpha-trypsin inhibitor		PRO_0000155404
Regions
	Domain	5 – 55	51	BPTI/Kunitz inhibitor 1
	Domain	61 – 111	51	BPTI/Kunitz inhibitor 2
Sites
	Site	15 – 16	2	Reactive bond for chymotrypsin and elastase
	Site	71 – 72	2	Reactive bond for trypsin
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...)
	Disulfide bond	5 ↔ 55		By similarity
	Disulfide bond	14 ↔ 38		By similarity
	Disulfide bond	30 ↔ 51		By similarity
	Disulfide bond	61 ↔ 111		By similarity
	Disulfide bond	70 ↔ 94		By similarity
	Disulfide bond	86 ↔ 107		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	123	1

Sequences

	Sequence		Length	Mass (Da)
	P62756 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 295038173F22D2D1	FASTA	123	13,687
	10 20 30 40 50 60 KEDSCQLGYS QGPCLGMFKR YFYNGTSMAC ETFYYGGCMG NGNNFPSEKE CLQTCRTVQA 70 80 90 100 110 120 CNLPIVRGPC RAGIELWAFD AVKGKCVRFI YGGCNGNGNQ FYSQKECKEY CGIPGEADEE LLR

References

[1]	"Primary structure of a proteinase inhibitor released from goat serum inter-alpha-trypsin inhibitor." Rasp G., Hochstrasser K., Gerl C., Wachter E. Biochim. Biophys. Acta 999:335-337(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
ProteinModelPortal	P62756.
SMR	P62756. Positions 4-113.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG056804.
Family and domain databases
Gene3D	4.10.410.10. 2 hits.
InterPro	IPR002223. Prot_inh_Kunz-m. IPR020901. Prtase_inh_Kunz-CS. [Graphical view]
Pfam	PF00014. Kunitz_BPTI. 2 hits. [Graphical view]
PRINTS	PR00759. BASICPTASE.
SMART	SM00131. KU. 2 hits. [Graphical view]
SUPFAM	SSF57362. Prot_inh_Kunz-m. 2 hits.
PROSITE	PS00280. BPTI_KUNITZ_1. 2 hits. PS50279. BPTI_KUNITZ_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IATR_CAPHI

Accession

Primary (citable) accession number: P62756
Secondary accession number(s): P13371

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program