P62753 (RS6_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 87.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 40S ribosomal protein S6 Alternative name(s): Phosphoprotein NP33 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 249 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. |
| Post-translational modification | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.20 |
| Sequence similarities | Belongs to the ribosomal protein S6e family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| NCBP1 | Q09161 | 3 | EBI-356625,EBI-464743 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 249 | 249 | 40S ribosomal protein S6 | PRO_0000137312 | |||||
Amino acid modifications | |||||||||
| Modified residue | 30 | 1 | N6-acetyllysine Ref.18 | ||||||
| Modified residue | 78 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 148 | 1 | Phosphoserine Ref.15 | ||||||
| Modified residue | 211 | 1 | N6-acetyllysine Ref.18 | ||||||
| Modified residue | 235 | 1 | Phosphoserine; by RPS6KA1, RPS6KA3, DAPK1 and PASK Ref.8 Ref.9 Ref.10 Ref.11 Ref.15 Ref.16 Ref.17 Ref.20 | ||||||
| Modified residue | 236 | 1 | Phosphoserine; by RPS6KA1, RPS6KA3, DAPK1 and PASK Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.15 Ref.16 Ref.20 | ||||||
| Modified residue | 240 | 1 | Phosphoserine Ref.9 Ref.11 Ref.12 Ref.14 Ref.15 Ref.17 | ||||||
| Modified residue | 244 | 1 | Phosphoserine Ref.11 Ref.15 | ||||||
| Modified residue | 246 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 247 | 1 | Phosphoserine Ref.15 | ||||||
Natural variations | |||||||||
| Natural variant | 221 | 1 | K → R. Ref.6 Corresponds to variant rs17852447 [ dbSNP | Ensembl ]. | VAR_025314 | |||||
Experimental info | |||||||||
| Mutagenesis | 235 – 236 | 2 | SS → AA: Abolishes pohsphorylation by PASK. Ref.20 | ||||||
| Sequence conflict | 23 | 1 | K → T in AAA60288. Ref.1 | ||||||
| Sequence conflict | 144 | 1 | L → R in AAA60288. Ref.1 | ||||||
| Sequence conflict | 155 – 156 | 2 | QY → EC in AAA60287. Ref.2 | ||||||
| Sequence conflict | 168 | 1 | K → R in AAA60287. Ref.2 | ||||||
| Sequence conflict | 196 | 1 | K → Q in AAA60288. Ref.1 | ||||||
| Sequence conflict | 219 | 1 | E → Q in AAA60287. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6." Lott J.B., Mackie G.A. Gene 65:31-39(1988) [PubMed: 2840355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The primary structure of the human ribosomal protein S6 derived from a cloned cDNA." Heinze H., Arnold H.H., Fischer D., Kruppa J. J. Biol. Chem. 263:4139-4144(1988) [PubMed: 3279029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The organization of the intron-containing human S6 ribosomal protein (rpS6) gene and determination of its location at chromosome 9p21." Antoine M., Fried M. Hum. Mol. Genet. 1:565-570(1992) [PubMed: 1301164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "The human ribosomal protein S6 gene: isolation, primary structure and location in chromosome 9." Pata I., Hoth S., Kruppa J., Metspalu A. Gene 121:387-392(1992) [PubMed: 1446836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [5] | "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon adenocarcinoma. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-221. Tissue: Colon, Muscle, Pancreas, Skin and Testis. |
| [7] | "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry." Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K., Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A. Eur. J. Biochem. 239:144-149(1996) [PubMed: 8706699] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15. Tissue: Placenta. |
| [8] | "Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235 AND SER-236, MASS SPECTROMETRY. Tissue: Colon adenocarcinoma. |
| [9] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235; SER-236 AND SER-240, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [10] | "RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation." Roux P.P., Shahbazian D., Vu H., Holz M.K., Cohen M.S., Taunton J., Sonenberg N., Blenis J. J. Biol. Chem. 282:14056-14064(2007) [PubMed: 17360704] [Abstract] Cited for: PHOSPHORYLATION AT SER-235 AND SER-236. |
