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Reviewed, UniProtKB/Swiss-Prot P62716 (PP2AB_RAT)

Last modified November 3, 2009. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
      Short name=PP2A-beta
    EC=3.1.3.16
Gene names
Name: Ppp2cb
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1 By similarity. May indirectly interact with SGOL1, most probably through regulatory B56 subunits By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Centromere By similarity. Cytoplasmcytoskeletonspindle pole By similarity. Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles By similarity.

Post-translational modification

Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro) By similarity.

Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
PRO_0000058849

Sites

Active site1181Proton donor By similarity
Metal binding571Iron By similarity
Metal binding591Iron By similarity
Metal binding851Iron By similarity
Metal binding851Manganese By similarity
Metal binding1171Manganese By similarity
Metal binding1671Manganese By similarity
Metal binding2411Manganese By similarity

Amino acid modifications

Modified residue3071Phosphotyrosine By similarity
Modified residue3091Leucine methyl ester By similarity

Sequences

Sequence LengthMass (Da)Tools
P62716-1 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 51DA9EB0633FC191

FASTA30935,575
        10         20         30         40         50         60 
MDDKAFTKEL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC PVTVCGDVHG 

        70         80         90        100        110        120 
QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYPER ITILRGNHES 

       130        140        150        160        170        180 
RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI 

       190        200        210        220        230        240 
RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA 

       250        260        270        280        290        300 
HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 


TRRTPDYFL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a rat heart cDNA encoding the isotype beta of the catalytic subunit of protein phosphatase 2A."
Posas F., Arino J.
Nucleic Acids Res. 17:8370-8370(1989) [PubMed: 2554256] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"Molecular cloning of rat phosphoprotein phosphatase 2A beta cDNA and increased expressions of phosphatase 2A alpha and 2A beta in rat liver tumors."
Kitagawa Y., Sakai R., Tahira T., Tsuda H., Ito N., Sugimura T., Nagao M.
Biochem. Biophys. Res. Commun. 157:821-827(1988) [PubMed: 2849437] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[4]"Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
Wadzinski B.E., Heasley L.E., Johnson G.L.
J. Biol. Chem. 265:21504-21508(1990) [PubMed: 2174876] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-260.
Tissue: Hepatoma and Pheochromocytoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

M23591 mRNA. Translation: AAA41912.1.
X16044 mRNA. Translation: CAA34167.1.
X14087 mRNA. Translation: CAA32249.1.
M58438 mRNA. Translation: AAA41911.1.
BC085926 mRNA. Translation: AAH85926.1.
M58439 mRNA. Translation: AAA41913.1.
IPIIPI00190462.
PIRPART2B. S08486.
RefSeqNP_058736.1.
UniGeneRn.977

3D structure databases

HSSPHSSP built from PDB template 1FJM based on UniProtKB P08129.
SMRP62716. Positions 2-309.
ModBaseSearch...

Protein-protein interaction databases

STRINGP62716.

PTM databases

PhosphoSiteP62716.

Proteomic databases

PRIDEP62716.

Genome annotation databases

EnsemblENSRNOT00000020663; ENSRNOP00000020663; ENSRNOG00000015182; Rattus norvegicus. [Genome view]
GeneID24673.
KEGGrno:24673.
UCSCNM_017040. rat.

Organism-specific databases

CTD24673.
RGD3381. Ppp2cb.

Phylogenomic databases

HOVERGENP62716.
OMAERLMQCK.

Enzyme and pathway databases

BRENDA3.1.3.16. 248.

Gene expression databases

ArrayExpressP62716.
GenevestigatorP62716.
GermOnlineENSRNOG00000015182. Rattus norvegicus.

Family and domain databases

InterProIPR004843. M-pesterase.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PANTHERPTHR11668. T_phtase_apaH. 1 hit.
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
ProDomPD000252. T_phtase_apaH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio604049.

Entry information

Entry namePP2AB_RAT
AccessionPrimary (citable) accession number: P62716
Secondary accession number(s): P11082, Q6LDK0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents