Reviewed, UniProtKB/Swiss-Prot P62753 (RS6_HUMAN)

Names and origin

Protein names

Recommended name:
    40S ribosomal protein S6
Alternative name(s):
    Phosphoprotein NP33

Gene names

Name:	RPS6
ORF Names:	OK/SW-cl.2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Post-translational modification	Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
Sequence similarities	Belongs to the ribosomal protein S6e family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	TOR signaling pathway Inferred from direct assay. Source: UniProtKB glucose homeostasis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptosis Inferred from direct assay. Source: UniProtKB rRNA processing Inferred from mutant phenotype. Source: UniProtKB ribosomal small subunit biogenesis Inferred from mutant phenotype. Source: UniProtKB translational elongation Inferred from Experiment. Source: Reactome
Cellular component	cytosolic small ribosomal subunit Ref.3 Inferred from direct assay. Source: UniProtKB nucleolus Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCBP1	Q09161	2	EBI-356625,EBI-464743
NDRG1	Q92597	1	EBI-356625,EBI-716486

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	40S ribosomal protein S6		PRO_0000137312
Amino acid modifications
	Modified residue	78	1	Phosphoserine
	Modified residue	148	1	Phosphoserine
	Modified residue	235	1	Phosphoserine
	Modified residue	236	1	Phosphoserine
	Modified residue	240	1	Phosphoserine
	Modified residue	244	1	Phosphoserine
	Modified residue	246	1	Phosphoserine By similarity
	Modified residue	247	1	Phosphoserine
Natural variations
	Natural variant	221	1	K → R: dbSNP rs17852447.		VAR_025314
Experimental info
	Sequence conflict	23	1	K → T in AAA60288. Ref.1
	Sequence conflict	144	1	L → R in AAA60288. Ref.1
	Sequence conflict	155 – 156	2	QY → EC in AAA60287. Ref.2
	Sequence conflict	168	1	K → R in AAA60287. Ref.2
	Sequence conflict	196	1	K → Q in AAA60288. Ref.1
	Sequence conflict	219	1	E → Q in AAA60287. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P62753-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: A61E435884E636AE	FASTA	249	28,681
	10 20 30 40 50 60 MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG 70 80 90 100 110 120 FPMKQGVLTH GRVRLLLSKG HSCYRPRRTG ERKRKSVRGC IVDANLSVLN LVIVKKGEKD 130 140 150 160 170 180 IPGLTDTTVP RRLGPKRASR IRKLFNLSKE DDVRQYVVRK PLNKEGKKPR TKAPKIQRLV 190 200 210 220 230 240 TPRVLQHKRR RIALKKQRTK KNKEEAAEYA KLLAKRMKEA KEKRQEQIAK RRRLSSLRAS TSKSESSQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6." Lott J.B., Mackie G.A. Gene 65:31-39(1988) [PubMed: 2840355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The primary structure of the human ribosomal protein S6 derived from a cloned cDNA." Heinze H., Arnold H.H., Fischer D., Kruppa J. J. Biol. Chem. 263:4139-4144(1988) [PubMed: 3279029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The organization of the intron-containing human S6 ribosomal protein (rpS6) gene and determination of its location at chromosome 9p21." Antoine M., Fried M. Hum. Mol. Genet. 1:565-570(1992) [PubMed: 1301164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The human ribosomal protein S6 gene: isolation, primary structure and location in chromosome 9." Pata I., Hoth S., Kruppa J., Metspalu A. Gene 121:387-392(1992) [PubMed: 1446836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon adenocarcinoma.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-221. Tissue: Colon, Muscle, Pancreas, Skin and Testis.
[7]	"Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry." Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K., Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A. Eur. J. Biochem. 239:144-149(1996) [PubMed: 8706699] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15. Tissue: Placenta.
[8]	"Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235 AND SER-236, MASS SPECTROMETRY.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235; SER-236 AND SER-240, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78; SER-235; SER-236; SER-240 AND SER-244, MASS SPECTROMETRY.
[11]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148; SER-235; SER-236; SER-240; SER-244 AND SER-247, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20020 mRNA. Translation: AAA60288.1. J03537 mRNA. Translation: AAA60287.1. X67309 Genomic DNA. Translation: CAA47719.1. M77232 Genomic DNA. Translation: AAA60289.1. AB062123 mRNA. Translation: BAB93455.1. BC000524 mRNA. Translation: AAH00524.1. BC009427 mRNA. Translation: AAH09427.2. BC027620 mRNA. Translation: AAH27620.1. BC071907 mRNA. Translation: AAH71907.1. BC071908 mRNA. Translation: AAH71908.1. BC094826 mRNA. Translation: AAH94826.1.
PIR	R3HU6. JC1394.
RefSeq	NP_001001.2.
UniGene	Hs.408073
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P62753.
PTM databases
PhosphoSite	P62753.
Genome annotation databases
Ensembl	ENSG00000137154. Homo sapiens. [Contig view]
GeneID	6194.
KEGG	hsa:6194.
Organism-specific databases
H-InvDB	HIX0007947.
HGNC	HGNC:10429. RPS6.
HPA	CAB004027.
MIM	180460. gene.
PharmGKB	PA31025.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P62753.
HOVERGEN	P62753.
Enzyme and pathway databases
Reactome	REACT_1762. 3' -UTR-mediated translational regulation. REACT_498. Signaling by insulin receptor. REACT_6167. Influenza Infection. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P62753.
CleanEx	HS_RPS6.
GermOnline	ENSG00000137154. Homo sapiens.
Family and domain databases
InterPro	IPR014401. Ribosomal_S6_euk. IPR001377. Ribosomal_S6e. [Graphical view]
PANTHER	PTHR11502. Ribosomal_S6E. 1 hit.
Pfam	PF01092. Ribosomal_S6e. 1 hit. [Graphical view]
PIRSF	PIRSF002129. Ribosom_S6_euk. 1 hit.
ProDom	PD003460. Ribosomal_S6E. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00578. RIBOSOMAL_S6E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P62753.
NextBio	24053.
SOURCE	Search...

Entry information

Entry name

RS6_HUMAN

Accession

Primary (citable) accession number: P62753
Secondary accession number(s): P08227 , P10660, Q4VBY7, Q8N6Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)