| [11] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78; SER-235; SER-236; SER-240 AND SER-244, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [12] | "Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [13] | "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [14] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [15] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148; SER-235; SER-236; SER-240; SER-244 AND SER-247, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [16] | "Peptide combinatorial libraries identify TSC2 as a death-associated protein kinase (DAPK) death domain-binding protein and reveal a stimulatory role for DAPK in mTORC1 signaling." Stevens C., Lin Y., Harrison B., Burch L., Ridgway R.A., Sansom O., Hupp T. J. Biol. Chem. 284:334-344(2009) [PubMed: 18974095] [Abstract] Cited for: PHOSPHORYLATION AT SER-235 AND SER-236 BY DAPK1. |
| [17] | "Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235 AND SER-240, MASS SPECTROMETRY. |
| [18] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-30 AND LYS-211, MASS SPECTROMETRY. |
| [19] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [20] | "Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN." Schlafli P., Troger J., Eckhardt K., Borter E., Spielmann P., Wenger R.H. FEBS J. 278:1757-1768(2011) [PubMed: 21418524] [Abstract] Cited for: PHOSPHORYLATION AT SER-235 AND SER-236, MUTAGENESIS OF 235-SER-SER-236. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M20020 mRNA. Translation: AAA60288.1. J03537 mRNA. Translation: AAA60287.1. X67309 Genomic DNA. Translation: CAA47719.1. M77232 Genomic DNA. Translation: AAA60289.1. AB062123 mRNA. Translation: BAB93455.1. BC000524 mRNA. Translation: AAH00524.1. BC009427 mRNA. Translation: AAH09427.2. BC027620 mRNA. Translation: AAH27620.1. BC071907 mRNA. Translation: AAH71907.1. BC071908 mRNA. Translation: AAH71908.1. BC094826 mRNA. Translation: AAH94826.1. |
| IPI | IPI00021840. |
| PIR | R3HU6. JC1394. |
| RefSeq | NP_001001.2. NM_001010.2. |
| UniGene | Hs.408073. |
3D structure databases | |
| ProteinModelPortal | P62753. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-31507N. |
| IntAct | P62753. 22 interactions. |
| MINT | MINT-1162101. |
| STRING | P62753. |
PTM databases | |
| PhosphoSite | P62753. |
Polymorphism databases | |
| DMDM | 51338632. |
Proteomic databases | |
| PRIDE | P62753. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000380394; ENSP00000369757; ENSG00000137154. |
| GeneID | 6194. |
| KEGG | hsa:6194. |
| UCSC | uc003znv.1. human. |
Organism-specific databases | |
| CTD | 6194. |
| GeneCards | GC09M019366. |
| H-InvDB | HIX0007947. |
| HGNC | HGNC:10429. RPS6. |
| HPA | CAB004027. HPA031153. |
| MIM | 180460. gene. |
| neXtProt | NX_P62753. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG316337. |
| HOVERGEN | HBG011441. |
| InParanoid | P62753. |
| OMA | VFYEKRM. |
| OrthoDB | EOG4KD6N0. |
| PhylomeDB | P62753. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | il2_pi3kpathway. IL2 signaling events mediated by PI3K. mtor_4pathway. mTOR signaling pathway. |
| Reactome | REACT_111045. Developmental Biology. REACT_111102. Signal Transduction. REACT_111217. Metabolism. REACT_15380. Diabetes pathways. REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_6167. Influenza Infection. REACT_71. Gene Expression. |
Gene expression databases | |
| ArrayExpress | P62753. |
| Bgee | P62753. |
| CleanEx | HS_RPS6. |
| Genevestigator | P62753. |
| GermOnline | ENSG00000137154. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR014401. Ribosomal_S6_euk. IPR001377. Ribosomal_S6e. IPR018282. Ribosomal_S6e_CS. [Graphical view] |
| KO | K02991. |
| PANTHER | PTHR11502. Ribosomal_S6E. 1 hit. |
| Pfam | PF01092. Ribosomal_S6e. 1 hit. [Graphical view] |
| PIRSF | PIRSF002129. Ribosom_S6_euk. 1 hit. |
| PROSITE | PS00578. RIBOSOMAL_S6E. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 24053. |
| SOURCE | Search... |
Entry information
| Entry name | RS6_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P62753 Secondary accession number(s): P08227 Q8N6Z7 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Ribosomal proteins Ribosomal proteins families and list of entries |
| Human chromosome 9 Human chromosome 9: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with