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P62738

- ACTA_RAT

UniProt

P62738 - ACTA_RAT

Protein

Actin, aortic smooth muscle

Gene

Acta2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. muscle contraction Source: RGD

    Keywords - Molecular functioni

    Muscle protein

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin, aortic smooth muscle
    Alternative name(s):
    Alpha-actin-2
    Gene namesi
    Name:Acta2
    Synonyms:Actsa, Actvs
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621676. Acta2.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. cytoplasm Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 22Removed in mature formBy similarityPRO_0000000744
    Chaini3 – 377375Actin, aortic smooth musclePRO_0000000745Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31N-acetylglutamateBy similarity
    Modified residuei46 – 461Methionine (R)-sulfoxideBy similarity
    Modified residuei49 – 491Methionine (R)-sulfoxideBy similarity
    Modified residuei75 – 751Tele-methylhistidineBy similarity

    Post-translational modificationi

    Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization By similarity.By similarity
    Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration By similarity.By similarity

    Keywords - PTMi

    Acetylation, Methylation, Oxidation

    Proteomic databases

    PaxDbiP62738.
    PRIDEiP62738.

    Expressioni

    Gene expression databases

    GenevestigatoriP62738.

    Interactioni

    Subunit structurei

    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

    Protein-protein interaction databases

    BioGridi249533. 1 interaction.
    IntActiP62738. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP62738.
    SMRiP62738. Positions 4-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the actin family.Curated

    Phylogenomic databases

    eggNOGiCOG5277.
    HOGENOMiHOG000233340.
    HOVERGENiHBG003771.
    InParanoidiP62738.
    KOiK12313.

    Family and domain databases

    InterProiIPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR004001. Actin_CS.
    [Graphical view]
    PANTHERiPTHR11937. PTHR11937. 1 hit.
    PfamiPF00022. Actin. 1 hit.
    [Graphical view]
    PRINTSiPR00190. ACTIN.
    SMARTiSM00268. ACTIN. 1 hit.
    [Graphical view]
    PROSITEiPS00406. ACTINS_1. 1 hit.
    PS00432. ACTINS_2. 1 hit.
    PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62738-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG    50
    QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH SFYNELRVAP 100
    EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT 150
    TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY 200
    SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI 250
    TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV 300
    LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS 350
    LSTFQQMWIS KQEYDEAGPS IVHRKCF 377
    Length:377
    Mass (Da):42,009
    Last modified:August 16, 2004 - v1
    Checksum:i2D0543262DB35CA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06801 mRNA. Translation: CAA29957.1.
    X00306 mRNA. Translation: CAA25083.1.
    M22757 mRNA. Translation: AAA74457.1.
    PIRiJE0005.
    RefSeqiNP_112266.1. NM_031004.2.
    UniGeneiRn.195319.
    Rn.3114.

    Genome annotation databases

    GeneIDi81633.
    KEGGirno:81633.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06801 mRNA. Translation: CAA29957.1 .
    X00306 mRNA. Translation: CAA25083.1 .
    M22757 mRNA. Translation: AAA74457.1 .
    PIRi JE0005.
    RefSeqi NP_112266.1. NM_031004.2.
    UniGenei Rn.195319.
    Rn.3114.

    3D structure databases

    ProteinModelPortali P62738.
    SMRi P62738. Positions 4-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249533. 1 interaction.
    IntActi P62738. 2 interactions.

    Proteomic databases

    PaxDbi P62738.
    PRIDEi P62738.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81633.
    KEGGi rno:81633.

    Organism-specific databases

    CTDi 59.
    RGDi 621676. Acta2.

    Phylogenomic databases

    eggNOGi COG5277.
    HOGENOMi HOG000233340.
    HOVERGENi HBG003771.
    InParanoidi P62738.
    KOi K12313.

    Miscellaneous databases

    NextBioi 615111.

    Gene expression databases

    Genevestigatori P62738.

    Family and domain databases

    InterProi IPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR004001. Actin_CS.
    [Graphical view ]
    PANTHERi PTHR11937. PTHR11937. 1 hit.
    Pfami PF00022. Actin. 1 hit.
    [Graphical view ]
    PRINTSi PR00190. ACTIN.
    SMARTi SM00268. ACTIN. 1 hit.
    [Graphical view ]
    PROSITEi PS00406. ACTINS_1. 1 hit.
    PS00432. ACTINS_2. 1 hit.
    PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of a rat vascular smooth muscle alpha-actin cDNA."
      McHugh K.M., Lessard J.L.
      Nucleic Acids Res. 16:4167-4167(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Stomach.
    2. "Expression of the genes coding for the skeletal muscle and cardiac actions in the heart."
      Mayer Y., Czosnek H., Zeelon E.P., Yaffe D., Nudel U.
      Nucleic Acids Res. 12:1087-1100(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 285-377.
    3. "Conserved and unique sequences in the 3'-untranslated region of rat smooth-muscle alpha-actin mRNA."
      Hsu C.Y., Frankel F.R.
      Gene 69:345-348(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 336-377.

    Entry informationi

    Entry nameiACTA_RAT
    AccessioniPrimary (citable) accession number: P62738
    Secondary accession number(s): P03996, P04108, P70476
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3