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P62746

- RHOB_MOUSE

UniProt

P62746 - RHOB_MOUSE

Protein

Rho-related GTP-binding protein RhoB

Gene

Rhob

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 198GTPBy similarity
    Nucleotide bindingi59 – 635GTPBy similarity
    Nucleotide bindingi117 – 1204GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: MGI
    2. GTP binding Source: UniProtKB
    3. protein binding Source: MGI

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. cell adhesion Source: UniProtKB
    3. cell differentiation Source: UniProtKB-KW
    4. cellular response to hydrogen peroxide Source: UniProtKB
    5. cellular response to ionizing radiation Source: UniProtKB
    6. cytokinesis Source: UniProtKB
    7. endosome to lysosome transport Source: UniProtKB
    8. negative regulation of cell cycle Source: UniProtKB
    9. positive regulation of angiogenesis Source: UniProtKB
    10. positive regulation of apoptotic process Source: UniProtKB
    11. protein transport Source: UniProtKB-KW
    12. small GTPase mediated signal transduction Source: InterPro
    13. transformed cell apoptotic process Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Angiogenesis, Apoptosis, Cell adhesion, Differentiation, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.
    REACT_210090. Rho GTPase cycle.
    REACT_220092. GPVI-mediated activation cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho-related GTP-binding protein RhoB
    Gene namesi
    Name:Rhob
    Synonyms:Arhb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:107949. Rhob.

    Subcellular locationi

    Late endosome membrane 1 Publication; Lipid-anchor 1 Publication. Cell membrane 1 Publication; Lipid-anchor 1 Publication. Nucleus 1 Publication. Cleavage furrow By similarity
    Note: Translocates to the equatorial region before furrow formation in a ECT2-dependent manner By similarity. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.By similarity

    GO - Cellular componenti

    1. cleavage furrow Source: UniProtKB
    2. cytosol Source: Ensembl
    3. endosome membrane Source: UniProtKB
    4. late endosome membrane Source: UniProtKB-SubCell
    5. membrane Source: MGI
    6. nucleus Source: UniProtKB
    7. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 193193Rho-related GTP-binding protein RhoBPRO_0000030419Add
    BLAST
    Propeptidei194 – 1963Removed in mature formBy similarityPRO_0000030420

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei154 – 1541Phosphotyrosine1 Publication
    Lipidationi189 – 1891S-palmitoyl cysteineBy similarity
    Lipidationi192 – 1921S-palmitoyl cysteineBy similarity
    Modified residuei193 – 1931Cysteine methyl esterBy similarity
    Lipidationi193 – 1931S-farnesyl cysteine; in plasma membrane formBy similarity
    Lipidationi193 – 1931S-geranylgeranyl cysteine; in endosomal formBy similarity

    Post-translational modificationi

    Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiP62746.
    PaxDbiP62746.
    PRIDEiP62746.

    PTM databases

    PhosphoSiteiP62746.

    Expressioni

    Inductioni

    By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation.2 Publications

    Gene expression databases

    ArrayExpressiP62746.
    BgeeiP62746.
    CleanExiMM_RHOB.
    GenevestigatoriP62746.

    Interactioni

    Subunit structurei

    Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13 By similarity. Interacts with RTKN.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP62746. 2 interactions.
    MINTiMINT-2982781.

    Structurei

    3D structure databases

    ProteinModelPortaliP62746.
    SMRiP62746. Positions 2-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi34 – 429Effector regionSequence Analysis

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233974.
    HOVERGENiHBG009351.
    InParanoidiP62746.
    KOiK07856.
    OMAiVWEVFET.
    OrthoDBiEOG73FQPD.
    PhylomeDBiP62746.
    TreeFamiTF300837.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG    50
    KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV 100
    PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR 150
    IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCCKVL 196
    Length:196
    Mass (Da):22,123
    Last modified:August 1, 1988 - v1
    Checksum:iCCE6FD53AE00CD83
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99963 Genomic DNA. Translation: CAA68228.1.
    AF481943 mRNA. Translation: AAL89687.1.
    BC018275 mRNA. Translation: AAH18275.1.
    AK013784 mRNA. Translation: BAB28993.1.
    CCDSiCCDS25799.1.
    PIRiJC5075.
    RefSeqiNP_031509.1. NM_007483.2.
    UniGeneiMm.687.

