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P62716

- PP2AB_RAT

UniProt

P62716 - PP2AB_RAT

Protein

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Gene

Ppp2cb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Manganese 1By similarity
    Metal bindingi59 – 591Manganese 1By similarity
    Metal bindingi85 – 851Manganese 1By similarity
    Metal bindingi85 – 851Manganese 2By similarity
    Metal bindingi117 – 1171Manganese 2By similarity
    Active sitei118 – 1181Proton donorBy similarity
    Metal bindingi167 – 1671Manganese 2By similarity
    Metal bindingi241 – 2411Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB
    3. protein C-terminus binding Source: RGD
    4. protein heterodimerization activity Source: UniProtKB
    5. protein serine/threonine phosphatase activity Source: RGD

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: Ensembl
    2. negative regulation of Ras protein signal transduction Source: RGD
    3. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    4. protein dephosphorylation Source: RGD
    5. regulation of gene expression Source: Ensembl
    6. response to antibiotic Source: Ensembl
    7. response to endoplasmic reticulum stress Source: Ensembl
    8. response to hydrogen peroxide Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.
    REACT_198842. MASTL Facilitates Mitotic Progression.
    REACT_199007. Spry regulation of FGF signaling.
    REACT_216976. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_225694. Glycolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (EC:3.1.3.16)
    Short name:
    PP2A-beta
    Gene namesi
    Name:Ppp2cb
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 16

    Organism-specific databases

    RGDi3381. Ppp2cb.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles By similarity.By similarity

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell
    4. protein phosphatase type 2A complex Source: Ensembl
    5. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit beta isoformPRO_0000058849Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei307 – 3071PhosphotyrosineBy similarity
    Modified residuei309 – 3091Leucine methyl esterBy similarity

    Post-translational modificationi

    Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (Lcmt1) and protein phosphatase methylesterase 1 (Ppme1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.By similarity
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation By similarity.By similarity

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiP62716.
    PRIDEiP62716.

    2D gel databases

    World-2DPAGE0004:P62716.

    PTM databases

    PhosphoSiteiP62716.

    Expressioni

    Gene expression databases

    GenevestigatoriP62716.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1 By similarity. May indirectly interact with SGOL1, most probably through regulatory B56 subunits. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD By similarity. Interacts with CTTNBP2NL By similarity.By similarity

    Protein-protein interaction databases

    BioGridi246805. 1 interaction.
    IntActiP62716. 1 interaction.
    STRINGi10116.ENSRNOP00000020663.

    Structurei

    3D structure databases

    ProteinModelPortaliP62716.
    SMRiP62716. Positions 6-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074618.
    HOGENOMiHOG000172696.
    HOVERGENiHBG000216.
    InParanoidiP62716.
    KOiK04382.
    OMAiRPPDYFL.
    OrthoDBiEOG74N5H2.
    PhylomeDBiP62716.
    TreeFamiTF105559.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62716-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDKAFTKEL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC    50
    PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL 100
    VALKVRYPER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF 150
    DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW 200
    SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW 250
    CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
    TRRTPDYFL 309
    Length:309
    Mass (Da):35,575
    Last modified:July 19, 2004 - v1
    Checksum:i51DA9EB0633FC191
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23591 mRNA. Translation: AAA41912.1.
    X16044 mRNA. Translation: CAA34167.1.
    X14087 mRNA. Translation: CAA32249.1.
    M58438 mRNA. Translation: AAA41911.1.
    BC085926 mRNA. Translation: AAH85926.1.
    M58439 mRNA. Translation: AAA41913.1.
    PIRiS08486. PART2B.
    RefSeqiNP_058736.1. NM_017040.1.
    UniGeneiRn.1271.
    Rn.977.

    Genome annotation databases

    EnsembliENSRNOT00000020663; ENSRNOP00000020663; ENSRNOG00000015182.
    GeneIDi24673.
    KEGGirno:24673.
    UCSCiRGD:3381. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23591 mRNA. Translation: AAA41912.1 .
    X16044 mRNA. Translation: CAA34167.1 .
    X14087 mRNA. Translation: CAA32249.1 .
    M58438 mRNA. Translation: AAA41911.1 .
    BC085926 mRNA. Translation: AAH85926.1 .
    M58439 mRNA. Translation: AAA41913.1 .
    PIRi S08486. PART2B.
    RefSeqi NP_058736.1. NM_017040.1.
    UniGenei Rn.1271.
    Rn.977.

    3D structure databases

    ProteinModelPortali P62716.
    SMRi P62716. Positions 6-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246805. 1 interaction.
    IntActi P62716. 1 interaction.
    STRINGi 10116.ENSRNOP00000020663.

    Chemistry

    BindingDBi P62716.

    PTM databases

    PhosphoSitei P62716.

    2D gel databases

    World-2DPAGE 0004:P62716.

    Proteomic databases

    PaxDbi P62716.
    PRIDEi P62716.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000020663 ; ENSRNOP00000020663 ; ENSRNOG00000015182 .
    GeneIDi 24673.
    KEGGi rno:24673.
    UCSCi RGD:3381. rat.

    Organism-specific databases

    CTDi 5516.
    RGDi 3381. Ppp2cb.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074618.
    HOGENOMi HOG000172696.
    HOVERGENi HBG000216.
    InParanoidi P62716.
    KOi K04382.
    OMAi RPPDYFL.
    OrthoDBi EOG74N5H2.
    PhylomeDBi P62716.
    TreeFami TF105559.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.
    REACT_198842. MASTL Facilitates Mitotic Progression.
    REACT_199007. Spry regulation of FGF signaling.
    REACT_216976. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_225694. Glycolysis.

    Miscellaneous databases

    NextBioi 604049.
    PROi P62716.

    Gene expression databases

    Genevestigatori P62716.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a rat heart cDNA encoding the isotype beta of the catalytic subunit of protein phosphatase 2A."
      Posas F., Arino J.
      Nucleic Acids Res. 17:8370-8370(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "Molecular cloning of rat phosphoprotein phosphatase 2A beta cDNA and increased expressions of phosphatase 2A alpha and 2A beta in rat liver tumors."
      Kitagawa Y., Sakai R., Tahira T., Tsuda H., Ito N., Sugimura T., Nagao M.
      Biochem. Biophys. Res. Commun. 157:821-827(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    4. "Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
      Wadzinski B.E., Heasley L.E., Johnson G.L.
      J. Biol. Chem. 265:21504-21508(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-260.
      Tissue: Hepatoma and Pheochromocytoma.

    Entry informationi

    Entry nameiPP2AB_RAT
    AccessioniPrimary (citable) accession number: P62716
    Secondary accession number(s): P11082, Q6LDK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3