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P62743

- AP2S1_MOUSE

UniProt

P62743 - AP2S1_MOUSE

Protein

AP-2 complex subunit sigma

Gene

Ap2s1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis By similarity.By similarity

    GO - Molecular functioni

    1. protein transporter activity Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. intracellular protein transport Source: InterPro

    Keywords - Biological processi

    Endocytosis, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_202778. Retrograde neurotrophin signalling.
    REACT_203917. EGFR downregulation.
    REACT_222404. WNT5A-dependent internalization of FZD4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-2 complex subunit sigma
    Alternative name(s):
    Adaptor protein complex AP-2 subunit sigma
    Adaptor-related protein complex 2 subunit sigma
    Clathrin assembly protein 2 sigma small chain
    Clathrin coat assembly protein AP17
    Clathrin coat-associated protein AP17
    Plasma membrane adaptor AP-2 17 kDa protein
    Sigma-adaptin 3b
    Sigma2-adaptin
    Gene namesi
    Name:Ap2s1
    Synonyms:Ap17, Claps2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2141861. Ap2s1.

    Subcellular locationi

    Cell membrane. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side
    Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.By similarity

    GO - Cellular componenti

    1. AP-2 adaptor complex Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Coated pit, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi15 – 151R → S: Reduces interaction with CD4 endocytosis signal motif; when associated with AP2A2 E-21. 1 Publication
    Mutagenesisi62 – 621Y → S: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi63 – 631A → W: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi65 – 651L → S: Slightly reduces interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi88 – 881V → D: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi92 – 921N → W: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi98 – 981V → F: Reduces interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi98 – 981V → S: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi100 – 1001E → Y: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication
    Mutagenesisi103 – 1031L → S: Abolishes interaction with CD4 endocytosis signal motif. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 142142AP-2 complex subunit sigmaPRO_0000193805Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei140 – 1401PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP62743.
    PaxDbiP62743.
    PRIDEiP62743.

    PTM databases

    PhosphoSiteiP62743.

    Expressioni

    Gene expression databases

    ArrayExpressiP62743.
    BgeeiP62743.
    GenevestigatoriP62743.

    Interactioni

    Subunit structurei

    Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).2 Publications

    Protein-protein interaction databases

    BioGridi231318. 1 interaction.
    DIPiDIP-37505N.
    IntActiP62743. 2 interactions.
    MINTiMINT-1869996.

    Structurei

    Secondary structure

    1
    142
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Beta strandi14 – 196
    Helixi25 – 4016
    Beta strandi44 – 463
    Beta strandi48 – 525
    Beta strandi55 – 628
    Beta strandi65 – 717
    Beta strandi73 – 753
    Helixi77 – 9418
    Helixi100 – 1056
    Helixi107 – 11711
    Helixi128 – 14013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JKRX-ray2.98I/S1-142[»]
    2JKTX-ray3.40I/S1-142[»]
    2VGLX-ray2.59S1-142[»]
    2XA7X-ray3.10S1-142[»]
    ProteinModelPortaliP62743.
    SMRiP62743. Positions 1-142.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62743.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5030.
    HOGENOMiHOG000185227.
    HOVERGENiHBG050517.
    InParanoidiP62743.
    KOiK11827.
    OMAiSFFDNVC.
    OrthoDBiEOG7S7SGC.
    PhylomeDBiP62743.
    TreeFamiTF300139.

    Family and domain databases

    InterProiIPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR027156. APS2.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view]
    PANTHERiPTHR11753. PTHR11753. 1 hit.
    PTHR11753:SF6. PTHR11753:SF6. 1 hit.
    PfamiPF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62743-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRFILIQNR AGKTRLAKWY MQFDDDEKQK LIEEVHAVVT VRDAKHTNFV    50
    EFRNFKIIYR RYAGLYFCIC VDVNDNNLAY LEAIHNFVEV LNEYFHNVCE 100
    LDLVFNFYKV YTVVDEMFLA GEIRETSQTK VLKQLLMLQS LE 142
    Length:142
    Mass (Da):17,018
    Last modified:August 16, 2004 - v1
    Checksum:iCA3FD868C65AEDF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AA277150 mRNA. No translation available.
    CCDSiCCDS52042.1.
    RefSeqiNP_941015.2. NM_198613.2.
    UniGeneiMm.333597.

