P62738 (ACTA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin, aortic smooth muscle

Alternative name(s):
Alpha-actin-2

Gene names

Name:	Acta2
Synonyms:	Actsa, Actvs

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit structure	Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location	Cytoplasm › cytoskeleton.
Post-translational modification	Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced By similarity.
Miscellaneous	In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Sequence similarities	Belongs to the actin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Muscle protein
PTM	Acetylation Methylation Oxidation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	muscle contraction Inferred from direct assay PubMed 11953441. Source: RGD
Cellular_component	actin cytoskeleton Inferred from direct assay PubMed 8616889. Source: MGI cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 2	2	Removed in mature form By similarity		PRO_0000000744
	Chain	3 – 377	375	Actin, aortic smooth muscle		PRO_0000000745
Amino acid modifications
	Modified residue	3	1	N-acetylglutamate By similarity
	Modified residue	46	1	Methionine sulfoxide By similarity
	Modified residue	75	1	Tele-methylhistidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62738 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 2D0543262DB35CA5	FASTA	377	42,009
	10 20 30 40 50 60 MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA 70 80 90 100 110 120 QSKRGILTLK YPIEHGIITN WDDMEKIWHH SFYNELRVAP EEHPTLLTEA PLNPKANREK 130 140 150 160 170 180 MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL 190 200 210 220 230 240 DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK 250 260 270 280 290 300 SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV 310 320 330 340 350 360 LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIS 370 KQEYDEAGPS IVHRKCF

References

[1]	"The nucleotide sequence of a rat vascular smooth muscle alpha-actin cDNA." McHugh K.M., Lessard J.L. Nucleic Acids Res. 16:4167-4167(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Stomach.
[2]	"Expression of the genes coding for the skeletal muscle and cardiac actions in the heart." Mayer Y., Czosnek H., Zeelon E.P., Yaffe D., Nudel U. Nucleic Acids Res. 12:1087-1100(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 285-377.
[3]	"Conserved and unique sequences in the 3'-untranslated region of rat smooth-muscle alpha-actin mRNA." Hsu C.Y., Frankel F.R. Gene 69:345-348(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 336-377.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06801 mRNA. Translation: CAA29957.1. X00306 mRNA. Translation: CAA25083.1. M22757 mRNA. Translation: AAA74457.1.
IPI	IPI00197129.
PIR	JE0005.
RefSeq	NP_112266.1. NM_031004.2.
UniGene	Rn.195319. Rn.3114.
3D structure databases
ProteinModelPortal	P62738.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62738. 2 interactions.
Proteomic databases
PaxDb	P62738.
PRIDE	P62738.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81633.
KEGG	rno:81633.
Organism-specific databases
CTD	59.
RGD	621676. Acta2.
Phylogenomic databases
eggNOG	COG5277.
HOGENOM	HOG000233340.
HOVERGEN	HBG003771.
InParanoid	P62738.
KO	K12313.
OrthoDB	EOG4W9J40.
Gene expression databases
ArrayExpress	P62738.
Genevestigator	P62738.
GermOnline	ENSRNOG00000008536. Rattus norvegicus.
Family and domain databases
InterPro	IPR004000. Actin-related. IPR020902. Actin/actin-like_CS. IPR004001. Actin_CS. [Graphical view]
PANTHER	PTHR11937. PTHR11937. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. 1 hit. PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615111.

Entry information

Entry name

ACTA_RAT

Accession

Primary (citable) accession number: P62738
Secondary accession number(s): P03996, P04108, P70476

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program