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P62744

- AP2S1_RAT

UniProt

P62744 - AP2S1_RAT

Protein

AP-2 complex subunit sigma

Gene

Ap2s1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis By similarity.By similarity

    GO - Molecular functioni

    1. protein transporter activity Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. intracellular protein transport Source: InterPro

    Keywords - Biological processi

    Endocytosis, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198398. WNT5A-dependent internalization of FZD4.
    REACT_198830. MHC class II antigen presentation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-2 complex subunit sigma
    Alternative name(s):
    Adaptor protein complex AP-2 subunit sigma
    Adaptor-related protein complex 2 subunit sigma
    Clathrin assembly protein 2 sigma small chain
    Clathrin coat assembly protein AP17
    Clathrin coat-associated protein AP17
    Plasma membrane adaptor AP-2 17 kDa protein
    Sigma-adaptin 3b
    Sigma2-adaptin
    Gene namesi
    Name:Ap2s1
    Synonyms:Ap17, Claps2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi620188. Ap2s1.

    Subcellular locationi

    Cell membrane. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side
    Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.By similarity

    GO - Cellular componenti

    1. AP-1 adaptor complex Source: RGD
    2. AP-2 adaptor complex Source: MGI
    3. Golgi apparatus Source: RGD
    4. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Coated pit, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 142142AP-2 complex subunit sigmaPRO_0000193807Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei140 – 1401PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP62744.
    PRIDEiP62744.

    Expressioni

    Gene expression databases

    GenevestigatoriP62744.

    Interactioni

    Subunit structurei

    Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).By similarity

    Protein-protein interaction databases

    IntActiP62744. 1 interaction.
    MINTiMINT-2514729.

    Structurei

    Secondary structure

    1
    142
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Beta strandi14 – 218
    Helixi25 – 4117
    Beta strandi44 – 463
    Beta strandi48 – 514
    Beta strandi53 – 6210
    Beta strandi65 – 728
    Helixi77 – 9519
    Helixi100 – 1056
    Helixi107 – 11711
    Helixi128 – 14013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4NEEX-ray2.88D/F/I/L1-142[»]
    ProteinModelPortaliP62744.
    SMRiP62744. Positions 1-142.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5030.
    GeneTreeiENSGT00530000062839.
    HOGENOMiHOG000185227.
    HOVERGENiHBG050517.
    InParanoidiP62744.
    KOiK11827.
    OMAiSFFDNVC.
    OrthoDBiEOG7S7SGC.
    PhylomeDBiP62744.
    TreeFamiTF300139.

    Family and domain databases

    InterProiIPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR027156. APS2.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view]
    PANTHERiPTHR11753. PTHR11753. 1 hit.
    PTHR11753:SF6. PTHR11753:SF6. 1 hit.
    PfamiPF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRFILIQNR AGKTRLAKWY MQFDDDEKQK LIEEVHAVVT VRDAKHTNFV    50
    EFRNFKIIYR RYAGLYFCIC VDVNDNNLAY LEAIHNFVEV LNEYFHNVCE 100
    LDLVFNFYKV YTVVDEMFLA GEIRETSQTK VLKQLLMLQS LE 142
    Length:142
    Mass (Da):17,018
    Last modified:August 16, 2004 - v1
    Checksum:iCA3FD868C65AEDF6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti83 – 831A → G in AAB46980. (PubMed:10559001)Curated
    Sequence conflicti109 – 1102KV → RF in AAB46980. (PubMed:10559001)Curated
    Sequence conflicti117 – 1171M → I in AAB46980. (PubMed:10559001)Curated
    Sequence conflicti126 – 1261T → R in AAB46980. (PubMed:10559001)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37194 mRNA. Translation: AAA40742.1.
    U75917 mRNA. Translation: AAB46980.1.
    BC088138 mRNA. Translation: AAH88138.1.
    PIRiB40535.
    RefSeqiNP_075241.2. NM_022952.2.
    UniGeneiRn.37866.

    Genome annotation databases

    EnsembliENSRNOT00000021310; ENSRNOP00000021310; ENSRNOG00000015865.
    GeneIDi65046.
    KEGGirno:65046.
    UCSCiRGD:620188. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37194 mRNA. Translation: AAA40742.1 .
    U75917 mRNA. Translation: AAB46980.1 .
    BC088138 mRNA. Translation: AAH88138.1 .
    PIRi B40535.
    RefSeqi NP_075241.2. NM_022952.2.
    UniGenei Rn.37866.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4NEE X-ray 2.88 D/F/I/L 1-142 [» ]
    ProteinModelPortali P62744.
    SMRi P62744. Positions 1-142.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P62744. 1 interaction.
    MINTi MINT-2514729.

    Chemistry

    BindingDBi P62744.
    ChEMBLi CHEMBL1697667.

    Proteomic databases

    PaxDbi P62744.
    PRIDEi P62744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021310 ; ENSRNOP00000021310 ; ENSRNOG00000015865 .
    GeneIDi 65046.
    KEGGi rno:65046.
    UCSCi RGD:620188. rat.

    Organism-specific databases

    CTDi 1175.
    RGDi 620188. Ap2s1.

    Phylogenomic databases

    eggNOGi COG5030.
    GeneTreei ENSGT00530000062839.
    HOGENOMi HOG000185227.
    HOVERGENi HBG050517.
    InParanoidi P62744.
    KOi K11827.
    OMAi SFFDNVC.
    OrthoDBi EOG7S7SGC.
    PhylomeDBi P62744.
    TreeFami TF300139.

    Enzyme and pathway databases

    Reactomei REACT_198398. WNT5A-dependent internalization of FZD4.
    REACT_198830. MHC class II antigen presentation.

    Miscellaneous databases

    NextBioi 381325.
    PROi P62744.

    Gene expression databases

    Genevestigatori P62744.

    Family and domain databases

    InterProi IPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR027156. APS2.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view ]
    PANTHERi PTHR11753. PTHR11753. 1 hit.
    PTHR11753:SF6. PTHR11753:SF6. 1 hit.
    Pfami PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "AP17 and AP19, the mammalian small chains of the clathrin-associated protein complexes show homology to Yap17p, their putative homolog in yeast."
      Kirchhausen T., Davis A.C., Frucht S., O'Brine Greco B., Payne G.S., Tubb B.
      J. Biol. Chem. 266:11153-11157(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "A systematic analysis of 40 random genes in cultured vascular smooth muscle subtypes reveals a heterogeneity of gene expression and identifies the tight junction gene zonula occludens 2 as a marker of epithelioid 'pup' smooth muscle cells and a participant in carotid neointimal formation."
      Adams L.D., Lemire J.M., Schwartz S.M.
      Arterioscler. Thromb. Vasc. Biol. 19:2600-2608(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar Kyoto.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spleen.
    4. "Adaptor protein complexes as the key regulators of protein sorting in the post-Golgi network."
      Nakatsu F., Ohno H.
      Cell Struct. Funct. 28:419-429(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE AP-2 COMPLEX IN CLATHRIN-MEDIATED ENDOCYTOSIS.
    5. "Adaptors for clathrin coats: structure and function."
      Owen D.J., Collins B.M., Evans P.R.
      Annu. Rev. Cell Dev. Biol. 20:153-191(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE AP-2 COMPLEX IN CLATHRIN-MEDIATED ENDOCYTOSIS.

    Entry informationi

    Entry nameiAP2S1_RAT
    AccessioniPrimary (citable) accession number: P62744
    Secondary accession number(s): P70626, P97626, Q00380
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3