    Genome annotation databases

    EnsembliENSMUST00000086112; ENSMUSP00000083281; ENSMUSG00000008036.
    GeneIDi232910.
    KEGGimmu:232910.
    UCSCiuc012faj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AA277150 mRNA. No translation available.
    CCDSi CCDS52042.1.
    RefSeqi NP_941015.2. NM_198613.2.
    UniGenei Mm.333597.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JKR X-ray 2.98 I/S 1-142 [» ]
    2JKT X-ray 3.40 I/S 1-142 [» ]
    2VGL X-ray 2.59 S 1-142 [» ]
    2XA7 X-ray 3.10 S 1-142 [» ]
    ProteinModelPortali P62743.
    SMRi P62743. Positions 1-142.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231318. 1 interaction.
    DIPi DIP-37505N.
    IntActi P62743. 2 interactions.
    MINTi MINT-1869996.

    Chemistry

    BindingDBi P62743.

    PTM databases

    PhosphoSitei P62743.

    Proteomic databases

    MaxQBi P62743.
    PaxDbi P62743.
    PRIDEi P62743.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000086112 ; ENSMUSP00000083281 ; ENSMUSG00000008036 .
    GeneIDi 232910.
    KEGGi mmu:232910.
    UCSCi uc012faj.1. mouse.

    Organism-specific databases

    CTDi 1175.
    MGIi MGI:2141861. Ap2s1.

    Phylogenomic databases

    eggNOGi COG5030.
    HOGENOMi HOG000185227.
    HOVERGENi HBG050517.
    InParanoidi P62743.
    KOi K11827.
    OMAi SFFDNVC.
    OrthoDBi EOG7S7SGC.
    PhylomeDBi P62743.
    TreeFami TF300139.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_202778. Retrograde neurotrophin signalling.
    REACT_203917. EGFR downregulation.
    REACT_222404. WNT5A-dependent internalization of FZD4.

    Miscellaneous databases

    EvolutionaryTracei P62743.
    NextBioi 381325.
    PROi P62743.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62743.
    Bgeei P62743.
    Genevestigatori P62743.

    Family and domain databases

    InterProi IPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR027156. APS2.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view ]
    PANTHERi PTHR11753. PTHR11753. 1 hit.
    PTHR11753:SF6. PTHR11753:SF6. 1 hit.
    Pfami PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Adaptor protein complexes as the key regulators of protein sorting in the post-Golgi network."
      Nakatsu F., Ohno H.
      Cell Struct. Funct. 28:419-429(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE AP-2 COMPLEX IN CLATHRIN-MEDIATED ENDOCYTOSIS.
    3. "Adaptors for clathrin coats: structure and function."
      Owen D.J., Collins B.M., Evans P.R.
      Annu. Rev. Cell Dev. Biol. 20:153-191(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE AP-2 COMPLEX IN CLATHRIN-MEDIATED ENDOCYTOSIS.
    4. "Molecular architecture and functional model of the endocytic AP2 complex."
      Collins B.M., McCoy A.J., Kent H.M., Evans P.R., Owen D.J.
      Cell 109:523-535(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.59 ANGSTROMS) IN COMPLEX WITH AP2B1; AP2M1; AP2A2 AND AN INOSITOL POLYPHOSPHATE HEADGROUP.
    5. "A structural explanation for the binding of endocytic dileucine motifs by the AP2 complex."
      Kelly B.T., McCoy A.J., Spaete K., Miller S.E., Evans P.R., Hoening S., Owen D.J.
      Nature 456:976-979(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.98 ANGSTROMS) IN COMPLEX WITH AP2A2; AP2B1; AP2M1 AND CD4 INTERNALIZATION SIGNAL, MUTAGENESIS OF ARG-15; TYR-62; ALA-63; LEU-65; VAL-88; ASN-92; VAL-98; GLU-100 AND LEU-103.

    Entry informationi

    Entry nameiAP2S1_MOUSE
    AccessioniPrimary (citable) accession number: P62743
    Secondary accession number(s): P70626, P97626, Q00380
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3