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P62716

- PP2AB_RAT

UniProt

P62716 - PP2AB_RAT

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Protein
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
Gene
Ppp2cb
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Manganese 1 By similarity
Metal bindingi59 – 591Manganese 1 By similarity
Metal bindingi85 – 851Manganese 1 By similarity
Metal bindingi85 – 851Manganese 2 By similarity
Metal bindingi117 – 1171Manganese 2 By similarity
Active sitei118 – 1181Proton donor By similarity
Metal bindingi167 – 1671Manganese 2 By similarity
Metal bindingi241 – 2411Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB
  3. protein C-terminus binding Source: RGD
  4. protein heterodimerization activity Source: UniProtKB
  5. protein serine/threonine phosphatase activity Source: RGD

GO - Biological processi

  1. apoptotic mitochondrial changes Source: Ensembl
  2. negative regulation of Ras protein signal transduction Source: RGD
  3. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  4. protein dephosphorylation Source: RGD
  5. regulation of gene expression Source: Ensembl
  6. response to antibiotic Source: Ensembl
  7. response to endoplasmic reticulum stress Source: Ensembl
  8. response to hydrogen peroxide Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.
REACT_198842. MASTL Facilitates Mitotic Progression.
REACT_199007. Spry regulation of FGF signaling.
REACT_216976. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_225694. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (EC:3.1.3.16)
Short name:
PP2A-beta
Gene namesi
Name:Ppp2cb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 16

Organism-specific databases

RGDi3381. Ppp2cb.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle pole By similarity
Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles By similarity.

GO - Cellular componenti

  1. chromosome, centromeric region Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. protein phosphatase type 2A complex Source: Ensembl
  5. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
PRO_0000058849Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei307 – 3071Phosphotyrosine By similarity
Modified residuei309 – 3091Leucine methyl ester By similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (Lcmt1) and protein phosphatase methylesterase 1 (Ppme1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation By similarity.

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP62716.
PRIDEiP62716.

2D gel databases

World-2DPAGE0004:P62716.

PTM databases

PhosphoSiteiP62716.

Expressioni

Gene expression databases

GenevestigatoriP62716.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1 By similarity. May indirectly interact with SGOL1, most probably through regulatory B56 subunits. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD By similarity. Interacts with CTTNBP2NL By similarity.

Protein-protein interaction databases

BioGridi246805. 1 interaction.
IntActiP62716. 1 interaction.
STRINGi10116.ENSRNOP00000020663.

Structurei

3D structure databases

ProteinModelPortaliP62716.
SMRiP62716. Positions 6-293.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiP62716.
KOiK04382.
OMAiRPPDYFL.
OrthoDBiEOG74N5H2.
PhylomeDBiP62716.
TreeFamiTF105559.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62716-1 [UniParc]FASTAAdd to Basket

« Hide

MDDKAFTKEL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC    50
PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL 100
VALKVRYPER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF 150
DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW 200
SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW 250
CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
TRRTPDYFL 309
Length:309
Mass (Da):35,575
Last modified:July 19, 2004 - v1
Checksum:i51DA9EB0633FC191
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23591 mRNA. Translation: AAA41912.1.
X16044 mRNA. Translation: CAA34167.1.
X14087 mRNA. Translation: CAA32249.1.
M58438 mRNA. Translation: AAA41911.1.
BC085926 mRNA. Translation: AAH85926.1.
M58439 mRNA. Translation: AAA41913.1.
PIRiS08486. PART2B.
RefSeqiNP_058736.1. NM_017040.1.
UniGeneiRn.1271.
Rn.977.

Genome annotation databases

EnsembliENSRNOT00000020663; ENSRNOP00000020663; ENSRNOG00000015182.
GeneIDi24673.
KEGGirno:24673.
UCSCiRGD:3381. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23591 mRNA. Translation: AAA41912.1 .
X16044 mRNA. Translation: CAA34167.1 .
X14087 mRNA. Translation: CAA32249.1 .
M58438 mRNA. Translation: AAA41911.1 .
BC085926 mRNA. Translation: AAH85926.1 .
M58439 mRNA. Translation: AAA41913.1 .
PIRi S08486. PART2B.
RefSeqi NP_058736.1. NM_017040.1.
UniGenei Rn.1271.
Rn.977.

3D structure databases

ProteinModelPortali P62716.
SMRi P62716. Positions 6-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246805. 1 interaction.
IntActi P62716. 1 interaction.
STRINGi 10116.ENSRNOP00000020663.

Chemistry

BindingDBi P62716.

PTM databases

PhosphoSitei P62716.

2D gel databases

World-2DPAGE 0004:P62716.

Proteomic databases

PaxDbi P62716.
PRIDEi P62716.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000020663 ; ENSRNOP00000020663 ; ENSRNOG00000015182 .
GeneIDi 24673.
KEGGi rno:24673.
UCSCi RGD:3381. rat.

Organism-specific databases

CTDi 5516.
RGDi 3381. Ppp2cb.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00550000074618.
HOGENOMi HOG000172696.
HOVERGENi HBG000216.
InParanoidi P62716.
KOi K04382.
OMAi RPPDYFL.
OrthoDBi EOG74N5H2.
PhylomeDBi P62716.
TreeFami TF105559.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.
REACT_198842. MASTL Facilitates Mitotic Progression.
REACT_199007. Spry regulation of FGF signaling.
REACT_216976. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_225694. Glycolysis.

Miscellaneous databases

NextBioi 604049.
PROi P62716.

Gene expression databases

Genevestigatori P62716.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a rat heart cDNA encoding the isotype beta of the catalytic subunit of protein phosphatase 2A."
    Posas F., Arino J.
    Nucleic Acids Res. 17:8370-8370(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "Molecular cloning of rat phosphoprotein phosphatase 2A beta cDNA and increased expressions of phosphatase 2A alpha and 2A beta in rat liver tumors."
    Kitagawa Y., Sakai R., Tahira T., Tsuda H., Ito N., Sugimura T., Nagao M.
    Biochem. Biophys. Res. Commun. 157:821-827(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
    Wadzinski B.E., Heasley L.E., Johnson G.L.
    J. Biol. Chem. 265:21504-21508(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-260.
    Tissue: Hepatoma and Pheochromocytoma.

Entry informationi

Entry nameiPP2AB_RAT
AccessioniPrimary (citable) accession number: P62716
Secondary accession number(s): P11082, Q6LDK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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