    Genome annotation databases

    EnsembliENSMUST00000067384; ENSMUSP00000067013; ENSMUSG00000054364.
    GeneIDi11852.
    KEGGimmu:11852.
    UCSCiuc007mzp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99963 Genomic DNA. Translation: CAA68228.1 .
    AF481943 mRNA. Translation: AAL89687.1 .
    BC018275 mRNA. Translation: AAH18275.1 .
    AK013784 mRNA. Translation: BAB28993.1 .
    CCDSi CCDS25799.1.
    PIRi JC5075.
    RefSeqi NP_031509.1. NM_007483.2.
    UniGenei Mm.687.

    3D structure databases

    ProteinModelPortali P62746.
    SMRi P62746. Positions 2-185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P62746. 2 interactions.
    MINTi MINT-2982781.

    PTM databases

    PhosphoSitei P62746.

    Proteomic databases

    MaxQBi P62746.
    PaxDbi P62746.
    PRIDEi P62746.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067384 ; ENSMUSP00000067013 ; ENSMUSG00000054364 .
    GeneIDi 11852.
    KEGGi mmu:11852.
    UCSCi uc007mzp.1. mouse.

    Organism-specific databases

    CTDi 388.
    MGIi MGI:107949. Rhob.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233974.
    HOVERGENi HBG009351.
    InParanoidi P62746.
    KOi K07856.
    OMAi VWEVFET.
    OrthoDBi EOG73FQPD.
    PhylomeDBi P62746.
    TreeFami TF300837.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.
    REACT_210090. Rho GTPase cycle.
    REACT_220092. GPVI-mediated activation cascade.

    Miscellaneous databases

    ChiTaRSi RHOB. mouse.
    NextBioi 1615.
    PROi P62746.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62746.
    Bgeei P62746.
    CleanExi MM_RHOB.
    Genevestigatori P62746.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region."
      Nakamura T., Asano M., Shindo-Okada N., Nishimura S., Monden Y.
      Biochem. Biophys. Res. Commun. 226:688-694(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "RhoB mRNA is stabilized by HuR after UV light."
      Westmark C.J., Bartleson V.B., Malter J.S.
      Oncogene 24:502-511(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Hippocampus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    4. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 8-27, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 160-196.
      Strain: C57BL/6J.
      Tissue: Hippocampus.
    6. "RhoB is dispensable for mouse development, but it modifies susceptibility to tumor formation as well as cell adhesion and growth factor signaling in transformed cells."
      Liu A.-X., Rane N., Liu J.-P., Prendergast G.C.
      Mol. Cell. Biol. 21:6906-6912(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "RhoB is required to mediate apoptosis in neoplastically transformed cells after DNA damage."
      Liu A.-X., Cerniglia G.J., Bernhard E.J., Prendergast G.C.
      Proc. Natl. Acad. Sci. U.S.A. 98:6192-6197(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "RhoB controls Akt trafficking and stage-specific survival of endothelial cells during vascular development."
      Adini I., Rabinovitz I., Sun J.F., Prendergast G.C., Benjamin L.E.
      Genes Dev. 17:2721-2732(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    9. "The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments."
      Fritz G., Kaina B., Aktories K.
      J. Biol. Chem. 270:25172-25177(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain."
      Reid T., Furuyashiki T., Ishizaki T., Watanabe G., Watanabe N., Fujisawa K., Morii N., Madaule P., Narumiya S.
      J. Biol. Chem. 271:13556-13560(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RTKN.
    11. "Mitogen-responsive expression of RhoB is regulated by RNA stability."
      Malcolm T., Ettehadieh E., Sadowski I.
      Oncogene 22:6142-6150(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    12. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiRHOB_MOUSE
    AccessioniPrimary (citable) accession number: P62746
    Secondary accession number(s): P01121, Q9CUV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3